Q64387 (PNOC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prepronociceptin

Alternative name(s):
N23K/N27K

Cleaved into the following 3 chains:

1. Neuropeptide 1
2. Nociceptin
   Alternative name(s):
   Orphanin FQ
   PPNOC
3. Neuropeptide 2

Gene names

Name:	Pnoc
Synonyms:	Npnc1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. When administered intracerebroventricularly, nociceptin induces hyperalgesia and decreases locomotor activity.
Subcellular location	Secreted.
Tissue specificity	Brain and spinal cord. Low levels in kidney and spleen.
Developmental stage	In embryonic brain, first detected at day 14 and in postnatal brain, levels increase in day 1 and day 18. Levels decrease significantly in adults.
Post-translational modification	Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin. The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Sequence similarities	Belongs to the opioid neuropeptide precursor family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Molecular function	Neuropeptide Neurotransmitter Opioid peptide
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW synaptic transmission Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Short (identifier: Q64387-1)

Also known as: N23K;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Long (identifier: Q64387-2)

Also known as: N27K;

The sequence of this isoform differs from the canonical sequence as follows:
     186-187: NV → IQVIPRTACVHSKTCRPGVRIPPSPRH

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 95	76			PRO_0000008330
	Peptide	98 – 138	41	Neuropeptide 1 Probable		PRO_0000008331
	Peptide	141 – 157	17	Nociceptin		PRO_0000008332
	Peptide	160 – 176	17	Neuropeptide 2 Probable		PRO_0000008333
	Propeptide	180 – 187	8			PRO_0000008334
Regions
	Repeat	109 – 114	6	1
	Repeat	115 – 120	6	2
	Repeat	121 – 126	6	3
	Region	109 – 126	18	3 X 6 AA tandem repeats of D-A-E-P-G-A
Natural variations
	Alternative sequence	186 – 187	2	NV → IQVIPRTACVHSKTCRPGVR IPPSPRH in isoform Long.		VSP_001446
Experimental info
	Sequence conflict	80	1	V → T in AAC52581. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform Short (N23K) [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 81A8BFBEF2DCB913	FASTA	187	20,884
	10 20 30 40 50 60 MKILFCDVLL LSLLSSVFSS CPRDCLTCQE KLHPAPDSFN LKTCILQCEE KVFPRPLWTV 70 80 90 100 110 120 CTKVMASGSG QLSPADPELV SAALYQPKAS EMQHLKRMPR VRSLVQVRDA EPGADAEPGA 130 140 150 160 170 180 DAEPGADDAE EVEQKQLQKR FGGFTGARKS ARKLANQKRF SEFMRQYLVL SMQSSQRRRT LHQNGNV
	Isoform Long (N27K) [UniParc]. Checksum: 759A547E18A96E16	FASTA	212	23,659
	10 20 30 40 50 60 MKILFCDVLL LSLLSSVFSS CPRDCLTCQE KLHPAPDSFN LKTCILQCEE KVFPRPLWTV 70 80 90 100 110 120 CTKVMASGSG QLSPADPELV SAALYQPKAS EMQHLKRMPR VRSLVQVRDA EPGADAEPGA 130 140 150 160 170 180 DAEPGADDAE EVEQKQLQKR FGGFTGARKS ARKLANQKRF SEFMRQYLVL SMQSSQRRRT 190 200 210 LHQNGIQVIP RTACVHSKTC RPGVRIPPSP RH

References

[1]	"Structure and regional distribution of nociceptin/orphanin FQ precursor." Houtani T., Nishi M., Takeshima H., Nukada T., Sugimoto T. Biochem. Biophys. Res. Commun. 219:714-719(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT). Tissue: Brain.
[2]	"N23K, a gene transiently up-regulated during neural differentiation, encodes a precursor protein for a newly identified neuropeptide nociceptin." Saito Y., Maruyama K., Saido T.C., Kawashima S. Biochem. Biophys. Res. Commun. 217:539-545(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[3]	"Structure, tissue distribution, and chromosomal localization of the prepronociceptin gene." Mollereau C., Simons M.-J., Soularue P., Liners F., Vassart G., Meunier J.-C., Parmentier M. Proc. Natl. Acad. Sci. U.S.A. 93:8666-8670(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT). Strain: 129/Sv.
[4]	"Molecular cloning and characterization of a novel form of neuropeptide gene as a developmentally regulated molecule." Saito Y., Maruyama K., Kawano H., Hagino-Yamagishi K., Kawamura K., Saido T.C., Kawashima S. J. Biol. Chem. 271:15615-15622(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
[5]	"Cloning and expression of a cDNA encoding a mouse brain orphanin FQ/nociceptin precursor." Pan Y.-X., Xu J., Pasternak G.W. Biochem. J. 315:11-13(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 79-187. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D82866 mRNA. Translation: BAA11614.1. D50056 mRNA. Translation: BAA08774.1. X97373 mRNA. Translation: CAA66042.1. X97371, X97372 Genomic DNA. Translation: CAA66041.1. U44027 mRNA. Translation: AAC52581.1. D50055 mRNA. Translation: BAA08773.1.
IPI	IPI00133477. IPI00222450.
PIR	JC4502. S70631.
RefSeq	NP_001192004.1. NM_001205075.1. NP_035062.1. NM_010932.2.
UniGene	Mm.16347. Mm.486163.
3D structure databases
ProteinModelPortal	Q64387.
ModBase	Search...
PTM databases
PhosphoSite	Q64387.
Proteomic databases
PRIDE	Q64387.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000059339; ENSMUSP00000054210; ENSMUSG00000045731.
GeneID	18155.
KEGG	mmu:18155.
Organism-specific databases
CTD	5368.
MGI	MGI:105308. Pnoc.
Phylogenomic databases
eggNOG	NOG40726.
GeneTree	ENSGT00510000049153.
HOVERGEN	HBG008227.
InParanoid	Q64387.
OMA	HLKRMPR.
OrthoDB	EOG44BB3B.
Gene expression databases
ArrayExpress	Q64387.
Bgee	Q64387.
CleanEx	MM_PNOC.
Genevestigator	Q64387.
GermOnline	ENSMUSG00000045731. Mus musculus.
Family and domain databases
InterPro	IPR002367. Nociceptin. IPR006024. Opioid_neupept. [Graphical view]
PANTHER	PTHR11438. PTHR11438. 1 hit.
Pfam	PF01160. Opiods_neuropep. 1 hit. [Graphical view]
PRINTS	PR01028. OPIOIDPRCRSR. PR01031. ORPHNNPRCRSR.
PROSITE	PS01252. OPIOIDS_PRECURSOR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PNOC. mouse.
NextBio	293420.
SOURCE	Search...

Entry information

Entry name

PNOC_MOUSE

Accession

Primary (citable) accession number: Q64387
Secondary accession number(s): Q61105, Q61938

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program