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Protein

Sarcosine dehydrogenase, mitochondrial

Gene

Sardh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Sarcosine + H2O + electron-transfer flavoprotein = glycine + formaldehyde + reduced electron-transfer flavoprotein.

Cofactori

FAD2 PublicationsNote: Binds 1 FAD covalently per monomer.2 Publications

Pathwayi: sarcosine degradation

This protein is involved in step 1 of the subpathway that synthesizes formaldehyde and glycine from sarcosine.
Proteins known to be involved in this subpathway in this organism are:
  1. Sarcosine dehydrogenase, mitochondrial (Sardh)
This subpathway is part of the pathway sarcosine degradation, which is itself part of Amine and polyamine degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes formaldehyde and glycine from sarcosine, the pathway sarcosine degradation and in Amine and polyamine degradation.

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: UniProtKB
  • folic acid binding Source: RGD
  • oxidoreductase activity Source: UniProtKB
  • sarcosine dehydrogenase activity Source: RGD

GO - Biological processi

  • choline catabolic process Source: UniProtKB
  • glycine biosynthetic process Source: RGD
  • tetrahydrofolate interconversion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16116.
UniPathwayiUPA00292; UER00398.

Names & Taxonomyi

Protein namesi
Recommended name:
Sarcosine dehydrogenase, mitochondrial (EC:1.5.8.3)
Short name:
SarDH
Gene namesi
Name:SardhImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621125. Sardh.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222MitochondrionSequence analysisAdd
BLAST
Chaini23 – 919897Sarcosine dehydrogenase, mitochondrialPRO_0000010772Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei38 – 381N6-succinyllysineBy similarity
Modified residuei109 – 1091Tele-8alpha-FAD histidineBy similarity
Modified residuei174 – 1741N6-acetyllysine; alternateBy similarity
Modified residuei174 – 1741N6-succinyllysine; alternateBy similarity
Modified residuei378 – 3781N6-succinyllysineBy similarity
Modified residuei392 – 3921N6-succinyllysineBy similarity
Modified residuei535 – 5351N6-succinyllysineBy similarity
Modified residuei560 – 5601N6-acetyllysineBy similarity
Modified residuei776 – 7761N6-acetyllysineBy similarity
Modified residuei778 – 7781PhosphotyrosineBy similarity
Modified residuei803 – 8031N6-acetyllysine; alternateBy similarity
Modified residuei803 – 8031N6-succinyllysine; alternateBy similarity
Modified residuei885 – 8851N6-acetyllysine; alternateBy similarity
Modified residuei885 – 8851N6-succinyllysine; alternateBy similarity
Modified residuei905 – 9051N6-acetyllysine; alternateBy similarity
Modified residuei905 – 9051N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ64380.
PeptideAtlasiQ64380.
PRIDEiQ64380.

PTM databases

iPTMnetiQ64380.
PhosphoSiteiQ64380.

Expressioni

Tissue specificityi

High levels in liver, lung, kidney, thymus and oviduct, and intermediate levels in the prostate, seminal vesicle, cardiac muscle and neuro-intermediate pituitary lobe. Also expressed in pancreas and adrenal gland.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009555.

