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Q64429

- CP1B1_MOUSE

UniProt

Q64429 - CP1B1_MOUSE

Protein

Cytochrome P450 1B1

Gene

Cyp1b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, retinoid and xenobiotics. Preferentially oxidizes 17beta-estradiol to the carcinogenic 4-hydroxy derivative, and a variety of procarcinogenic compounds to their activated forms, including polycyclic aromatic hydrocarbons. Promotes angiogenesis by removing cellular oxygenation products, thereby decreasing oxidative stress, release of antiangiogenic factor THBS2, then allowing endothelial cells migration, cell adhesion and capillary morphogenesis. These changes are concommitant with the endothelial nitric oxide synthase activity and nitric oxide synthesis. Plays an important role in the regulation of perivascular cell proliferation, migration, and survival through modulation of the intracellular oxidative state and NF-kappa-B expression and/or activity, during angiogenesis. Contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression.6 Publications

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Enzyme regulationi

    Activated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Inhibited by 12-O-Tetradecanoylphorbol-13-acetate and PRKCB.1 Publication

    Kineticsi

    kcat is 0.04 min(-1) for retinol, 0.08 min(-1) for retinal, 1.24 min(-1) for 7,12-dimethyltetraphene, 0.13 min(-1) for arachidonic acid.

    1. KM=392.2 µM for retinol1 Publication
    2. KM=153.9 µM for retinal1 Publication
    3. KM=138.9 µM for 7,12-dimethyltetraphene1 Publication
    4. KM=500.0 µM for arachidonic acid1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi470 – 4701Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: UniProtKB
    3. iron ion binding Source: InterPro
    4. monooxygenase activity Source: UniProtKB
    5. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: MGI

    GO - Biological processi

    1. angiogenesis Source: MGI
    2. arachidonic acid metabolic process Source: UniProtKB
    3. blood vessel morphogenesis Source: UniProtKB
    4. cell adhesion Source: UniProtKB
    5. cellular aromatic compound metabolic process Source: MGI
    6. cellular response to hydrogen peroxide Source: UniProtKB
    7. cellular response to organic cyclic compound Source: MGI
    8. collagen fibril organization Source: UniProtKB
    9. endothelial cell-cell adhesion Source: MGI
    10. endothelial cell migration Source: UniProtKB
    11. estrogen metabolic process Source: UniProtKB
    12. intrinsic apoptotic signaling pathway in response to oxidative stress Source: UniProtKB
    13. membrane lipid catabolic process Source: UniProtKB
    14. negative regulation of cell adhesion mediated by integrin Source: UniProtKB
    15. negative regulation of cell migration Source: UniProtKB
    16. negative regulation of cell proliferation Source: UniProtKB
    17. negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
    18. nitric oxide biosynthetic process Source: UniProtKB
    19. positive regulation of angiogenesis Source: UniProtKB
    20. positive regulation of apoptotic process Source: UniProtKB
    21. positive regulation of gene expression involved in extracellular matrix organization Source: UniProtKB
    22. positive regulation of JAK-STAT cascade Source: UniProtKB
    23. positive regulation vascular endothelial growth factor production Source: UniProtKB
    24. regulation of reactive oxygen species metabolic process Source: UniProtKB
    25. response to toxic substance Source: MGI
    26. retinal blood vessel morphogenesis Source: UniProtKB
    27. retinal metabolic process Source: UniProtKB
    28. retina vasculature development in camera-type eye Source: MGI
    29. retinol metabolic process Source: UniProtKB
    30. steroid metabolic process Source: UniProtKB
    31. toxin metabolic process Source: MGI
    32. trabecular meshwork development Source: UniProtKB
    33. xenobiotic metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196570. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
    REACT_196572. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
    REACT_227038. Endogenous sterols.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 1B1 (EC:1.14.14.1)
    Alternative name(s):
    CYPIB1
    Cytochrome P450CMEF
    Short name:
    Cytochrome P450EF
    Gene namesi
    Name:Cyp1b1
    Synonyms:Cyp1-b1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:88590. Cyp1b1.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication. Microsome membrane 1 Publication; Peripheral membrane protein 1 Publication. Mitochondrion 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome, Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Severe ocular drainage structure abnormalities, significant elevated intraocular pressure.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 543543Cytochrome P450 1B1PRO_0000051661Add
    BLAST

    Proteomic databases

    PaxDbiQ64429.
    PRIDEiQ64429.

    PTM databases

    PhosphoSiteiQ64429.

    Expressioni

    Tissue specificityi

    Constitutively expressed in retinal, heart and kidney pericytes cells.1 Publication

    Inductioni

    By polycyclic aromatic hydrocarbons (PAH) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).1 Publication

    Gene expression databases

    ArrayExpressiQ64429.
    BgeeiQ64429.
    GenevestigatoriQ64429.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64429.
    SMRiQ64429. Positions 68-530.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117317.
    HOGENOMiHOG000036991.
    HOVERGENiHBG106944.
    InParanoidiQ3UVA8.
    KOiK07410.
    OMAiNEPSKMS.
    OrthoDBiEOG7RBZ85.
    TreeFamiTF105095.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64429-1 [UniParc]FASTAAdd to Basket

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    MATSLSADSP QQLSSLSTQQ TTLLLLFSVL AAVHLGQWLL RQWQRKPWSS    50
    PPGPFPWPLI GNAAAVGQAS HLYFARLARR YGDVFQIRLG SCPVVVLNGE 100
    SAIHQALVQQ GSIFADRPPF ASFRVVSGGR SLAFGHYSEH WKTQRRSAYS 150
    TMRAFSTRHP RSRGLLEGHA LAEARELVAV LVRRCAGGAF LDPTQPVIVA 200
    VANVMSAVCF GCRYNHDDAE FLELLSHNEE FGRTVGAGSL VDVLPWLQLF 250
    PNPVRTTFRK FEQLNRNFSN FVLDKFLRHR ESLVPGAAPR DMTDAFILSA 300
    EKKASGAPGD DSSGLDLEDV PATITDIFGA SQDTLSTALL WLLILFTRYP 350
    DVQARVQAEL DQVVGRDRLP CMSDQPNLPY VMAFLYESMR FSSFLPVTIP 400
    HATTANTFVL GYYIPKNTVV FVNQWSVNHD PAKWPNPEDF DPARFLDKDG 450
    FINKALASSV MIFSVGKRRC IGEELSKMLL FLFISILAHQ CNFKANQNES 500
    SNMSFSYGLT IKPKSFRIHV SLRESMELLD NAVKKLQTEE GCK 543
    Length:543
    Mass (Da):60,537
    Last modified:July 27, 2011 - v3
    Checksum:i7C89B4312CB5BC4A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti195 – 1951Q → P in CAA55205. (PubMed:8195121)Curated
    Sequence conflicti307 – 3071A → D in CAA55205. (PubMed:8195121)Curated
    Sequence conflicti328 – 3281F → G in CAA55205. (PubMed:8195121)Curated
    Sequence conflicti457 – 4571A → T in CAA55205. (PubMed:8195121)Curated
    Sequence conflicti516 – 5161F → V in CAA55205. (PubMed:8195121)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03283 mRNA. Translation: AAC52141.1.
    X78445 mRNA. Translation: CAA55205.1.
    AK137461 mRNA. Translation: BAE23362.1.
    CH466537 Genomic DNA. Translation: EDL38490.1.
    U02479 mRNA. Translation: AAC52131.1.
    CCDSiCCDS28986.1.
    PIRiA53790.
    RefSeqiNP_034124.1. NM_009994.1.
    UniGeneiMm.214016.

    Genome annotation databases

    EnsembliENSMUST00000024894; ENSMUSP00000024894; ENSMUSG00000024087.
    GeneIDi13078.
    KEGGimmu:13078.
    UCSCiuc008dqc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03283 mRNA. Translation: AAC52141.1 .
    X78445 mRNA. Translation: CAA55205.1 .
    AK137461 mRNA. Translation: BAE23362.1 .
    CH466537 Genomic DNA. Translation: EDL38490.1 .
    U02479 mRNA. Translation: AAC52131.1 .
    CCDSi CCDS28986.1.
    PIRi A53790.
    RefSeqi NP_034124.1. NM_009994.1.
    UniGenei Mm.214016.

    3D structure databases

    ProteinModelPortali Q64429.
    SMRi Q64429. Positions 68-530.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q64429.

    Proteomic databases

    PaxDbi Q64429.
    PRIDEi Q64429.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024894 ; ENSMUSP00000024894 ; ENSMUSG00000024087 .
    GeneIDi 13078.
    KEGGi mmu:13078.
    UCSCi uc008dqc.1. mouse.

    Organism-specific databases

    CTDi 1545.
    MGIi MGI:88590. Cyp1b1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117317.
    HOGENOMi HOG000036991.
    HOVERGENi HBG106944.
    InParanoidi Q3UVA8.
    KOi K07410.
    OMAi NEPSKMS.
    OrthoDBi EOG7RBZ85.
    TreeFami TF105095.

    Enzyme and pathway databases

    Reactomei REACT_196570. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
    REACT_196572. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
    REACT_227038. Endogenous sterols.

    Miscellaneous databases

    NextBioi 283028.
    PROi Q64429.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64429.
    Bgeei Q64429.
    Genevestigatori Q64429.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning, sequence analysis, and induction by aryl hydrocarbons of a murine cytochrome P450 gene, Cyp1b1."
      Shen Z., Liu J., Wells R.L., Elkind M.M.
      DNA Cell Biol. 13:763-769(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H.
    2. "Mouse cytochrome P-450EF, representative of a new 1B subfamily of cytochrome P-450s. Cloning, sequence determination, and tissue expression."
      Savas U., Bhattacharyya K.K., Christou M., Alexander D.L., Jefcoate C.R.
      J. Biol. Chem. 269:14905-14911(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Bone.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Identification of a cytochrome P450 gene by reverse transcription-PCR using degenerate primers containing inosine."
      Shen Z., Wells R.L., Liu J., Elkind M.M.
      Proc. Natl. Acad. Sci. U.S.A. 90:11483-11487(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 361-466.
    6. "Regulation of cytochrome P4501B1 (CYP1B1) in mouse embryo fibroblast (C3H10T1/2) cells by protein kinase C (PKC)."
      Ikegwuonu F.I., Christou M., Jefcoate C.R.
      Biochem. Pharmacol. 57:619-630(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
    7. "Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1b1."
      Choudhary D., Jansson I., Stoilov I., Sarfarazi M., Schenkman J.B.
      Drug Metab. Dispos. 32:840-847(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    8. "CYP1B1 expression promotes the proangiogenic phenotype of endothelium through decreased intracellular oxidative stress and thrombospondin-2 expression."
      Tang Y., Scheef E.A., Wang S., Sorenson C.M., Marcus C.B., Jefcoate C.R., Sheibani N.
      Blood 113:744-754(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN VASCULAR DEVELOPMENT AND ANGIOGENESIS.
    9. "CYP1B1 and endothelial nitric oxide synthase combine to sustain proangiogenic functions of endothelial cells under hyperoxic stress."
      Tang Y., Scheef E.A., Gurel Z., Sorenson C.M., Jefcoate C.R., Sheibani N.
      Am. J. Physiol. 298:C665-C678(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN ANGIOGENESIS.
    10. Cited for: SUBCELLULAR LOCATION.
    11. "Lack of Cyp1b1 promotes the proliferative and migratory phenotype of perivascular supporting cells."
      Palenski T.L., Sorenson C.M., Jefcoate C.R., Sheibani N.
      Lab. Invest. 93:646-662(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN VASCULAR HOMEOSTASIS, TISSUE SPECIFICITY, INDUCTION.
    12. "Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress."
      Zhao Y., Wang S., Sorenson C.M., Teixeira L., Dubielzig R.R., Peters D.M., Conway S.J., Jefcoate C.R., Sheibani N.
      Mol. Cell. Biol. 33:4225-4240(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN TRABECULAR MESHWORK DEVELOPMENT, DISRUPTION PHENOTYPE.
    13. "Specificity determinants of CYP1B1 estradiol hydroxylation."
      Nishida C.R., Everett S., Ortiz de Montellano P.R.
      Mol. Pharmacol. 84:451-458(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN ESTROGEN METABOLISM.

    Entry informationi

    Entry nameiCP1B1_MOUSE
    AccessioniPrimary (citable) accession number: Q64429
    Secondary accession number(s): Q3UVA8, Q60593, Q64461
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 122 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3