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Protein

Prepronociceptin

Gene

Pnoc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Nociceptin: Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. When administered intracerebroventricularly, nociceptin induces hyperalgesia and decreases locomotor activity.
Nocistatin: Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia.By similarity
Orphanin FQ2: Has potent analgesic activity.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Neuropeptide, Neurotransmitter, Opioid peptide

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Prepronociceptin
Alternative name(s):
N23K/N27K
Cleaved into the following 3 chains:
Alternative name(s):
Orphanin FQ
PPNOC
Gene namesi
Name:Pnoc
Synonyms:Npnc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:105308. Pnoc.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000000833020 – 95Add BLAST76
PeptideiPRO_000000833198 – 138NocistatinCuratedAdd BLAST41
PeptideiPRO_0000008332141 – 157NociceptinBy similarityAdd BLAST17
PeptideiPRO_0000008333160 – 176Orphanin FQ2CuratedAdd BLAST17
PropeptideiPRO_0000008334180 – 1878

Post-translational modificationi

Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin.
The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiQ64387.
PRIDEiQ64387.

PTM databases

iPTMnetiQ64387.
PhosphoSitePlusiQ64387.

Expressioni

Tissue specificityi

Brain and spinal cord. Low levels in kidney and spleen.

Developmental stagei

In embryonic brain, first detected at day 14 and in postnatal brain, levels increase in day 1 and day 18. Levels decrease significantly in adults.

Gene expression databases

BgeeiENSMUSG00000045731.
CleanExiMM_PNOC.
ExpressionAtlasiQ64387. baseline and differential.
GenevisibleiQ64387. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054210.

Structurei

3D structure databases

ProteinModelPortaliQ64387.
SMRiQ64387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati109 – 11416
Repeati115 – 12026
Repeati121 – 12636

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni109 – 1263 X 6 AA tandem repeats of D-A-E-P-G-AAdd BLAST18

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IY0N. Eukaryota.
ENOG4111JDY. LUCA.
GeneTreeiENSGT00510000049153.
HOVERGENiHBG008227.
InParanoidiQ64387.
OMAiGCKSSAS.
OrthoDBiEOG091G0WQ7.
PhylomeDBiQ64387.
TreeFamiTF332620.

Family and domain databases

InterProiIPR002367. Nociceptin.
IPR006024. Opioid_neupept.
[Graphical view]
PANTHERiPTHR11438. PTHR11438. 1 hit.
PfamiPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSiPR01028. OPIOIDPRCRSR.
PR01031. ORPHNNPRCRSR.
PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Short (identifier: Q64387-1) [UniParc]FASTAAdd to basket
Also known as: N23K

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKILFCDVLL LSLLSSVFSS CPRDCLTCQE KLHPAPDSFN LKTCILQCEE
60 70 80 90 100
KVFPRPLWTV CTKVMASGSG QLSPADPELV SAALYQPKAS EMQHLKRMPR
110 120 130 140 150
VRSLVQVRDA EPGADAEPGA DAEPGADDAE EVEQKQLQKR FGGFTGARKS
160 170 180
ARKLANQKRF SEFMRQYLVL SMQSSQRRRT LHQNGNV
Length:187
Mass (Da):20,884
Last modified:November 1, 1997 - v1
Checksum:i81A8BFBEF2DCB913
GO
Isoform Long (identifier: Q64387-2) [UniParc]FASTAAdd to basket
Also known as: N27K

The sequence of this isoform differs from the canonical sequence as follows:
     186-187: NV → IQVIPRTACVHSKTCRPGVRIPPSPRH

Note: No experimental confirmation available.
Show »
Length:212
Mass (Da):23,659
Checksum:i759A547E18A96E16
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80V → T in AAC52581 (PubMed:8670093).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001446186 – 187NV → IQVIPRTACVHSKTCRPGVR IPPSPRH in isoform Long. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82866 mRNA. Translation: BAA11614.1.
D50056 mRNA. Translation: BAA08774.1.
X97373 mRNA. Translation: CAA66042.1.
X97371, X97372 Genomic DNA. Translation: CAA66041.1.
U44027 mRNA. Translation: AAC52581.1.
D50055 mRNA. Translation: BAA08773.1.
CCDSiCCDS27213.1. [Q64387-1]
PIRiJC4502.
S70631.
RefSeqiNP_001192004.1. NM_001205075.1. [Q64387-2]
NP_035062.1. NM_010932.2. [Q64387-1]
XP_006518747.1. XM_006518684.2. [Q64387-2]
UniGeneiMm.16347.
Mm.486163.

Genome annotation databases

EnsembliENSMUST00000059339; ENSMUSP00000054210; ENSMUSG00000045731. [Q64387-1]
GeneIDi18155.
KEGGimmu:18155.
UCSCiuc007uji.2. mouse. [Q64387-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82866 mRNA. Translation: BAA11614.1.
D50056 mRNA. Translation: BAA08774.1.
X97373 mRNA. Translation: CAA66042.1.
X97371, X97372 Genomic DNA. Translation: CAA66041.1.
U44027 mRNA. Translation: AAC52581.1.
D50055 mRNA. Translation: BAA08773.1.
CCDSiCCDS27213.1. [Q64387-1]
PIRiJC4502.
S70631.
RefSeqiNP_001192004.1. NM_001205075.1. [Q64387-2]
NP_035062.1. NM_010932.2. [Q64387-1]
XP_006518747.1. XM_006518684.2. [Q64387-2]
UniGeneiMm.16347.
Mm.486163.

3D structure databases

ProteinModelPortaliQ64387.
SMRiQ64387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054210.

PTM databases

iPTMnetiQ64387.
PhosphoSitePlusiQ64387.

Proteomic databases

PaxDbiQ64387.
PRIDEiQ64387.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059339; ENSMUSP00000054210; ENSMUSG00000045731. [Q64387-1]
GeneIDi18155.
KEGGimmu:18155.
UCSCiuc007uji.2. mouse. [Q64387-1]

Organism-specific databases

CTDi5368.
MGIiMGI:105308. Pnoc.

Phylogenomic databases

eggNOGiENOG410IY0N. Eukaryota.
ENOG4111JDY. LUCA.
GeneTreeiENSGT00510000049153.
HOVERGENiHBG008227.
InParanoidiQ64387.
OMAiGCKSSAS.
OrthoDBiEOG091G0WQ7.
PhylomeDBiQ64387.
TreeFamiTF332620.

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiQ64387.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045731.
CleanExiMM_PNOC.
ExpressionAtlasiQ64387. baseline and differential.
GenevisibleiQ64387. MM.

Family and domain databases

InterProiIPR002367. Nociceptin.
IPR006024. Opioid_neupept.
[Graphical view]
PANTHERiPTHR11438. PTHR11438. 1 hit.
PfamiPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSiPR01028. OPIOIDPRCRSR.
PR01031. ORPHNNPRCRSR.
PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNOC_MOUSE
AccessioniPrimary (citable) accession number: Q64387
Secondary accession number(s): Q61105, Q61938
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.