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Reviewed, UniProtKB/Swiss-Prot Q64380 (SARDH_RAT)

Last modified November 25, 2008. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Sarcosine dehydrogenase, mitochondrial
      Short name=SarDH
    EC=1.5.99.1
Gene names
Name: Sardh
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length919 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

Sarcosine + acceptor + H(2)O = glycine + formaldehyde + reduced acceptor.

Cofactor

Binds 1 FAD covalently per monomer.

Pathway

Amine and polyamine degradation; sarcosine degradation; formaldehyde and glycine from sarcosine: step 1/1.

Subcellular location

Mitochondrion matrix.

Tissue specificity

High levels in liver, lung, kidney, thymus and oviduct, and intermediate levels in the prostate, seminal vesicle, cardiac muscle and neuro-intermediate pituitary lobe. Also expressed in pancreas and adrenal gland.

Sequence similarities

Belongs to the gcvT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 919Sarcosine dehydrogenase, mitochondrialPRO_0000010772

Amino acid modifications

Modified residue1091Tele-8alpha-FAD histidine By similarity
Modified residue7781Phosphotyrosine By similarity
Modified residue8851N6-acetyllysine By similarity

Experimental info

Sequence conflict92 – 10211GGVVLLERERL → SGVGAAGERAM in AAB03674 and AAB04667. Ref.1
Sequence conflict114 – 1152LW → GR AA sequence Ref.2
Sequence conflict1541L → F in AAB03674 and AAB04667. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q64380-1 [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: E49E54207BD1DBFB

FASTA919101,440
        10         20         30         40         50         60 
MASLSRVLRV AATCPRGRSA RNLGLQPLAR EARPTTEKSV PYQCTLKEEA QGASVVPQGP 

        70         80         90        100        110        120 
SQPLPSTANV VVIGGGSLGC QTLYHLAKLG VGGVVLLERE RLTSGTTWHT AGLLWQLRPS 

       130        140        150        160        170        180 
DVEVELLAHT RQVVSHDLEE ETGLHTGWIQ NGGLFIASNQ QRLDEYKRLM SLGKAYGIES 

       190        200        210        220        230        240 
HVLSPAETKD LYPLMNVDDL YGTLYVPRDG TMDPAGTCTT LTRAAVARGA QVIENCAVTG 

       250        260        270        280        290        300 
IRVRTDDFGV RRVTAVETQH GSIQTPCVVN CAGVWASSVG RMAGVKVPLV AMHHAYVVTE 

       310        320        330        340        350        360 
RIEGIQNMPN VRDHDASVYL RLQGDALSVG GYEANPIFWD EVSDKFAFGL FDLDWDVFTQ 

       370        380        390        400        410        420 
HIEGAINRVP VLEKTGIKST VCGPESFTPD HKPLMGEAPE LRGFFLGCGF NSAGMMLGGG 

       430        440        450        460        470        480 
CGQELAHWIV HGRPEKDMYS YDIRRFHHSL TDHPRWIRER SHESYAKNYS VVFPHDEPLA 

       490        500        510        520        530        540 
GRNMRRDPLH EELLGQGCVF QERQGWERPG WFNPQETAQV LDYDYYGAYG HQAHKDYAYS 

       550        560        570        580        590        600 
RLLGDEYTFD FPPHHCVIQK ECLACRTAAA VFNMSYFGKF YLLGADARKA PDWLFSADVN 

       610        620        630        640        650        660 
RPPGSTVYTC MLNQRGGTES DLTVSCLAPG AQASPLAPAF EGDGYYLAVG GAVAQHNWSH 

       670        680        690        700        710        720 
INTVLQDQEF RCQLMDCSED LGMLSIQGPA SRDILQDVLD ADLSNEAFPF STHQLVRAAG 

       730        740        750        760        770        780 
HLVRAIRLSF VGELGWELHV PQASCLPVYR AVMAAGAKHG LVNAGYRAID SLSIEKGYRH 

       790        800        810        820        830        840 
WHADLRSDDS PLEAGLAFTC KLKTSVPFLG REALEKQRAT GLRRRLVCLT VEEEVPMFGL 

       850        860        870        880        890        900 
EAIWRNGQVV GHVRRADFGF TVNKTIAYGY IRDPSGGPVS LDFVKNGDYA LERMGVTYAA 

       910 
QVHLKSPFDP DNKRVKGIY 

« Hide

References

[1]"Molecular cloning and tissue distribution of rat sarcosine dehydrogenase."
Bergeron F., Otto A., Blache P., Day R., Denoroy L., Brandsch R., Bataille D.
Eur. J. Biochem. 257:556-561(1998) [PubMed: 9839943] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 192-207, FAD-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: Sprague-Dawley and Wistar.
Tissue: Liver.
[2]"The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria."
Cook R.J., Misono K.S., Wagner C.
J. Biol. Chem. 260:12998-13002(1985) [PubMed: 4055729] [Abstract]
Cited for: PROTEIN SEQUENCE OF 102-115, FAD-BINDING.
Tissue: Liver.

Cross-references

Sequence databases

AF067650 mRNA. Translation: AAD03414.1.
U62481 mRNA. Translation: AAB03674.1.
L79910 mRNA. Translation: AAB04667.1.
RefSeqNP_446116.1.
UniGeneRn.89832

3D structure databases

ModBaseSearch...

Genome annotation databases

EnsemblENSRNOG00000006916. Rattus norvegicus. [Contig view]
GeneID114123.
KEGGrno:114123.

Organism-specific databases

RGD621125. Sardh.

Phylogenomic databases

HOVERGENQ64380.

Gene expression databases

ArrayExpressQ64380.
GermOnlineENSRNOG00000006916. Rattus norvegicus.

Family and domain databases

InterProIPR006076. FAD-dep_OxRdtase.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
[Graphical view]
PfamPF01266. DAO. 1 hit.
PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio618353.

Entry information

Entry nameSARDH_RAT
AccessionPrimary (citable) accession number: Q64380
Secondary accession number(s): O88499
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: August 16, 2004
Last modified: November 25, 2008
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents