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Q64368

- DAZL_MOUSE

UniProt

Q64368 - DAZL_MOUSE

Protein

Deleted in azoospermia-like

Gene

Dazl

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts.2 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. mRNA 3'-UTR binding Source: MGI
    3. nucleotide binding Source: InterPro
    4. protein binding Source: UniProtKB
    5. translation activator activity Source: UniProtKB

    GO - Biological processi

    1. female meiosis II Source: MGI
    2. germ cell development Source: MGI
    3. multicellular organismal development Source: UniProtKB-KW
    4. oocyte maturation Source: MGI
    5. positive regulation of meiosis Source: MGI
    6. positive regulation of translational initiation Source: UniProtKB
    7. spermatogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Oogenesis, Spermatogenesis, Translation regulation

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deleted in azoospermia-like
    Alternative name(s):
    DAZ-like autosomal
    Deleted in azoospermia-like 1
    Gene namesi
    Name:Dazl
    Synonyms:Dazl1, Dazla
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1342328. Dazl.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. polysome Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi43 – 431F → D: Abolishes RNA-binding but not homodimerization; when associated with D-82; D-84 and D-87. 1 Publication
    Mutagenesisi82 – 821Y → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-84 and D-87. 1 Publication
    Mutagenesisi84 – 841F → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-82 and D-87. 1 Publication
    Mutagenesisi87 – 871F → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-82 and D-84. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 298298Deleted in azoospermia-likePRO_0000081561Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei276 – 2761Phosphotyrosine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ64368.
    PRIDEiQ64368.

    PTM databases

    PhosphoSiteiQ64368.

    Expressioni

    Tissue specificityi

    Expressed predominantly in testis with lower levels in ovary. In testis, it is expressed in pachytene spermatocytes and at lower level in type-B spermatogonia, preleptotene and zygotene spermatocytes. In ovary, it is expressed in maturing follicles. In embryonic and prepuberal ovary, it is expressed in the oocyte and follicular cells.1 Publication

    Developmental stagei

    In the testis, expression increases steadily after birth until the first spermatogonial cells appear, levels off as the first spermatogenic cells enter meiosis (10 days after birth) and remains at this level thereafter.

    Gene expression databases

    ArrayExpressiQ64368.
    BgeeiQ64368.
    CleanExiMM_DAZL.
    GenevestigatoriQ64368.

    Interactioni

    Subunit structurei

    Homodimer and heterodimer. Forms a heterodimer with DAZ. Interacts with BOLL, DAZAP1 and DAZAP2. Interacts with PUM2 By similarity. Multiple DAZL RRMs can bind to a single RNA containing multiple GUU triplets.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-2024439,EBI-2024439
    Dync1i1O884854EBI-2024439,EBI-492834
    Dynll1P6316812EBI-2024439,EBI-349121

    Protein-protein interaction databases

    IntActiQ64368. 6 interactions.
    MINTiMINT-133503.

    Structurei

    Secondary structure

    1
    298
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi36 – 4510
    Helixi53 – 608
    Helixi61 – 633
    Beta strandi66 – 738
    Beta strandi79 – 8911
    Helixi93 – 964
    Beta strandi102 – 1076
    Beta strandi109 – 1157

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2XS2X-ray1.35A32-132[»]
    2XS5X-ray1.60A/B32-117[»]
    2XS7X-ray1.45A32-117[»]
    2XSFX-ray1.70A35-118[»]
    ProteinModelPortaliQ64368.
    SMRiQ64368. Positions 32-117.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ64368.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 11576RRMPROSITE-ProRule annotationAdd
    BLAST
    Domaini167 – 19024DAZ-likeAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni80 – 13253HomodimerizationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi126 – 20782Pro-richAdd
    BLAST

    Domaini

    The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.By similarity

    Sequence similaritiesi

    Belongs to the RRM DAZ family.Curated
    Contains 1 DAZ-like domain.Curated
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG260394.
    GeneTreeiENSGT00530000063480.
    HOGENOMiHOG000246400.
    HOVERGENiHBG000573.
    InParanoidiQ64368.
    OMAiRSYVIPP.
    OrthoDBiEOG7BGHN4.
    PhylomeDBiQ64368.
    TreeFamiTF324396.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF00076. RRM_1. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64368-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSATTSEAPN SAVSREASTQ SSSATTSQGY VLPEGKIMPN TVFVGGIDVR    50
    MDETEIRSFF ARYGSVKEVK IITDRTGVSK GYGFVSFYND VDVQKIVESQ 100
    INFHGKKLKL GPAIRKQNLC TYHVQPRPLI FNPPPPPQFQ SVWSSPNAET 150
    YMQPPTMMNP ITQYVQAYPP YPSSPVQVIT GYQLPVYNYQ MPPQWPAGEQ 200
    RSYVIPPAYT TVNYHCSEVD PGADILPNEC SVHDAAPASG NGPQKKSVDR 250
    SIQTVVSCLF NPENRLRNSL VTQDDYFKDK RVHHFRRSRA VLKSDHLC 298
    Length:298
    Mass (Da):33,313
    Last modified:November 1, 1996 - v1
    Checksum:iFBF457BC388DB974
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X95724 mRNA. Translation: CAA65039.1.
    U38690 mRNA. Translation: AAB46608.1.
    U46694 mRNA. Translation: AAC52711.1.
    AK014854 mRNA. Translation: BAB29585.1.
    AK033183 mRNA. Translation: BAC28187.1.
    CCDSiCCDS37649.1.
    RefSeqiNP_001264792.1. NM_001277863.1.
    NP_034151.3. NM_010021.5.
    UniGeneiMm.280641.

    Genome annotation databases

    EnsembliENSMUST00000010736; ENSMUSP00000010736; ENSMUSG00000010592.
    GeneIDi13164.
    KEGGimmu:13164.
    UCSCiuc008cyu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X95724 mRNA. Translation: CAA65039.1 .
    U38690 mRNA. Translation: AAB46608.1 .
    U46694 mRNA. Translation: AAC52711.1 .
    AK014854 mRNA. Translation: BAB29585.1 .
    AK033183 mRNA. Translation: BAC28187.1 .
    CCDSi CCDS37649.1.
    RefSeqi NP_001264792.1. NM_001277863.1.
    NP_034151.3. NM_010021.5.
    UniGenei Mm.280641.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2XS2 X-ray 1.35 A 32-132 [» ]
    2XS5 X-ray 1.60 A/B 32-117 [» ]
    2XS7 X-ray 1.45 A 32-117 [» ]
    2XSF X-ray 1.70 A 35-118 [» ]
    ProteinModelPortali Q64368.
    SMRi Q64368. Positions 32-117.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q64368. 6 interactions.
    MINTi MINT-133503.

    PTM databases

    PhosphoSitei Q64368.

    Proteomic databases

    PaxDbi Q64368.
    PRIDEi Q64368.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000010736 ; ENSMUSP00000010736 ; ENSMUSG00000010592 .
    GeneIDi 13164.
    KEGGi mmu:13164.
    UCSCi uc008cyu.2. mouse.

    Organism-specific databases

    CTDi 1618.
    MGIi MGI:1342328. Dazl.

    Phylogenomic databases

    eggNOGi NOG260394.
    GeneTreei ENSGT00530000063480.
    HOGENOMi HOG000246400.
    HOVERGENi HBG000573.
    InParanoidi Q64368.
    OMAi RSYVIPP.
    OrthoDBi EOG7BGHN4.
    PhylomeDBi Q64368.
    TreeFami TF324396.

    Miscellaneous databases

    EvolutionaryTracei Q64368.
    NextBioi 283242.
    PROi Q64368.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64368.
    Bgeei Q64368.
    CleanExi MM_DAZL.
    Genevestigatori Q64368.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF00076. RRM_1. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a mouse homolog of the human DAZ (Deleted in Azoospermia) gene."
      Maiwald R., Luche R.M., Epstein C.J.
      Mamm. Genome 7:628-628(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: CD-1.
      Tissue: Testis.
    2. "A murine homologue of the human DAZ gene is autosomal and expressed only in male and female gonads."
      Cooke H.J., Lee M., Kerr S., Ruggiu M.
      Hum. Mol. Genet. 5:513-516(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    3. "Mouse autosomal homolog of DAZ, a candidate male sterility gene in humans, is expressed in male germ cells before and after puberty."
      Reijo R.A., Seligman J., Dinulos M.B., Jaffe T., Brown L.G., Disteche C.M., Page D.C.
      Genomics 35:346-352(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Testis.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    5. "The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis."
      Ruggiu M., Speed R., Taggart M., McKay S.J., Kilanowski F., Saunders P.T.K., Dorin J., Cooke H.J.
      Nature 389:73-77(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. "In vivo and in vitro analysis of homodimerisation activity of the mouse Dazl1 protein."
      Ruggiu M., Cooke H.J.
      Gene 252:119-126(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: HOMODIMERIZATION, INTERACTION WITH DAZ.
    7. "The RNA-binding specificity of the mouse Dazl protein."
      Venables J.P., Ruggiu M., Cooke H.J.
      Nucleic Acids Res. 29:2479-2483(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: RNA-BINDING, MUTAGENESIS OF PHE-43; TYR-82; PHE-84 AND PHE-87.
    8. "Phosphoproteome analysis of mouse liver using immobilized metal affinity purification and linear ion trap mass spectrometry."
      Jin W.-H., Dai J., Zhou H., Xia Q.-C., Zou H.-F., Zeng R.
      Rapid Commun. Mass Spectrom. 18:2169-2176(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-276.
    9. "Kinked beta-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL."
      Jenkins H.T., Malkova B., Edwards T.A.
      Proc. Natl. Acad. Sci. U.S.A. 108:18266-18271(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 32-132 ALONE AND IN COMPLEX WITH RNA, FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiDAZL_MOUSE
    AccessioniPrimary (citable) accession number: Q64368
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 17, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3