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Protein

ATP-binding cassette sub-family G member 1

Gene

Abcg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi118 – 1258ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ADP binding Source: MGI
  2. ATP binding Source: MGI
  3. cholesterol transporter activity Source: MGI
  4. glycoprotein transporter activity Source: MGI
  5. phospholipid transporter activity Source: MGI
  6. protein heterodimerization activity Source: MGI
  7. protein homodimerization activity Source: MGI
  8. sterol-transporting ATPase activity Source: MGI
  9. toxin transporter activity Source: MGI

GO - Biological processi

  1. amyloid precursor protein catabolic process Source: MGI
  2. cholesterol efflux Source: BHF-UCL
  3. cholesterol homeostasis Source: MGI
  4. cholesterol metabolic process Source: MGI
  5. cholesterol transport Source: MGI
  6. glycoprotein transport Source: MGI
  7. high-density lipoprotein particle remodeling Source: BHF-UCL
  8. intracellular cholesterol transport Source: MGI
  9. low-density lipoprotein particle remodeling Source: BHF-UCL
  10. negative regulation of lipid storage Source: BHF-UCL
  11. negative regulation of macrophage derived foam cell differentiation Source: BHF-UCL
  12. phospholipid efflux Source: MGI
  13. phospholipid homeostasis Source: MGI
  14. positive regulation of cholesterol biosynthetic process Source: BHF-UCL
  15. positive regulation of cholesterol efflux Source: BHF-UCL
  16. regulation of cholesterol esterification Source: BHF-UCL
  17. regulation of transcription, DNA-templated Source: BHF-UCL
  18. response to high density lipoprotein particle Source: BHF-UCL
  19. response to lipid Source: MGI
  20. response to organic substance Source: MGI
  21. reverse cholesterol transport Source: BHF-UCL
  22. toxin transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_197984. ABCA transporters in lipid homeostasis.
REACT_213857. HDL-mediated lipid transport.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family G member 1
Alternative name(s):
ATP-binding cassette transporter 8
White protein homolog
Gene namesi
Name:Abcg1
Synonyms:Abc8, Wht1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:107704. Abcg1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 414414CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei415 – 43319HelicalSequence AnalysisAdd
BLAST
Topological domaini434 – 44411ExtracellularSequence AnalysisAdd
BLAST
Transmembranei445 – 46521HelicalSequence AnalysisAdd
BLAST
Topological domaini466 – 49429CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei495 – 51319HelicalSequence AnalysisAdd
BLAST
Topological domaini514 – 5218ExtracellularSequence Analysis
Transmembranei522 – 54322HelicalSequence AnalysisAdd
BLAST
Topological domaini544 – 55512CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei556 – 57419HelicalSequence AnalysisAdd
BLAST
Topological domaini575 – 63763ExtracellularSequence AnalysisAdd
BLAST
Transmembranei638 – 65720HelicalSequence AnalysisAdd
BLAST
Topological domaini658 – 6669CytoplasmicSequence Analysis

GO - Cellular componenti

  1. endosome Source: BHF-UCL
  2. external side of plasma membrane Source: BHF-UCL
  3. Golgi apparatus Source: MGI
  4. integral component of membrane Source: UniProtKB-KW
  5. intracellular membrane-bounded organelle Source: MGI
  6. mitochondrion Source: MGI
  7. nucleoplasm Source: MGI
  8. plasma membrane Source: MGI
  9. recycling endosome Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 666666ATP-binding cassette sub-family G member 1PRO_0000093385Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi30 – 301S-palmitoyl cysteineBy similarity
Lipidationi154 – 1541S-palmitoyl cysteineBy similarity
Lipidationi315 – 3151S-palmitoyl cysteineBy similarity
Lipidationi394 – 3941S-palmitoyl cysteineBy similarity

Post-translational modificationi

Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum.By similarity

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

MaxQBiQ64343.
PaxDbiQ64343.
PRIDEiQ64343.

PTM databases

PhosphoSiteiQ64343.

Expressioni

Tissue specificityi

Expressed mainly in brain, thymus, lung, adrenals, spleen and placenta. Little or no expression in liver, kidney, heart, muscle or testes.

Inductioni

Strongly induced in macrophage cell line RAW 264.7 during cholesterol influx. Induction is mediated by the liver X receptor/retinoid X receptor (LXR/RXR) pathway. Down-regulated by endotoxins or cytokines (TNF and IL1) in J-774 macrophages.1 Publication

Gene expression databases

BgeeiQ64343.
ExpressionAtlasiQ64343. baseline and differential.
GenevestigatoriQ64343.

Interactioni

Protein-protein interaction databases

BioGridi197904. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ64343.
SMRiQ64343. Positions 2-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini77 – 317241ABC transporterPROSITE-ProRule annotationAdd
BLAST
Domaini403 – 661259ABC transmembrane type-2Add
BLAST

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1131.
GeneTreeiENSGT00740000114855.
HOGENOMiHOG000236364.
HOVERGENiHBG103052.
InParanoidiQ64343.
KOiK05679.
OMAiREGMCDT.
OrthoDBiEOG7KWSGT.
PhylomeDBiQ64343.
TreeFamiTF105210.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR020064. ABCG1.
IPR027417. P-loop_NTPase.
IPR005284. Pigment_permease.
[Graphical view]
PANTHERiPTHR19241:SF177. PTHR19241:SF177. 1 hit.
PfamiPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00955. 3a01204. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACLMAAFSV GTAMNASSYS AAMTEPKSVC VSVDEVVSSN VDEVETDLLN
60 70 80 90 100
GHLKKVDNNF TEAQRFSSLP RRAAVNIEFK DLSYSVPEGP WWKKKGYKTL
110 120 130 140 150
LKGISGKFNS GELVAIMGPS GAGKSTLMNI LAGYRETGMK GAVLINGMPR
160 170 180 190 200
DLRCFRKVSC YIMQDDMLLP HLTVQEAMMV SAHLKLQEKD EGRREMVKEI
210 220 230 240 250
LTALGLLPCA NTRTGSLSGG QRKRLAIALE LVNNPPVMFF DEPTSGLDSA
260 270 280 290 300
SCFQVVSLMK GLAQGGRSIV CTIHQPSAKL FELFDQLYVL SQGQCVYRGK
310 320 330 340 350
VSNLVPYLRD LGLNCPTYHN PADFVMEVAS GEYGDQNSRL VRAVREGMCD
360 370 380 390 400
ADYKRDLGGD TDVNPFLWHR PAEEDSASME GCHSFSASCL TQFCILFKRT
410 420 430 440 450
FLSIMRDSVL THLRITSHIG IGLLIGLLYL GIGNEAKKVL SNSGFLFFSM
460 470 480 490 500
LFLMFAALMP TVLTFPLEMS VFLREHLNYW YSLKAYYLAK TMADVPFQIM
510 520 530 540 550
FPVAYCSIVY WMTSQPSDAV RFVLFAALGT MTSLVAQSLG LLIGAASTSL
560 570 580 590 600
QVATFVGPVT AIPVLLFSGF FVSFDTIPAY LQWMSYISYV RYGFEGVILS
610 620 630 640 650
IYGLDREDLH CDIAETCHFQ KSEAILRELD VENAKLYLDF IVLGIFFISL
660
RLIAYFVLRY KIRAER
Length:666
Mass (Da):74,033
Last modified:November 1, 1997 - v1
Checksum:iEDDC6AFBD43950B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34920 mRNA. Translation: AAB47738.1.
Z48745 mRNA. Translation: CAA88636.1.
AF323659 mRNA. Translation: AAK27442.1.
CCDSiCCDS28602.1.
RefSeqiNP_033723.1. NM_009593.2.
UniGeneiMm.15691.
Mm.470747.

