SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q64398

- DGKH_MESAU

UniProt

Q64398 - DGKH_MESAU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Diacylglycerol kinase eta
Gene
DGKH
Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization By similarity.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri169 – 21951Phorbol-ester/DAG-type 1
Add
BLAST
Zinc fingeri241 – 29252Phorbol-ester/DAG-type 2
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: InterPro
  3. diacylglycerol kinase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase eta (EC:2.7.1.107)
Short name:
DAG kinase eta
Alternative name(s):
130 kDa diacylglycerol kinase
Diglyceride kinase eta
Short name:
DGK-eta
Gene namesi
Name:DGKH
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

Cytoplasm. Cell membrane By similarity
Note: Translocated from the cytoplasm to endosomes in response to stress stimuli By similarity. Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11541154Diacylglycerol kinase eta
PRO_0000218463Add
BLAST

Post-translational modificationi

Phosphorylated; does not inhibit catalytic activity By similarity.

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Expressed in a wide variety of tissues. Most abundant in the brain and testis; also found in lung, spleen, and prostate.

Inductioni

By glucocorticoids.

Interactioni

Subunit structurei

Forms homooligomers through the SAM domain. Able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization inhibits its catalytic activity. Interacts with RAF1 and BRAF By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ64398.
SMRiQ64398. Positions 222-286.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 15294PH
Add
BLAST
Domaini322 – 457136DAGKc
Add
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.
Contains 1 PH domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG051346.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64398-1 [UniParc]FASTAAdd to Basket

« Hide

MAGAGYQHHP PGGAAVGTSA VSPTAAGPGE DSSDSEAEQG GPQKLIRKVS     50
TSGQIRTKTS IKEGQLLKQT SSFQRWKKRY FKLRGRTLYY AKDSKSLIFD 100
EVDLSDASVA EASTKNANNS FTIITPFRRL MLCAENRKEM EDWISSLKSV 150
QSREPYEVAQ FNVEHFSGMH NWYACSHARP TFCNVCRESL SGVTSHGLSC 200
EVCKFKAHKR CAVRATNNCK WTTLASIGKD IIEDEDGVAM PHQWLEGNLP 250
VSAKCAVCDK TCGSVLRLQD WKCLWCKAMV HTACKDLYHP VCPLGQCKVS 300
IIPPIALNST DSDGFCRATF SFCVSPLLVF VNSKSGDNQG VKFLRRFKQL 350
LNPAQVFDLM NGGPHLGLRL FQKFDNFRIL VCGGDGSVGW VLSEIDKLNL 400
NKQCQLGVLP LGTGNDLARV LGWGGSYDDD TQLPQILEKL ERASTKMLDR 450
WSIMTYELKL PAKASLLPEP PEASGGFYMT IYEDSVANHL TKILNSDEHA 500
VVISSAKILC ETVKDFVAKV EKAQDKTLEN TVVAEAVANK CSVLNEKLEQ 550
LLQALHADAQ ASRVPPGVGP AIPEEDAVES SSEESLGESK DQLVNDIAKP 600
SSQKAVKPRE IMLRANSLKK AVRQVIEEAG KVMDEQTVQP CEPVSPSCDY 650
DSPEADDSKD NDTKESPAAK STSQAPEAQA IRGHFQTDSV AGSAMATTKE 700
NLPVLNTRII CPGLRAGLAA SIAGSSIINK MLLANIDPFG ATPFIDPDLD 750
SLDGYSEKCV MNNYFGIGLD AKISLEFNNK REEHPEKCRS RTKNLMWYGV 800
LGTRELLQRS YKNLEQRVQL ECDGQYIPLP SLQGIAVLNI PSYAGGTNFW 850
GGTKEDDIFA APSFDDKILE VVAVFDSVQM AVSRVIKLQH HRIAQCRTVK 900
ITIFGDEGVP VQVDGEAWVQ PPGIIKIVHK NRAQMLTRDR AFESTLKSWE 950
DKQKCDSGKP VLRTNLYIHP AADLATEEVS QMRLCSQAAE ELITRICDAA 1000
TIHCLLGAGT GSSVNACSHA LNKANPRFPE SLTRDTATEI AINVKALYNE 1050
TESLLVGRVP LQLESPHEER VSSALHSVEV ELQKLTEIPW LYYILRPNED 1100
EEPPMDCTKR NSKSTVFRIV PKFKMEKAQK QKTSSQPGPG DTESGSYEAN 1150
SPGN 1154
Length:1,154
Mass (Da):126,818
Last modified:November 1, 1996 - v1
Checksum:i0507DEECF64BC5CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U59429 mRNA. Translation: AAC52714.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U59429 mRNA. Translation: AAC52714.1 .

3D structure databases

ProteinModelPortali Q64398.
SMRi Q64398. Positions 222-286.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG051346.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view ]
Pfami PF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00169. PH. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00233. PH. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of a glucocorticoid-induced diacylglycerol kinase."
    Klauck T.M., Xu X., Mousseau B., Jaken S.
    J. Biol. Chem. 271:19781-19788(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Smooth muscle.

Entry informationi

Entry nameiDGKH_MESAU
AccessioniPrimary (citable) accession number: Q64398
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi