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Q64368 (DAZL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deleted in azoospermia-like
Alternative name(s):
DAZ-like autosomal
Deleted in azoospermia-like 1
Gene names
Name:Dazl
Synonyms:Dazl1, Dazla
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts. Ref.5 Ref.9

Subunit structure

Homodimer and heterodimer. Forms a heterodimer with DAZ. Interacts with BOLL, DAZAP1 and DAZAP2. Interacts with PUM2 By similarity. Multiple DAZL RRMs can bind to a single RNA containing multiple GUU triplets. Ref.6 Ref.9

Subcellular location

Cytoplasm Ref.5.

Tissue specificity

Expressed predominantly in testis with lower levels in ovary. In testis, it is expressed in pachytene spermatocytes and at lower level in type-B spermatogonia, preleptotene and zygotene spermatocytes. In ovary, it is expressed in maturing follicles. In embryonic and prepuberal ovary, it is expressed in the oocyte and follicular cells. Ref.5

Developmental stage

In the testis, expression increases steadily after birth until the first spermatogonial cells appear, levels off as the first spermatogenic cells enter meiosis (10 days after birth) and remains at this level thereafter.

Domain

The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2 By similarity.

Sequence similarities

Belongs to the RRM DAZ family.

Contains 1 DAZ-like domain.

Contains 1 RRM (RNA recognition motif) domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 298298Deleted in azoospermia-like
PRO_0000081561

Regions

Domain40 – 11576RRM
Domain167 – 19024DAZ-like
Region80 – 13253Homodimerization
Compositional bias126 – 20782Pro-rich

Amino acid modifications

Modified residue2761Phosphotyrosine Ref.8

Experimental info

Mutagenesis431F → D: Abolishes RNA-binding but not homodimerization; when associated with D-82; D-84 and D-87. Ref.7
Mutagenesis821Y → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-84 and D-87. Ref.7
Mutagenesis841F → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-82 and D-87. Ref.7
Mutagenesis871F → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-82 and D-84. Ref.7

Secondary structure

................ 298
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q64368 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: FBF457BC388DB974

FASTA29833,313
        10         20         30         40         50         60 
MSATTSEAPN SAVSREASTQ SSSATTSQGY VLPEGKIMPN TVFVGGIDVR MDETEIRSFF 

        70         80         90        100        110        120 
ARYGSVKEVK IITDRTGVSK GYGFVSFYND VDVQKIVESQ INFHGKKLKL GPAIRKQNLC 

       130        140        150        160        170        180 
TYHVQPRPLI FNPPPPPQFQ SVWSSPNAET YMQPPTMMNP ITQYVQAYPP YPSSPVQVIT 

       190        200        210        220        230        240 
GYQLPVYNYQ MPPQWPAGEQ RSYVIPPAYT TVNYHCSEVD PGADILPNEC SVHDAAPASG 

       250        260        270        280        290 
NGPQKKSVDR SIQTVVSCLF NPENRLRNSL VTQDDYFKDK RVHHFRRSRA VLKSDHLC 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of a mouse homolog of the human DAZ (Deleted in Azoospermia) gene."
Maiwald R., Luche R.M., Epstein C.J.
Mamm. Genome 7:628-628(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CD-1.
Tissue: Testis.
[2]"A murine homologue of the human DAZ gene is autosomal and expressed only in male and female gonads."
Cooke H.J., Lee M., Kerr S., Ruggiu M.
Hum. Mol. Genet. 5:513-516(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[3]"Mouse autosomal homolog of DAZ, a candidate male sterility gene in humans, is expressed in male germ cells before and after puberty."
Reijo R.A., Seligman J., Dinulos M.B., Jaffe T., Brown L.G., Disteche C.M., Page D.C.
Genomics 35:346-352(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Testis.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Testis.
[5]"The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis."
Ruggiu M., Speed R., Taggart M., McKay S.J., Kilanowski F., Saunders P.T.K., Dorin J., Cooke H.J.
Nature 389:73-77(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]"In vivo and in vitro analysis of homodimerisation activity of the mouse Dazl1 protein."
Ruggiu M., Cooke H.J.
Gene 252:119-126(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: HOMODIMERIZATION, INTERACTION WITH DAZ.
[7]"The RNA-binding specificity of the mouse Dazl protein."
Venables J.P., Ruggiu M., Cooke H.J.
Nucleic Acids Res. 29:2479-2483(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: RNA-BINDING, MUTAGENESIS OF PHE-43; TYR-82; PHE-84 AND PHE-87.
[8]"Phosphoproteome analysis of mouse liver using immobilized metal affinity purification and linear ion trap mass spectrometry."
Jin W.-H., Dai J., Zhou H., Xia Q.-C., Zou H.-F., Zeng R.
Rapid Commun. Mass Spectrom. 18:2169-2176(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT TYR-276.
[9]"Kinked beta-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL."
Jenkins H.T., Malkova B., Edwards T.A.
Proc. Natl. Acad. Sci. U.S.A. 108:18266-18271(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 32-132 ALONE AND IN COMPLEX WITH RNA, FUNCTION, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X95724 mRNA. Translation: CAA65039.1.
U38690 mRNA. Translation: AAB46608.1.
U46694 mRNA. Translation: AAC52711.1.
AK014854 mRNA. Translation: BAB29585.1.
AK033183 mRNA. Translation: BAC28187.1.
RefSeqNP_001264792.1. NM_001277863.1.
NP_034151.3. NM_010021.5.
UniGeneMm.280641.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2XS2X-ray1.35A32-132[»]
2XS5X-ray1.60A/B32-117[»]
2XS7X-ray1.45A32-117[»]
2XSFX-ray1.70A35-118[»]
ProteinModelPortalQ64368.
SMRQ64368. Positions 32-117.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ64368. 6 interactions.
MINTMINT-133503.

PTM databases

PhosphoSiteQ64368.

Proteomic databases

PaxDbQ64368.
PRIDEQ64368.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000010736; ENSMUSP00000010736; ENSMUSG00000010592.
GeneID13164.
KEGGmmu:13164.
UCSCuc008cyu.2. mouse.

Organism-specific databases

CTD1618.
MGIMGI:1342328. Dazl.

Phylogenomic databases

eggNOGNOG260394.
GeneTreeENSGT00530000063480.
HOGENOMHOG000246400.
HOVERGENHBG000573.
InParanoidQ64368.
OMARSYVIPP.
OrthoDBEOG7BGHN4.
PhylomeDBQ64368.
TreeFamTF324396.

Gene expression databases

ArrayExpressQ64368.
BgeeQ64368.
CleanExMM_DAZL.
GenevestigatorQ64368.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ64368.
NextBio283242.
PROQ64368.
SOURCESearch...

Entry information

Entry nameDAZL_MOUSE
AccessionPrimary (citable) accession number: Q64368
Entry history
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot