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Q64393

- NEUR2_CRIGR

UniProt

Q64393 - NEUR2_CRIGR

Protein

Sialidase-2

Gene

NEU2

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins, oligosaccharides and gangliosides.1 Publication

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei21 – 211SubstrateBy similarity
    Binding sitei41 – 411SubstrateBy similarity
    Active sitei46 – 461Proton acceptorBy similarity
    Binding sitei179 – 1791SubstrateBy similarity
    Binding sitei181 – 1811SubstrateBy similarity
    Binding sitei218 – 2181SubstrateBy similarity
    Binding sitei237 – 2371SubstrateSequence Analysis
    Binding sitei303 – 3031SubstrateBy similarity
    Active sitei333 – 3331NucleophileBy similarity
    Active sitei354 – 3541Sequence Analysis

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. ganglioside catabolic process Source: UniProtKB
    2. oligosaccharide catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Lipid degradation, Lipid metabolism

    Protein family/group databases

    CAZyiGH33. Glycoside Hydrolase Family 33.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sialidase-2 (EC:3.2.1.18)
    Alternative name(s):
    Cytosolic sialidase
    N-acetyl-alpha-neuraminidase 2
    Gene namesi
    Name:NEU2
    OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
    Taxonomic identifieri10029 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 379379Sialidase-2PRO_0000208898Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ64393.
    SMRiQ64393. Positions 1-376.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati127 – 13812BNR 1Add
    BLAST
    Repeati197 – 20812BNR 2Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi20 – 234FRIP motif

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 33 family.Curated
    Contains 2 BNR repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG052608.
    KOiK12357.

    Family and domain databases

    Gene3Di2.120.10.10. 1 hit.
    InterProiIPR026945. Sialidase-2.
    IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view]
    PANTHERiPTHR10628. PTHR10628. 1 hit.
    PTHR10628:SF6. PTHR10628:SF6. 1 hit.
    SUPFAMiSSF50939. SSF50939. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q64393-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATCPVLQKE TLFQTGDYAY RIPALIYLSK QKTLLAFAEK RLTKTDEHAD    50
    LFVLRRGSYN ADTHQVQWQA EEVVTQAYLE GHRSMSPCPL YDKQTRTLFL 100
    FFIAVRGQIS EHHQLQTGVN VTRLCHITST DHGKTWSAVQ DLTDTTIGST 150
    HQDWATFGVG PGHCLQLRNT AGSLLVPAYA YRKQPPIHAP APSAFCFLSH 200
    DHGSTWELGH FVSQNSLECQ VAEVGTGAER VVYLNARSCL GARVQAQSPN 250
    SGLDFQDNQV VSKLVEPPKG CHGSVIAFPN PTSKADALDV WLLYTHPTDS 300
    RKRTNLGVYL NQKPLDPTTW SAPTLLATGI CAYSDLQNMG HGPDGSPQFG 350
    CLYESNNYEE IVFLMFTLKQ AFPAVFGAQ 379
    Length:379
    Mass (Da):41,962
    Last modified:November 1, 1996 - v1
    Checksum:iB5AFFBC6B6BE88B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U06143 mRNA. Translation: AAA19746.1.
    PIRiA54961.
    RefSeqiNP_001233664.1. NM_001246735.2.

    Genome annotation databases

    GeneIDi100689301.
    KEGGicge:100689301.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U06143 mRNA. Translation: AAA19746.1 .
    PIRi A54961.
    RefSeqi NP_001233664.1. NM_001246735.2.

    3D structure databases

    ProteinModelPortali Q64393.
    SMRi Q64393. Positions 1-376.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH33. Glycoside Hydrolase Family 33.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100689301.
    KEGGi cge:100689301.

    Organism-specific databases

    CTDi 4759.

    Phylogenomic databases

    HOVERGENi HBG052608.
    KOi K12357.

    Family and domain databases

    Gene3Di 2.120.10.10. 1 hit.
    InterProi IPR026945. Sialidase-2.
    IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view ]
    PANTHERi PTHR10628. PTHR10628. 1 hit.
    PTHR10628:SF6. PTHR10628:SF6. 1 hit.
    SUPFAMi SSF50939. SSF50939. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a soluble sialidase from Chinese hamster ovary cells: sequence alignment similarities to bacterial sialidases."
      Ferrari J., Harris R., Warner T.G.
      Glycobiology 4:367-373(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ovary.
    2. "Characterization of cytosolic sialidase from Chinese hamster ovary cells: part I: cloning and expression of soluble sialidase in Escherichia coli."
      Burg M., Muthing J.
      Carbohydr. Res. 330:335-346(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY.
    3. "Characterization of cytosolic sialidase from Chinese hamster ovary cells: part II. Substrate specificity for gangliosides."
      Muthing J., Burg M.
      Carbohydr. Res. 330:347-356(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiNEUR2_CRIGR
    AccessioniPrimary (citable) accession number: Q64393
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3