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Q64387

- PNOC_MOUSE

UniProt

Q64387 - PNOC_MOUSE

Protein

Prepronociceptin

Gene

Pnoc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. When administered intracerebroventricularly, nociceptin induces hyperalgesia and decreases locomotor activity.

    GO - Biological processi

    1. neuropeptide signaling pathway Source: UniProtKB-KW
    2. synaptic transmission Source: UniProtKB-KW

    Keywords - Molecular functioni

    Neuropeptide, Neurotransmitter, Opioid peptide

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prepronociceptin
    Alternative name(s):
    N23K/N27K
    Cleaved into the following 3 chains:
    Alternative name(s):
    Orphanin FQ
    PPNOC
    Gene namesi
    Name:Pnoc
    Synonyms:Npnc1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:105308. Pnoc.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Propeptidei20 – 9576PRO_0000008330Add
    BLAST
    Peptidei98 – 13841Neuropeptide 1CuratedPRO_0000008331Add
    BLAST
    Peptidei141 – 15717NociceptinPRO_0000008332Add
    BLAST
    Peptidei160 – 17617Neuropeptide 2CuratedPRO_0000008333Add
    BLAST
    Propeptidei180 – 1878PRO_0000008334

    Post-translational modificationi

    Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin.
    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Proteomic databases

    PRIDEiQ64387.

    PTM databases

    PhosphoSiteiQ64387.

    Expressioni

    Tissue specificityi

    Brain and spinal cord. Low levels in kidney and spleen.

    Developmental stagei

    In embryonic brain, first detected at day 14 and in postnatal brain, levels increase in day 1 and day 18. Levels decrease significantly in adults.

    Gene expression databases

    BgeeiQ64387.
    CleanExiMM_PNOC.
    GenevestigatoriQ64387.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64387.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati109 – 11461
    Repeati115 – 12062
    Repeati121 – 12663

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni109 – 126183 X 6 AA tandem repeats of D-A-E-P-G-AAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG40726.
    GeneTreeiENSGT00510000049153.
    HOVERGENiHBG008227.
    InParanoidiQ64387.
    OMAiHLKRMPR.
    OrthoDBiEOG7MH118.
    PhylomeDBiQ64387.
    TreeFamiTF332620.

    Family and domain databases

    InterProiIPR002367. Nociceptin.
    IPR006024. Opioid_neupept.
    [Graphical view]
    PANTHERiPTHR11438. PTHR11438. 1 hit.
    PfamiPF01160. Opiods_neuropep. 1 hit.
    [Graphical view]
    PRINTSiPR01028. OPIOIDPRCRSR.
    PR01031. ORPHNNPRCRSR.
    PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Short (identifier: Q64387-1) [UniParc]FASTAAdd to Basket

    Also known as: N23K

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKILFCDVLL LSLLSSVFSS CPRDCLTCQE KLHPAPDSFN LKTCILQCEE    50
    KVFPRPLWTV CTKVMASGSG QLSPADPELV SAALYQPKAS EMQHLKRMPR 100
    VRSLVQVRDA EPGADAEPGA DAEPGADDAE EVEQKQLQKR FGGFTGARKS 150
    ARKLANQKRF SEFMRQYLVL SMQSSQRRRT LHQNGNV 187
    Length:187
    Mass (Da):20,884
    Last modified:November 1, 1997 - v1
    Checksum:i81A8BFBEF2DCB913
    GO
    Isoform Long (identifier: Q64387-2) [UniParc]FASTAAdd to Basket

    Also known as: N27K

    The sequence of this isoform differs from the canonical sequence as follows:
         186-187: NV → IQVIPRTACVHSKTCRPGVRIPPSPRH

    Note: No experimental confirmation available.

    Show »
    Length:212
    Mass (Da):23,659
    Checksum:i759A547E18A96E16
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti80 – 801V → T in AAC52581. (PubMed:8670093)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei186 – 1872NV → IQVIPRTACVHSKTCRPGVR IPPSPRH in isoform Long. 1 PublicationVSP_001446

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D82866 mRNA. Translation: BAA11614.1.
    D50056 mRNA. Translation: BAA08774.1.
    X97373 mRNA. Translation: CAA66042.1.
    X97371, X97372 Genomic DNA. Translation: CAA66041.1.
    U44027 mRNA. Translation: AAC52581.1.
    D50055 mRNA. Translation: BAA08773.1.
    CCDSiCCDS27213.1. [Q64387-1]
    PIRiJC4502.
    S70631.
    RefSeqiNP_001192004.1. NM_001205075.1. [Q64387-2]
    NP_035062.1. NM_010932.2. [Q64387-1]
    XP_006518747.1. XM_006518684.1. [Q64387-2]
    UniGeneiMm.16347.
    Mm.486163.

