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Protein

Diacylglycerol kinase eta

Gene

DGKH

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization (By similarity).By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri169 – 219Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri241 – 292Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST52

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.107 3239

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase eta (EC:2.7.1.107)
Short name:
DAG kinase eta
Alternative name(s):
130 kDa diacylglycerol kinase
Diglyceride kinase eta
Short name:
DGK-eta
Gene namesi
Name:DGKH
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaCricetidaeCricetinaeMesocricetus
Proteomesi
  • UP000189706 Componenti: Genome assembly

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002184631 – 1154Diacylglycerol kinase etaAdd BLAST1154

Post-translational modificationi

Phosphorylated; does not inhibit catalytic activity.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Expressed in a wide variety of tissues. Most abundant in the brain and testis; also found in lung, spleen, and prostate.

Inductioni

By glucocorticoids.

Interactioni

Subunit structurei

Forms homooligomers through the SAM domain. Able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization inhibits its catalytic activity. Interacts with RAF1 and BRAF (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ64398
SMRiQ64398
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 152PHPROSITE-ProRule annotationAdd BLAST94
Domaini322 – 457DAGKcPROSITE-ProRule annotationAdd BLAST136

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri169 – 219Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri241 – 292Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST52

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG051346

Family and domain databases

CDDicd00029 C1, 2 hits
Gene3Di2.30.29.30, 1 hit
3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR037607 DGK
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00233 PH, 1 hit
SUPFAMiSSF111331 SSF111331, 2 hits
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q64398-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGAGYQHHP PGGAAVGTSA VSPTAAGPGE DSSDSEAEQG GPQKLIRKVS
60 70 80 90 100
TSGQIRTKTS IKEGQLLKQT SSFQRWKKRY FKLRGRTLYY AKDSKSLIFD
110 120 130 140 150
EVDLSDASVA EASTKNANNS FTIITPFRRL MLCAENRKEM EDWISSLKSV
160 170 180 190 200
QSREPYEVAQ FNVEHFSGMH NWYACSHARP TFCNVCRESL SGVTSHGLSC
210 220 230 240 250
EVCKFKAHKR CAVRATNNCK WTTLASIGKD IIEDEDGVAM PHQWLEGNLP
260 270 280 290 300
VSAKCAVCDK TCGSVLRLQD WKCLWCKAMV HTACKDLYHP VCPLGQCKVS
310 320 330 340 350
IIPPIALNST DSDGFCRATF SFCVSPLLVF VNSKSGDNQG VKFLRRFKQL
360 370 380 390 400
LNPAQVFDLM NGGPHLGLRL FQKFDNFRIL VCGGDGSVGW VLSEIDKLNL
410 420 430 440 450
NKQCQLGVLP LGTGNDLARV LGWGGSYDDD TQLPQILEKL ERASTKMLDR
460 470 480 490 500
WSIMTYELKL PAKASLLPEP PEASGGFYMT IYEDSVANHL TKILNSDEHA
510 520 530 540 550
VVISSAKILC ETVKDFVAKV EKAQDKTLEN TVVAEAVANK CSVLNEKLEQ
560 570 580 590 600
LLQALHADAQ ASRVPPGVGP AIPEEDAVES SSEESLGESK DQLVNDIAKP
610 620 630 640 650
SSQKAVKPRE IMLRANSLKK AVRQVIEEAG KVMDEQTVQP CEPVSPSCDY
660 670 680 690 700
DSPEADDSKD NDTKESPAAK STSQAPEAQA IRGHFQTDSV AGSAMATTKE
710 720 730 740 750
NLPVLNTRII CPGLRAGLAA SIAGSSIINK MLLANIDPFG ATPFIDPDLD
760 770 780 790 800
SLDGYSEKCV MNNYFGIGLD AKISLEFNNK REEHPEKCRS RTKNLMWYGV
810 820 830 840 850
LGTRELLQRS YKNLEQRVQL ECDGQYIPLP SLQGIAVLNI PSYAGGTNFW
860 870 880 890 900
GGTKEDDIFA APSFDDKILE VVAVFDSVQM AVSRVIKLQH HRIAQCRTVK
910 920 930 940 950
ITIFGDEGVP VQVDGEAWVQ PPGIIKIVHK NRAQMLTRDR AFESTLKSWE
960 970 980 990 1000
DKQKCDSGKP VLRTNLYIHP AADLATEEVS QMRLCSQAAE ELITRICDAA
1010 1020 1030 1040 1050
TIHCLLGAGT GSSVNACSHA LNKANPRFPE SLTRDTATEI AINVKALYNE
1060 1070 1080 1090 1100
TESLLVGRVP LQLESPHEER VSSALHSVEV ELQKLTEIPW LYYILRPNED
1110 1120 1130 1140 1150
EEPPMDCTKR NSKSTVFRIV PKFKMEKAQK QKTSSQPGPG DTESGSYEAN

SPGN
Length:1,154
Mass (Da):126,818
Last modified:November 1, 1996 - v1
Checksum:i0507DEECF64BC5CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59429 mRNA Translation: AAC52714.1

Similar proteinsi

Entry informationi

Entry nameiDGKH_MESAU
AccessioniPrimary (citable) accession number: Q64398
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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