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Q64343 (ABCG1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-binding cassette sub-family G member 1
Alternative name(s):
ATP-binding cassette transporter 8
White protein homolog
Gene names
Name:Abcg1
Synonyms:Abc8, Wht1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length666 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Ref.4 Ref.6

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Tissue specificity

Expressed mainly in brain, thymus, lung, adrenals, spleen and placenta. Little or no expression in liver, kidney, heart, muscle or testes.

Induction

Strongly induced in macrophage cell line RAW 264.7 during cholesterol influx. Induction is mediated by the liver X receptor/retinoid X receptor (LXR/RXR) pathway. Down-regulated by endotoxins or cytokines (TNF and IL1) in J-774 macrophages. Ref.4 Ref.7

Post-translational modification

Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum By similarity. Ref.8

Sequence similarities

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. [View classification]

Contains 1 ABC transmembrane type-2 domain.

Contains 1 ABC transporter domain.

Ontologies

Keywords
   Biological processLipid transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   PTMLipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processamyloid precursor protein catabolic process

Inferred from electronic annotation. Source: Ensembl

cholesterol efflux

Inferred from mutant phenotype PubMed 16556852. Source: BHF-UCL

cholesterol homeostasis

Inferred from electronic annotation. Source: Ensembl

cholesterol metabolic process

Inferred from electronic annotation. Source: Ensembl

cholesterol transport

Inferred from direct assay PubMed 15319426. Source: MGI

high-density lipoprotein particle remodeling

Inferred from mutant phenotype PubMed 16556852. Source: BHF-UCL

intracellular cholesterol transport

Inferred from electronic annotation. Source: Ensembl

low-density lipoprotein particle remodeling

Inferred from mutant phenotype PubMed 16556852. Source: BHF-UCL

negative regulation of lipid storage

Inferred from mutant phenotype PubMed 16556852. Source: BHF-UCL

negative regulation of macrophage derived foam cell differentiation

Inferred by curator PubMed 16556852. Source: BHF-UCL

phospholipid efflux

Inferred from electronic annotation. Source: Ensembl

phospholipid homeostasis

Inferred from electronic annotation. Source: Ensembl

positive regulation of cholesterol biosynthetic process

Inferred from mutant phenotype PubMed 17916878. Source: BHF-UCL

positive regulation of cholesterol efflux

Inferred from direct assay PubMed 15210959. Source: BHF-UCL

regulation of cholesterol esterification

Inferred from mutant phenotype PubMed 16556852. Source: BHF-UCL

regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 17916878. Source: BHF-UCL

response to high density lipoprotein particle

Inferred from direct assay PubMed 15210959. Source: BHF-UCL

response to lipid

Inferred from electronic annotation. Source: Ensembl

reverse cholesterol transport

Inferred from mutant phenotype PubMed 17657311. Source: BHF-UCL

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Ensembl

endosome

Inferred from direct assay PubMed 17916878. Source: BHF-UCL

external side of plasma membrane

Inferred from direct assay PubMed 16556852. Source: BHF-UCL

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

mitochondrion

Inferred from electronic annotation. Source: Ensembl

recycling endosome

Inferred from direct assay PubMed 16556852. Source: BHF-UCL

   Molecular_functionADP binding

Inferred from electronic annotation. Source: Ensembl

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

cholesterol transporter activity

Inferred from electronic annotation. Source: Ensembl

glycoprotein transporter activity

Inferred from electronic annotation. Source: Ensembl

phospholipid transporter activity

Inferred from electronic annotation. Source: Ensembl

sterol-transporting ATPase activity

Inferred from electronic annotation. Source: Ensembl

toxin transporter activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 666666ATP-binding cassette sub-family G member 1
PRO_0000093385

Regions

Topological domain1 – 414414Cytoplasmic Potential
Transmembrane415 – 43319Helical; Potential
Topological domain434 – 44411Extracellular Potential
Transmembrane445 – 46521Helical; Potential
Topological domain466 – 49429Cytoplasmic Potential
Transmembrane495 – 51319Helical; Potential
Topological domain514 – 5218Extracellular Potential
Transmembrane522 – 54322Helical; Potential
Topological domain544 – 55512Cytoplasmic Potential
Transmembrane556 – 57419Helical; Potential
Topological domain575 – 63763Extracellular Potential
Transmembrane638 – 65720Helical; Potential
Topological domain658 – 6669Cytoplasmic Potential
Domain77 – 317241ABC transporter
Domain403 – 661259ABC transmembrane type-2
Nucleotide binding118 – 1258ATP Potential

Amino acid modifications

Lipidation301S-palmitoyl cysteine By similarity
Lipidation1541S-palmitoyl cysteine By similarity
Lipidation3151S-palmitoyl cysteine By similarity
Lipidation3941S-palmitoyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64343 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: EDDC6AFBD43950B6

FASTA66674,033
        10         20         30         40         50         60 
MACLMAAFSV GTAMNASSYS AAMTEPKSVC VSVDEVVSSN VDEVETDLLN GHLKKVDNNF 

        70         80         90        100        110        120 
TEAQRFSSLP RRAAVNIEFK DLSYSVPEGP WWKKKGYKTL LKGISGKFNS GELVAIMGPS 

