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Protein

Sarcosine dehydrogenase, mitochondrial

Gene

Sardh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Sarcosine + H2O + electron-transfer flavoprotein = glycine + formaldehyde + reduced electron-transfer flavoprotein.

Cofactori

FAD2 PublicationsNote: Binds 1 FAD covalently per monomer.2 Publications

Pathwayi: sarcosine degradation

This protein is involved in step 1 of the subpathway that synthesizes formaldehyde and glycine from sarcosine.
Proteins known to be involved in this subpathway in this organism are:
  1. Sarcosine dehydrogenase, mitochondrial (Sardh)
This subpathway is part of the pathway sarcosine degradation, which is itself part of Amine and polyamine degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes formaldehyde and glycine from sarcosine, the pathway sarcosine degradation and in Amine and polyamine degradation.

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: UniProtKB
  • folic acid binding Source: RGD
  • oxidoreductase activity Source: UniProtKB
  • sarcosine dehydrogenase activity Source: RGD

GO - Biological processi

  • choline catabolic process Source: RGD
  • glycine biosynthetic process Source: RGD
  • sarcosine catabolic process Source: GO_Central
  • tetrahydrofolate interconversion Source: RGD

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16116
UniPathwayiUPA00292; UER00398

Names & Taxonomyi

Protein namesi
Recommended name:
Sarcosine dehydrogenase, mitochondrial (EC:1.5.8.3)
Short name:
SarDH
Gene namesi
Name:SardhImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621125 Sardh

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 22MitochondrionSequence analysisAdd BLAST22
ChainiPRO_000001077223 – 919Sarcosine dehydrogenase, mitochondrialAdd BLAST897

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei38N6-succinyllysineBy similarity1
Modified residuei109Tele-8alpha-FAD histidineBy similarity1
Modified residuei174N6-acetyllysine; alternateBy similarity1
Modified residuei174N6-succinyllysine; alternateBy similarity1
Modified residuei378N6-succinyllysineBy similarity1
Modified residuei392N6-succinyllysineBy similarity1
Modified residuei535N6-succinyllysineBy similarity1
Modified residuei560N6-acetyllysineBy similarity1
Modified residuei776N6-acetyllysineBy similarity1
Modified residuei778PhosphotyrosineBy similarity1
Modified residuei803N6-acetyllysine; alternateBy similarity1
Modified residuei803N6-succinyllysine; alternateBy similarity1
Modified residuei885N6-acetyllysine; alternateBy similarity1
Modified residuei885N6-succinyllysine; alternateBy similarity1
Modified residuei905N6-acetyllysine; alternateBy similarity1
Modified residuei905N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ64380
PeptideAtlasiQ64380
PRIDEiQ64380

PTM databases

iPTMnetiQ64380
PhosphoSitePlusiQ64380

Expressioni

Tissue specificityi

High levels in liver, lung, kidney, thymus and oviduct, and intermediate levels in the prostate, seminal vesicle, cardiac muscle and neuro-intermediate pituitary lobe. Also expressed in pancreas and adrenal gland.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009555

Structurei

3D structure databases

ProteinModelPortaliQ64380
SMRiQ64380
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2844 Eukaryota
COG0404 LUCA
HOGENOMiHOG000251716
HOVERGENiHBG002326
InParanoidiQ64380
KOiK00314

Family and domain databases

Gene3Di3.30.1360.120, 2 hits
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR006076 FAD-dep_OxRdtase
IPR036188 FAD/NAD-bd_sf
IPR032503 FAO_M
IPR013977 GCV_T_C
IPR006222 GCV_T_N
IPR029043 GcvT/YgfZ_C
IPR027266 TrmE/GcvT_dom1
PfamiView protein in Pfam
PF01266 DAO, 1 hit
PF16350 FAO_M, 1 hit
PF01571 GCV_T, 1 hit
PF08669 GCV_T_C, 1 hit
SUPFAMiSSF101790 SSF101790, 1 hit
SSF51905 SSF51905, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLSRVLRV AATCPRGRSA RNLGLQPLAR EARPTTEKSV PYQCTLKEEA
60 70 80 90 100
QGASVVPQGP SQPLPSTANV VVIGGGSLGC QTLYHLAKLG VGGVVLLERE
110 120 130 140 150
RLTSGTTWHT AGLLWQLRPS DVEVELLAHT RQVVSHDLEE ETGLHTGWIQ
160 170 180 190 200
NGGLFIASNQ QRLDEYKRLM SLGKAYGIES HVLSPAETKD LYPLMNVDDL
210 220 230 240 250
YGTLYVPRDG TMDPAGTCTT LTRAAVARGA QVIENCAVTG IRVRTDDFGV
260 270 280 290 300
RRVTAVETQH GSIQTPCVVN CAGVWASSVG RMAGVKVPLV AMHHAYVVTE
310 320 330 340 350
RIEGIQNMPN VRDHDASVYL RLQGDALSVG GYEANPIFWD EVSDKFAFGL
360 370 380 390 400
FDLDWDVFTQ HIEGAINRVP VLEKTGIKST VCGPESFTPD HKPLMGEAPE
410 420 430 440 450
LRGFFLGCGF NSAGMMLGGG CGQELAHWIV HGRPEKDMYS YDIRRFHHSL
460 470 480 490 500
TDHPRWIRER SHESYAKNYS VVFPHDEPLA GRNMRRDPLH EELLGQGCVF
510 520 530 540 550
QERQGWERPG WFNPQETAQV LDYDYYGAYG HQAHKDYAYS RLLGDEYTFD
560 570 580 590 600
FPPHHCVIQK ECLACRTAAA VFNMSYFGKF YLLGADARKA PDWLFSADVN
610 620 630 640 650
RPPGSTVYTC MLNQRGGTES DLTVSCLAPG AQASPLAPAF EGDGYYLAVG
660 670 680 690 700
GAVAQHNWSH INTVLQDQEF RCQLMDCSED LGMLSIQGPA SRDILQDVLD
710 720 730 740 750
ADLSNEAFPF STHQLVRAAG HLVRAIRLSF VGELGWELHV PQASCLPVYR
760 770 780 790 800
AVMAAGAKHG LVNAGYRAID SLSIEKGYRH WHADLRSDDS PLEAGLAFTC
810 820 830 840 850
KLKTSVPFLG REALEKQRAT GLRRRLVCLT VEEEVPMFGL EAIWRNGQVV
860 870 880 890 900
GHVRRADFGF TVNKTIAYGY IRDPSGGPVS LDFVKNGDYA LERMGVTYAA
910
QVHLKSPFDP DNKRVKGIY
Length:919
Mass (Da):101,440
Last modified:August 16, 2004 - v2
Checksum:iE49E54207BD1DBFB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92 – 102GGVVLLERERL → SGVGAAGERAM in AAB03674 (PubMed:9839943).CuratedAdd BLAST11
Sequence conflicti92 – 102GGVVLLERERL → SGVGAAGERAM in AAB04667 (PubMed:9839943).CuratedAdd BLAST11
Sequence conflicti114 – 115LW → GR AA sequence (PubMed:4055729).Curated2
Sequence conflicti154L → F in AAB03674 (PubMed:9839943).Curated1
Sequence conflicti154L → F in AAB04667 (PubMed:9839943).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067650 mRNA Translation: AAD03414.1
U62481 mRNA Translation: AAB03674.1
L79910 mRNA Translation: AAB04667.1
RefSeqiNP_446116.1, NM_053664.2
UniGeneiRn.89832

Genome annotation databases

GeneIDi114123
KEGGirno:114123
UCSCiRGD:621125 rat

Similar proteinsi

Entry informationi

Entry nameiSARDH_RAT
AccessioniPrimary (citable) accession number: Q64380
Secondary accession number(s): O88499
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: August 16, 2004
Last modified: March 28, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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