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Q64392 (AT12A_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Potassium-transporting ATPase alpha chain 2
EC=3.6.3.10
Alternative name(s):
Non-gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene names
Name:ATP12A
Synonyms:ATP1AL1
OrganismCavia porcellus (Guinea pig)
Taxonomic identifier10141 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length1033 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Catalytic activity

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Subunit structure

Composed of two subunits: alpha (catalytic) and beta.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Found in skin, kidney and distal colon. Ref.2

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10331033Potassium-transporting ATPase alpha chain 2
PRO_0000046259

Regions

Topological domain1 – 9696Cytoplasmic Potential
Transmembrane97 – 11721Helical; Potential
Topological domain118 – 14023Lumenal Potential
Transmembrane141 – 16121Helical; Potential
Topological domain162 – 297136Cytoplasmic Potential
Transmembrane298 – 31720Helical; Potential
Topological domain318 – 32912Lumenal Potential
Transmembrane330 – 34718Helical; Potential
Topological domain348 – 781434Cytoplasmic Potential
Transmembrane782 – 80120Helical; Potential
Topological domain802 – 81110Lumenal Potential
Transmembrane812 – 83221Helical; Potential
Topological domain833 – 85220Cytoplasmic Potential
Transmembrane853 – 87523Helical; Potential
Topological domain876 – 92752Lumenal Potential
Transmembrane928 – 94720Helical; Potential
Topological domain948 – 96114Cytoplasmic Potential
Transmembrane962 – 98019Helical; Potential
Topological domain981 – 99515Lumenal Potential
Transmembrane996 – 101621Helical; Potential
Topological domain1017 – 103317Cytoplasmic Potential

Sites

Active site38514-aspartylphosphate intermediate By similarity
Metal binding7261Magnesium By similarity
Metal binding7301Magnesium By similarity

Amino acid modifications

Modified residue9521Phosphoserine; by PKA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64392 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C4EEE72D83C1F40C

FASTA1,033114,557
        10         20         30         40         50         60 
MRRKTLEIYS VELDGTKDTK QLGQEEGKKC NELDLKKSSQ KEELKKELDL DDHKLTSEEL 

        70         80         90        100        110        120 
EQKYGTNIIR GLSSTRAAEL LARDGPNALS PPKQTPEIIK FLKQMIGGFS ILLWVGAILC 

       130        140        150        160        170        180 
WIAYGIQYAS NQSGSLDNVY LGVVLALVVI LTGIFAYYQE AKSTNIMSSF SKMIPQEALV 

       190        200        210        220        230        240 
TRDAEKKVIP AEQLVVGDIV EIKGGDQIPA DIRLLFSQGC KVDNSSLTGE SEPQPRSAEF 

       250        260        270        280        290        300 
THENPLETKN IAFYSTTCLE GTATGMVINT GDRTIIGRIA SLASGVGNEK TPIATEIEHF 

       310        320        330        340        350        360 
VHIVAGVAVS IGILFFIIAV SLKYRVLDSI IFLIGIIVAN VPEGLLATVT VTLSLTAKRM 

       370        380        390        400        410        420 
AKKNCLVKNL EAVETLGSTS VICSDKTGTL TQNRMTVAHL WFDSQIFTAD TSESQSNQAF 

       430        440        450        460        470        480 
DQSSGTWASL SKIIALCNRA EFRPGQENVP IMKRVVVGDA SETALLKFSE VILGDVMEIR 

       490        500        510        520        530        540 
KRNRKVAEIP FNSTNKFQLS IHETEDPGDP RFLMVMKGAP ERILEKCSTI MINGQEQPLD 

       550        560        570        580        590        600 
KNNANAFHTA YMELGGMGER VLGFCHLYLP AHEFPENYSF DVDTMNFPTS NLCFVGLLSM 

       610        620        630        640        650        660 
IDPPRSTVPD AVAKCRSAGI KVIMVTGDHP ITAKAIAKSV GIISANSETV EDIAKRCNIA 

       670        680        690        700        710        720 
VEQVNKQDAR AAVVTGMELK DMTPEQLDEI LANYPEIVFA RTSPQQKLII VEGCQRQNAV 

       730        740        750        760        770        780 
VAVTGDGVND SPALKKADIG IAMGIAGSDA AKNAADMVLL DDNFASIVTG VEEGRLIFDN 

       790        800        810        820        830        840 
LKKTIAYTLT KNIAELCPFL VYIIVGLPLP IGTITILFID LGTDIIPSIA LAYEKVESDI 

       850        860        870        880        890        900 
MNRKPRHKKK DRLVNHQLAI YSYLHIGLMQ ALGAFLVYFT VYAQQGFWPT SLIQLRVKWE 

       910        920        930        940        950        960 
QDYVNDLEDS YGQQWTRYQR KYLEWTGYTA FFVGIMVQQI ADLIIRKTRR NSIFQQGLFR 

       970        980        990       1000       1010       1020 
NKVIWVGITS QIIVALILSC GLGSITALNF TMLRVQYWFV AVPHAILIWV YDEVRKLFLR 

      1030 
LYPGSWWDKN MYY

« Hide

References

[1]"Isolation and characterization of cDNA encoding the putative guinea pig distal colon H+, K+ -ATPase alpha subunit."
Watanabe T., Sato M., Kaneko K., Suzuki T., Yoshida T., Suzuki Y.
Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Hartley.
[2]"Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit."
Pestov N.B., Romanova L.G., Korneenko T.V., Egorov M.V., Kostina M.B., Sverdlov V.E., Askari A., Shakhparonov M.I., Modyanov N.N.
FEBS Lett. 440:320-324(1998) [PubMed: 9872395] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D21854 mRNA. Translation: BAA04880.1.
RefSeqNP_001166387.1. NM_001172916.1.

3D structure databases

ProteinModelPortalQ64392.
SMRQ64392. Positions 36-1033.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSCPOT00000003451; ENSCPOP00000003081; ENSCPOG00000003407.
GeneID100135482.

Organism-specific databases

CTD479.

Phylogenomic databases

eggNOGroNOG10939.
GeneTreeENSGT00560000076866.
HOVERGENHBG004298.
InParanoidQ64392.
OMAALVIRDS.
OrthoDBEOG41C6VF.

Family and domain databases

InterProIPR023306. ATPase_cation_domN.
IPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR005775. ATPase_P-typ_cation-ex_asu_euk.
IPR006069. ATPase_P-typ_cation-exchng_asu.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
[Graphical view]
Gene3DG3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 1 hit.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit.
G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 2 hits.
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00121. NAKATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF81660. ATPase_cation_domN. 1 hit.
SSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAT12A_CAVPO
AccessionPrimary (citable) accession number: Q64392
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: September 21, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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