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Q64387

- PNOC_MOUSE

UniProt

Q64387 - PNOC_MOUSE

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Protein

Prepronociceptin

Gene

Pnoc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. When administered intracerebroventricularly, nociceptin induces hyperalgesia and decreases locomotor activity.

GO - Biological processi

  1. neuropeptide signaling pathway Source: UniProtKB-KW
  2. synaptic transmission Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Neuropeptide, Neurotransmitter, Opioid peptide

Names & Taxonomyi

Protein namesi
Recommended name:
Prepronociceptin
Alternative name(s):
N23K/N27K
Cleaved into the following 3 chains:
Alternative name(s):
Orphanin FQ
PPNOC
Gene namesi
Name:Pnoc
Synonyms:Npnc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:105308. Pnoc.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Propeptidei20 – 9576PRO_0000008330Add
BLAST
Peptidei98 – 13841Neuropeptide 1CuratedPRO_0000008331Add
BLAST
Peptidei141 – 15717NociceptinPRO_0000008332Add
BLAST
Peptidei160 – 17617Neuropeptide 2CuratedPRO_0000008333Add
BLAST
Propeptidei180 – 1878PRO_0000008334

Post-translational modificationi

Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin.
The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

MaxQBiQ64387.
PRIDEiQ64387.

PTM databases

PhosphoSiteiQ64387.

Expressioni

Tissue specificityi

Brain and spinal cord. Low levels in kidney and spleen.

Developmental stagei

In embryonic brain, first detected at day 14 and in postnatal brain, levels increase in day 1 and day 18. Levels decrease significantly in adults.

Gene expression databases

BgeeiQ64387.
CleanExiMM_PNOC.
ExpressionAtlasiQ64387. baseline and differential.
GenevestigatoriQ64387.

Structurei

3D structure databases

ProteinModelPortaliQ64387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati109 – 11461
Repeati115 – 12062
Repeati121 – 12663

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni109 – 126183 X 6 AA tandem repeats of D-A-E-P-G-AAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG40726.
GeneTreeiENSGT00510000049153.
HOVERGENiHBG008227.
InParanoidiQ64387.
OMAiHLKRMPR.
OrthoDBiEOG7MH118.
PhylomeDBiQ64387.
TreeFamiTF332620.

Family and domain databases

InterProiIPR002367. Nociceptin.
IPR006024. Opioid_neupept.
[Graphical view]
PANTHERiPTHR11438. PTHR11438. 1 hit.
PfamiPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSiPR01028. OPIOIDPRCRSR.
PR01031. ORPHNNPRCRSR.
PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Short (identifier: Q64387) [UniParc]FASTAAdd to Basket

Also known as: N23K

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKILFCDVLL LSLLSSVFSS CPRDCLTCQE KLHPAPDSFN LKTCILQCEE
60 70 80 90 100
KVFPRPLWTV CTKVMASGSG QLSPADPELV SAALYQPKAS EMQHLKRMPR
110 120 130 140 150
VRSLVQVRDA EPGADAEPGA DAEPGADDAE EVEQKQLQKR FGGFTGARKS
160 170 180
ARKLANQKRF SEFMRQYLVL SMQSSQRRRT LHQNGNV
Length:187
Mass (Da):20,884
Last modified:November 1, 1997 - v1
Checksum:i81A8BFBEF2DCB913
GO
Isoform Long (identifier: Q64387-2) [UniParc]FASTAAdd to Basket

Also known as: N27K

The sequence of this isoform differs from the canonical sequence as follows:
     186-187: NV → IQVIPRTACVHSKTCRPGVRIPPSPRH

Note: No experimental confirmation available.

Show »
Length:212
Mass (Da):23,659
Checksum:i759A547E18A96E16
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti80 – 801V → T in AAC52581. (PubMed:8670093)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei186 – 1872NV → IQVIPRTACVHSKTCRPGVR IPPSPRH in isoform Long. 1 PublicationVSP_001446

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D82866 mRNA. Translation: BAA11614.1.
D50056 mRNA. Translation: BAA08774.1.
X97373 mRNA. Translation: CAA66042.1.
X97371, X97372 Genomic DNA. Translation: CAA66041.1.
U44027 mRNA. Translation: AAC52581.1.
D50055 mRNA. Translation: BAA08773.1.
CCDSiCCDS27213.1. [Q64387-1]
PIRiJC4502.
S70631.
RefSeqiNP_001192004.1. NM_001205075.1. [Q64387-2]
NP_035062.1. NM_010932.2. [Q64387-1]
XP_006518747.1. XM_006518684.1. [Q64387-2]
UniGeneiMm.16347.
Mm.486163.

Genome annotation databases

EnsembliENSMUST00000059339; ENSMUSP00000054210; ENSMUSG00000045731. [Q64387-1]
GeneIDi18155.
KEGGimmu:18155.
UCSCiuc007uji.2. mouse. [Q64387-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D82866 mRNA. Translation: BAA11614.1 .
D50056 mRNA. Translation: BAA08774.1 .
X97373 mRNA. Translation: CAA66042.1 .
X97371 , X97372 Genomic DNA. Translation: CAA66041.1 .
U44027 mRNA. Translation: AAC52581.1 .
D50055 mRNA. Translation: BAA08773.1 .
CCDSi CCDS27213.1. [Q64387-1 ]
PIRi JC4502.
S70631.
RefSeqi NP_001192004.1. NM_001205075.1. [Q64387-2 ]
NP_035062.1. NM_010932.2. [Q64387-1 ]
XP_006518747.1. XM_006518684.1. [Q64387-2 ]
UniGenei Mm.16347.
Mm.486163.

3D structure databases

ProteinModelPortali Q64387.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q64387.

Proteomic databases

MaxQBi Q64387.
PRIDEi Q64387.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000059339 ; ENSMUSP00000054210 ; ENSMUSG00000045731 . [Q64387-1 ]
GeneIDi 18155.
KEGGi mmu:18155.
UCSCi uc007uji.2. mouse. [Q64387-1 ]

Organism-specific databases

CTDi 5368.
MGIi MGI:105308. Pnoc.

Phylogenomic databases

eggNOGi NOG40726.
GeneTreei ENSGT00510000049153.
HOVERGENi HBG008227.
InParanoidi Q64387.
OMAi HLKRMPR.
OrthoDBi EOG7MH118.
PhylomeDBi Q64387.
TreeFami TF332620.

Miscellaneous databases

ChiTaRSi PNOC. mouse.
NextBioi 293420.
PROi Q64387.
SOURCEi Search...

Gene expression databases

Bgeei Q64387.
CleanExi MM_PNOC.
ExpressionAtlasi Q64387. baseline and differential.
Genevestigatori Q64387.

Family and domain databases

InterProi IPR002367. Nociceptin.
IPR006024. Opioid_neupept.
[Graphical view ]
PANTHERi PTHR11438. PTHR11438. 1 hit.
Pfami PF01160. Opiods_neuropep. 1 hit.
[Graphical view ]
PRINTSi PR01028. OPIOIDPRCRSR.
PR01031. ORPHNNPRCRSR.
PROSITEi PS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure and regional distribution of nociceptin/orphanin FQ precursor."
    Houtani T., Nishi M., Takeshima H., Nukada T., Sugimoto T.
    Biochem. Biophys. Res. Commun. 219:714-719(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    Tissue: Brain.
  2. "N23K, a gene transiently up-regulated during neural differentiation, encodes a precursor protein for a newly identified neuropeptide nociceptin."
    Saito Y., Maruyama K., Saido T.C., Kawashima S.
    Biochem. Biophys. Res. Commun. 217:539-545(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
  3. "Structure, tissue distribution, and chromosomal localization of the prepronociceptin gene."
    Mollereau C., Simons M.-J., Soularue P., Liners F., Vassart G., Meunier J.-C., Parmentier M.
    Proc. Natl. Acad. Sci. U.S.A. 93:8666-8670(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT).
    Strain: 129/Sv.
  4. "Molecular cloning and characterization of a novel form of neuropeptide gene as a developmentally regulated molecule."
    Saito Y., Maruyama K., Kawano H., Hagino-Yamagishi K., Kawamura K., Saido T.C., Kawashima S.
    J. Biol. Chem. 271:15615-15622(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
  5. "Cloning and expression of a cDNA encoding a mouse brain orphanin FQ/nociceptin precursor."
    Pan Y.-X., Xu J., Pasternak G.W.
    Biochem. J. 315:11-13(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 79-187.
    Tissue: Brain.

Entry informationi

Entry nameiPNOC_MOUSE
AccessioniPrimary (citable) accession number: Q64387
Secondary accession number(s): Q61105, Q61938
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3