Q64392 (AT12A_CAVPO) Reviewed, UniProtKB/Swiss-Prot
Last modified
September 21, 2011.
Version 94.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Potassium-transporting ATPase alpha chain 2 EC=3.6.3.10 Alternative name(s): Non-gastric H(+)/K(+) ATPase subunit alpha Proton pump | ||||
| Gene names |
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| Organism | Cavia porcellus (Guinea pig) | ||||
| Taxonomic identifier | 10141 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia |
Protein attributes
| Sequence length | 1033 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues. |
| Catalytic activity | ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In). |
| Subunit structure | Composed of two subunits: alpha (catalytic) and beta. |
| Subcellular location | |
| Tissue specificity | Found in skin, kidney and distal colon. Ref.2 |
| Sequence similarities | Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. [View classification] |
Ontologies
| Keywords | |
|---|---|
| Biological process | Hydrogen ion transport Ion transport Potassium transport Transport |
| Cellular component | Membrane |
| Domain | Transmembrane Transmembrane helix |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium |
| Molecular function | Hydrolase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | ATP biosynthetic process Inferred from electronic annotation. Source: InterPro |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrogen:potassium-exchanging ATPase activityInferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1033 | 1033 | Potassium-transporting ATPase alpha chain 2 | PRO_0000046259 | |||||
Regions | |||||||||
| Topological domain | 1 – 96 | 96 | Cytoplasmic Potential | ||||||
| Transmembrane | 97 – 117 | 21 | Helical; Potential | ||||||
| Topological domain | 118 – 140 | 23 | Lumenal Potential | ||||||
| Transmembrane | 141 – 161 | 21 | Helical; Potential | ||||||
| Topological domain | 162 – 297 | 136 | Cytoplasmic Potential | ||||||
| Transmembrane | 298 – 317 | 20 | Helical; Potential | ||||||
| Topological domain | 318 – 329 | 12 | Lumenal Potential | ||||||
| Transmembrane | 330 – 347 | 18 | Helical; Potential | ||||||
| Topological domain | 348 – 781 | 434 | Cytoplasmic Potential | ||||||
| Transmembrane | 782 – 801 | 20 | Helical; Potential | ||||||
| Topological domain | 802 – 811 | 10 | Lumenal Potential | ||||||
| Transmembrane | 812 – 832 | 21 | Helical; Potential | ||||||
| Topological domain | 833 – 852 | 20 | Cytoplasmic Potential | ||||||
| Transmembrane | 853 – 875 | 23 | Helical; Potential | ||||||
| Topological domain | 876 – 927 | 52 | Lumenal Potential | ||||||
| Transmembrane | 928 – 947 | 20 | Helical; Potential | ||||||
| Topological domain | 948 – 961 | 14 | Cytoplasmic Potential | ||||||
| Transmembrane | 962 – 980 | 19 | Helical; Potential | ||||||
| Topological domain | 981 – 995 | 15 | Lumenal Potential | ||||||
| Transmembrane | 996 – 1016 | 21 | Helical; Potential | ||||||
| Topological domain | 1017 – 1033 | 17 | Cytoplasmic Potential | ||||||
Sites | |||||||||
| Active site | 385 | 1 | 4-aspartylphosphate intermediate By similarity | ||||||
| Metal binding | 726 | 1 | Magnesium By similarity | ||||||
| Metal binding | 730 | 1 | Magnesium By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 952 | 1 | Phosphoserine; by PKA By similarity | ||||||
Sequences
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References
| [1] | "Isolation and characterization of cDNA encoding the putative guinea pig distal colon H+, K+ -ATPase alpha subunit." Watanabe T., Sato M., Kaneko K., Suzuki T., Yoshida T., Suzuki Y. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley. |
| [2] | "Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit." Pestov N.B., Romanova L.G., Korneenko T.V., Egorov M.V., Kostina M.B., Sverdlov V.E., Askari A., Shakhparonov M.I., Modyanov N.N. FEBS Lett. 440:320-324(1998) [PubMed: 9872395] [Abstract] Cited for: TISSUE SPECIFICITY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D21854 mRNA. Translation: BAA04880.1. |
| RefSeq | NP_001166387.1. NM_001172916.1. |
3D structure databases | |
| ProteinModelPortal | Q64392. |
| SMR | Q64392. Positions 36-1033. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSCPOT00000003451; ENSCPOP00000003081; ENSCPOG00000003407. |
| GeneID | 100135482. |
Organism-specific databases | |
| CTD | 479. |
Phylogenomic databases | |
| eggNOG | roNOG10939. |
| GeneTree | ENSGT00560000076866. |
| HOVERGEN | HBG004298. |
| InParanoid | Q64392. |
| OMA | ALVIRDS. |
| OrthoDB | EOG41C6VF. |
Family and domain databases | |
| InterPro | IPR023306. ATPase_cation_domN. IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR005775. ATPase_P-typ_cation-ex_asu_euk. IPR006069. ATPase_P-typ_cation-exchng_asu. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR023298. ATPase_P-typ_TM_dom. IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. [Graphical view] |
| Gene3D | G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 1 hit. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit. G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 2 hits. |
| Pfam | PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view] |
| PRINTS | PR00119. CATATPASE. PR00121. NAKATPASE. |
| SMART | SM00831. Cation_ATPase_N. 1 hit. [Graphical view] |
| SUPFAM | SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit. |
| TIGRFAMs | TIGR01106. ATPase-IIC_X-K. 1 hit. TIGR01494. ATPase_P-type. 2 hits. |
| PROSITE | PS00154. ATPASE_E1_E2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | AT12A_CAVPO | ||||||||
| Accession | Primary (citable) accession number: Q64392 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with