Reviewed, UniProtKB/Swiss-Prot Q64408 (C11B1_CAVPO)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 11B1, mitochondrial
    EC=1.14.15.4
Alternative name(s):
    CYPXIB1
    P450C11
    Steroid 11-beta-hydroxylase
    Aldosterone synthase

Gene names

Name:

CYP11B1

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has steroid 11-beta-hydroxylase activity. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB.
Catalytic activity	A steroid + reduced adrenal ferredoxin + O(2) = an 11-beta-hydroxysteroid + oxidized adrenal ferredoxin + H(2)O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro steroid 11-beta-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion
	Chain	25 – 500	476	Cytochrome P450 11B1, mitochondrial		PRO_0000003595
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64408-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C4B5AB6041CD4AF2	FASTA	500	57,820
	10 20 30 40 50 60 MAFRLKSDVR LAGSWLCLRG ARALGTRAVT ASKASVLPFE VIPQHQGNKR QRVLQFWKEQ 70 80 90 100 110 120 NHDDLHLEMH QTFQELGPIF RCDVGSTRIV AVMLPEDCAR LHQAESPYPH RMHLEPWMAY 130 140 150 160 170 180 REHRRQNLGV FLLNGPEWLS NRRWLNPNVL SPKAVQNLLP MVDTVARDFS EALKQKVLQS 190 200 210 220 230 240 AQGSLTMDMQ PDIHKYTVEV SNFALFGERL GLFGCNPSSQ SLKFIHALEA VFKTTTQLMF 250 260 270 280 290 300 LPRSLSRWMR SQAWKEHFEA WDYISEYAEN RIQKKYEELA RGCSQYNSIV ANLMLQGNLP 310 320 330 340 350 360 LRAMKANIMD LVAGSVDTTA LPLMMTLFEL ARNPTVQQAL RQESMATEPN IYENPQRLRM 370 380 390 400 410 420 ELPLLWAAIK ETLRMYPVGL FLERFLTSPL VLQNYHIPAG TLVHLNLYSM GRNPEVFLSP 430 440 450 460 470 480 EHYNPQRWLE NKETYKHLAF GFGVRQCIGR RLAEVEMLLF LHHVLKSFCV ETAFQEDVKF 490 500 AYRFVMMPTS APLLTFRPVS

References

[1]

"Molecular cloning and functional expression of the cytochrome P450 11B-hydroxylase of the guinea pig." Buelow H.E., Mobius K., Bahr V., Bernhardt R. Biochem. Biophys. Res. Commun. 221:304-312(1996) [PubMed: 8619851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Dunkin-Hartley. Tissue: Adrenal gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z69785 mRNA. Translation: CAA93633.1.
PIR	JC4709.
3D structure databases
HSSP	HSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCPOG00000013558. Cavia porcellus. [Contig view]
Phylogenomic databases
HOVERGEN	Q64408.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C11B1_CAVPO

Accession

Primary (citable) accession number: Q64408

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)