Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium-transporting ATPase alpha chain 2

Gene

ATP12A

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3854-aspartylphosphate intermediateBy similarity1
Metal bindingi726MagnesiumBy similarity1
Metal bindingi730MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 2 (EC:3.6.3.10)
Alternative name(s):
Non-gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:ATP12A
Synonyms:ATP1AL1
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 96CytoplasmicSequence analysisAdd BLAST95
Transmembranei97 – 117HelicalSequence analysisAdd BLAST21
Topological domaini118 – 140LumenalSequence analysisAdd BLAST23
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 297CytoplasmicSequence analysisAdd BLAST136
Transmembranei298 – 317HelicalSequence analysisAdd BLAST20
Topological domaini318 – 329LumenalSequence analysisAdd BLAST12
Transmembranei330 – 347HelicalSequence analysisAdd BLAST18
Topological domaini348 – 781CytoplasmicSequence analysisAdd BLAST434
Transmembranei782 – 801HelicalSequence analysisAdd BLAST20
Topological domaini802 – 811LumenalSequence analysis10
Transmembranei812 – 832HelicalSequence analysisAdd BLAST21
Topological domaini833 – 852CytoplasmicSequence analysisAdd BLAST20
Transmembranei853 – 875HelicalSequence analysisAdd BLAST23
Topological domaini876 – 927LumenalSequence analysisAdd BLAST52
Transmembranei928 – 947HelicalSequence analysisAdd BLAST20
Topological domaini948 – 961CytoplasmicSequence analysisAdd BLAST14
Transmembranei962 – 980HelicalSequence analysisAdd BLAST19
Topological domaini981 – 995LumenalSequence analysisAdd BLAST15
Transmembranei996 – 1016HelicalSequence analysisAdd BLAST21
Topological domaini1017 – 1033CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000462592 – 1033Potassium-transporting ATPase alpha chain 2Add BLAST1032

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphotyrosineBy similarity1
Modified residuei10PhosphoserineBy similarity1
Modified residuei237PhosphoserineBy similarity1
Modified residuei284PhosphoserineBy similarity1
Modified residuei461PhosphoserineBy similarity1
Modified residuei599PhosphoserineBy similarity1
Modified residuei838PhosphoserineBy similarity1
Modified residuei952Phosphoserine; by PKABy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ64392.

Expressioni

Tissue specificityi

Found in skin, kidney and distal colon.1 Publication

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000003081.

Structurei

3D structure databases

ProteinModelPortaliQ64392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00850000132256.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ64392.
OMAiNQVFDQS.
OrthoDBiEOG091G01BB.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030318. Atp12a.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF282. PTHR24093:SF282. 2 hits.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64392-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRKTLEIYS VELDGTKDTK QLGQEEGKKC NELDLKKSSQ KEELKKELDL
60 70 80 90 100
DDHKLTSEEL EQKYGTNIIR GLSSTRAAEL LARDGPNALS PPKQTPEIIK
110 120 130 140 150
FLKQMIGGFS ILLWVGAILC WIAYGIQYAS NQSGSLDNVY LGVVLALVVI
160 170 180 190 200
LTGIFAYYQE AKSTNIMSSF SKMIPQEALV TRDAEKKVIP AEQLVVGDIV
210 220 230 240 250
EIKGGDQIPA DIRLLFSQGC KVDNSSLTGE SEPQPRSAEF THENPLETKN
260 270 280 290 300
IAFYSTTCLE GTATGMVINT GDRTIIGRIA SLASGVGNEK TPIATEIEHF
310 320 330 340 350
VHIVAGVAVS IGILFFIIAV SLKYRVLDSI IFLIGIIVAN VPEGLLATVT
360 370 380 390 400
VTLSLTAKRM AKKNCLVKNL EAVETLGSTS VICSDKTGTL TQNRMTVAHL
410 420 430 440 450
WFDSQIFTAD TSESQSNQAF DQSSGTWASL SKIIALCNRA EFRPGQENVP
460 470 480 490 500
IMKRVVVGDA SETALLKFSE VILGDVMEIR KRNRKVAEIP FNSTNKFQLS
510 520 530 540 550
IHETEDPGDP RFLMVMKGAP ERILEKCSTI MINGQEQPLD KNNANAFHTA
560 570 580 590 600
YMELGGMGER VLGFCHLYLP AHEFPENYSF DVDTMNFPTS NLCFVGLLSM
610 620 630 640 650
IDPPRSTVPD AVAKCRSAGI KVIMVTGDHP ITAKAIAKSV GIISANSETV
660 670 680 690 700
EDIAKRCNIA VEQVNKQDAR AAVVTGMELK DMTPEQLDEI LANYPEIVFA
710 720 730 740 750
RTSPQQKLII VEGCQRQNAV VAVTGDGVND SPALKKADIG IAMGIAGSDA
760 770 780 790 800
AKNAADMVLL DDNFASIVTG VEEGRLIFDN LKKTIAYTLT KNIAELCPFL
810 820 830 840 850
VYIIVGLPLP IGTITILFID LGTDIIPSIA LAYEKVESDI MNRKPRHKKK
860 870 880 890 900
DRLVNHQLAI YSYLHIGLMQ ALGAFLVYFT VYAQQGFWPT SLIQLRVKWE
910 920 930 940 950
QDYVNDLEDS YGQQWTRYQR KYLEWTGYTA FFVGIMVQQI ADLIIRKTRR
960 970 980 990 1000
NSIFQQGLFR NKVIWVGITS QIIVALILSC GLGSITALNF TMLRVQYWFV
1010 1020 1030
AVPHAILIWV YDEVRKLFLR LYPGSWWDKN MYY
Length:1,033
Mass (Da):114,557
Last modified:November 1, 1996 - v1
Checksum:iC4EEE72D83C1F40C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21854 mRNA. Translation: BAA04880.1.
RefSeqiNP_001166387.1. NM_001172916.1.

Genome annotation databases

EnsembliENSCPOT00000003451; ENSCPOP00000003081; ENSCPOG00000003407.
GeneIDi100135482.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21854 mRNA. Translation: BAA04880.1.
RefSeqiNP_001166387.1. NM_001172916.1.

3D structure databases

ProteinModelPortaliQ64392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000003081.

Proteomic databases

PRIDEiQ64392.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCPOT00000003451; ENSCPOP00000003081; ENSCPOG00000003407.
GeneIDi100135482.

Organism-specific databases

CTDi479.

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00850000132256.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ64392.
OMAiNQVFDQS.
OrthoDBiEOG091G01BB.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030318. Atp12a.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF282. PTHR24093:SF282. 2 hits.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT12A_CAVPO
AccessioniPrimary (citable) accession number: Q64392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.