ATP-binding cassette sub-family G member 1

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Q64343 (ABCG1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 118. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
ATP-binding cassette sub-family G member 1

Alternative name(s):
ATP-binding cassette transporter 8
White protein homolog

Gene names

Name:	Abcg1
Synonyms:	Abc8, Wht1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	666 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Ref.4 Ref.6
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed mainly in brain, thymus, lung, adrenals, spleen and placenta. Little or no expression in liver, kidney, heart, muscle or testes.
Induction	Strongly induced in macrophage cell line RAW 264.7 during cholesterol influx. Induction is mediated by the liver X receptor/retinoid X receptor (LXR/RXR) pathway. Down-regulated by endotoxins or cytokines (TNF and IL1) in J-774 macrophages. Ref.4 Ref.7
Post-translational modification	Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum By similarity. Ref.8
Sequence similarities	Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. [View classification] Contains 1 ABC transmembrane type-2 domain. Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC amyloid precursor protein catabolic process Inferred from electronic annotation. Source: Compara cholesterol efflux Inferred from mutant phenotype PubMed 16556852. Source: BHF-UCL cholesterol homeostasis Inferred from electronic annotation. Source: Compara cholesterol metabolic process Inferred from electronic annotation. Source: Compara glycoprotein transport Inferred from electronic annotation. Source: Compara high-density lipoprotein particle remodeling Inferred from mutant phenotype PubMed 16556852. Source: BHF-UCL intracellular cholesterol transport Inferred from electronic annotation. Source: Compara low-density lipoprotein particle remodeling Inferred from mutant phenotype PubMed 16556852. Source: BHF-UCL negative regulation of lipid storage Inferred from mutant phenotype PubMed 16556852. Source: BHF-UCL negative regulation of macrophage derived foam cell differentiation Inferred by curator PubMed 16556852. Source: BHF-UCL phospholipid efflux Inferred from electronic annotation. Source: Compara phospholipid homeostasis Inferred from electronic annotation. Source: Compara positive regulation of cholesterol biosynthetic process Inferred from mutant phenotype PubMed 17916878. Source: BHF-UCL positive regulation of cholesterol efflux Inferred from direct assay PubMed 15210959. Source: BHF-UCL regulation of cholesterol esterification Inferred from mutant phenotype PubMed 16556852. Source: BHF-UCL regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 17916878. Source: BHF-UCL response to high density lipoprotein particle stimulus Inferred from direct assay PubMed 15210959. Source: BHF-UCL response to lipid Inferred from electronic annotation. Source: Compara reverse cholesterol transport Inferred from mutant phenotype PubMed 17657311. Source: BHF-UCL toxin transport Inferred from electronic annotation. Source: GOC
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara external side of plasma membrane Inferred from direct assay PubMed 16556852. Source: BHF-UCL integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from electronic annotation. Source: Compara recycling endosome Inferred from direct assay PubMed 16556852. Source: BHF-UCL
Molecular_function	ADP binding Inferred from electronic annotation. Source: Compara ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cholesterol transporter activity Inferred from electronic annotation. Source: Compara glycoprotein transporter activity Inferred from electronic annotation. Source: Compara phospholipid transporter activity Inferred from electronic annotation. Source: Compara sterol-transporting ATPase activity Inferred from electronic annotation. Source: Compara toxin transporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 666

666

ATP-binding cassette sub-family G member 1

PRO_0000093385

Regions

Topological domain

1 – 414

414

Cytoplasmic Potential

Transmembrane

415 – 433

Helical; Potential

Topological domain

434 – 444

Extracellular Potential

Transmembrane

445 – 465

Helical; Potential

Topological domain

466 – 494

Cytoplasmic Potential

Transmembrane

495 – 513

Helical; Potential

Topological domain

514 – 521

Extracellular Potential

Transmembrane

522 – 543

Helical; Potential

Topological domain

544 – 555

Cytoplasmic Potential

Transmembrane

556 – 574

Helical; Potential

Topological domain

575 – 637

Extracellular Potential

Transmembrane

638 – 657

Helical; Potential

Topological domain

658 – 666

Cytoplasmic Potential

Domain

77 – 317

241

ABC transporter

Domain

403 – 661

259

ABC transmembrane type-2

Nucleotide binding

118 – 125

ATP Potential

Amino acid modifications

Lipidation

S-palmitoyl cysteine By similarity

Lipidation

154

S-palmitoyl cysteine By similarity

Lipidation

315

S-palmitoyl cysteine By similarity

Lipidation

394

S-palmitoyl cysteine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q64343 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: EDDC6AFBD43950B6
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FASTA

666

74,033

        10         20         30         40         50         60 
MACLMAAFSV GTAMNASSYS AAMTEPKSVC VSVDEVVSSN VDEVETDLLN GHLKKVDNNF 

        70         80         90        100        110        120 
TEAQRFSSLP RRAAVNIEFK DLSYSVPEGP WWKKKGYKTL LKGISGKFNS GELVAIMGPS 

       130        140        150        160        170        180 
GAGKSTLMNI LAGYRETGMK GAVLINGMPR DLRCFRKVSC YIMQDDMLLP HLTVQEAMMV 

       190        200        210        220        230        240 
SAHLKLQEKD EGRREMVKEI LTALGLLPCA NTRTGSLSGG QRKRLAIALE LVNNPPVMFF 

       250        260        270        280        290        300 
DEPTSGLDSA SCFQVVSLMK GLAQGGRSIV CTIHQPSAKL FELFDQLYVL SQGQCVYRGK 

       310        320        330        340        350        360 
VSNLVPYLRD LGLNCPTYHN PADFVMEVAS GEYGDQNSRL VRAVREGMCD ADYKRDLGGD 

       370        380        390        400        410        420 
TDVNPFLWHR PAEEDSASME GCHSFSASCL TQFCILFKRT FLSIMRDSVL THLRITSHIG 

       430        440        450        460        470        480 
IGLLIGLLYL GIGNEAKKVL SNSGFLFFSM LFLMFAALMP TVLTFPLEMS VFLREHLNYW 

       490        500        510        520        530        540 
YSLKAYYLAK TMADVPFQIM FPVAYCSIVY WMTSQPSDAV RFVLFAALGT MTSLVAQSLG 

       550        560        570        580        590        600 
LLIGAASTSL QVATFVGPVT AIPVLLFSGF FVSFDTIPAY LQWMSYISYV RYGFEGVILS 

       610        620        630        640        650        660 
IYGLDREDLH CDIAETCHFQ KSEAILRELD VENAKLYLDF IVLGIFFISL RLIAYFVLRY 


KIRAER

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References

[1]	"Isolation and characterization of a mammalian homolog of the Drosophila white gene." Croop J.M., Tiller G.E., Fletcher J.A., Lux M.L., Raab E., Goldenson D., Son D., Arciniegas S., Wu R. Gene 185:77-85(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of a mammalian ABC transporter homologous to Drosophila white gene." Savary S., Denizot F., Luciani M.-F., Mattei M.-G., Chimini G. Mamm. Genome 7:673-676(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: DBA/2.
[3]	"Genomic sequence and structure of the human ABCG1 (ABC8) gene." Lorkowski S., Rust S., Engel T., Jung E., Tegelkamp K., Galinski E.A., Assmann G., Cullen P. Biochem. Biophys. Res. Commun. 280:121-131(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Human white/murine ABC8 mRNA levels are highly induced in lipid-loaded macrophages. A transcriptional role for specific oxysterols." Venkateswaran A., Repa J.J., Lobaccaro J.-M.A., Bronson A., Mangelsdorf D.J., Edwards P.A. J. Biol. Chem. 275:14700-14707(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, PROBABLE FUNCTION.
[5]	"Role of ABCG1 and other ABCG family members in lipid metabolism." Schmitz G., Langmann T., Heimerl S. J. Lipid Res. 42:1513-1520(2001) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[6]	"Physiological function of ABCG1." Ito T. Drug News Perspect. 16:490-492(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: differential role of LXR." Khovidhunkit W., Moser A.H., Shigenaga J.K., Grunfeld C., Feingold K.R. J. Lipid Res. 44:1728-1736(2003) [PubMed] [Europe PMC] [Abstract] Cited for: DOWN-REGULATION BY ENDOTOXIN.
[8]	"Characterization of palmitoylation of ATP binding cassette transporter G1: Effect on protein trafficking and function." Gu H.M., Li G., Gao X., Berthiaume L.G., Zhang D.W. Biochim. Biophys. Acta 0:0-0(2013) [PubMed] [Europe PMC] [Abstract] Cited for: PALMITOYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34920 mRNA. Translation: AAB47738.1. Z48745 mRNA. Translation: CAA88636.1. AF323659 mRNA. Translation: AAK27442.1.
IPI	IPI00133416.
RefSeq	NP_033723.1. NM_009593.2.
UniGene	Mm.15691. Mm.470747.
3D structure databases
ProteinModelPortal	Q64343.
SMR	Q64343. Positions 2-323.
ModBase	Search...
PTM databases
PhosphoSite	Q64343.
Proteomic databases
PaxDb	Q64343.
PRIDE	Q64343.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000024829; ENSMUSP00000024829; ENSMUSG00000024030.
GeneID	11307.
KEGG	mmu:11307.
UCSC	uc008buj.2. mouse.
Organism-specific databases
CTD	9619.
MGI	MGI:107704. Abcg1.
Phylogenomic databases
eggNOG	COG1131.
GeneTree	ENSGT00700000104032.
HOGENOM	HOG000236364.
HOVERGEN	HBG103052.
InParanoid	Q64343.
KO	K05679.
OMA	STEGCHS.
OrthoDB	EOG4WDDBB.
Gene expression databases
ArrayExpress	Q64343.
Bgee	Q64343.
Genevestigator	Q64343.
GermOnline	ENSMUSG00000024030. Mus musculus.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR013525. ABC_2_trans. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR020064. ABC_transptr_G1-like. IPR027417. P-loop_NTPase. IPR005284. Pigment_permease. [Graphical view]
PANTHER	PTHR19241:SF21. PTHR19241:SF21. 1 hit.
Pfam	PF01061. ABC2_membrane. 1 hit. PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00955. 3a01204. 1 hit.
PROSITE	PS51012. ABC_TM2. False negative. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	278604.
SOURCE	Search...

Entry information

Entry name

ABCG1_MOUSE

Accession

Primary (citable) accession number: Q64343

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 29, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents