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Protein

Chloride channel protein 1

Gene

Clcn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189ChlorideBy similarity1
Binding sitei578ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

  • chloride transmembrane transport Source: UniProtKB
  • chloride transport Source: MGI
  • muscle contraction Source: UniProtKB
  • neuronal action potential propagation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein 1
Short name:
ClC-1
Alternative name(s):
Chloride channel protein, skeletal muscle
Gene namesi
Name:Clcn1
Synonyms:Clc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88417. Clcn1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 114CytoplasmicBy similarityAdd BLAST114
Transmembranei115 – 152HelicalBy similarityAdd BLAST38
Transmembranei159 – 182HelicalBy similarityAdd BLAST24
Intramembranei191 – 198HelicalBy similarity8
Transmembranei207 – 225HelicalBy similarityAdd BLAST19
Transmembranei232 – 250HelicalBy similarityAdd BLAST19
Intramembranei266 – 278HelicalBy similarityAdd BLAST13
Intramembranei282 – 290HelicalBy similarity9
Transmembranei302 – 321HelicalBy similarityAdd BLAST20
Transmembranei348 – 376HelicalBy similarityAdd BLAST29
Transmembranei385 – 404HelicalBy similarityAdd BLAST20
Transmembranei454 – 474HelicalBy similarityAdd BLAST21
Transmembranei482 – 505HelicalBy similarityAdd BLAST24
Intramembranei522 – 536HelicalBy similarityAdd BLAST15
Intramembranei537 – 538Note=Loop between two helicesBy similarity2
Intramembranei539 – 550HelicalBy similarityAdd BLAST12
Intramembranei551 – 555Note=Loop between two helicesBy similarity5
Transmembranei556 – 573HelicalBy similarityAdd BLAST18
Topological domaini574 – 994CytoplasmicBy similarityAdd BLAST421

GO - Cellular componenti

  • chloride channel complex Source: UniProtKB-KW
  • integral component of plasma membrane Source: UniProtKB
  • sarcolemma Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi553I → T: Causes myotonia. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944301 – 994Chloride channel protein 1Add BLAST994

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei892PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64347.
PRIDEiQ64347.

PTM databases

iPTMnetiQ64347.
PhosphoSitePlusiQ64347.

Expressioni

Tissue specificityi

Predominantly expressed in skeletal muscles.

Gene expression databases

BgeeiENSMUSG00000029862.
CleanExiMM_CLCN1.
ExpressionAtlasiQ64347. baseline and differential.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031894.

Structurei

3D structure databases

ProteinModelPortaliQ64347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini609 – 668CBS 1PROSITE-ProRule annotationAdd BLAST60
Domaini827 – 882CBS 2PROSITE-ProRule annotationAdd BLAST56

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi188 – 192Selectivity filter part_1By similarity5
Motifi230 – 234Selectivity filter part_2By similarity5
Motifi482 – 486Selectivity filter part_3By similarity5

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiQ64347.
KOiK05010.
OMAiPREAINS.
OrthoDBiEOG091G01RJ.
PhylomeDBiQ64347.
TreeFamiTF352264.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002243. Cl_channel-1.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01112. CLCHANNEL1.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64347-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERSQSQRHG GEQSWWGSAP QYQYMPFEHC TSYGLPSENG GLQHRPRKDM
60 70 80 90 100
GPRHNAHPTQ IYGHQKEQYS YKAQDGGMPK KMGSSSTMDS LDEDHYSKCQ
110 120 130 140 150
DCVHRLGRVL RRKLGEDWIF LVLLGLLMAL VSWCMDYVSA KSLQAYKWTY
160 170 180 190 200
AQMKPSLPLQ YLAWVTFPLI LILFSALFCQ LISPQAVGSG IPEMKTILRG
210 220 230 240 250
VVLKEYLTLK AFVAKVVALT AGLGSGIPVG KEGPFVHIAS ICAAVLSKFM
260 270 280 290 300
SMFSGVYEQP YYYTDILTVG CAVGVGCCFG TPLGGVLFSI EVTSTYFAVR
310 320 330 340 350
NYWRGFFAAT FSAFVFRVLA VWNKDAVTIT ALFRTNFRMD FPFDLKELPA
360 370 380 390 400
FAVIGICCGF LGAVFVYLHR QVMLGVRKHK CLSQFLAKHR LLYPGIVTFV
410 420 430 440 450
IASLTFPPGM GQFMAGELMP REAISTLFDN NTWVKHIGDP QSLGQSAVWL
460 470 480 490 500
HPQVNVIIII LLFFVMKFWM SIVATTMPIP CGGFMPVFVL GAAFGRLVGE
510 520 530 540 550
IMAMLFPEGI LFDDIIYKIL PGGYAVIGAA ALTGAVSHTV STAVICFELT
560 570 580 590 600
GQIAHILPMM VAVILANMVA QSLQPSLYDS IIQVKKLPYL PDLGWNQLSK
610 620 630 640 650
FTIFVEDIMV RDVKFVSASC TYGELRNLLQ ATTVKTLPLV DSKDSMILLG
660 670 680 690 700
SVERSELQSL LQRHLCAERR LKAAQDMARK LSELPYNGKA QLAGDWHPGG
710 720 730 740 750
RPESFAFVDE DEDEDLSRKM ELPLTPAPPP PSPPPPPSQF PIAPSNPEEP
760 770 780 790 800
NGPLPSHKQP PEASDSADQR SSTFQRLLHC LLGKAHSKKK KITQDSTDLV
810 820 830 840 850
DNMSPEEIEA WEREQLSQPV CFDCCCIDQS PFQLVEQTTL HKTHTLFSLL
860 870 880 890 900
GLHLAYVTSM GKLRGVLALE ELQKAIEGHT KSGVQLRPPL ASFRNTTSIR
910 920 930 940 950
KTPGGPPPPA EGWNVPEDGD GAPGREVMVP TMPETPVPPP SPEAPSCLAP
960 970 980 990
ARAEGELEEL EMVGSLEPEE ELADILHGPS LRSTDEEDED ELIL
Length:994
Mass (Da):109,794
Last modified:May 18, 2010 - v3
Checksum:i3C523C4325B279BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65Q → H in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti72K → Q in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti76 – 78GGM → RGI in CAB08359 (PubMed:9321463).Curated3
Sequence conflicti82 – 83MG → TD in CAB08359 (PubMed:9321463).Curated2
Sequence conflicti88M → V in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti243A → T in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti483 – 484GF → VN in CAB08359 (PubMed:9321463).Curated2
Sequence conflicti585K → E in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti620C → S in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti631A → T in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti746N → H no nucleotide entry (PubMed:8119941).Curated1
Sequence conflicti864R → T in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti877E → K in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti912G → S in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti918 – 919DG → GE in CAB08359 (PubMed:9321463).Curated2
Sequence conflicti924G → E in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti944A → V no nucleotide entry (PubMed:8119941).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z95127
, Z95128, Z95129, Z95130, Z95131, Z95132, Z95133, Z95134, Z95135, Z95136, Z95137, Z95138, Z95139, Z95140, Z95141, Z95142, Z95143, Z95144, Z95145, Z95146, Z95147, Z95148, Z95149 Genomic DNA. Translation: CAB08359.1.
AK036735 mRNA. Translation: BAC29557.1.
CH466533 Genomic DNA. Translation: EDL13488.1.
BC114336 mRNA. Translation: AAI14337.1.
X62895 mRNA. Translation: CAA44684.1.
X62896 mRNA. Translation: CAA44685.1.
X62897 mRNA. Translation: CAA44686.1.
CCDSiCCDS39471.1.
PIRiI48773.
RefSeqiNP_038519.1. NM_013491.2.
UniGeneiMm.386757.

Genome annotation databases

EnsembliENSMUST00000031894; ENSMUSP00000031894; ENSMUSG00000029862.
GeneIDi12723.
KEGGimmu:12723.
UCSCiuc009bqs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z95127
, Z95128, Z95129, Z95130, Z95131, Z95132, Z95133, Z95134, Z95135, Z95136, Z95137, Z95138, Z95139, Z95140, Z95141, Z95142, Z95143, Z95144, Z95145, Z95146, Z95147, Z95148, Z95149 Genomic DNA. Translation: CAB08359.1.
AK036735 mRNA. Translation: BAC29557.1.
CH466533 Genomic DNA. Translation: EDL13488.1.
BC114336 mRNA. Translation: AAI14337.1.
X62895 mRNA. Translation: CAA44684.1.
X62896 mRNA. Translation: CAA44685.1.
X62897 mRNA. Translation: CAA44686.1.
CCDSiCCDS39471.1.
PIRiI48773.
RefSeqiNP_038519.1. NM_013491.2.
UniGeneiMm.386757.

3D structure databases

ProteinModelPortaliQ64347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031894.

PTM databases

iPTMnetiQ64347.
PhosphoSitePlusiQ64347.

Proteomic databases

PaxDbiQ64347.
PRIDEiQ64347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031894; ENSMUSP00000031894; ENSMUSG00000029862.
GeneIDi12723.
KEGGimmu:12723.
UCSCiuc009bqs.1. mouse.

Organism-specific databases

CTDi1180.
MGIiMGI:88417. Clcn1.

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiQ64347.
KOiK05010.
OMAiPREAINS.
OrthoDBiEOG091G01RJ.
PhylomeDBiQ64347.
TreeFamiTF352264.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiQ64347.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029862.
CleanExiMM_CLCN1.
ExpressionAtlasiQ64347. baseline and differential.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002243. Cl_channel-1.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01112. CLCHANNEL1.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLCN1_MOUSE
AccessioniPrimary (citable) accession number: Q64347
Secondary accession number(s): Q8BZ41, Q9QVY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 18, 2010
Last modified: November 2, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.