Reviewed, UniProtKB/Swiss-Prot Q64391 (CP1A2_CAVPO)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 1A2
    EC=1.14.14.1
Alternative name(s):
    CYPIA2

Gene names

Name:

CYP1A2

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation By similarity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	Cytochrome P450 1A2		PRO_0000051650
Sites
	Metal binding	458	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Modified residue	289	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	149	1	V → L in AAB70866. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q64391-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E5EDF0B9D33151A8	FASTA	515	58,423
	10 20 30 40 50 60 MPLSWLLPFS AMELLLTATI FYLVLWVVKA FRLQVPKGLK SPPGPWGWPL IGHVLTLGKN 70 80 90 100 110 120 PHLALTRLSA RYGDVLQIRI GSTPVVVLSG LDTIRQALVR QSDDFKGRPD LYSSTFISDG 130 140 150 160 170 180 QSMIFNPDSG PVWAARRRLA QSALQSFSVA SDPASVSSCY LEEHVSREAE HLVTKLLDLM 190 200 210 220 230 240 AGPGCFEPSS QIVGSVANVI GAMCFGKNFP QTSEEMLQIV NTSKEFTEFA SSGNPVDFFP 250 260 270 280 290 300 ILRYLPNPML QQFKDFNKRF LQFLQKTVQE HYQDFDKNHV QDIASALFKH SEESPHVNGD 310 320 330 340 350 360 LIPRKKIVNL VNDIFGAGFD TVTTAISWSL LYLVTKPEIQ KKIHKELDAV IGRDRKPRLA 370 380 390 400 410 420 DRPQLPYMEA FILEVFRYSS FLPFTIPHCT TRDTILNGFY IPKDRCVFIN QWQVNHDPKQ 430 440 450 460 470 480 WEDPFEFRPE RFLLANNTAV DKTLSDKILL FGLGKRRCIG ETLGRWEVFL FLAILLQQLE 490 500 510 FSVPPGVKVD LTPVYGLTMK PPHCQHVQAR PRFSK

References

[1]	"Regulation of CYP1A and CYP3A mRNAs by ascorbic acid in guinea pigs." Mori T., Itoh S., Ohgiya S., Ishizaki K., Kamataki T. Arch. Biochem. Biophys. 348:268-277(1997) [PubMed: 9434738] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley. Tissue: Liver.
[2]	Black V.H. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50457 mRNA. Translation: BAA09048.1. U23501 mRNA. Translation: AAB70866.1.
3D structure databases
SMR	Q64391. Positions 34-514.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCPOT00000001250; ENSCPOP00000017610; ENSCPOG00000001235; Cavia porcellus. [Genome view]
Phylogenomic databases
HOVERGEN	Q64391.
InParanoid	Q64391.
Enzyme and pathway databases
BRENDA	1.14.14.1. 44.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit. PTHR19383:SF63. Cyt_P450_E_grp-I_CYP1. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP1A2_CAVPO

Accession

Primary (citable) accession number: Q64391
Secondary accession number(s): Q64404

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 15, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)