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Q64398

- DGKH_MESAU

UniProt

Q64398 - DGKH_MESAU

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Protein

Diacylglycerol kinase eta

Gene

DGKH

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization (By similarity).By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri169 – 21951Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri241 – 29252Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase eta (EC:2.7.1.107)
Short name:
DAG kinase eta
Alternative name(s):
130 kDa diacylglycerol kinase
Diglyceride kinase eta
Short name:
DGK-eta
Gene namesi
Name:DGKH
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

Cytoplasm. Cell membrane By similarity
Note: Translocated from the cytoplasm to endosomes in response to stress stimuli. Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11541154Diacylglycerol kinase etaPRO_0000218463Add
BLAST

Post-translational modificationi

Phosphorylated; does not inhibit catalytic activity.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Expressed in a wide variety of tissues. Most abundant in the brain and testis; also found in lung, spleen, and prostate.

Inductioni

By glucocorticoids.

Interactioni

Subunit structurei

Forms homooligomers through the SAM domain. Able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization inhibits its catalytic activity. Interacts with RAF1 and BRAF (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ64398.
SMRiQ64398. Positions 222-286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 15294PHPROSITE-ProRule annotationAdd
BLAST
Domaini322 – 457136DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri169 – 21951Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri241 – 29252Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG051346.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. PE/DAG-bd.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64398-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MAGAGYQHHP PGGAAVGTSA VSPTAAGPGE DSSDSEAEQG GPQKLIRKVS
60 70 80 90 100
TSGQIRTKTS IKEGQLLKQT SSFQRWKKRY FKLRGRTLYY AKDSKSLIFD
110 120 130 140 150
EVDLSDASVA EASTKNANNS FTIITPFRRL MLCAENRKEM EDWISSLKSV
160 170 180 190 200
QSREPYEVAQ FNVEHFSGMH NWYACSHARP TFCNVCRESL SGVTSHGLSC
210 220 230 240 250
EVCKFKAHKR CAVRATNNCK WTTLASIGKD IIEDEDGVAM PHQWLEGNLP
260 270 280 290 300
VSAKCAVCDK TCGSVLRLQD WKCLWCKAMV HTACKDLYHP VCPLGQCKVS
310 320 330 340 350
IIPPIALNST DSDGFCRATF SFCVSPLLVF VNSKSGDNQG VKFLRRFKQL
360 370 380 390 400
LNPAQVFDLM NGGPHLGLRL FQKFDNFRIL VCGGDGSVGW VLSEIDKLNL
410 420 430 440 450
NKQCQLGVLP LGTGNDLARV LGWGGSYDDD TQLPQILEKL ERASTKMLDR
460 470 480 490 500
WSIMTYELKL PAKASLLPEP PEASGGFYMT IYEDSVANHL TKILNSDEHA
510 520 530 540 550
VVISSAKILC ETVKDFVAKV EKAQDKTLEN TVVAEAVANK CSVLNEKLEQ
560 570 580 590 600
LLQALHADAQ ASRVPPGVGP AIPEEDAVES SSEESLGESK DQLVNDIAKP
610 620 630 640 650
SSQKAVKPRE IMLRANSLKK AVRQVIEEAG KVMDEQTVQP CEPVSPSCDY
660 670 680 690 700
DSPEADDSKD NDTKESPAAK STSQAPEAQA IRGHFQTDSV AGSAMATTKE
710 720 730 740 750
NLPVLNTRII CPGLRAGLAA SIAGSSIINK MLLANIDPFG ATPFIDPDLD
760 770 780 790 800
SLDGYSEKCV MNNYFGIGLD AKISLEFNNK REEHPEKCRS RTKNLMWYGV
810 820 830 840 850
LGTRELLQRS YKNLEQRVQL ECDGQYIPLP SLQGIAVLNI PSYAGGTNFW
860 870 880 890 900
GGTKEDDIFA APSFDDKILE VVAVFDSVQM AVSRVIKLQH HRIAQCRTVK
910 920 930 940 950
ITIFGDEGVP VQVDGEAWVQ PPGIIKIVHK NRAQMLTRDR AFESTLKSWE
960 970 980 990 1000
DKQKCDSGKP VLRTNLYIHP AADLATEEVS QMRLCSQAAE ELITRICDAA
1010 1020 1030 1040 1050
TIHCLLGAGT GSSVNACSHA LNKANPRFPE SLTRDTATEI AINVKALYNE
1060 1070 1080 1090 1100
TESLLVGRVP LQLESPHEER VSSALHSVEV ELQKLTEIPW LYYILRPNED
1110 1120 1130 1140 1150
EEPPMDCTKR NSKSTVFRIV PKFKMEKAQK QKTSSQPGPG DTESGSYEAN

SPGN
Length:1,154
Mass (Da):126,818
Last modified:November 1, 1996 - v1
Checksum:i0507DEECF64BC5CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U59429 mRNA. Translation: AAC52714.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U59429 mRNA. Translation: AAC52714.1 .

3D structure databases

ProteinModelPortali Q64398.
SMRi Q64398. Positions 222-286.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG051346.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. PE/DAG-bd.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view ]
Pfami PF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00169. PH. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00233. PH. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of a glucocorticoid-induced diacylglycerol kinase."
    Klauck T.M., Xu X., Mousseau B., Jaken S.
    J. Biol. Chem. 271:19781-19788(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Smooth muscle.

Entry informationi

Entry nameiDGKH_MESAU
AccessioniPrimary (citable) accession number: Q64398
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3