Reviewed, UniProtKB/Swiss-Prot Q64410 (CP17A_CAVPO)

Names and origin

Protein names

Recommended name:
    Steroid 17-alpha-hydroxylase/17,20 lyase
    EC=1.14.99.9
Alternative name(s):
    Cytochrome P450 17A1
    CYPXVII
    Cytochrome P450-C17
      Short name=Cytochrome P450c17

Gene names

Name:	CYP17A1
Synonyms:	CYP17

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
Catalytic activity	A steroid + AH2 + O2 = a 17-alpha-hydroxysteroid + A + H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Membrane Potential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid 17-alpha-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	Steroid 17-alpha-hydroxylase/17,20 lyase		PRO_0000051928
Sites
	Metal binding	442	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	62	1	P → S in AAB33048. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q64410-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5F7D6EBFB5B5CC8E	FASTA	508	57,026
	10 20 30 40 50 60 MWELVTLLGL ILAYLFWPRQ GSSGTKYPKS LPSLPVVGSL PFLPKSGHMH VNFFKLQKKY 70 80 90 100 110 120 GPIYSFRLGS TTTVVIGHHQ LARELLIKKG KEFSGRPLTT TVALLSDNGK GIAFADSSAT 130 140 150 160 170 180 WQLHRRLVLS SFSLFRDGEQ KLENIICQEL SALCDFLATC DGQVKDLSSS IFMTVVNIIC 190 200 210 220 230 240 MICFSVSYKE GDMELVTIRR FTTGFVNSLS DDNLVDIFPW LKIFPNKTLE MIRKYTEIRG 250 260 270 280 290 300 AMLSKILKEC KEKFRSDSVS NLIDLLIQAK VNENNNNSSL DQDSNLFSDK HILTTLGDIF 310 320 330 340 350 360 GAGVETSSSV VLWVIAFLLH NPQVKKKIQE EIDHNVGFSR TPTFSDRNHL LMLEATIREV 370 380 390 400 410 420 LRIRPVAPIL IPHKANTDSS IGEFAIDKDT NVLVNLWALH HNEQEWDRPD QFMPERFLDP 430 440 450 460 470 480 TGSQIIVPSS SYLPFGAGPR SCVGEALARQ EIFLITAWLL QKFDLEVPEG GQLPSLEGIP 490 500 KIVFLIDPFK VKITVRPAWK EAQAEGSA

References

[1]	"cDNA cloning of guinea pig adrenal CYP17." Huang Y., Voigt J.M., Colby H.D. Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: English short hair. Tissue: Adrenal cortex.
[2]	"Molecular cloning and expression of guinea pig cytochrome P450c17 cDNA (steroid 17 alpha-hydroxylase/17,20 lyase): tissue distribution, regulation, and substrate specificity of the expressed enzyme." Tremblay Y., Fleury A., Beaudoin C., Vallee M., Belanger A. DNA Cell Biol. 13:1199-1212(1994) [PubMed: 7811386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.
[3]	"Partial amino acid sequences of two mitochondrial and two microsomal cytochrome P-450's from adrenal cortex." Ogishima T., Okada Y., Kominami S., Takemori S., Omura T. J. Biochem. 94:1711-1714(1983) [PubMed: 6654880] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X82878 Genomic DNA. Translation: CAA58059.1. S75277 mRNA. Translation: AAB33048.1.
PIR	I53018. S52756.
3D structure databases
SMR	Q64410. Positions 14-496.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCPOT00000014691; ENSCPOP00000013107; ENSCPOG00000014546; Cavia porcellus. [Genome view]
Phylogenomic databases
eggNOG	roNOG05560.
HOVERGEN	Q64410.
InParanoid	Q64410.
OrthoDB	EOG9KD95R.
Enzyme and pathway databases
BRENDA	1.14.99.9. 44. 4.1.2.30. 44.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP17A_CAVPO

Accession

Primary (citable) accession number: Q64410
Secondary accession number(s): Q64659

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)