Reviewed, UniProtKB/Swiss-Prot Q64392 (AT12A_CAVPO)

Names and origin

Protein names

Recommended name:
    Potassium-transporting ATPase alpha chain 2
    EC=3.6.3.10
Alternative name(s):
    Proton pump
    Non-gastric H(+)/K(+) ATPase subunit alpha

Gene names

Name:	ATP12A
Synonyms:	ATP1AL1

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	1033 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.
Catalytic activity	ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).
Subunit structure	Composed of two subunits: alpha (catalytic) and beta.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Found in skin, kidney and distal colon. Ref.2
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro potassium ion transport Inferred from electronic annotation. Source: UniProtKB-KW proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrogen:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1033	1033	Potassium-transporting ATPase alpha chain 2		PRO_0000046259
Regions
	Topological domain	1 – 96	96	Cytoplasmic Potential
	Transmembrane	97 – 117	21	Potential
	Topological domain	118 – 140	23	Lumenal Potential
	Transmembrane	141 – 161	21	Potential
	Topological domain	162 – 297	136	Cytoplasmic Potential
	Transmembrane	298 – 317	20	Potential
	Topological domain	318 – 329	12	Lumenal Potential
	Transmembrane	330 – 347	18	Potential
	Topological domain	348 – 781	434	Cytoplasmic Potential
	Transmembrane	782 – 801	20	Potential
	Topological domain	802 – 811	10	Lumenal Potential
	Transmembrane	812 – 832	21	Potential
	Topological domain	833 – 852	20	Cytoplasmic Potential
	Transmembrane	853 – 875	23	Potential
	Topological domain	876 – 927	52	Lumenal Potential
	Transmembrane	928 – 947	20	Potential
	Topological domain	948 – 961	14	Cytoplasmic Potential
	Transmembrane	962 – 980	19	Potential
	Topological domain	981 – 995	15	Lumenal Potential
	Transmembrane	996 – 1016	21	Potential
	Topological domain	1017 – 1033	17	Cytoplasmic Potential
Sites
	Active site	385	1	4-aspartylphosphate intermediate By similarity
	Metal binding	726	1	Magnesium By similarity
	Metal binding	730	1	Magnesium By similarity
Amino acid modifications
	Modified residue	952	1	Phosphoserine; by PKA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64392-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C4EEE72D83C1F40C	FASTA	1,033	114,557
	10 20 30 40 50 60 MRRKTLEIYS VELDGTKDTK QLGQEEGKKC NELDLKKSSQ KEELKKELDL DDHKLTSEEL 70 80 90 100 110 120 EQKYGTNIIR GLSSTRAAEL LARDGPNALS PPKQTPEIIK FLKQMIGGFS ILLWVGAILC 130 140 150 160 170 180 WIAYGIQYAS NQSGSLDNVY LGVVLALVVI LTGIFAYYQE AKSTNIMSSF SKMIPQEALV 190 200 210 220 230 240 TRDAEKKVIP AEQLVVGDIV EIKGGDQIPA DIRLLFSQGC KVDNSSLTGE SEPQPRSAEF 250 260 270 280 290 300 THENPLETKN IAFYSTTCLE GTATGMVINT GDRTIIGRIA SLASGVGNEK TPIATEIEHF 310 320 330 340 350 360 VHIVAGVAVS IGILFFIIAV SLKYRVLDSI IFLIGIIVAN VPEGLLATVT VTLSLTAKRM 370 380 390 400 410 420 AKKNCLVKNL EAVETLGSTS VICSDKTGTL TQNRMTVAHL WFDSQIFTAD TSESQSNQAF 430 440 450 460 470 480 DQSSGTWASL SKIIALCNRA EFRPGQENVP IMKRVVVGDA SETALLKFSE VILGDVMEIR 490 500 510 520 530 540 KRNRKVAEIP FNSTNKFQLS IHETEDPGDP RFLMVMKGAP ERILEKCSTI MINGQEQPLD 550 560 570 580 590 600 KNNANAFHTA YMELGGMGER VLGFCHLYLP AHEFPENYSF DVDTMNFPTS NLCFVGLLSM 610 620 630 640 650 660 IDPPRSTVPD AVAKCRSAGI KVIMVTGDHP ITAKAIAKSV GIISANSETV EDIAKRCNIA 670 680 690 700 710 720 VEQVNKQDAR AAVVTGMELK DMTPEQLDEI LANYPEIVFA RTSPQQKLII VEGCQRQNAV 730 740 750 760 770 780 VAVTGDGVND SPALKKADIG IAMGIAGSDA AKNAADMVLL DDNFASIVTG VEEGRLIFDN 790 800 810 820 830 840 LKKTIAYTLT KNIAELCPFL VYIIVGLPLP IGTITILFID LGTDIIPSIA LAYEKVESDI 850 860 870 880 890 900 MNRKPRHKKK DRLVNHQLAI YSYLHIGLMQ ALGAFLVYFT VYAQQGFWPT SLIQLRVKWE 910 920 930 940 950 960 QDYVNDLEDS YGQQWTRYQR KYLEWTGYTA FFVGIMVQQI ADLIIRKTRR NSIFQQGLFR 970 980 990 1000 1010 1020 NKVIWVGITS QIIVALILSC GLGSITALNF TMLRVQYWFV AVPHAILIWV YDEVRKLFLR 1030 LYPGSWWDKN MYY

References

[1]	"Isolation and characterization of cDNA encoding the putative guinea pig distal colon H+, K+ -ATPase alpha subunit." Watanabe T., Sato M., Kaneko K., Suzuki T., Yoshida T., Suzuki Y. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley.
[2]	"Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit." Pestov N.B., Romanova L.G., Korneenko T.V., Egorov M.V., Kostina M.B., Sverdlov V.E., Askari A., Shakhparonov M.I., Modyanov N.N. FEBS Lett. 440:320-324(1998) [PubMed: 9872395] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D21854 mRNA. Translation: BAA04880.1.
3D structure databases
SMR	Q64392. Positions 36-1033.
ModBase	Search...
Protein-protein interaction databases
STRING	Q64392.
Genome annotation databases
Ensembl	ENSCPOT00000003451; ENSCPOP00000003081; ENSCPOG00000003407; Cavia porcellus. [Genome view]
Phylogenomic databases
eggNOG	roNOG10939.
HOVERGEN	Q64392.
InParanoid	Q64392.
OMA	HTAYMEL.
OrthoDB	EOG980MG3.
Enzyme and pathway databases
BRENDA	3.6.3.10. 44.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR005775. ATPase_P-typ_cation-ex_asu_euk. IPR006069. ATPase_P-typ_cation-exchng_asu. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00121. NAKATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01106. ATPase-IIC_X-K. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AT12A_CAVPO

Accession

Primary (citable) accession number: Q64392

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)