Q64368 (DAZL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deleted in azoospermia-like

Alternative name(s):
DAZ-like autosomal
Deleted in azoospermia-like 1

Gene names

Name:	Dazl
Synonyms:	Dazl1, Dazla

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts. Ref.5 Ref.9
Subunit structure	Homodimer and heterodimer. Forms a heterodimer with DAZ. Interacts with BOLL, DAZAP1 and DAZAP2. Interacts with PUM2 By similarity. Multiple DAZL RRMs can bind to a single RNA containing multiple GUU triplets. Ref.6 Ref.9
Subcellular location	Cytoplasm Ref.5.
Tissue specificity	Expressed predominantly in testis with lower levels in ovary. In testis, it is expressed in pachytene spermatocytes and at lower level in type-B spermatogonia, preleptotene and zygotene spermatocytes. In ovary, it is expressed in maturing follicles. In embryonic and prepuberal ovary, it is expressed in the oocyte and follicular cells. Ref.5
Developmental stage	In the testis, expression increases steadily after birth until the first spermatogonial cells appear, levels off as the first spermatogenic cells enter meiosis (10 days after birth) and remains at this level thereafter.
Domain	The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2 By similarity.
Sequence similarities	Belongs to the RRM DAZ family. Contains 1 DAZ-like domain. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	Differentiation Oogenesis Spermatogenesis Translation regulation
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	germ cell development Inferred from mutant phenotype PubMed 14611631PubMed 16310179Ref.5. Source: MGI multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW oogenesis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of meiosis Inferred from mutant phenotype PubMed 14611631. Source: MGI positive regulation of translational initiation Inferred from direct assay PubMed 16001084. Source: UniProtKB spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay Ref.5. Source: MGI polysome Inferred from direct assay PubMed 11604102. Source: MGI
Molecular_function	mRNA 3'-UTR binding Inferred from direct assay PubMed 11804965. Source: MGI nucleotide binding Inferred from electronic annotation. Source: InterPro translation activator activity Inferred from direct assay PubMed 16001084. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-2024439,EBI-2024439

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 298

298

Deleted in azoospermia-like

PRO_0000081561

Regions

Domain

40 – 115

76

RRM

Domain

167 – 190

24

DAZ-like

Region

80 – 132

53

Homodimerization

Compositional bias

126 – 207

82

Pro-rich

Amino acid modifications

Modified residue

276

1

Phosphotyrosine Ref.8

Experimental info

Mutagenesis

43

1

F → D: Abolishes RNA-binding but not homodimerization; when associated with D-82; D-84 and D-87. Ref.7

Mutagenesis

82

1

Y → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-84 and D-87. Ref.7

Mutagenesis

84

1

F → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-82 and D-87. Ref.7

Mutagenesis

87

1

F → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-82 and D-84. Ref.7

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

298

Helix Strand Turn

Details...

Beta strand

36 – 45

10

Helix

53 – 60

8

Helix

61 – 63

3

Beta strand

66 – 73

8

Beta strand

79 – 89

11

Helix

93 – 96

4

Beta strand

102 – 107

6

Beta strand

109 – 115

7

Sequences

	Sequence		Length	Mass (Da)
	Q64368 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FBF457BC388DB974	FASTA	298	33,313
	10 20 30 40 50 60 MSATTSEAPN SAVSREASTQ SSSATTSQGY VLPEGKIMPN TVFVGGIDVR MDETEIRSFF 70 80 90 100 110 120 ARYGSVKEVK IITDRTGVSK GYGFVSFYND VDVQKIVESQ INFHGKKLKL GPAIRKQNLC 130 140 150 160 170 180 TYHVQPRPLI FNPPPPPQFQ SVWSSPNAET YMQPPTMMNP ITQYVQAYPP YPSSPVQVIT 190 200 210 220 230 240 GYQLPVYNYQ MPPQWPAGEQ RSYVIPPAYT TVNYHCSEVD PGADILPNEC SVHDAAPASG 250 260 270 280 290 NGPQKKSVDR SIQTVVSCLF NPENRLRNSL VTQDDYFKDK RVHHFRRSRA VLKSDHLC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a mouse homolog of the human DAZ (Deleted in Azoospermia) gene." Maiwald R., Luche R.M., Epstein C.J. Mamm. Genome 7:628-628(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1. Tissue: Testis.
[2]	"A murine homologue of the human DAZ gene is autosomal and expressed only in male and female gonads." Cooke H.J., Lee M., Kerr S., Ruggiu M. Hum. Mol. Genet. 5:513-516(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[3]	"Mouse autosomal homolog of DAZ, a candidate male sterility gene in humans, is expressed in male germ cells before and after puberty." Reijo R.A., Seligman J., Dinulos M.B., Jaffe T., Brown L.G., Disteche C.M., Page D.C. Genomics 35:346-352(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Testis.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Testis.
[5]	"The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis." Ruggiu M., Speed R., Taggart M., McKay S.J., Kilanowski F., Saunders P.T.K., Dorin J., Cooke H.J. Nature 389:73-77(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"In vivo and in vitro analysis of homodimerisation activity of the mouse Dazl1 protein." Ruggiu M., Cooke H.J. Gene 252:119-126(2000) [PubMed] [Europe PMC] [Abstract] Cited for: HOMODIMERIZATION, INTERACTION WITH DAZ.
[7]	"The RNA-binding specificity of the mouse Dazl protein." Venables J.P., Ruggiu M., Cooke H.J. Nucleic Acids Res. 29:2479-2483(2001) [PubMed] [Europe PMC] [Abstract] Cited for: RNA-BINDING, MUTAGENESIS OF PHE-43; TYR-82; PHE-84 AND PHE-87.
[8]	"Phosphoproteome analysis of mouse liver using immobilized metal affinity purification and linear ion trap mass spectrometry." Jin W.-H., Dai J., Zhou H., Xia Q.-C., Zou H.-F., Zeng R. Rapid Commun. Mass Spectrom. 18:2169-2176(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT TYR-276.
[9]	"Kinked beta-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL." Jenkins H.T., Malkova B., Edwards T.A. Proc. Natl. Acad. Sci. U.S.A. 108:18266-18271(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 32-132 ALONE AND IN COMPLEX WITH RNA, FUNCTION, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X95724 mRNA. Translation: CAA65039.1.
U38690 mRNA. Translation: AAB46608.1.
U46694 mRNA. Translation: AAC52711.1.
AK014854 mRNA. Translation: BAB29585.1.
AK033183 mRNA. Translation: BAC28187.1.

IPI

IPI00262067.

RefSeq

NP_034151.3. NM_010021.4.

UniGene

Mm.280641.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2XS2	X-ray	1.35	A	32-132	[»]
2XS5	X-ray	1.60	A/B	32-117	[»]
2XS7	X-ray	1.45	A	32-117	[»]
2XSF	X-ray	1.70	A	35-118	[»]

ProteinModelPortal

Q64368.

SMR

Q64368. Positions 32-117.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q64368. 1 interaction.

MINT

MINT-133503.

PTM databases

PhosphoSite

Q64368.

Proteomic databases

PaxDb

Q64368.

PRIDE

Q64368.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000010736; ENSMUSP00000010736; ENSMUSG00000010592.

GeneID

13164.

KEGG

mmu:13164.

UCSC

uc008cyv.2. mouse.

Organism-specific databases

CTD

1618.

MGI

MGI:1342328. Dazl.

Phylogenomic databases

eggNOG

NOG260394.

GeneTree

ENSGT00530000063480.

HOGENOM

HOG000246400.

HOVERGEN

HBG000573.

InParanoid

Q64368.

OMA

YVIPPAY.

OrthoDB

EOG4T783S.

Gene expression databases

ArrayExpress

Q64368.

Bgee

Q64368.

CleanEx

MM_DAZL.

Genevestigator

Q64368.

GermOnline

ENSMUSG00000010592. Mus musculus.

Family and domain databases

Gene3D

3.30.70.330. 1 hit.

InterPro

IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]

Pfam

PF00076. RRM_1. 1 hit.
[Graphical view]

SMART

SM00360. RRM. 1 hit.
[Graphical view]

PROSITE

PS50102. RRM. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q64368.

NextBio

283242.

SOURCE

Search...

Entry information

Entry name

DAZL_MOUSE

Accession

Primary (citable) accession number: Q64368

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program