Reviewed, UniProtKB/Swiss-Prot Q64421 (3BHS2_MESAU)

Names and origin

Protein names

Recommended name:
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Alternative name(s):
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
      Short name=3-beta-HSD II
Including the following 2 domains:
    1- Recommended name:
            3-beta-hydroxy-Delta(5)-steroid dehydrogenase
              EC=1.1.1.145
        Alternative name(s):
            3-beta-hydroxy-5-ene steroid dehydrogenase
            Progesterone reductase
    2- Recommended name:
            Steroid Delta-isomerase
              EC=5.3.3.1
        Alternative name(s):
            Delta-5-3-ketosteroid isomerase

Gene names

Name:

HSD3B2

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Also able to act as 3-keto-reductase reducing 5-alpha-dihydrotestosterone to the corresponding 3-beta-androstan-3-beta,17-beta-diol.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Tissue specificity	High levels in adrenal, kidney and male liver. Low levels in female liver.
Sequence similarities	Belongs to the 3-beta-HSD family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 373	372	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2		PRO_0000087778
Regions
	Transmembrane	288 – 308	21	Potential
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	159	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64421-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: FE2B53A62717897A	FASTA	373	41,903
	10 20 30 40 50 60 MPGWSCLVTG AGGFLGQRII HLLVQEKDLE EVRLLDKVFR PETREEFFKL QTKTKVTVLE 70 80 90 100 110 120 GDILDAQCLR RACQGISVVI HTAAAIDVWG IIPRQTIIDI NVKGTLNLLE ACVQASVPAF 130 140 150 160 170 180 IYTSSIDVAG PNSYKEIVLN GHEEQQHEST WSDPYPYSKM MAEKAVLAAN GSFLKNGGTL 190 200 210 220 230 240 HTCALRPMYI YGEKSSILSG IMIRAIKNNG ILKVTGKFST VNPVYVSNAA WAHILAARGL 250 260 270 280 290 300 QDPKKSPNIQ GQFYYISDDT PHQSYDDLNN TLSKKWGLRP DSSWRPPVAL LYWLGFLLEL 310 320 330 340 350 360 VNFLLRPVYN YQPPFTRYLV TISNTVFTFS YKKAQRDLGY EPLVGWEEAR ENTSEWIGSL 370 VEQHKGTLNT KAQ

References

[1]

"Expression and characterization of isoforms of 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase in the hamster." Rogerson F.M., Lehoux J.-G., Mason J.I. J. Steroid Biochem. Mol. Biol. 55:481-487(1995) [PubMed: 8547173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney and Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L38710 mRNA. Translation: AAA96606.1.
3D structure databases
SMR	Q64421. Positions 5-364.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q64421.
Enzyme and pathway databases
BRENDA	1.1.1.145. 824. 5.3.3.1. 824.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

3BHS2_MESAU

Accession

Primary (citable) accession number: Q64421

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 68 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)