    Genome annotation databases

    EnsembliENSMUST00000059339; ENSMUSP00000054210; ENSMUSG00000045731. [Q64387-1]
    GeneIDi18155.
    KEGGimmu:18155.
    UCSCiuc007uji.2. mouse. [Q64387-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D82866 mRNA. Translation: BAA11614.1 .
    D50056 mRNA. Translation: BAA08774.1 .
    X97373 mRNA. Translation: CAA66042.1 .
    X97371 , X97372 Genomic DNA. Translation: CAA66041.1 .
    U44027 mRNA. Translation: AAC52581.1 .
    D50055 mRNA. Translation: BAA08773.1 .
    CCDSi CCDS27213.1. [Q64387-1 ]
    PIRi JC4502.
    S70631.
    RefSeqi NP_001192004.1. NM_001205075.1. [Q64387-2 ]
    NP_035062.1. NM_010932.2. [Q64387-1 ]
    XP_006518747.1. XM_006518684.1. [Q64387-2 ]
    UniGenei Mm.16347.
    Mm.486163.

    3D structure databases

    ProteinModelPortali Q64387.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q64387.

    Proteomic databases

    PRIDEi Q64387.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000059339 ; ENSMUSP00000054210 ; ENSMUSG00000045731 . [Q64387-1 ]
    GeneIDi 18155.
    KEGGi mmu:18155.
    UCSCi uc007uji.2. mouse. [Q64387-1 ]

    Organism-specific databases

    CTDi 5368.
    MGIi MGI:105308. Pnoc.

    Phylogenomic databases

    eggNOGi NOG40726.
    GeneTreei ENSGT00510000049153.
    HOVERGENi HBG008227.
    InParanoidi Q64387.
    OMAi HLKRMPR.
    OrthoDBi EOG7MH118.
    PhylomeDBi Q64387.
    TreeFami TF332620.

    Miscellaneous databases

    ChiTaRSi PNOC. mouse.
    NextBioi 293420.
    PROi Q64387.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q64387.
    CleanExi MM_PNOC.
    Genevestigatori Q64387.

    Family and domain databases

    InterProi IPR002367. Nociceptin.
    IPR006024. Opioid_neupept.
    [Graphical view ]
    PANTHERi PTHR11438. PTHR11438. 1 hit.
    Pfami PF01160. Opiods_neuropep. 1 hit.
    [Graphical view ]
    PRINTSi PR01028. OPIOIDPRCRSR.
    PR01031. ORPHNNPRCRSR.
    PROSITEi PS01252. OPIOIDS_PRECURSOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and regional distribution of nociceptin/orphanin FQ precursor."
      Houtani T., Nishi M., Takeshima H., Nukada T., Sugimoto T.
      Biochem. Biophys. Res. Commun. 219:714-719(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
      Tissue: Brain.
    2. "N23K, a gene transiently up-regulated during neural differentiation, encodes a precursor protein for a newly identified neuropeptide nociceptin."
      Saito Y., Maruyama K., Saido T.C., Kawashima S.
      Biochem. Biophys. Res. Commun. 217:539-545(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    3. "Structure, tissue distribution, and chromosomal localization of the prepronociceptin gene."
      Mollereau C., Simons M.-J., Soularue P., Liners F., Vassart G., Meunier J.-C., Parmentier M.
      Proc. Natl. Acad. Sci. U.S.A. 93:8666-8670(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT).
      Strain: 129/Sv.
    4. "Molecular cloning and characterization of a novel form of neuropeptide gene as a developmentally regulated molecule."
      Saito Y., Maruyama K., Kawano H., Hagino-Yamagishi K., Kawamura K., Saido T.C., Kawashima S.
      J. Biol. Chem. 271:15615-15622(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    5. "Cloning and expression of a cDNA encoding a mouse brain orphanin FQ/nociceptin precursor."
      Pan Y.-X., Xu J., Pasternak G.W.
      Biochem. J. 315:11-13(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 79-187.
      Tissue: Brain.

    Entry informationi

    Entry nameiPNOC_MOUSE
    AccessioniPrimary (citable) accession number: Q64387
    Secondary accession number(s): Q61105, Q61938
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3