Reviewed, UniProtKB/Swiss-Prot Q64393 (NEUR2_CRIGR)

Names and origin

Protein names

Recommended name:
    Sialidase-2
    EC=3.2.1.18
Alternative name(s):
    Cytosolic sialidase
    N-acetyl-alpha-neuraminidase 2

Gene names

Name:

NEU2

Organism

Cricetulus griseus (Chinese hamster)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes sialylated compounds.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 33 family. Contains 2 BNR repeats.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Sialidase-2		PRO_0000208898
Regions
	Repeat	127 – 138	12	BNR 1
	Repeat	197 – 208	12	BNR 2
	Motif	20 – 23	4	FRIP motif
Sites
	Active site	46	1	Proton acceptor By similarity
	Active site	333	1	Nucleophile By similarity
	Active site	354	1	Potential
	Binding site	21	1	Substrate By similarity
	Binding site	237	1	Substrate Potential
	Binding site	303	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64393-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B5AFFBC6B6BE88B1	FASTA	379	41,962
	10 20 30 40 50 60 MATCPVLQKE TLFQTGDYAY RIPALIYLSK QKTLLAFAEK RLTKTDEHAD LFVLRRGSYN 70 80 90 100 110 120 ADTHQVQWQA EEVVTQAYLE GHRSMSPCPL YDKQTRTLFL FFIAVRGQIS EHHQLQTGVN 130 140 150 160 170 180 VTRLCHITST DHGKTWSAVQ DLTDTTIGST HQDWATFGVG PGHCLQLRNT AGSLLVPAYA 190 200 210 220 230 240 YRKQPPIHAP APSAFCFLSH DHGSTWELGH FVSQNSLECQ VAEVGTGAER VVYLNARSCL 250 260 270 280 290 300 GARVQAQSPN SGLDFQDNQV VSKLVEPPKG CHGSVIAFPN PTSKADALDV WLLYTHPTDS 310 320 330 340 350 360 RKRTNLGVYL NQKPLDPTTW SAPTLLATGI CAYSDLQNMG HGPDGSPQFG CLYESNNYEE 370 IVFLMFTLKQ AFPAVFGAQ

References

[1]	"Cloning and expression of a soluble sialidase from Chinese hamster ovary cells: sequence alignment similarities to bacterial sialidases." Ferrari J., Harris R., Warner T.G. Glycobiology 4:367-373(1994) [PubMed: 7949662] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U06143 mRNA. Translation: AAA19746.1.
PIR	A54961.
3D structure databases
SMR	Q64393. Positions 1-376.
ModBase	Search...
Protein family/group databases
CAZy	GH33. Glycoside Hydrolase Family 33.
Phylogenomic databases
HOVERGEN	Q64393.
Enzyme and pathway databases
BRENDA	3.2.1.18. 18.
Family and domain databases
InterPro	IPR011040. Neuraminidase. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NEUR2_CRIGR

Accession

Primary (citable) accession number: Q64393

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)