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Q64410

- CP17A_CAVPO

UniProt

Q64410 - CP17A_CAVPO

Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

CYP17A1

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

    Catalytic activityi

    A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
    17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

    Cofactori

    Heme group.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi442 – 4421Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. 17-alpha-hydroxyprogesterone aldolase activity Source: UniProtKB-EC
    2. heme binding Source: UniProtKB
    3. iron ion binding Source: InterPro
    4. steroid 17-alpha-monooxygenase activity Source: UniProtKB

    GO - Biological processi

    1. glucocorticoid biosynthetic process Source: Ensembl
    2. hormone biosynthetic process Source: UniProtKB
    3. progesterone metabolic process Source: UniProtKB
    4. steroid metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BRENDAi1.14.99.9. 1225.
    UniPathwayiUPA00062.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
    Alternative name(s):
    17-alpha-hydroxyprogesterone aldolase
    CYPXVII
    Cytochrome P450 17A1
    Cytochrome P450-C17
    Short name:
    Cytochrome P450c17
    Gene namesi
    Name:CYP17A1
    Synonyms:CYP17
    OrganismiCavia porcellus (Guinea pig)
    Taxonomic identifieri10141 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
    ProteomesiUP000005447: Unplaced

    Subcellular locationi

    Membrane Curated

    GO - Cellular componenti

    1. axon Source: Ensembl
    2. membrane Source: UniProtKB-SubCell
    3. mitochondrion Source: Ensembl
    4. neuronal cell body Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 508508Steroid 17-alpha-hydroxylase/17,20 lyasePRO_0000051928Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi10141.ENSCPOP00000013107.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64410.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117317.
    HOGENOMiHOG000036991.
    HOVERGENiHBG106944.
    InParanoidiQ64410.
    OrthoDBiEOG7RBZ85.
    TreeFamiTF105095.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64410-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWELVTLLGL ILAYLFWPRQ GSSGTKYPKS LPSLPVVGSL PFLPKSGHMH    50
    VNFFKLQKKY GPIYSFRLGS TTTVVIGHHQ LARELLIKKG KEFSGRPLTT 100
    TVALLSDNGK GIAFADSSAT WQLHRRLVLS SFSLFRDGEQ KLENIICQEL 150
    SALCDFLATC DGQVKDLSSS IFMTVVNIIC MICFSVSYKE GDMELVTIRR 200
    FTTGFVNSLS DDNLVDIFPW LKIFPNKTLE MIRKYTEIRG AMLSKILKEC 250
    KEKFRSDSVS NLIDLLIQAK VNENNNNSSL DQDSNLFSDK HILTTLGDIF 300
    GAGVETSSSV VLWVIAFLLH NPQVKKKIQE EIDHNVGFSR TPTFSDRNHL 350
    LMLEATIREV LRIRPVAPIL IPHKANTDSS IGEFAIDKDT NVLVNLWALH 400
    HNEQEWDRPD QFMPERFLDP TGSQIIVPSS SYLPFGAGPR SCVGEALARQ 450
    EIFLITAWLL QKFDLEVPEG GQLPSLEGIP KIVFLIDPFK VKITVRPAWK 500
    EAQAEGSA 508
    Length:508
    Mass (Da):57,026
    Last modified:November 1, 1996 - v1
    Checksum:i5F7D6EBFB5B5CC8E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti62 – 621P → S in AAB33048. (PubMed:7811386)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82878 Genomic DNA. Translation: CAA58059.1.
    S75277 mRNA. Translation: AAB33048.1.
    PIRiI53018.
    S52756.
    RefSeqiXP_003475015.1. XM_003474967.1.

    Genome annotation databases

    EnsembliENSCPOT00000014691; ENSCPOP00000013107; ENSCPOG00000014546.
    GeneIDi100729670.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82878 Genomic DNA. Translation: CAA58059.1 .
    S75277 mRNA. Translation: AAB33048.1 .
    PIRi I53018.
    S52756.
    RefSeqi XP_003475015.1. XM_003474967.1.

    3D structure databases

    ProteinModelPortali Q64410.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10141.ENSCPOP00000013107.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSCPOT00000014691 ; ENSCPOP00000013107 ; ENSCPOG00000014546 .
    GeneIDi 100729670.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117317.
    HOGENOMi HOG000036991.
    HOVERGENi HBG106944.
    InParanoidi Q64410.
    OrthoDBi EOG7RBZ85.
    TreeFami TF105095.

    Enzyme and pathway databases

    UniPathwayi UPA00062 .
    BRENDAi 1.14.99.9. 1225.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning of guinea pig adrenal CYP17."
      Huang Y., Voigt J.M., Colby H.D.
      Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: English short hair.
      Tissue: Adrenal cortex.
    2. "Molecular cloning and expression of guinea pig cytochrome P450c17 cDNA (steroid 17 alpha-hydroxylase/17,20 lyase): tissue distribution, regulation, and substrate specificity of the expressed enzyme."
      Tremblay Y., Fleury A., Beaudoin C., Vallee M., Belanger A.
      DNA Cell Biol. 13:1199-1212(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Adrenal gland.
    3. "Partial amino acid sequences of two mitochondrial and two microsomal cytochrome P-450's from adrenal cortex."
      Ogishima T., Okada Y., Kominami S., Takemori S., Omura T.
      J. Biochem. 94:1711-1714(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-15.

    Entry informationi

    Entry nameiCP17A_CAVPO
    AccessioniPrimary (citable) accession number: Q64410
    Secondary accession number(s): Q64659
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3