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Q64387 (PNOC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prepronociceptin
Alternative name(s):
N23K/N27K

Cleaved into the following 3 chains:

  1. Neuropeptide 1
  2. Nociceptin
    Alternative name(s):
    Orphanin FQ
    PPNOC
  3. Neuropeptide 2
Gene names
Name:Pnoc
Synonyms:Npnc1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. When administered intracerebroventricularly, nociceptin induces hyperalgesia and decreases locomotor activity.

Subcellular location

Secreted.

Tissue specificity

Brain and spinal cord. Low levels in kidney and spleen.

Developmental stage

In embryonic brain, first detected at day 14 and in postnatal brain, levels increase in day 1 and day 18. Levels decrease significantly in adults.

Post-translational modification

Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin.

The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Sequence similarities

Belongs to the opioid neuropeptide precursor family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainRepeat
Signal
   Molecular functionNeuropeptide
Neurotransmitter
Opioid peptide
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processneuropeptide signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

synaptic transmission

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Short (identifier: Q64387-1)

Also known as: N23K;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Long (identifier: Q64387-2)

Also known as: N27K;

The sequence of this isoform differs from the canonical sequence as follows:
     186-187: NV → IQVIPRTACVHSKTCRPGVRIPPSPRH
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Propeptide20 – 9576
PRO_0000008330
Peptide98 – 13841Neuropeptide 1 Probable
PRO_0000008331
Peptide141 – 15717Nociceptin
PRO_0000008332
Peptide160 – 17617Neuropeptide 2 Probable
PRO_0000008333
Propeptide180 – 1878
PRO_0000008334

Regions

Repeat109 – 11461
Repeat115 – 12062
Repeat121 – 12663
Region109 – 126183 X 6 AA tandem repeats of D-A-E-P-G-A

Natural variations

Alternative sequence186 – 1872NV → IQVIPRTACVHSKTCRPGVR IPPSPRH in isoform Long.
VSP_001446

Experimental info

Sequence conflict801V → T in AAC52581. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform Short (N23K) [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 81A8BFBEF2DCB913

FASTA18720,884
        10         20         30         40         50         60 
MKILFCDVLL LSLLSSVFSS CPRDCLTCQE KLHPAPDSFN LKTCILQCEE KVFPRPLWTV 

        70         80         90        100        110        120 
CTKVMASGSG QLSPADPELV SAALYQPKAS EMQHLKRMPR VRSLVQVRDA EPGADAEPGA 

       130        140        150        160        170        180 
DAEPGADDAE EVEQKQLQKR FGGFTGARKS ARKLANQKRF SEFMRQYLVL SMQSSQRRRT 


LHQNGNV 

« Hide

Isoform Long (N27K) [UniParc].

Checksum: 759A547E18A96E16
Show »

FASTA21223,659

References

[1]"Structure and regional distribution of nociceptin/orphanin FQ precursor."
Houtani T., Nishi M., Takeshima H., Nukada T., Sugimoto T.
Biochem. Biophys. Res. Commun. 219:714-719(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
Tissue: Brain.
[2]"N23K, a gene transiently up-regulated during neural differentiation, encodes a precursor protein for a newly identified neuropeptide nociceptin."
Saito Y., Maruyama K., Saido T.C., Kawashima S.
Biochem. Biophys. Res. Commun. 217:539-545(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[3]"Structure, tissue distribution, and chromosomal localization of the prepronociceptin gene."
Mollereau C., Simons M.-J., Soularue P., Liners F., Vassart G., Meunier J.-C., Parmentier M.
Proc. Natl. Acad. Sci. U.S.A. 93:8666-8670(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT).
Strain: 129/Sv.
[4]"Molecular cloning and characterization of a novel form of neuropeptide gene as a developmentally regulated molecule."
Saito Y., Maruyama K., Kawano H., Hagino-Yamagishi K., Kawamura K., Saido T.C., Kawashima S.
J. Biol. Chem. 271:15615-15622(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
[5]"Cloning and expression of a cDNA encoding a mouse brain orphanin FQ/nociceptin precursor."
Pan Y.-X., Xu J., Pasternak G.W.
Biochem. J. 315:11-13(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 79-187.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D82866 mRNA. Translation: BAA11614.1.
D50056 mRNA. Translation: BAA08774.1.
X97373 mRNA. Translation: CAA66042.1.
X97371, X97372 Genomic DNA. Translation: CAA66041.1.
U44027 mRNA. Translation: AAC52581.1.
D50055 mRNA. Translation: BAA08773.1.
PIRJC4502.
S70631.
RefSeqNP_001192004.1. NM_001205075.1.
NP_035062.1. NM_010932.2.
XP_006518747.1. XM_006518684.1.
UniGeneMm.16347.
Mm.486163.

3D structure databases

ProteinModelPortalQ64387.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ64387.

Proteomic databases

PRIDEQ64387.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000059339; ENSMUSP00000054210; ENSMUSG00000045731. [Q64387-1]
GeneID18155.
KEGGmmu:18155.
UCSCuc007uji.2. mouse. [Q64387-1]

Organism-specific databases

CTD5368.
MGIMGI:105308. Pnoc.

Phylogenomic databases

eggNOGNOG40726.
GeneTreeENSGT00510000049153.
HOVERGENHBG008227.
InParanoidQ64387.
OMAHLKRMPR.
OrthoDBEOG7MH118.
PhylomeDBQ64387.
TreeFamTF332620.

Gene expression databases

BgeeQ64387.
CleanExMM_PNOC.
GenevestigatorQ64387.

Family and domain databases

InterProIPR002367. Nociceptin.
IPR006024. Opioid_neupept.
[Graphical view]
PANTHERPTHR11438. PTHR11438. 1 hit.
PfamPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSPR01028. OPIOIDPRCRSR.
PR01031. ORPHNNPRCRSR.
PROSITEPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPNOC. mouse.
NextBio293420.
PROQ64387.
SOURCESearch...

Entry information

Entry namePNOC_MOUSE
AccessionPrimary (citable) accession number: Q64387
Secondary accession number(s): Q61105, Q61938
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot