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Q64398

- DGKH_MESAU

UniProt

Q64398 - DGKH_MESAU

Protein

Diacylglycerol kinase eta

Gene

DGKH

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization By similarity.By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri169 – 21951Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri241 – 29252Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase eta (EC:2.7.1.107)
    Short name:
    DAG kinase eta
    Alternative name(s):
    130 kDa diacylglycerol kinase
    Diglyceride kinase eta
    Short name:
    DGK-eta
    Gene namesi
    Name:DGKH
    OrganismiMesocricetus auratus (Golden hamster)
    Taxonomic identifieri10036 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

    Subcellular locationi

    Cytoplasm. Cell membrane By similarity
    Note: Translocated from the cytoplasm to endosomes in response to stress stimuli. Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11541154Diacylglycerol kinase etaPRO_0000218463Add
    BLAST

    Post-translational modificationi

    Phosphorylated; does not inhibit catalytic activity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Expressed in a wide variety of tissues. Most abundant in the brain and testis; also found in lung, spleen, and prostate.

    Inductioni

    By glucocorticoids.

    Interactioni

    Subunit structurei

    Forms homooligomers through the SAM domain. Able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization inhibits its catalytic activity. Interacts with RAF1 and BRAF By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ64398.
    SMRiQ64398. Positions 222-286.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini59 – 15294PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini322 – 457136DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri169 – 21951Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri241 – 29252Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    HOVERGENiHBG051346.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00233. PH. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64398-1 [UniParc]FASTAAdd to Basket

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    MAGAGYQHHP PGGAAVGTSA VSPTAAGPGE DSSDSEAEQG GPQKLIRKVS     50
    TSGQIRTKTS IKEGQLLKQT SSFQRWKKRY FKLRGRTLYY AKDSKSLIFD 100
    EVDLSDASVA EASTKNANNS FTIITPFRRL MLCAENRKEM EDWISSLKSV 150
    QSREPYEVAQ FNVEHFSGMH NWYACSHARP TFCNVCRESL SGVTSHGLSC 200
    EVCKFKAHKR CAVRATNNCK WTTLASIGKD IIEDEDGVAM PHQWLEGNLP 250
    VSAKCAVCDK TCGSVLRLQD WKCLWCKAMV HTACKDLYHP VCPLGQCKVS 300
    IIPPIALNST DSDGFCRATF SFCVSPLLVF VNSKSGDNQG VKFLRRFKQL 350
    LNPAQVFDLM NGGPHLGLRL FQKFDNFRIL VCGGDGSVGW VLSEIDKLNL 400
    NKQCQLGVLP LGTGNDLARV LGWGGSYDDD TQLPQILEKL ERASTKMLDR 450
    WSIMTYELKL PAKASLLPEP PEASGGFYMT IYEDSVANHL TKILNSDEHA 500
    VVISSAKILC ETVKDFVAKV EKAQDKTLEN TVVAEAVANK CSVLNEKLEQ 550
    LLQALHADAQ ASRVPPGVGP AIPEEDAVES SSEESLGESK DQLVNDIAKP 600
    SSQKAVKPRE IMLRANSLKK AVRQVIEEAG KVMDEQTVQP CEPVSPSCDY 650
    DSPEADDSKD NDTKESPAAK STSQAPEAQA IRGHFQTDSV AGSAMATTKE 700
    NLPVLNTRII CPGLRAGLAA SIAGSSIINK MLLANIDPFG ATPFIDPDLD 750
    SLDGYSEKCV MNNYFGIGLD AKISLEFNNK REEHPEKCRS RTKNLMWYGV 800
    LGTRELLQRS YKNLEQRVQL ECDGQYIPLP SLQGIAVLNI PSYAGGTNFW 850
    GGTKEDDIFA APSFDDKILE VVAVFDSVQM AVSRVIKLQH HRIAQCRTVK 900
    ITIFGDEGVP VQVDGEAWVQ PPGIIKIVHK NRAQMLTRDR AFESTLKSWE 950
    DKQKCDSGKP VLRTNLYIHP AADLATEEVS QMRLCSQAAE ELITRICDAA 1000
    TIHCLLGAGT GSSVNACSHA LNKANPRFPE SLTRDTATEI AINVKALYNE 1050
    TESLLVGRVP LQLESPHEER VSSALHSVEV ELQKLTEIPW LYYILRPNED 1100
    EEPPMDCTKR NSKSTVFRIV PKFKMEKAQK QKTSSQPGPG DTESGSYEAN 1150
    SPGN 1154
    Length:1,154
    Mass (Da):126,818
    Last modified:November 1, 1996 - v1
    Checksum:i0507DEECF64BC5CB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U59429 mRNA. Translation: AAC52714.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U59429 mRNA. Translation: AAC52714.1 .

    3D structure databases

    ProteinModelPortali Q64398.
    SMRi Q64398. Positions 222-286.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG051346.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00233. PH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a glucocorticoid-induced diacylglycerol kinase."
      Klauck T.M., Xu X., Mousseau B., Jaken S.
      J. Biol. Chem. 271:19781-19788(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Smooth muscle.

    Entry informationi

    Entry nameiDGKH_MESAU
    AccessioniPrimary (citable) accession number: Q64398
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3