Genome annotation databases

EnsembliENSMUST00000024829; ENSMUSP00000024829; ENSMUSG00000024030.
GeneIDi11307.
KEGGimmu:11307.
UCSCiuc008buj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34920 mRNA. Translation: AAB47738.1.
Z48745 mRNA. Translation: CAA88636.1.
AF323659 mRNA. Translation: AAK27442.1.
CCDSiCCDS28602.1.
RefSeqiNP_033723.1. NM_009593.2.
UniGeneiMm.15691.
Mm.470747.

3D structure databases

ProteinModelPortaliQ64343.
SMRiQ64343. Positions 2-323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197904. 1 interaction.

PTM databases

PhosphoSiteiQ64343.

Proteomic databases

MaxQBiQ64343.
PaxDbiQ64343.
PRIDEiQ64343.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024829; ENSMUSP00000024829; ENSMUSG00000024030.
GeneIDi11307.
KEGGimmu:11307.
UCSCiuc008buj.2. mouse.

Organism-specific databases

CTDi9619.
MGIiMGI:107704. Abcg1.

Phylogenomic databases

eggNOGiCOG1131.
GeneTreeiENSGT00740000114855.
HOGENOMiHOG000236364.
HOVERGENiHBG103052.
InParanoidiQ64343.
KOiK05679.
OMAiREGMCDT.
OrthoDBiEOG7KWSGT.
PhylomeDBiQ64343.
TreeFamiTF105210.

Enzyme and pathway databases

ReactomeiREACT_197984. ABCA transporters in lipid homeostasis.
REACT_213857. HDL-mediated lipid transport.

Miscellaneous databases

NextBioi278604.
PROiQ64343.
SOURCEiSearch...

Gene expression databases

BgeeiQ64343.
ExpressionAtlasiQ64343. baseline and differential.
GenevestigatoriQ64343.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR020064. ABCG1.
IPR027417. P-loop_NTPase.
IPR005284. Pigment_permease.
[Graphical view]
PANTHERiPTHR19241:SF177. PTHR19241:SF177. 1 hit.
PfamiPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00955. 3a01204. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of a mammalian homolog of the Drosophila white gene."
    Croop J.M., Tiller G.E., Fletcher J.A., Lux M.L., Raab E., Goldenson D., Son D., Arciniegas S., Wu R.
    Gene 185:77-85(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning of a mammalian ABC transporter homologous to Drosophila white gene."
    Savary S., Denizot F., Luciani M.-F., Mattei M.-G., Chimini G.
    Mamm. Genome 7:673-676(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: DBA/2.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Human white/murine ABC8 mRNA levels are highly induced in lipid-loaded macrophages. A transcriptional role for specific oxysterols."
    Venkateswaran A., Repa J.J., Lobaccaro J.-M.A., Bronson A., Mangelsdorf D.J., Edwards P.A.
    J. Biol. Chem. 275:14700-14707(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, PROBABLE FUNCTION.
  5. "Role of ABCG1 and other ABCG family members in lipid metabolism."
    Schmitz G., Langmann T., Heimerl S.
    J. Lipid Res. 42:1513-1520(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  6. "Physiological function of ABCG1."
    Ito T.
    Drug News Perspect. 16:490-492(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: differential role of LXR."
    Khovidhunkit W., Moser A.H., Shigenaga J.K., Grunfeld C., Feingold K.R.
    J. Lipid Res. 44:1728-1736(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOWN-REGULATION BY ENDOTOXIN.
  8. "Characterization of palmitoylation of ATP binding cassette transporter G1: Effect on protein trafficking and function."
    Gu H.M., Li G., Gao X., Berthiaume L.G., Zhang D.W.
    Biochim. Biophys. Acta 1831:1067-1078(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION.

Entry informationi

Entry nameiABCG1_MOUSE
AccessioniPrimary (citable) accession number: Q64343
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 4, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.