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Protein

Cytochrome P450 1A2

Gene

CYP1A2

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation (By similarity).By similarity

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei226 – 2261SubstrateBy similarity
Metal bindingi458 – 4581Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. caffeine oxidase activity Source: Ensembl
  3. demethylase activity Source: Ensembl
  4. heme binding Source: UniProtKB
  5. iron ion binding Source: InterPro

GO - Biological processi

  1. alkaloid metabolic process Source: Ensembl
  2. cellular respiration Source: Ensembl
  3. cellular response to cadmium ion Source: Ensembl
  4. dibenzo-p-dioxin metabolic process Source: Ensembl
  5. exogenous drug catabolic process Source: Ensembl
  6. hydrogen peroxide biosynthetic process Source: Ensembl
  7. lung development Source: Ensembl
  8. monocarboxylic acid metabolic process Source: Ensembl
  9. monoterpenoid metabolic process Source: Ensembl
  10. oxidative deethylation Source: Ensembl
  11. oxidative demethylation Source: Ensembl
  12. porphyrin-containing compound metabolic process Source: Ensembl
  13. post-embryonic development Source: Ensembl
  14. regulation of gene expression Source: Ensembl
  15. steroid catabolic process Source: Ensembl
  16. toxin biosynthetic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A2 (EC:1.14.14.1)
Alternative name(s):
CYPIA2
Gene namesi
Name:CYP1A2
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
ProteomesiUP000005447 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 515514Cytochrome P450 1A2PRO_0000051650Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691O-linked (GlcNAc)By similarity

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000017610.

Structurei

3D structure databases

ProteinModelPortaliQ64391.
SMRiQ64391. Positions 34-514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiQ64391.
OrthoDBiEOG7RBZ85.
TreeFamiTF105095.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLSWLLPFS AMELLLTATI FYLVLWVVKA FRLQVPKGLK SPPGPWGWPL
60 70 80 90 100
IGHVLTLGKN PHLALTRLSA RYGDVLQIRI GSTPVVVLSG LDTIRQALVR
110 120 130 140 150
QSDDFKGRPD LYSSTFISDG QSMIFNPDSG PVWAARRRLA QSALQSFSVA
160 170 180 190 200
SDPASVSSCY LEEHVSREAE HLVTKLLDLM AGPGCFEPSS QIVGSVANVI
210 220 230 240 250
GAMCFGKNFP QTSEEMLQIV NTSKEFTEFA SSGNPVDFFP ILRYLPNPML
260 270 280 290 300
QQFKDFNKRF LQFLQKTVQE HYQDFDKNHV QDIASALFKH SEESPHVNGD
310 320 330 340 350
LIPRKKIVNL VNDIFGAGFD TVTTAISWSL LYLVTKPEIQ KKIHKELDAV
360 370 380 390 400
IGRDRKPRLA DRPQLPYMEA FILEVFRYSS FLPFTIPHCT TRDTILNGFY
410 420 430 440 450
IPKDRCVFIN QWQVNHDPKQ WEDPFEFRPE RFLLANNTAV DKTLSDKILL
460 470 480 490 500
FGLGKRRCIG ETLGRWEVFL FLAILLQQLE FSVPPGVKVD LTPVYGLTMK
510
PPHCQHVQAR PRFSK
Length:515
Mass (Da):58,423
Last modified:November 1, 1996 - v1
Checksum:iE5EDF0B9D33151A8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti149 – 1491V → L in AAB70866 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50457 mRNA. Translation: BAA09048.1.
U23501 mRNA. Translation: AAB70866.1.
RefSeqiNP_001166165.1. NM_001172694.1.

Genome annotation databases

GeneIDi100135513.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50457 mRNA. Translation: BAA09048.1.
U23501 mRNA. Translation: AAB70866.1.
RefSeqiNP_001166165.1. NM_001172694.1.

3D structure databases

ProteinModelPortaliQ64391.
SMRiQ64391. Positions 34-514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000017610.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100135513.

Organism-specific databases

CTDi1544.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiQ64391.
OrthoDBiEOG7RBZ85.
TreeFamiTF105095.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Regulation of CYP1A and CYP3A mRNAs by ascorbic acid in guinea pigs."
    Mori T., Itoh S., Ohgiya S., Ishizaki K., Kamataki T.
    Arch. Biochem. Biophys. 348:268-277(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Hartley.
    Tissue: Liver.
  2. Black V.H.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Hartley.
    Tissue: Liver.

Entry informationi

Entry nameiCP1A2_CAVPO
AccessioniPrimary (citable) accession number: Q64391
Secondary accession number(s): Q64404
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 4, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.