Structurei

3D structure databases

ProteinModelPortaliQ64380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2844. Eukaryota.
COG0404. LUCA.
HOGENOMiHOG000251716.
HOVERGENiHBG002326.
InParanoidiQ64380.
KOiK00314.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 3 hits.
3.50.50.60. 3 hits.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR032503. FAO_M.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF16350. FAO_M. 1 hit.
PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
SUPFAMiSSF101790. SSF101790. 1 hit.
SSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLSRVLRV AATCPRGRSA RNLGLQPLAR EARPTTEKSV PYQCTLKEEA
60 70 80 90 100
QGASVVPQGP SQPLPSTANV VVIGGGSLGC QTLYHLAKLG VGGVVLLERE
110 120 130 140 150
RLTSGTTWHT AGLLWQLRPS DVEVELLAHT RQVVSHDLEE ETGLHTGWIQ
160 170 180 190 200
NGGLFIASNQ QRLDEYKRLM SLGKAYGIES HVLSPAETKD LYPLMNVDDL
210 220 230 240 250
YGTLYVPRDG TMDPAGTCTT LTRAAVARGA QVIENCAVTG IRVRTDDFGV
260 270 280 290 300
RRVTAVETQH GSIQTPCVVN CAGVWASSVG RMAGVKVPLV AMHHAYVVTE
310 320 330 340 350
RIEGIQNMPN VRDHDASVYL RLQGDALSVG GYEANPIFWD EVSDKFAFGL
360 370 380 390 400
FDLDWDVFTQ HIEGAINRVP VLEKTGIKST VCGPESFTPD HKPLMGEAPE
410 420 430 440 450
LRGFFLGCGF NSAGMMLGGG CGQELAHWIV HGRPEKDMYS YDIRRFHHSL
460 470 480 490 500
TDHPRWIRER SHESYAKNYS VVFPHDEPLA GRNMRRDPLH EELLGQGCVF
510 520 530 540 550
QERQGWERPG WFNPQETAQV LDYDYYGAYG HQAHKDYAYS RLLGDEYTFD
560 570 580 590 600
FPPHHCVIQK ECLACRTAAA VFNMSYFGKF YLLGADARKA PDWLFSADVN
610 620 630 640 650
RPPGSTVYTC MLNQRGGTES DLTVSCLAPG AQASPLAPAF EGDGYYLAVG
660 670 680 690 700
GAVAQHNWSH INTVLQDQEF RCQLMDCSED LGMLSIQGPA SRDILQDVLD
710 720 730 740 750
ADLSNEAFPF STHQLVRAAG HLVRAIRLSF VGELGWELHV PQASCLPVYR
760 770 780 790 800
AVMAAGAKHG LVNAGYRAID SLSIEKGYRH WHADLRSDDS PLEAGLAFTC
810 820 830 840 850
KLKTSVPFLG REALEKQRAT GLRRRLVCLT VEEEVPMFGL EAIWRNGQVV
860 870 880 890 900
GHVRRADFGF TVNKTIAYGY IRDPSGGPVS LDFVKNGDYA LERMGVTYAA
910
QVHLKSPFDP DNKRVKGIY
Length:919
Mass (Da):101,440
Last modified:August 16, 2004 - v2
Checksum:iE49E54207BD1DBFB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 10211GGVVLLERERL → SGVGAAGERAM in AAB03674 (PubMed:9839943).CuratedAdd
BLAST
Sequence conflicti92 – 10211GGVVLLERERL → SGVGAAGERAM in AAB04667 (PubMed:9839943).CuratedAdd
BLAST
Sequence conflicti114 – 1152LW → GR AA sequence (PubMed:4055729).Curated
Sequence conflicti154 – 1541L → F in AAB03674 (PubMed:9839943).Curated
Sequence conflicti154 – 1541L → F in AAB04667 (PubMed:9839943).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067650 mRNA. Translation: AAD03414.1.
U62481 mRNA. Translation: AAB03674.1.
L79910 mRNA. Translation: AAB04667.1.
RefSeqiNP_446116.1. NM_053664.2.
UniGeneiRn.89832.

Genome annotation databases

GeneIDi114123.
KEGGirno:114123.
UCSCiRGD:621125. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067650 mRNA. Translation: AAD03414.1.
U62481 mRNA. Translation: AAB03674.1.
L79910 mRNA. Translation: AAB04667.1.
RefSeqiNP_446116.1. NM_053664.2.
UniGeneiRn.89832.

3D structure databases

ProteinModelPortaliQ64380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009555.

PTM databases

iPTMnetiQ64380.
PhosphoSiteiQ64380.

Proteomic databases

PaxDbiQ64380.
PeptideAtlasiQ64380.
PRIDEiQ64380.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114123.
KEGGirno:114123.
UCSCiRGD:621125. rat.

Organism-specific databases

CTDi1757.
RGDi621125. Sardh.

Phylogenomic databases

eggNOGiKOG2844. Eukaryota.
COG0404. LUCA.
HOGENOMiHOG000251716.
HOVERGENiHBG002326.
InParanoidiQ64380.
KOiK00314.

Enzyme and pathway databases

UniPathwayiUPA00292; UER00398.
BioCyciMetaCyc:MONOMER-16116.

Miscellaneous databases

PROiQ64380.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 3 hits.
3.50.50.60. 3 hits.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR032503. FAO_M.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF16350. FAO_M. 1 hit.
PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
SUPFAMiSSF101790. SSF101790. 1 hit.
SSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSARDH_RAT
AccessioniPrimary (citable) accession number: Q64380
Secondary accession number(s): O88499
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: August 16, 2004
Last modified: September 7, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.