Q64398 (DGKH_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase eta
Short name=DAG kinase eta
EC=2.7.1.107

Alternative name(s):
130 kDa diacylglycerol kinase
Diglyceride kinase eta
Short name=DGK-eta

Gene names

Name:

DGKH

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	1154 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization By similarity.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subunit structure	Forms homooligomers through the SAM domain. Able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization inhibits its catalytic activity. Interacts with RAF1 and BRAF By similarity.
Subcellular location	Cytoplasm. Cell membrane By similarity. Note: Translocated from the cytoplasm to endosomes in response to stress stimuli By similarity. Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS By similarity.
Tissue specificity	Expressed in a wide variety of tissues. Most abundant in the brain and testis; also found in lung, spleen, and prostate.
Induction	By glucocorticoids.
Post-translational modification	Phosphorylated; does not inhibit catalytic activity By similarity.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro intracellular signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1154	1154	Diacylglycerol kinase eta		PRO_0000218463
Regions
	Domain	59 – 152	94	PH
	Domain	322 – 457	136	DAGKc
	Zinc finger	169 – 219	51	Phorbol-ester/DAG-type 1
	Zinc finger	241 – 292	52	Phorbol-ester/DAG-type 2

Sequences

	Sequence		Length	Mass (Da)
	Q64398 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0507DEECF64BC5CB	FASTA	1,154	126,818
	10 20 30 40 50 60 MAGAGYQHHP PGGAAVGTSA VSPTAAGPGE DSSDSEAEQG GPQKLIRKVS TSGQIRTKTS 70 80 90 100 110 120 IKEGQLLKQT SSFQRWKKRY FKLRGRTLYY AKDSKSLIFD EVDLSDASVA EASTKNANNS 130 140 150 160 170 180 FTIITPFRRL MLCAENRKEM EDWISSLKSV QSREPYEVAQ FNVEHFSGMH NWYACSHARP 190 200 210 220 230 240 TFCNVCRESL SGVTSHGLSC EVCKFKAHKR CAVRATNNCK WTTLASIGKD IIEDEDGVAM 250 260 270 280 290 300 PHQWLEGNLP VSAKCAVCDK TCGSVLRLQD WKCLWCKAMV HTACKDLYHP VCPLGQCKVS 310 320 330 340 350 360 IIPPIALNST DSDGFCRATF SFCVSPLLVF VNSKSGDNQG VKFLRRFKQL LNPAQVFDLM 370 380 390 400 410 420 NGGPHLGLRL FQKFDNFRIL VCGGDGSVGW VLSEIDKLNL NKQCQLGVLP LGTGNDLARV 430 440 450 460 470 480 LGWGGSYDDD TQLPQILEKL ERASTKMLDR WSIMTYELKL PAKASLLPEP PEASGGFYMT 490 500 510 520 530 540 IYEDSVANHL TKILNSDEHA VVISSAKILC ETVKDFVAKV EKAQDKTLEN TVVAEAVANK 550 560 570 580 590 600 CSVLNEKLEQ LLQALHADAQ ASRVPPGVGP AIPEEDAVES SSEESLGESK DQLVNDIAKP 610 620 630 640 650 660 SSQKAVKPRE IMLRANSLKK AVRQVIEEAG KVMDEQTVQP CEPVSPSCDY DSPEADDSKD 670 680 690 700 710 720 NDTKESPAAK STSQAPEAQA IRGHFQTDSV AGSAMATTKE NLPVLNTRII CPGLRAGLAA 730 740 750 760 770 780 SIAGSSIINK MLLANIDPFG ATPFIDPDLD SLDGYSEKCV MNNYFGIGLD AKISLEFNNK 790 800 810 820 830 840 REEHPEKCRS RTKNLMWYGV LGTRELLQRS YKNLEQRVQL ECDGQYIPLP SLQGIAVLNI 850 860 870 880 890 900 PSYAGGTNFW GGTKEDDIFA APSFDDKILE VVAVFDSVQM AVSRVIKLQH HRIAQCRTVK 910 920 930 940 950 960 ITIFGDEGVP VQVDGEAWVQ PPGIIKIVHK NRAQMLTRDR AFESTLKSWE DKQKCDSGKP 970 980 990 1000 1010 1020 VLRTNLYIHP AADLATEEVS QMRLCSQAAE ELITRICDAA TIHCLLGAGT GSSVNACSHA 1030 1040 1050 1060 1070 1080 LNKANPRFPE SLTRDTATEI AINVKALYNE TESLLVGRVP LQLESPHEER VSSALHSVEV 1090 1100 1110 1120 1130 1140 ELQKLTEIPW LYYILRPNED EEPPMDCTKR NSKSTVFRIV PKFKMEKAQK QKTSSQPGPG 1150 DTESGSYEAN SPGN

References

[1]	"Cloning and characterization of a glucocorticoid-induced diacylglycerol kinase." Klauck T.M., Xu X., Mousseau B., Jaken S. J. Biol. Chem. 271:19781-19788(1996) [PubMed: 8702685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Smooth muscle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U59429 mRNA. Translation: AAC52714.1.
3D structure databases
ProteinModelPortal	Q64398.
SMR	Q64398. Positions 222-286.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG051346.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGKH_MESAU

Accession

Primary (citable) accession number: Q64398

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program