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Protein

Potassium-transporting ATPase alpha chain 2

Gene

ATP12A

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei385 – 38514-aspartylphosphate intermediateBy similarity
Metal bindingi726 – 7261MagnesiumBy similarity
Metal bindingi730 – 7301MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 2 (EC:3.6.3.10)
Alternative name(s):
Non-gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:ATP12A
Synonyms:ATP1AL1
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 9695CytoplasmicSequence analysisAdd
BLAST
Transmembranei97 – 11721HelicalSequence analysisAdd
BLAST
Topological domaini118 – 14023LumenalSequence analysisAdd
BLAST
Transmembranei141 – 16121HelicalSequence analysisAdd
BLAST
Topological domaini162 – 297136CytoplasmicSequence analysisAdd
BLAST
Transmembranei298 – 31720HelicalSequence analysisAdd
BLAST
Topological domaini318 – 32912LumenalSequence analysisAdd
BLAST
Transmembranei330 – 34718HelicalSequence analysisAdd
BLAST
Topological domaini348 – 781434CytoplasmicSequence analysisAdd
BLAST
Transmembranei782 – 80120HelicalSequence analysisAdd
BLAST
Topological domaini802 – 81110LumenalSequence analysis
Transmembranei812 – 83221HelicalSequence analysisAdd
BLAST
Topological domaini833 – 85220CytoplasmicSequence analysisAdd
BLAST
Transmembranei853 – 87523HelicalSequence analysisAdd
BLAST
Topological domaini876 – 92752LumenalSequence analysisAdd
BLAST
Transmembranei928 – 94720HelicalSequence analysisAdd
BLAST
Topological domaini948 – 96114CytoplasmicSequence analysisAdd
BLAST
Transmembranei962 – 98019HelicalSequence analysisAdd
BLAST
Topological domaini981 – 99515LumenalSequence analysisAdd
BLAST
Transmembranei996 – 101621HelicalSequence analysisAdd
BLAST
Topological domaini1017 – 103317CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 10331032Potassium-transporting ATPase alpha chain 2PRO_0000046259Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91PhosphotyrosineBy similarity
Modified residuei10 – 101PhosphoserineBy similarity
Modified residuei237 – 2371PhosphoserineBy similarity
Modified residuei284 – 2841PhosphoserineBy similarity
Modified residuei461 – 4611PhosphoserineBy similarity
Modified residuei599 – 5991PhosphoserineBy similarity
Modified residuei838 – 8381PhosphoserineBy similarity
Modified residuei952 – 9521Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ64392.

Expressioni

Tissue specificityi

Found in skin, kidney and distal colon.1 Publication

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000003081.

Structurei

3D structure databases

ProteinModelPortaliQ64392.
SMRiQ64392. Positions 36-1033.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00840000129739.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ64392.
OMAiNQVFDQS.
OrthoDBiEOG091G01BB.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030318. Atp12a.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF282. PTHR24093:SF282. 2 hits.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64392-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRKTLEIYS VELDGTKDTK QLGQEEGKKC NELDLKKSSQ KEELKKELDL
60 70 80 90 100
DDHKLTSEEL EQKYGTNIIR GLSSTRAAEL LARDGPNALS PPKQTPEIIK
110 120 130 140 150
FLKQMIGGFS ILLWVGAILC WIAYGIQYAS NQSGSLDNVY LGVVLALVVI
160 170 180 190 200
LTGIFAYYQE AKSTNIMSSF SKMIPQEALV TRDAEKKVIP AEQLVVGDIV
210 220 230 240 250
EIKGGDQIPA DIRLLFSQGC KVDNSSLTGE SEPQPRSAEF THENPLETKN
260 270 280 290 300
IAFYSTTCLE GTATGMVINT GDRTIIGRIA SLASGVGNEK TPIATEIEHF
310 320 330 340 350
VHIVAGVAVS IGILFFIIAV SLKYRVLDSI IFLIGIIVAN VPEGLLATVT
360 370 380 390 400
VTLSLTAKRM AKKNCLVKNL EAVETLGSTS VICSDKTGTL TQNRMTVAHL
410 420 430 440 450
WFDSQIFTAD TSESQSNQAF DQSSGTWASL SKIIALCNRA EFRPGQENVP
460 470 480 490 500
IMKRVVVGDA SETALLKFSE VILGDVMEIR KRNRKVAEIP FNSTNKFQLS
510 520 530 540 550
IHETEDPGDP RFLMVMKGAP ERILEKCSTI MINGQEQPLD KNNANAFHTA
560 570 580 590 600
YMELGGMGER VLGFCHLYLP AHEFPENYSF DVDTMNFPTS NLCFVGLLSM
610 620 630 640 650
IDPPRSTVPD AVAKCRSAGI KVIMVTGDHP ITAKAIAKSV GIISANSETV
660 670 680 690 700
EDIAKRCNIA VEQVNKQDAR AAVVTGMELK DMTPEQLDEI LANYPEIVFA
710 720 730 740 750
RTSPQQKLII VEGCQRQNAV VAVTGDGVND SPALKKADIG IAMGIAGSDA
760 770 780 790 800
AKNAADMVLL DDNFASIVTG VEEGRLIFDN LKKTIAYTLT KNIAELCPFL
810 820 830 840 850
VYIIVGLPLP IGTITILFID LGTDIIPSIA LAYEKVESDI MNRKPRHKKK
860 870 880 890 900
DRLVNHQLAI YSYLHIGLMQ ALGAFLVYFT VYAQQGFWPT SLIQLRVKWE
910 920 930 940 950
QDYVNDLEDS YGQQWTRYQR KYLEWTGYTA FFVGIMVQQI ADLIIRKTRR
960 970 980 990 1000
NSIFQQGLFR NKVIWVGITS QIIVALILSC GLGSITALNF TMLRVQYWFV
1010 1020 1030
AVPHAILIWV YDEVRKLFLR LYPGSWWDKN MYY
Length:1,033
Mass (Da):114,557
Last modified:November 1, 1996 - v1
Checksum:iC4EEE72D83C1F40C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21854 mRNA. Translation: BAA04880.1.
RefSeqiNP_001166387.1. NM_001172916.1.

Genome annotation databases

EnsembliENSCPOT00000003451; ENSCPOP00000003081; ENSCPOG00000003407.
GeneIDi100135482.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21854 mRNA. Translation: BAA04880.1.
RefSeqiNP_001166387.1. NM_001172916.1.

3D structure databases

ProteinModelPortaliQ64392.
SMRiQ64392. Positions 36-1033.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000003081.

Proteomic databases

PRIDEiQ64392.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCPOT00000003451; ENSCPOP00000003081; ENSCPOG00000003407.
GeneIDi100135482.

Organism-specific databases

CTDi479.

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00840000129739.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ64392.
OMAiNQVFDQS.
OrthoDBiEOG091G01BB.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030318. Atp12a.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF282. PTHR24093:SF282. 2 hits.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT12A_CAVPO
AccessioniPrimary (citable) accession number: Q64392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.