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Q64346 (DUS6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual specificity protein phosphatase 6

EC=3.1.3.16
EC=3.1.3.48
Alternative name(s):
Mitogen-activated protein kinase phosphatase 3
Short name=MAP kinase phosphatase 3
Short name=MKP-3
Gene names
Name:Dusp6
Synonyms:Mkp3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inactivates MAP kinases. Has a specificity for the ERK family. Implicated in muscle and neuronal differentiation.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Contains 1 rhodanese domain.

Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionHydrolase
Protein phosphatase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from expression pattern Ref.2. Source: RGD

dorsal/ventral pattern formation

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of ERK1 and ERK2 cascade

Inferred from direct assay Ref.1. Source: RGD

positive regulation of apoptotic process

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of cell death

Inferred from mutant phenotype PubMed 21300799. Source: RGD

protein dephosphorylation

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of endodermal cell fate specification

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of fibroblast growth factor receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of heart growth

Inferred from Biological aspect of Ancestor. Source: RefGenome

response to drug

Inferred from expression pattern PubMed 11701771PubMed 16799812PubMed 18958736. Source: RGD

response to growth factor

Inferred from expression pattern PubMed 9559664. Source: RGD

response to nitrosative stress

Inferred from electronic annotation. Source: Ensembl

response to organic cyclic compound

Inferred from expression pattern PubMed 17881770. Source: RGD

response to organic substance

Inferred from expression pattern PubMed 11027531. Source: RGD

   Cellular_componentcytoplasm

Inferred from direct assay Ref.2. Source: RGD

cytosol

Inferred from direct assay Ref.1. Source: RGD

   Molecular_functionMAP kinase tyrosine/serine/threonine phosphatase activity

Inferred from electronic annotation. Source: Ensembl

protein tyrosine phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

protein tyrosine/serine/threonine phosphatase activity

Inferred from direct assay Ref.2. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 381381Dual specificity protein phosphatase 6
PRO_0000094806

Regions

Domain30 – 148119Rhodanese
Domain206 – 381176Tyrosine-protein phosphatase

Sites

Active site2931Phosphocysteine intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64346 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C511E00CB68F2888

FASTA38142,319
        10         20         30         40         50         60 
MIDTLRPVPF ASEMAISKTV AWLNEQLELG NEQLLLMDCR PQELYESSHI ESAINVAIPG 

        70         80         90        100        110        120 
IMLRRLQKGN LPVRALFTRC EDRDRFTRRC GTDTVVLYDE NSSDWNENTG GESVLGLLLK 

       130        140        150        160        170        180 
KLKDEGCRAF YLEGGFSKFQ AEFALHCETN LDGSCSSSSP PLPVLGLGGL RISSDSSSDI 

       190        200        210        220        230        240 
ESDLDRDPNS ATDSDGSPLS NSQPSFPVEI LPFLYLGCAK DSTNLDVLEE FGIKYILNVT 

       250        260        270        280        290        300 
PNLPNLFENA GEFKYKQIPI SDHWSQNLSQ FFPEAISFID EARGKNCGVL VHCLAGISRS 

       310        320        330        340        350        360 
VTVTVAYLMQ KLNLSMNDAY DIVKMKKSNI SPNFNFMGQL LDFERTLGLS SPCDNRVPAQ 

       370        380 
QLYFTAPSNQ NVYQVDSLQS T 

« Hide

References

« Hide 'large scale' references
[1]"MKP-3, a novel cytosolic protein-tyrosine phosphatase that exemplifies a new class of mitogen-activated protein kinase phosphatase."
Muda M., Boschert U., Dickinson R., Martinou J.-C., Martinou I., Camps M., Schlegel W., Arkinstall S.
J. Biol. Chem. 271:4319-4326(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Neuron.
[2]"A novel cytoplasmic dual specificity protein tyrosine phosphatase implicated in muscle and neuronal differentiation."
Mourey R.J., Vega Q.C., Campbell J.S., Wenderoth M.P., Hauschka S.D., Krebs E.G., Dixon J.E.
J. Biol. Chem. 271:3795-3802(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X94185 mRNA. Translation: CAA63895.1.
U42627 mRNA. Translation: AAB06202.1.
BC087003 mRNA. Translation: AAH87003.1.
RefSeqNP_446335.1. NM_053883.2.
UniGeneRn.4313.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FYSX-ray2.50C/D60-76[»]
ProteinModelPortalQ64346.
SMRQ64346. Positions 1-154, 205-347.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000032969.

Chemistry

BindingDBQ64346.
ChEMBLCHEMBL5511.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000037844; ENSRNOP00000032969; ENSRNOG00000023896.
GeneID116663.
KEGGrno:116663.
UCSCRGD:70978. rat.

Organism-specific databases

CTD1848.
RGD70978. Dusp6.

Phylogenomic databases

eggNOGCOG2453.
GeneTreeENSGT00750000117282.
HOGENOMHOG000294079.
HOVERGENHBG007347.
KOK04459.
OMASEWNENT.
OrthoDBEOG793B7W.
PhylomeDBQ64346.
TreeFamTF105122.

Gene expression databases

GenevestigatorQ64346.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR000387. Tyr/Dual-sp_Pase.
[Graphical view]
PANTHERPTHR10159. PTHR10159. 1 hit.
PfamPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PIRSFPIRSF000939. MAPK_Ptase. 1 hit.
PRINTSPR01764. MAPKPHPHTASE.
SMARTSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ64346.
NextBio619455.
PROQ64346.

Entry information

Entry nameDUS6_RAT
AccessionPrimary (citable) accession number: Q64346
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references