Cyclic nucleotide-gated cation channel alpha-4

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Q64359 (CNGA4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cyclic nucleotide-gated cation channel alpha-4

Alternative name(s):
Cyclic nucleotide-gated channel alpha-4
Short name=CNG channel alpha-4
Short name=CNG-4
Short name=CNG4
Cyclic nucleotide-gated olfactory channel subunit OCNC2

Gene names

Name:	Cnga4
Synonyms:	Cgn2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Second messenger, cAMP, causes the opening of cation-selective cyclic nucleotide-gated (CNG) channels and depolarization of the neuron (olfactory sensory neurons, OSNs). CNGA4 is the modulatory subunit of this channel which is known to play a central role in the transduction of odorant signals and subsequent adaptation. By accelerating the calcium-mediated negative feedback in olfactory signaling it allows rapid adaptation to odor stimulation and extends its range of odor detection By similarity.
Enzyme regulation	Calcium-calmodulin exerts its inhibitory effect in cAMP sensitivity by binding to IQ-like motif of CNGA4 and preferably binds to the channel in the closed state. Inhibition by PIP3 of the CNG channel probably occurs via CGNA2 binding. Ref.5
Subunit structure	Heterotetramer composed of two subunits of CNGA2, one of CNGA4 and one of CNGB1b. The complex forms the cyclic nucleotide-gated (CNG) channel of olfactory sensory neurons. Ref.6
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Olfactory neurons.
Sequence similarities	Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGA4 subfamily. [View classification] Contains 1 cyclic nucleotide-binding domain.

Ontologies

Keywords
Biological process	Ion transport Olfaction Sensory transduction Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Nucleotide-binding cAMP cAMP-binding
Molecular function	Ionic channel Ligand-gated ion channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of smell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular cyclic nucleotide activated cation channel complex Inferred from direct assay Ref.2. Source: RGD
Molecular function	cAMP binding Inferred from electronic annotation. Source: UniProtKB-KW intracellular cyclic nucleotide activated cation channel activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 575

575

Cyclic nucleotide-gated cation channel alpha-4

PRO_0000219322

Regions

Topological domain

1 – 33

Cytoplasmic Potential

Transmembrane

34 – 54

Helical; Name=H1; Potential

Topological domain

55 – 65

Extracellular Potential

Transmembrane

66 – 86

Helical; Name=H2; Potential

Topological domain

87 – 112

Cytoplasmic Potential

Transmembrane

113 – 133

Helical; Name=H3; Potential

Topological domain

134 – 168

Extracellular Potential

Transmembrane

169 – 189

Helical; Name=H4; Potential

Topological domain

190 – 218

Cytoplasmic Potential

Transmembrane

219 – 239

Helical; Name=H5; Potential

Topological domain

240 – 244

Extracellular Potential

Transmembrane

245 – 265

Helical; Name=H6; Potential

Topological domain

266 – 575

310

Cytoplasmic Potential

Nucleotide binding

348 – 471

124

cNMP

Motif

292 – 302

IQ-type By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q64359 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: FCF01C563214F575
Show »

FASTA

575

65,675

        10         20         30         40         50         60 
MSQDGKVKTT ESTPPAPTKA RKWLPVLDPS GDYYYWWLNT MVFPIMYNLI IVVCRACFPD 

        70         80         90        100        110        120 
LQHSYLVAWF VLDYTSDLLY LLDIGVRFHT GFLEQGILVV DKGMIASRYV RTWSFLLDLA 

       130        140        150        160        170        180 
SLVPTDAAYV QLGPHIPTLR LNRFLRVPRL FEAFDRTETR TAYPNAFRIA KLMLYIFVVI 

       190        200        210        220        230        240 
HWNSCLYFAL SRYLGFGRDA WVYPDPAQPG FERLRRQYLY SFYFSTLILT TVGDTPLPDR 

       250        260        270        280        290        300 
EEEYLFMVGD FLLAVMGFAT IMGSMSSVIY NMNTADAAFY PDHALVKKYM KLQHVNKRLE 

       310        320        330        340        350        360 
RRVIDWYQHL QINKKMTNEV AILQHLPERL RAEVAVSVHL STLSRVQIFQ NCEASLLEEL 

       370        380        390        400        410        420 
VLKLQPQTYS PGEYVCRKGD IGREMYIIRE GQLAVVADDG VTQYAVLGAG LYFGEISIIN 

       430        440        450        460        470        480 
IKGNMSGNRR TANIKSLGYS DLFCLSKEDL REVLSEYPQA QAVMEEKGRE ILLKMNKLDV 

       490        500        510        520        530        540 
NAEAAEIALQ EATESRLKGL DQQLDDLQTK FARLLAELES SALKIAYRIE RLEWQTREWP 

       550        560        570 
MPEDMGEADD EAEPGEGTSK DGEGKAGQAG PSGIE

« Hide

References

[1]	"Heteromeric olfactory cyclic nucleotide-gated channels: a subunit that confers increased sensitivity to cAMP." Bradley J., Li J., Davidson N., Lester H.A., Zinn K. Proc. Natl. Acad. Sci. U.S.A. 91:8890-8894(1994) [PubMed: 7522325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"A second subunit of the olfactory cyclic nucleotide-gated channel confers high sensitivity to cAMP." Liman E.R., Buck L.B. Neuron 13:611-621(1994) [PubMed: 7522482] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Olfactory neuroepithelium.
[3]	"Characterization and functional expression of cyclic nucleotide-gated channels in rat pituitary gonadotrophs." Lozach A., Castel H.L.N., Garrel G., Vaudry H., Counis R. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-191. Strain: Sprague-Dawley.
[4]	Bradley J., Zhang Y., Bakin R., Lester H.A., Ronnett G., Zinn K. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 7-35. Strain: Sprague-Dawley.
[5]	"Calmodulin permanently associates with rat olfactory CNG channels under native conditions." Bradley J., Boenigk W., Yau K.-W., Frings S. Nat. Neurosci. 7:705-710(2004) [PubMed: 15195096] [Abstract] Cited for: ENZYME REGULATION.
[6]	"Stoichiometry and assembly of olfactory cyclic nucleotide-gated channels." Zheng J., Zagotta W.N. Neuron 42:411-421(2004) [PubMed: 15134638] [Abstract] Cited for: SUBUNITS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12623 mRNA. Translation: AAA21464.1. U12425 mRNA. Translation: AAA64748.1. AY564233 Genomic DNA. Translation: AAS87325.1. U76219 Genomic DNA. Translation: AAC17596.1.
IPI	IPI00211737.
PIR	I59327.
RefSeq	NP_445948.2. NM_053496.2.
UniGene	Rn.91104.
3D structure databases
ProteinModelPortal	Q64359.
ModBase	Search...
Protein-protein interaction databases
STRING	Q64359.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	85258.
KEGG	rno:85258.
UCSC	U12425. rat.
Organism-specific databases
CTD	1262.
RGD	619844. Cnga4.
Phylogenomic databases
eggNOG	roNOG08807.
GeneTree	ENSGT00550000074376.
HOVERGEN	HBG000281.
InParanoid	Q64359.
OrthoDB	EOG4229JG.
Gene expression databases
ArrayExpress	Q64359.
Genevestigator	Q64359.
GermOnline	ENSRNOG00000017609. Rattus norvegicus.
Family and domain databases
InterPro	IPR018490. cNMP-bd-like. IPR018488. cNMP-bd_CS. IPR000595. cNMP-bd_dom. IPR005821. Ion_trans. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
KO	K04951.
Pfam	PF00027. cNMP_binding. 1 hit. PF00520. Ion_trans. 1 hit. [Graphical view]
SMART	SM00100. cNMP. 1 hit. [Graphical view]
SUPFAM	SSF51206. cNMP_binding. 1 hit.
PROSITE	PS00888. CNMP_BINDING_1. 1 hit. PS00889. CNMP_BINDING_2. 1 hit. PS50042. CNMP_BINDING_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	617376.

Entry information

Entry name

CNGA4_RAT

Accession

Primary (citable) accession number: Q64359
Secondary accession number(s): Q6Q2I4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents