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Protein

Chloride channel protein 1

Gene

Clcn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei189 – 1891ChlorideBy similarity
Binding sitei578 – 5781ChlorideBy similarity

GO - Molecular functioni

GO - Biological processi

  • chloride transport Source: MGI
  • neuronal action potential propagation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiREACT_346473. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein 1
Short name:
ClC-1
Alternative name(s):
Chloride channel protein, skeletal muscle
Gene namesi
Name:Clcn1
Synonyms:Clc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88417. Clcn1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 114114CytoplasmicBy similarityAdd
BLAST
Transmembranei115 – 15238HelicalBy similarityAdd
BLAST
Transmembranei159 – 18224HelicalBy similarityAdd
BLAST
Intramembranei191 – 1988HelicalBy similarity
Transmembranei207 – 22519HelicalBy similarityAdd
BLAST
Transmembranei232 – 25019HelicalBy similarityAdd
BLAST
Intramembranei266 – 27813HelicalBy similarityAdd
BLAST
Intramembranei282 – 2909HelicalBy similarity
Transmembranei302 – 32120HelicalBy similarityAdd
BLAST
Transmembranei348 – 37629HelicalBy similarityAdd
BLAST
Transmembranei385 – 40420HelicalBy similarityAdd
BLAST
Transmembranei454 – 47421HelicalBy similarityAdd
BLAST
Transmembranei482 – 50524HelicalBy similarityAdd
BLAST
Intramembranei522 – 53615HelicalBy similarityAdd
BLAST
Intramembranei537 – 5382Note=Loop between two helicesBy similarity
Intramembranei539 – 55012HelicalBy similarityAdd
BLAST
Intramembranei551 – 5555Note=Loop between two helicesBy similarity
Transmembranei556 – 57318HelicalBy similarityAdd
BLAST
Topological domaini574 – 994421CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • chloride channel complex Source: UniProtKB-KW
  • sarcolemma Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi553 – 5531I → T: Causes myotonia. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 994994Chloride channel protein 1PRO_0000094430Add
BLAST

Proteomic databases

PaxDbiQ64347.
PRIDEiQ64347.

PTM databases

PhosphoSiteiQ64347.

Expressioni

Tissue specificityi

Predominantly expressed in skeletal muscles.

Gene expression databases

BgeeiQ64347.
CleanExiMM_CLCN1.
ExpressionAtlasiQ64347. baseline and differential.
GenevisibleiQ64347. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031894.

Structurei

3D structure databases

ProteinModelPortaliQ64347.
SMRiQ64347. Positions 120-670, 803-878.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini609 – 66860CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini827 – 88256CBS 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi188 – 1925Selectivity filter part_1By similarity
Motifi230 – 2345Selectivity filter part_2By similarity
Motifi482 – 4865Selectivity filter part_3By similarity

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0038.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiQ64347.
KOiK05010.
OMAiFTFPPGM.
OrthoDBiEOG77WWCD.
PhylomeDBiQ64347.
TreeFamiTF352264.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002243. Cl_channel-1.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01112. CLCHANNEL1.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64347-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERSQSQRHG GEQSWWGSAP QYQYMPFEHC TSYGLPSENG GLQHRPRKDM
60 70 80 90 100
GPRHNAHPTQ IYGHQKEQYS YKAQDGGMPK KMGSSSTMDS LDEDHYSKCQ
110 120 130 140 150
DCVHRLGRVL RRKLGEDWIF LVLLGLLMAL VSWCMDYVSA KSLQAYKWTY
160 170 180 190 200
AQMKPSLPLQ YLAWVTFPLI LILFSALFCQ LISPQAVGSG IPEMKTILRG
210 220 230 240 250
VVLKEYLTLK AFVAKVVALT AGLGSGIPVG KEGPFVHIAS ICAAVLSKFM
260 270 280 290 300
SMFSGVYEQP YYYTDILTVG CAVGVGCCFG TPLGGVLFSI EVTSTYFAVR
310 320 330 340 350
NYWRGFFAAT FSAFVFRVLA VWNKDAVTIT ALFRTNFRMD FPFDLKELPA
360 370 380 390 400
FAVIGICCGF LGAVFVYLHR QVMLGVRKHK CLSQFLAKHR LLYPGIVTFV
410 420 430 440 450
IASLTFPPGM GQFMAGELMP REAISTLFDN NTWVKHIGDP QSLGQSAVWL
460 470 480 490 500
HPQVNVIIII LLFFVMKFWM SIVATTMPIP CGGFMPVFVL GAAFGRLVGE
510 520 530 540 550
IMAMLFPEGI LFDDIIYKIL PGGYAVIGAA ALTGAVSHTV STAVICFELT
560 570 580 590 600
GQIAHILPMM VAVILANMVA QSLQPSLYDS IIQVKKLPYL PDLGWNQLSK
610 620 630 640 650
FTIFVEDIMV RDVKFVSASC TYGELRNLLQ ATTVKTLPLV DSKDSMILLG
660 670 680 690 700
SVERSELQSL LQRHLCAERR LKAAQDMARK LSELPYNGKA QLAGDWHPGG
710 720 730 740 750
RPESFAFVDE DEDEDLSRKM ELPLTPAPPP PSPPPPPSQF PIAPSNPEEP
760 770 780 790 800
NGPLPSHKQP PEASDSADQR SSTFQRLLHC LLGKAHSKKK KITQDSTDLV
810 820 830 840 850
DNMSPEEIEA WEREQLSQPV CFDCCCIDQS PFQLVEQTTL HKTHTLFSLL
860 870 880 890 900
GLHLAYVTSM GKLRGVLALE ELQKAIEGHT KSGVQLRPPL ASFRNTTSIR
910 920 930 940 950
KTPGGPPPPA EGWNVPEDGD GAPGREVMVP TMPETPVPPP SPEAPSCLAP
960 970 980 990
ARAEGELEEL EMVGSLEPEE ELADILHGPS LRSTDEEDED ELIL
Length:994
Mass (Da):109,794
Last modified:May 18, 2010 - v3
Checksum:i3C523C4325B279BF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651Q → H in CAB08359 (PubMed:9321463).Curated
Sequence conflicti72 – 721K → Q in CAB08359 (PubMed:9321463).Curated
Sequence conflicti76 – 783GGM → RGI in CAB08359 (PubMed:9321463).Curated
Sequence conflicti82 – 832MG → TD in CAB08359 (PubMed:9321463).Curated
Sequence conflicti88 – 881M → V in CAB08359 (PubMed:9321463).Curated
Sequence conflicti243 – 2431A → T in CAB08359 (PubMed:9321463).Curated
Sequence conflicti483 – 4842GF → VN in CAB08359 (PubMed:9321463).Curated
Sequence conflicti585 – 5851K → E in CAB08359 (PubMed:9321463).Curated
Sequence conflicti620 – 6201C → S in CAB08359 (PubMed:9321463).Curated
Sequence conflicti631 – 6311A → T in CAB08359 (PubMed:9321463).Curated
Sequence conflicti746 – 7461N → H no nucleotide entry (PubMed:8119941).Curated
Sequence conflicti864 – 8641R → T in CAB08359 (PubMed:9321463).Curated
Sequence conflicti877 – 8771E → K in CAB08359 (PubMed:9321463).Curated
Sequence conflicti912 – 9121G → S in CAB08359 (PubMed:9321463).Curated
Sequence conflicti918 – 9192DG → GE in CAB08359 (PubMed:9321463).Curated
Sequence conflicti924 – 9241G → E in CAB08359 (PubMed:9321463).Curated
Sequence conflicti944 – 9441A → V no nucleotide entry (PubMed:8119941).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z95127
, Z95128, Z95129, Z95130, Z95131, Z95132, Z95133, Z95134, Z95135, Z95136, Z95137, Z95138, Z95139, Z95140, Z95141, Z95142, Z95143, Z95144, Z95145, Z95146, Z95147, Z95148, Z95149 Genomic DNA. Translation: CAB08359.1.
AK036735 mRNA. Translation: BAC29557.1.
CH466533 Genomic DNA. Translation: EDL13488.1.
BC114336 mRNA. Translation: AAI14337.1.
X62895 mRNA. Translation: CAA44684.1.
X62896 mRNA. Translation: CAA44685.1.
X62897 mRNA. Translation: CAA44686.1.
CCDSiCCDS39471.1.
PIRiI48773.
RefSeqiNP_038519.1. NM_013491.2.
UniGeneiMm.386757.

Genome annotation databases

EnsembliENSMUST00000031894; ENSMUSP00000031894; ENSMUSG00000029862.
GeneIDi12723.
KEGGimmu:12723.
UCSCiuc009bqs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z95127
, Z95128, Z95129, Z95130, Z95131, Z95132, Z95133, Z95134, Z95135, Z95136, Z95137, Z95138, Z95139, Z95140, Z95141, Z95142, Z95143, Z95144, Z95145, Z95146, Z95147, Z95148, Z95149 Genomic DNA. Translation: CAB08359.1.
AK036735 mRNA. Translation: BAC29557.1.
CH466533 Genomic DNA. Translation: EDL13488.1.
BC114336 mRNA. Translation: AAI14337.1.
X62895 mRNA. Translation: CAA44684.1.
X62896 mRNA. Translation: CAA44685.1.
X62897 mRNA. Translation: CAA44686.1.
CCDSiCCDS39471.1.
PIRiI48773.
RefSeqiNP_038519.1. NM_013491.2.
UniGeneiMm.386757.

3D structure databases

ProteinModelPortaliQ64347.
SMRiQ64347. Positions 120-670, 803-878.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031894.

PTM databases

PhosphoSiteiQ64347.

Proteomic databases

PaxDbiQ64347.
PRIDEiQ64347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031894; ENSMUSP00000031894; ENSMUSG00000029862.
GeneIDi12723.
KEGGimmu:12723.
UCSCiuc009bqs.1. mouse.

Organism-specific databases

CTDi1180.
MGIiMGI:88417. Clcn1.

Phylogenomic databases

eggNOGiCOG0038.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiQ64347.
KOiK05010.
OMAiFTFPPGM.
OrthoDBiEOG77WWCD.
PhylomeDBiQ64347.
TreeFamiTF352264.

Enzyme and pathway databases

ReactomeiREACT_346473. Stimuli-sensing channels.

Miscellaneous databases

NextBioi282008.
PROiQ64347.
SOURCEiSearch...

Gene expression databases

BgeeiQ64347.
CleanExiMM_CLCN1.
ExpressionAtlasiQ64347. baseline and differential.
GenevisibleiQ64347. MM.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002243. Cl_channel-1.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01112. CLCHANNEL1.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse Clc1/myotonia gene: ETn insertion, a variable AATC repeat, and PCR diagnosis of alleles."
    Schnuelle V., Antropova O., Gronemeier M., Wedemeyer N., Jockusch H., Bartsch J.W.
    Mamm. Genome 8:718-725(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Inactivation of muscle chloride channel by transposon insertion in myotonic mice."
    Steinmeyer K., Klocke R., Ortland C., Gronemeier M., Jockusch H., Gruender S., Jentsch T.J.
    Nature 354:304-308(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 350-467.
  6. "Nonsense and missense mutations in the muscular chloride channel gene Clc-1 of myotonic mice."
    Gronemeier M., Condie A., Prosser J., Steinmeyer K., Jentsch T.J., Jockusch H.
    J. Biol. Chem. 269:5963-5967(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 543-563; 740-760 AND 941-961, MUTAGENESIS OF ILE-553.
    Strain: C57BL/6 X CBA/CaJ and SWR/J.

Entry informationi

Entry nameiCLCN1_MOUSE
AccessioniPrimary (citable) accession number: Q64347
Secondary accession number(s): Q8BZ41, Q9QVY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 18, 2010
Last modified: June 24, 2015
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.