Q64380 (SARDH_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 79.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Sarcosine dehydrogenase, mitochondrial Short name=SarDH EC=1.5.99.1 | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 919 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | Sarcosine + acceptor + H2O = glycine + formaldehyde + reduced acceptor. |
| Cofactor | |
| Pathway | |
| Subcellular location | |
| Tissue specificity | High levels in liver, lung, kidney, thymus and oviduct, and intermediate levels in the prostate, seminal vesicle, cardiac muscle and neuro-intermediate pituitary lobe. Also expressed in pancreas and adrenal gland. Ref.1 |
| Sequence similarities | Belongs to the GcvT family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | FAD Flavoprotein |
| Molecular function | Oxidoreductase |
| PTM | Acetylation Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | choline catabolic process Non-traceable author statement Ref.1. Source: UniProtKB glycine biosynthetic processTraceable author statement Ref.1. Source: RGD glycine catabolic processInferred from electronic annotation. Source: InterPro |
| Cellular component | mitochondrial matrix Inferred from direct assay Ref.1. Source: UniProtKB |
| Molecular function | aminomethyltransferase activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide bindingInferred from direct assay Ref.1. Source: UniProtKB sarcosine dehydrogenase activityTraceable author statement Ref.1. Source: RGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – ? | Mitochondrion Potential | |||||||
| Chain | ? – 919 | Sarcosine dehydrogenase, mitochondrial | PRO_0000010772 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 109 | 1 | Tele-8alpha-FAD histidine By similarity | ||||||
| Modified residue | 778 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 885 | 1 | N6-acetyllysine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 92 – 102 | 11 | GGVVLLERERL → SGVGAAGERAM in AAB03674. Ref.1 | ||||||
| Sequence conflict | 92 – 102 | 11 | GGVVLLERERL → SGVGAAGERAM in AAB04667. Ref.1 | ||||||
| Sequence conflict | 114 – 115 | 2 | LW → GR AA sequence Ref.2 | ||||||
| Sequence conflict | 154 | 1 | L → F in AAB03674. Ref.1 | ||||||
| Sequence conflict | 154 | 1 | L → F in AAB04667. Ref.1 | ||||||
Sequences
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References
| [1] | "Molecular cloning and tissue distribution of rat sarcosine dehydrogenase." Bergeron F., Otto A., Blache P., Day R., Denoroy L., Brandsch R., Bataille D. Eur. J. Biochem. 257:556-561(1998) [PubMed: 9839943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 192-207, FAD-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Sprague-Dawley and Wistar. Tissue: Liver. |
| [2] | "The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria." Cook R.J., Misono K.S., Wagner C. J. Biol. Chem. 260:12998-13002(1985) [PubMed: 4055729] [Abstract] Cited for: PROTEIN SEQUENCE OF 102-115, FAD-BINDING. Tissue: Liver. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF067650 mRNA. Translation: AAD03414.1. U62481 mRNA. Translation: AAB03674.1. L79910 mRNA. Translation: AAB04667.1. |
| IPI | IPI00209807. |
| RefSeq | NP_446116.1. NM_053664.1. |
| UniGene | Rn.89832. |
3D structure databases | |
| ProteinModelPortal | Q64380. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q64380. |
Proteomic databases | |
| PRIDE | Q64380. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 114123. |
| KEGG | rno:114123. |
Organism-specific databases | |
| CTD | 1757. |
| RGD | 621125. Sardh. |
Phylogenomic databases | |
| eggNOG | roNOG11048. |
| GeneTree | ENSGT00530000063120. |
| HOVERGEN | HBG002326. |
| InParanoid | Q64380. |
| OrthoDB | EOG4XKV66. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MONOMER-16116. |
Gene expression databases | |
| ArrayExpress | Q64380. |
| Genevestigator | Q64380. |
| GermOnline | ENSRNOG00000006916. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR006076. FAD-dep_OxRdtase. IPR013977. GCV_T_C. IPR006222. GCV_T_N. [Graphical view] |
| KO | K00314. |
| Pfam | PF01266. DAO. 1 hit. PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 618353. |
Entry information
| Entry name | SARDH_RAT | ||||||||
| Accession | Primary (citable) accession number: Q64380 Secondary accession number(s): O88499 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with