Reviewed, UniProtKB/Swiss-Prot Q64380 (SARDH_RAT)

Names and origin

Protein names

Recommended name:
    Sarcosine dehydrogenase, mitochondrial
      Short name=SarDH
    EC=1.5.99.1

Gene names

Name:

Sardh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	919 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Sarcosine + acceptor + H(2)O = glycine + formaldehyde + reduced acceptor.
Cofactor	Binds 1 FAD covalently per monomer.
Pathway	Amine and polyamine degradation; sarcosine degradation; formaldehyde and glycine from sarcosine: step 1/1.
Subcellular location	Mitochondrion matrix.
Tissue specificity	High levels in liver, lung, kidney, thymus and oviduct, and intermediate levels in the prostate, seminal vesicle, cardiac muscle and neuro-intermediate pituitary lobe. Also expressed in pancreas and adrenal gland.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	choline catabolic process Ref.1 Non-traceable author statement. Source: UniProtKB glycine catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	FAD binding Ref.1 Inferred from direct assay. Source: UniProtKB aminomethyltransferase activity Inferred from electronic annotation. Source: InterPro sarcosine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 919		Sarcosine dehydrogenase, mitochondrial		PRO_0000010772
Amino acid modifications
	Modified residue	109	1	Tele-8alpha-FAD histidine By similarity
	Modified residue	778	1	Phosphotyrosine By similarity
	Modified residue	885	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	92 – 102	11	GGVVLLERERL → SGVGAAGERAM in AAB03674 and AAB04667. Ref.1
	Sequence conflict	114 – 115	2	LW → GR AA sequence Ref.2
	Sequence conflict	154	1	L → F in AAB03674 and AAB04667. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q64380-1 [UniParc]. Last modified August 16, 2004. Version 2. Checksum: E49E54207BD1DBFB	FASTA	919	101,440
	10 20 30 40 50 60 MASLSRVLRV AATCPRGRSA RNLGLQPLAR EARPTTEKSV PYQCTLKEEA QGASVVPQGP 70 80 90 100 110 120 SQPLPSTANV VVIGGGSLGC QTLYHLAKLG VGGVVLLERE RLTSGTTWHT AGLLWQLRPS 130 140 150 160 170 180 DVEVELLAHT RQVVSHDLEE ETGLHTGWIQ NGGLFIASNQ QRLDEYKRLM SLGKAYGIES 190 200 210 220 230 240 HVLSPAETKD LYPLMNVDDL YGTLYVPRDG TMDPAGTCTT LTRAAVARGA QVIENCAVTG 250 260 270 280 290 300 IRVRTDDFGV RRVTAVETQH GSIQTPCVVN CAGVWASSVG RMAGVKVPLV AMHHAYVVTE 310 320 330 340 350 360 RIEGIQNMPN VRDHDASVYL RLQGDALSVG GYEANPIFWD EVSDKFAFGL FDLDWDVFTQ 370 380 390 400 410 420 HIEGAINRVP VLEKTGIKST VCGPESFTPD HKPLMGEAPE LRGFFLGCGF NSAGMMLGGG 430 440 450 460 470 480 CGQELAHWIV HGRPEKDMYS YDIRRFHHSL TDHPRWIRER SHESYAKNYS VVFPHDEPLA 490 500 510 520 530 540 GRNMRRDPLH EELLGQGCVF QERQGWERPG WFNPQETAQV LDYDYYGAYG HQAHKDYAYS 550 560 570 580 590 600 RLLGDEYTFD FPPHHCVIQK ECLACRTAAA VFNMSYFGKF YLLGADARKA PDWLFSADVN 610 620 630 640 650 660 RPPGSTVYTC MLNQRGGTES DLTVSCLAPG AQASPLAPAF EGDGYYLAVG GAVAQHNWSH 670 680 690 700 710 720 INTVLQDQEF RCQLMDCSED LGMLSIQGPA SRDILQDVLD ADLSNEAFPF STHQLVRAAG 730 740 750 760 770 780 HLVRAIRLSF VGELGWELHV PQASCLPVYR AVMAAGAKHG LVNAGYRAID SLSIEKGYRH 790 800 810 820 830 840 WHADLRSDDS PLEAGLAFTC KLKTSVPFLG REALEKQRAT GLRRRLVCLT VEEEVPMFGL 850 860 870 880 890 900 EAIWRNGQVV GHVRRADFGF TVNKTIAYGY IRDPSGGPVS LDFVKNGDYA LERMGVTYAA 910 QVHLKSPFDP DNKRVKGIY

References

[1]	"Molecular cloning and tissue distribution of rat sarcosine dehydrogenase." Bergeron F., Otto A., Blache P., Day R., Denoroy L., Brandsch R., Bataille D. Eur. J. Biochem. 257:556-561(1998) [PubMed: 9839943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 192-207, FAD-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Sprague-Dawley and Wistar. Tissue: Liver.
[2]	"The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria." Cook R.J., Misono K.S., Wagner C. J. Biol. Chem. 260:12998-13002(1985) [PubMed: 4055729] [Abstract] Cited for: PROTEIN SEQUENCE OF 102-115, FAD-BINDING. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF067650 mRNA. Translation: AAD03414.1. U62481 mRNA. Translation: AAB03674.1. L79910 mRNA. Translation: AAB04667.1.
RefSeq	NP_446116.1.
UniGene	Rn.89832
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000006916. Rattus norvegicus. [Contig view]
GeneID	114123.
KEGG	rno:114123.
Organism-specific databases
RGD	621125. Sardh.
Phylogenomic databases
HOVERGEN	Q64380.
Gene expression databases
ArrayExpress	Q64380.
GermOnline	ENSRNOG00000006916. Rattus norvegicus.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR013977. GCV_T_C. IPR006222. GCV_T_N. [Graphical view]
Pfam	PF01266. DAO. 1 hit. PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	618353.

Entry information

Entry name

SARDH_RAT

Accession

Primary (citable) accession number: Q64380
Secondary accession number(s): O88499

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)