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Q64338 (PDE1C_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C

Short name=Cam-PDE 1C
EC=3.1.4.17
Gene names
Name:Pde1c
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a high affinity for both cAMP and cGMP.

Catalytic activity

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Enzyme regulation

Type I PDE are activated by the binding of calmodulin in the presence of Ca2+.

Subunit structure

Homodimer By similarity.

Tissue specificity

Isoform 1 is highly expressed in testis. Isoform 2 is highly expressed in heart and testis, and detected at lower levels in olfactory epithelium and cerebellum.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 3 (identifier: Q64338-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q64338-1)

Also known as: PDE1C4; PDE1C5;

The sequence of this isoform differs from the canonical sequence as follows:
     651-654: VIKP → GDYG
     655-706: Missing.
Isoform 2 (identifier: Q64338-2)

Also known as: PDE1C1;

The sequence of this isoform differs from the canonical sequence as follows:
     628-631: GTKK → DPEE
     632-706: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 706706Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
PRO_0000198793

Regions

Region202 – 521320Catalytic By similarity

Sites

Active site2281Proton donor By similarity
Metal binding2321Divalent metal cation 1 By similarity
Metal binding2681Divalent metal cation 1 By similarity
Metal binding2691Divalent metal cation 1 By similarity
Metal binding2691Divalent metal cation 2 By similarity
Metal binding3761Divalent metal cation 1 By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Natural variations

Alternative sequence628 – 6314GTKK → DPEE in isoform 2.
VSP_004554
Alternative sequence632 – 70675Missing in isoform 2.
VSP_012380
Alternative sequence651 – 6544VIKP → GDYG in isoform 1.
VSP_012381
Alternative sequence655 – 70652Missing in isoform 1.
VSP_012382

Sequences

Sequence LengthMass (Da)Tools
Isoform 3 [UniParc].

Last modified January 4, 2005. Version 2.
Checksum: 1F58533E1259801F

FASTA70680,290
        10         20         30         40         50         60 
MESPTKEIEE FESNSLKHLQ PEQIEKIWLR LRGLRKYKKT SQRLRSLVKQ LERGEASVVD 

        70         80         90        100        110        120 
LKKNLEYAAT VLESVYIDET RRLLDTEDEL SDIQSDAVPS EVRDWLASTF TRQMGMMLRR 

       130        140        150        160        170        180 
SDEKPRFKSI VHAVQAGIFV ERMYRRTSNM VGLSYPPAVI DALKDVDTWS FDVFSLNEAS 

       190        200        210        220        230        240 
GDHALKFIFY ELLTRYDLIS RFKIPISALV SFVEALEVGY SKHKNPYHNL MHAADVTQTV 

       250        260        270        280        290        300 
HYLLYKTGVA NWLTELEIFA IIFSAAIHDY EHTGTTNNFH IQTRSDPAIL YNDRSVLENH 

       310        320        330        340        350        360 
HLSAAYRLLQ EDEEMNILVN LSKDDWREFR TLVIEMVMAT DMSCHFQQIK AMKTALQQPE 

       370        380        390        400        410        420 
AIEKPKALSL MLHTADISHP AKAWDLHHRW TMSLLEEFFR QGDREAELGL PFSPLCDRKS 

       430        440        450        460        470        480 
TMVAQSQVGF IDFIVEPTFT VLTDMTEKIV SPLIDESSQT GGTGQRRSSL NSINSSDAKR 

       490        500        510        520        530        540 
SGVKSSGSDG SAPINNSVIP VDYKSFKATW TEVVQINRER WRAKVPKEEK AKKEAEEKAR 

       550        560        570        580        590        600 
LAAEEKQKEM EAKSQAEQGT TSKGEKKTSG EAKSQVNGTR KGDNPRGKNS KGEKAGEKQQ 

       610        620        630        640        650        660 
NGDLKDGKNK ADKKDHSNTG NESKKTDGTK KRSHGSPAPS TSSTSRITLP VIKPPLRHFK 

       670        680        690        700 
RPAYASSSYA PSVPKKTDDH PVRYKMLDQR IKMKKIQNIS HHWNKK 

« Hide

Isoform 1 (PDE1C4) (PDE1C5) [UniParc].

Checksum: EEDFFCF64FB08F39
Show »

FASTA65474,004
Isoform 2 (PDE1C1) [UniParc].

Checksum: DE0C171B61B6F176
Show »

FASTA63171,746

References

« Hide 'large scale' references
[1]"The calmodulin-dependent phosphodiesterase gene PDE1C encodes several functionally different splice variants in a tissue-specific manner."
Yan C., Zhao A.Z., Bentley J.K., Beavo J.A.
J. Biol. Chem. 271:25699-25706(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Strain: BALB/c.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: C57BL/6J.
Tissue: Pituitary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L76947 mRNA. Translation: AAC37702.1.
L76946 mRNA. Translation: AAC37703.1.
L76944 mRNA. Translation: AAC37701.1.
AK030423 mRNA. Translation: BAC26956.1.
CCDSCCDS20170.1. [Q64338-1]
CCDS51784.1. [Q64338-3]
CCDS51785.1. [Q64338-2]
RefSeqNP_001020739.1. NM_001025568.2. [Q64338-2]
NP_001153424.1. NM_001159952.1.
NP_001153425.1. NM_001159953.1. [Q64338-3]
NP_001153427.1. NM_001159955.1. [Q64338-1]
NP_001153432.1. NM_001159960.1. [Q64338-2]
NP_035184.1. NM_011054.4. [Q64338-1]
UniGeneMm.5145.

3D structure databases

ProteinModelPortalQ64338.
SMRQ64338. Positions 151-450.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ64338.

Proteomic databases

PaxDbQ64338.
PRIDEQ64338.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000044505; ENSMUSP00000046601; ENSMUSG00000004347. [Q64338-3]
ENSMUST00000114327; ENSMUSP00000109966; ENSMUSG00000004347. [Q64338-1]
ENSMUST00000164752; ENSMUSP00000129185; ENSMUSG00000004347. [Q64338-2]
ENSMUST00000166102; ENSMUSP00000131350; ENSMUSG00000004347. [Q64338-2]
ENSMUST00000168944; ENSMUSP00000128364; ENSMUSG00000004347. [Q64338-1]
GeneID18575.
KEGGmmu:18575.
UCSCuc009caz.2. mouse. [Q64338-3]
uc009cbb.2. mouse. [Q64338-1]

Organism-specific databases

CTD5137.
MGIMGI:108413. Pde1c.

Phylogenomic databases

eggNOGNOG139098.
GeneTreeENSGT00660000095451.
HOGENOMHOG000231888.
HOVERGENHBG056120.
InParanoidQ64338.
KOK13755.
OMANPRGKNS.
OrthoDBEOG7X9G6J.
PhylomeDBQ64338.
TreeFamTF314638.

Gene expression databases

ArrayExpressQ64338.
BgeeQ64338.
CleanExMM_PDE1C.
GenevestigatorQ64338.

Family and domain databases

Gene3D1.10.1300.10. 2 hits.
InterProIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013706. PDEase_N.
[Graphical view]
PfamPF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294428.
PROQ64338.
SOURCESearch...

Entry information

Entry namePDE1C_MOUSE
AccessionPrimary (citable) accession number: Q64338
Secondary accession number(s): Q62045, Q8BSV6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 4, 2005
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot