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Protein

Dual specificity protein phosphatase 6

Gene

Dusp6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inactivates MAP kinases. Has a specificity for the ERK family (PubMed:8626780). Implicated in muscle and neuronal differentiation (PubMed:8631996). Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (By similarity).By similarity2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei293 – 2931Phosphocysteine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: RGD
  • dorsal/ventral pattern formation Source: GO_Central
  • negative regulation of ERK1 and ERK2 cascade Source: RGD
  • positive regulation of apoptotic process Source: GO_Central
  • positive regulation of cell death Source: RGD
  • regulation of endodermal cell fate specification Source: GO_Central
  • regulation of fibroblast growth factor receptor signaling pathway Source: GO_Central
  • regulation of heart growth Source: GO_Central
  • response to drug Source: RGD
  • response to growth factor Source: RGD
  • response to nitrosative stress Source: Ensembl
  • response to organic cyclic compound Source: RGD
  • response to organic substance Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiR-RNO-112409. RAF-independent MAPK1/3 activation.
R-RNO-202670. ERKs are inactivated.
R-RNO-5675221. Negative regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 6 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Mitogen-activated protein kinase phosphatase 3
Short name:
MAP kinase phosphatase 3
Short name:
MKP-3
Gene namesi
Name:Dusp6
Synonyms:Mkp3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi70978. Dusp6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5511.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 381381Dual specificity protein phosphatase 6PRO_0000094806Add
BLAST

Proteomic databases

PaxDbiQ64346.

PTM databases

iPTMnetiQ64346.

Expressioni

Tissue specificityi

Expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis.1 Publication

Gene expression databases

GenevisibleiQ64346. RN.

Interactioni

Protein-protein interaction databases

DIPiDIP-61143N.
STRINGi10116.ENSRNOP00000032969.

Chemistry

BindingDBiQ64346.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FYSX-ray2.50C/D60-76[»]
ProteinModelPortaliQ64346.
SMRiQ64346. Positions 1-154, 205-347.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ64346.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 148119RhodanesePROSITE-ProRule annotationAdd
BLAST
Domaini206 – 381176Tyrosine-protein phosphataseAdd
BLAST

Sequence similaritiesi

Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1717. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118902.
HOGENOMiHOG000294079.
HOVERGENiHBG007347.
InParanoidiQ64346.
KOiK04459.
OMAiRDWNENT.
OrthoDBiEOG793B7W.
PhylomeDBiQ64346.
TreeFamiTF105122.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PIRSFiPIRSF000939. MAPK_Ptase. 1 hit.
PRINTSiPR01764. MAPKPHPHTASE.
SMARTiSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64346-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDTLRPVPF ASEMAISKTV AWLNEQLELG NEQLLLMDCR PQELYESSHI
60 70 80 90 100
ESAINVAIPG IMLRRLQKGN LPVRALFTRC EDRDRFTRRC GTDTVVLYDE
110 120 130 140 150
NSSDWNENTG GESVLGLLLK KLKDEGCRAF YLEGGFSKFQ AEFALHCETN
160 170 180 190 200
LDGSCSSSSP PLPVLGLGGL RISSDSSSDI ESDLDRDPNS ATDSDGSPLS
210 220 230 240 250
NSQPSFPVEI LPFLYLGCAK DSTNLDVLEE FGIKYILNVT PNLPNLFENA
260 270 280 290 300
GEFKYKQIPI SDHWSQNLSQ FFPEAISFID EARGKNCGVL VHCLAGISRS
310 320 330 340 350
VTVTVAYLMQ KLNLSMNDAY DIVKMKKSNI SPNFNFMGQL LDFERTLGLS
360 370 380
SPCDNRVPAQ QLYFTAPSNQ NVYQVDSLQS T
Length:381
Mass (Da):42,319
Last modified:November 1, 1996 - v1
Checksum:iC511E00CB68F2888
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94185 mRNA. Translation: CAA63895.1.
U42627 mRNA. Translation: AAB06202.1.
BC087003 mRNA. Translation: AAH87003.1.
RefSeqiNP_446335.1. NM_053883.2.
UniGeneiRn.4313.

Genome annotation databases

EnsembliENSRNOT00000037844; ENSRNOP00000032969; ENSRNOG00000023896.
GeneIDi116663.
KEGGirno:116663.
UCSCiRGD:70978. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94185 mRNA. Translation: CAA63895.1.
U42627 mRNA. Translation: AAB06202.1.
BC087003 mRNA. Translation: AAH87003.1.
RefSeqiNP_446335.1. NM_053883.2.
UniGeneiRn.4313.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FYSX-ray2.50C/D60-76[»]
ProteinModelPortaliQ64346.
SMRiQ64346. Positions 1-154, 205-347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61143N.
STRINGi10116.ENSRNOP00000032969.

Chemistry

BindingDBiQ64346.
ChEMBLiCHEMBL5511.

PTM databases

iPTMnetiQ64346.

Proteomic databases

PaxDbiQ64346.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000037844; ENSRNOP00000032969; ENSRNOG00000023896.
GeneIDi116663.
KEGGirno:116663.
UCSCiRGD:70978. rat.

Organism-specific databases

CTDi1848.
RGDi70978. Dusp6.

Phylogenomic databases

eggNOGiKOG1717. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118902.
HOGENOMiHOG000294079.
HOVERGENiHBG007347.
InParanoidiQ64346.
KOiK04459.
OMAiRDWNENT.
OrthoDBiEOG793B7W.
PhylomeDBiQ64346.
TreeFamiTF105122.

Enzyme and pathway databases

ReactomeiR-RNO-112409. RAF-independent MAPK1/3 activation.
R-RNO-202670. ERKs are inactivated.
R-RNO-5675221. Negative regulation of MAPK pathway.

Miscellaneous databases

EvolutionaryTraceiQ64346.
PROiQ64346.

Gene expression databases

GenevisibleiQ64346. RN.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PIRSFiPIRSF000939. MAPK_Ptase. 1 hit.
PRINTSiPR01764. MAPKPHPHTASE.
SMARTiSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MKP-3, a novel cytosolic protein-tyrosine phosphatase that exemplifies a new class of mitogen-activated protein kinase phosphatase."
    Muda M., Boschert U., Dickinson R., Martinou J.-C., Martinou I., Camps M., Schlegel W., Arkinstall S.
    J. Biol. Chem. 271:4319-4326(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Neuron.
  2. "A novel cytoplasmic dual specificity protein tyrosine phosphatase implicated in muscle and neuronal differentiation."
    Mourey R.J., Vega Q.C., Campbell J.S., Wenderoth M.P., Hauschka S.D., Krebs E.G., Dixon J.E.
    J. Biol. Chem. 271:3795-3802(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiDUS6_RAT
AccessioniPrimary (citable) accession number: Q64346
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.