Q64391 (CP1A2_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 1A2
EC=1.14.14.1

Alternative name(s):
CYPIA2

Gene names

Name:

CYP1A2

Organism

Cavia porcellus (Guinea pig) [Reference proteome]

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation By similarity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	alkaloid metabolic process Inferred from electronic annotation. Source: Compara cellular respiration Inferred from electronic annotation. Source: Compara cellular response to cadmium ion Inferred from electronic annotation. Source: Compara dibenzo-p-dioxin metabolic process Inferred from electronic annotation. Source: Compara exogenous drug catabolic process Inferred from electronic annotation. Source: Compara hydrogen peroxide biosynthetic process Inferred from electronic annotation. Source: Compara lung development Inferred from electronic annotation. Source: Compara monocarboxylic acid metabolic process Inferred from electronic annotation. Source: Compara monoterpenoid metabolic process Inferred from electronic annotation. Source: Compara oxidative deethylation Inferred from electronic annotation. Source: Compara oxidative demethylation Inferred from electronic annotation. Source: Compara porphyrin-containing compound metabolic process Inferred from electronic annotation. Source: Compara post-embryonic development Inferred from electronic annotation. Source: Compara regulation of gene expression Inferred from electronic annotation. Source: Compara steroid catabolic process Inferred from electronic annotation. Source: Compara toxin biosynthetic process Inferred from electronic annotation. Source: Compara
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC caffeine oxidase activity Inferred from electronic annotation. Source: Compara demethylase activity Inferred from electronic annotation. Source: Compara electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from sequence or structural similarity. Source: UniProtKB iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 515	514	Cytochrome P450 1A2		PRO_0000051650
Sites
	Metal binding	458	1	Iron (heme axial ligand) By similarity
	Binding site	226	1	Substrate By similarity
Amino acid modifications
	Modified residue	289	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	149	1	V → L in AAB70866. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q64391 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E5EDF0B9D33151A8	FASTA	515	58,423
	10 20 30 40 50 60 MPLSWLLPFS AMELLLTATI FYLVLWVVKA FRLQVPKGLK SPPGPWGWPL IGHVLTLGKN 70 80 90 100 110 120 PHLALTRLSA RYGDVLQIRI GSTPVVVLSG LDTIRQALVR QSDDFKGRPD LYSSTFISDG 130 140 150 160 170 180 QSMIFNPDSG PVWAARRRLA QSALQSFSVA SDPASVSSCY LEEHVSREAE HLVTKLLDLM 190 200 210 220 230 240 AGPGCFEPSS QIVGSVANVI GAMCFGKNFP QTSEEMLQIV NTSKEFTEFA SSGNPVDFFP 250 260 270 280 290 300 ILRYLPNPML QQFKDFNKRF LQFLQKTVQE HYQDFDKNHV QDIASALFKH SEESPHVNGD 310 320 330 340 350 360 LIPRKKIVNL VNDIFGAGFD TVTTAISWSL LYLVTKPEIQ KKIHKELDAV IGRDRKPRLA 370 380 390 400 410 420 DRPQLPYMEA FILEVFRYSS FLPFTIPHCT TRDTILNGFY IPKDRCVFIN QWQVNHDPKQ 430 440 450 460 470 480 WEDPFEFRPE RFLLANNTAV DKTLSDKILL FGLGKRRCIG ETLGRWEVFL FLAILLQQLE 490 500 510 FSVPPGVKVD LTPVYGLTMK PPHCQHVQAR PRFSK

References

[1]	"Regulation of CYP1A and CYP3A mRNAs by ascorbic acid in guinea pigs." Mori T., Itoh S., Ohgiya S., Ishizaki K., Kamataki T. Arch. Biochem. Biophys. 348:268-277(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley. Tissue: Liver.
[2]	Black V.H. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50457 mRNA. Translation: BAA09048.1. U23501 mRNA. Translation: AAB70866.1.
RefSeq	NP_001166165.1. NM_001172694.1.
3D structure databases
ProteinModelPortal	Q64391.
SMR	Q64391. Positions 34-514.
ModBase	Search...
Protein-protein interaction databases
STRING	10141.ENSCPOP00000017610.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCPOT00000001250; ENSCPOP00000017610; ENSCPOG00000001235.
GeneID	100135513.
Organism-specific databases
CTD	1544.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00680000099714.
HOGENOM	HOG000036991.
HOVERGEN	HBG106944.
InParanoid	Q64391.
OrthoDB	EOG4WSW9D.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP1A2_CAVPO

Accession

Primary (citable) accession number: Q64391
Secondary accession number(s): Q64404

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program