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Q64368

- DAZL_MOUSE

UniProt

Q64368 - DAZL_MOUSE

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Protein

Deleted in azoospermia-like

Gene

Dazl

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts.2 Publications

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. mRNA 3'-UTR binding Source: MGI
  3. nucleotide binding Source: InterPro
  4. translation activator activity Source: UniProtKB

GO - Biological processi

  1. female meiosis II Source: MGI
  2. germ cell development Source: MGI
  3. multicellular organismal development Source: UniProtKB-KW
  4. oocyte maturation Source: MGI
  5. positive regulation of meiosis Source: MGI
  6. positive regulation of translational initiation Source: UniProtKB
  7. spermatogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Oogenesis, Spermatogenesis, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Deleted in azoospermia-like
Alternative name(s):
DAZ-like autosomal
Deleted in azoospermia-like 1
Gene namesi
Name:Dazl
Synonyms:Dazl1, Dazla
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1342328. Dazl.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. polysome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi43 – 431F → D: Abolishes RNA-binding but not homodimerization; when associated with D-82; D-84 and D-87. 1 Publication
Mutagenesisi82 – 821Y → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-84 and D-87. 1 Publication
Mutagenesisi84 – 841F → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-82 and D-87. 1 Publication
Mutagenesisi87 – 871F → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-82 and D-84. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Deleted in azoospermia-likePRO_0000081561Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei276 – 2761Phosphotyrosine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64368.
PRIDEiQ64368.

PTM databases

PhosphoSiteiQ64368.

Expressioni

Tissue specificityi

Expressed predominantly in testis with lower levels in ovary. In testis, it is expressed in pachytene spermatocytes and at lower level in type-B spermatogonia, preleptotene and zygotene spermatocytes. In ovary, it is expressed in maturing follicles. In embryonic and prepuberal ovary, it is expressed in the oocyte and follicular cells.1 Publication

Developmental stagei

In the testis, expression increases steadily after birth until the first spermatogonial cells appear, levels off as the first spermatogenic cells enter meiosis (10 days after birth) and remains at this level thereafter.

Gene expression databases

BgeeiQ64368.
CleanExiMM_DAZL.
ExpressionAtlasiQ64368. baseline and differential.
GenevestigatoriQ64368.

Interactioni

Subunit structurei

Homodimer and heterodimer. Forms a heterodimer with DAZ. Interacts with BOLL, DAZAP1 and DAZAP2. Interacts with PUM2 (By similarity). Multiple DAZL RRMs can bind to a single RNA containing multiple GUU triplets.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-2024439,EBI-2024439
Dync1i1O884854EBI-2024439,EBI-492834
Dynll1P6316812EBI-2024439,EBI-349121

Protein-protein interaction databases

IntActiQ64368. 6 interactions.
MINTiMINT-133503.

Structurei

Secondary structure

1
298
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 4510Combined sources
Helixi53 – 608Combined sources
Helixi61 – 633Combined sources
Beta strandi66 – 738Combined sources
Beta strandi79 – 8911Combined sources
Helixi93 – 964Combined sources
Beta strandi102 – 1076Combined sources
Beta strandi109 – 1157Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XS2X-ray1.35A32-132[»]
2XS5X-ray1.60A/B32-117[»]
2XS7X-ray1.45A32-117[»]
2XSFX-ray1.70A35-118[»]
ProteinModelPortaliQ64368.
SMRiQ64368. Positions 32-117.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ64368.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 11576RRMPROSITE-ProRule annotationAdd
BLAST
Domaini167 – 19024DAZ-likeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 13253HomodimerizationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi126 – 20782Pro-richAdd
BLAST

Domaini

The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.By similarity

Sequence similaritiesi

Belongs to the RRM DAZ family.Curated
Contains 1 DAZ-like domain.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG260394.
GeneTreeiENSGT00530000063480.
HOGENOMiHOG000246400.
HOVERGENiHBG000573.
InParanoidiQ64368.
OMAiRSYVIPP.
OrthoDBiEOG7BGHN4.
PhylomeDBiQ64368.
TreeFamiTF324396.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64368-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSATTSEAPN SAVSREASTQ SSSATTSQGY VLPEGKIMPN TVFVGGIDVR
60 70 80 90 100
MDETEIRSFF ARYGSVKEVK IITDRTGVSK GYGFVSFYND VDVQKIVESQ
110 120 130 140 150
INFHGKKLKL GPAIRKQNLC TYHVQPRPLI FNPPPPPQFQ SVWSSPNAET
160 170 180 190 200
YMQPPTMMNP ITQYVQAYPP YPSSPVQVIT GYQLPVYNYQ MPPQWPAGEQ
210 220 230 240 250
RSYVIPPAYT TVNYHCSEVD PGADILPNEC SVHDAAPASG NGPQKKSVDR
260 270 280 290
SIQTVVSCLF NPENRLRNSL VTQDDYFKDK RVHHFRRSRA VLKSDHLC
Length:298
Mass (Da):33,313
Last modified:November 1, 1996 - v1
Checksum:iFBF457BC388DB974
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95724 mRNA. Translation: CAA65039.1.
U38690 mRNA. Translation: AAB46608.1.
U46694 mRNA. Translation: AAC52711.1.
AK014854 mRNA. Translation: BAB29585.1.
AK033183 mRNA. Translation: BAC28187.1.
CCDSiCCDS37649.1.
RefSeqiNP_001264792.1. NM_001277863.1.
NP_034151.3. NM_010021.5.
UniGeneiMm.280641.

Genome annotation databases

EnsembliENSMUST00000010736; ENSMUSP00000010736; ENSMUSG00000010592.
GeneIDi13164.
KEGGimmu:13164.
UCSCiuc008cyu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95724 mRNA. Translation: CAA65039.1 .
U38690 mRNA. Translation: AAB46608.1 .
U46694 mRNA. Translation: AAC52711.1 .
AK014854 mRNA. Translation: BAB29585.1 .
AK033183 mRNA. Translation: BAC28187.1 .
CCDSi CCDS37649.1.
RefSeqi NP_001264792.1. NM_001277863.1.
NP_034151.3. NM_010021.5.
UniGenei Mm.280641.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2XS2 X-ray 1.35 A 32-132 [» ]
2XS5 X-ray 1.60 A/B 32-117 [» ]
2XS7 X-ray 1.45 A 32-117 [» ]
2XSF X-ray 1.70 A 35-118 [» ]
ProteinModelPortali Q64368.
SMRi Q64368. Positions 32-117.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q64368. 6 interactions.
MINTi MINT-133503.

PTM databases

PhosphoSitei Q64368.

Proteomic databases

PaxDbi Q64368.
PRIDEi Q64368.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000010736 ; ENSMUSP00000010736 ; ENSMUSG00000010592 .
GeneIDi 13164.
KEGGi mmu:13164.
UCSCi uc008cyu.2. mouse.

Organism-specific databases

CTDi 1618.
MGIi MGI:1342328. Dazl.

Phylogenomic databases

eggNOGi NOG260394.
GeneTreei ENSGT00530000063480.
HOGENOMi HOG000246400.
HOVERGENi HBG000573.
InParanoidi Q64368.
OMAi RSYVIPP.
OrthoDBi EOG7BGHN4.
PhylomeDBi Q64368.
TreeFami TF324396.

Miscellaneous databases

EvolutionaryTracei Q64368.
NextBioi 283242.
PROi Q64368.
SOURCEi Search...

Gene expression databases

Bgeei Q64368.
CleanExi MM_DAZL.
ExpressionAtlasi Q64368. baseline and differential.
Genevestigatori Q64368.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
Pfami PF00076. RRM_1. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a mouse homolog of the human DAZ (Deleted in Azoospermia) gene."
    Maiwald R., Luche R.M., Epstein C.J.
    Mamm. Genome 7:628-628(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
    Tissue: Testis.
  2. "A murine homologue of the human DAZ gene is autosomal and expressed only in male and female gonads."
    Cooke H.J., Lee M., Kerr S., Ruggiu M.
    Hum. Mol. Genet. 5:513-516(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  3. "Mouse autosomal homolog of DAZ, a candidate male sterility gene in humans, is expressed in male germ cells before and after puberty."
    Reijo R.A., Seligman J., Dinulos M.B., Jaffe T., Brown L.G., Disteche C.M., Page D.C.
    Genomics 35:346-352(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Testis.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  5. "The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis."
    Ruggiu M., Speed R., Taggart M., McKay S.J., Kilanowski F., Saunders P.T.K., Dorin J., Cooke H.J.
    Nature 389:73-77(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "In vivo and in vitro analysis of homodimerisation activity of the mouse Dazl1 protein."
    Ruggiu M., Cooke H.J.
    Gene 252:119-126(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: HOMODIMERIZATION, INTERACTION WITH DAZ.
  7. "The RNA-binding specificity of the mouse Dazl protein."
    Venables J.P., Ruggiu M., Cooke H.J.
    Nucleic Acids Res. 29:2479-2483(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING, MUTAGENESIS OF PHE-43; TYR-82; PHE-84 AND PHE-87.
  8. "Phosphoproteome analysis of mouse liver using immobilized metal affinity purification and linear ion trap mass spectrometry."
    Jin W.-H., Dai J., Zhou H., Xia Q.-C., Zou H.-F., Zeng R.
    Rapid Commun. Mass Spectrom. 18:2169-2176(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-276.
  9. "Kinked beta-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL."
    Jenkins H.T., Malkova B., Edwards T.A.
    Proc. Natl. Acad. Sci. U.S.A. 108:18266-18271(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 32-132 ALONE AND IN COMPLEX WITH RNA, FUNCTION, SUBUNIT.

Entry informationi

Entry nameiDAZL_MOUSE
AccessioniPrimary (citable) accession number: Q64368
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3