       130        140        150        160        170        180 
GAGKSTLMNI LAGYRETGMK GAVLINGMPR DLRCFRKVSC YIMQDDMLLP HLTVQEAMMV 

       190        200        210        220        230        240 
SAHLKLQEKD EGRREMVKEI LTALGLLPCA NTRTGSLSGG QRKRLAIALE LVNNPPVMFF 

       250        260        270        280        290        300 
DEPTSGLDSA SCFQVVSLMK GLAQGGRSIV CTIHQPSAKL FELFDQLYVL SQGQCVYRGK 

       310        320        330        340        350        360 
VSNLVPYLRD LGLNCPTYHN PADFVMEVAS GEYGDQNSRL VRAVREGMCD ADYKRDLGGD 

       370        380        390        400        410        420 
TDVNPFLWHR PAEEDSASME GCHSFSASCL TQFCILFKRT FLSIMRDSVL THLRITSHIG 

       430        440        450        460        470        480 
IGLLIGLLYL GIGNEAKKVL SNSGFLFFSM LFLMFAALMP TVLTFPLEMS VFLREHLNYW 

       490        500        510        520        530        540 
YSLKAYYLAK TMADVPFQIM FPVAYCSIVY WMTSQPSDAV RFVLFAALGT MTSLVAQSLG 

       550        560        570        580        590        600 
LLIGAASTSL QVATFVGPVT AIPVLLFSGF FVSFDTIPAY LQWMSYISYV RYGFEGVILS 

       610        620        630        640        650        660 
IYGLDREDLH CDIAETCHFQ KSEAILRELD VENAKLYLDF IVLGIFFISL RLIAYFVLRY 


KIRAER 

« Hide

References

[1]"Isolation and characterization of a mammalian homolog of the Drosophila white gene."
Croop J.M., Tiller G.E., Fletcher J.A., Lux M.L., Raab E., Goldenson D., Son D., Arciniegas S., Wu R.
Gene 185:77-85(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning of a mammalian ABC transporter homologous to Drosophila white gene."
Savary S., Denizot F., Luciani M.-F., Mattei M.-G., Chimini G.
Mamm. Genome 7:673-676(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: DBA/2.
[3]"Genomic sequence and structure of the human ABCG1 (ABC8) gene."
Lorkowski S., Rust S., Engel T., Jung E., Tegelkamp K., Galinski E.A., Assmann G., Cullen P.
Biochem. Biophys. Res. Commun. 280:121-131(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Human white/murine ABC8 mRNA levels are highly induced in lipid-loaded macrophages. A transcriptional role for specific oxysterols."
Venkateswaran A., Repa J.J., Lobaccaro J.-M.A., Bronson A., Mangelsdorf D.J., Edwards P.A.
J. Biol. Chem. 275:14700-14707(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, PROBABLE FUNCTION.
[5]"Role of ABCG1 and other ABCG family members in lipid metabolism."
Schmitz G., Langmann T., Heimerl S.
J. Lipid Res. 42:1513-1520(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[6]"Physiological function of ABCG1."
Ito T.
Drug News Perspect. 16:490-492(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: differential role of LXR."
Khovidhunkit W., Moser A.H., Shigenaga J.K., Grunfeld C., Feingold K.R.
J. Lipid Res. 44:1728-1736(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DOWN-REGULATION BY ENDOTOXIN.
[8]"Characterization of palmitoylation of ATP binding cassette transporter G1: Effect on protein trafficking and function."
Gu H.M., Li G., Gao X., Berthiaume L.G., Zhang D.W.
Biochim. Biophys. Acta 1831:1067-1078(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: PALMITOYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U34920 mRNA. Translation: AAB47738.1.
Z48745 mRNA. Translation: CAA88636.1.
AF323659 mRNA. Translation: AAK27442.1.
CCDSCCDS28602.1.
RefSeqNP_033723.1. NM_009593.2.
UniGeneMm.15691.
Mm.470747.

3D structure databases

ProteinModelPortalQ64343.
SMRQ64343. Positions 2-323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid197904. 1 interaction.

PTM databases

PhosphoSiteQ64343.

Proteomic databases

MaxQBQ64343.
PaxDbQ64343.
PRIDEQ64343.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000024829; ENSMUSP00000024829; ENSMUSG00000024030.
GeneID11307.
KEGGmmu:11307.
UCSCuc008buj.2. mouse.

Organism-specific databases

CTD9619.
MGIMGI:107704. Abcg1.

Phylogenomic databases

eggNOGCOG1131.
GeneTreeENSGT00740000114855.
HOGENOMHOG000236364.
HOVERGENHBG103052.
InParanoidQ64343.
KOK05679.
OMARERICDT.
OrthoDBEOG7KWSGT.
PhylomeDBQ64343.
TreeFamTF105210.

Gene expression databases

ArrayExpressQ64343.
BgeeQ64343.
GenevestigatorQ64343.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR020064. ABCG1.
IPR027417. P-loop_NTPase.
IPR005284. Pigment_permease.
[Graphical view]
PANTHERPTHR19241:SF177. PTHR19241:SF177. 1 hit.
PfamPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00955. 3a01204. 1 hit.
PROSITEPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio278604.
PROQ64343.
SOURCESearch...

Entry information

Entry nameABCG1_MOUSE
AccessionPrimary (citable) accession number: Q64343
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot