Q64338 (PDE1C_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
Short name=Cam-PDE 1C
EC=3.1.4.17

Gene names

Name:

Pde1c

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a high affinity for both cAMP and cGMP.
Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Enzyme regulation	Type I PDE are activated by the binding of calmodulin in the presence of Ca2+.
Subunit structure	Homodimer By similarity.
Tissue specificity	Isoform 1 is highly expressed in testis. Isoform 2 is highly expressed in heart and testis, and detected at lower levels in olfactory epithelium and cerebellum.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Calmodulin-binding Metal-binding cAMP cGMP
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to calcium ion Inferred from sequence orthology PubMed 7568196. Source: MGI sensory perception of smell Inferred from mutant phenotype PubMed 19305400. Source: MGI signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cilium Inferred from direct assay PubMed 19305400. Source: MGI
Molecular_function	calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity Inferred from sequence orthology PubMed 7568196. Source: MGI calmodulin-dependent cyclic-nucleotide phosphodiesterase activity Inferred from sequence orthology PubMed 7568196. Source: MGI metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 3 (identifier: Q64338-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q64338-1)

Also known as: PDE1C4; PDE1C5;

The sequence of this isoform differs from the canonical sequence as follows:
     651-654: VIKP → GDYG
     655-706: Missing.

Isoform 2 (identifier: Q64338-2)

Also known as: PDE1C1;

The sequence of this isoform differs from the canonical sequence as follows:
     628-631: GTKK → DPEE
     632-706: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 706	706	Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C		PRO_0000198793
Regions
	Region	202 – 521	320	Catalytic By similarity
Sites
	Active site	228	1	Proton donor By similarity
	Metal binding	232	1	Divalent metal cation 1 By similarity
	Metal binding	268	1	Divalent metal cation 1 By similarity
	Metal binding	269	1	Divalent metal cation 1 By similarity
	Metal binding	269	1	Divalent metal cation 2 By similarity
	Metal binding	376	1	Divalent metal cation 1 By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
Natural variations
	Alternative sequence	628 – 631	4	GTKK → DPEE in isoform 2.		VSP_004554
	Alternative sequence	632 – 706	75	Missing in isoform 2.		VSP_012380
	Alternative sequence	651 – 654	4	VIKP → GDYG in isoform 1.		VSP_012381
	Alternative sequence	655 – 706	52	Missing in isoform 1.		VSP_012382

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: 1F58533E1259801F	FASTA	706	80,290
	10 20 30 40 50 60 MESPTKEIEE FESNSLKHLQ PEQIEKIWLR LRGLRKYKKT SQRLRSLVKQ LERGEASVVD 70 80 90 100 110 120 LKKNLEYAAT VLESVYIDET RRLLDTEDEL SDIQSDAVPS EVRDWLASTF TRQMGMMLRR 130 140 150 160 170 180 SDEKPRFKSI VHAVQAGIFV ERMYRRTSNM VGLSYPPAVI DALKDVDTWS FDVFSLNEAS 190 200 210 220 230 240 GDHALKFIFY ELLTRYDLIS RFKIPISALV SFVEALEVGY SKHKNPYHNL MHAADVTQTV 250 260 270 280 290 300 HYLLYKTGVA NWLTELEIFA IIFSAAIHDY EHTGTTNNFH IQTRSDPAIL YNDRSVLENH 310 320 330 340 350 360 HLSAAYRLLQ EDEEMNILVN LSKDDWREFR TLVIEMVMAT DMSCHFQQIK AMKTALQQPE 370 380 390 400 410 420 AIEKPKALSL MLHTADISHP AKAWDLHHRW TMSLLEEFFR QGDREAELGL PFSPLCDRKS 430 440 450 460 470 480 TMVAQSQVGF IDFIVEPTFT VLTDMTEKIV SPLIDESSQT GGTGQRRSSL NSINSSDAKR 490 500 510 520 530 540 SGVKSSGSDG SAPINNSVIP VDYKSFKATW TEVVQINRER WRAKVPKEEK AKKEAEEKAR 550 560 570 580 590 600 LAAEEKQKEM EAKSQAEQGT TSKGEKKTSG EAKSQVNGTR KGDNPRGKNS KGEKAGEKQQ 610 620 630 640 650 660 NGDLKDGKNK ADKKDHSNTG NESKKTDGTK KRSHGSPAPS TSSTSRITLP VIKPPLRHFK 670 680 690 700 RPAYASSSYA PSVPKKTDDH PVRYKMLDQR IKMKKIQNIS HHWNKK
	Isoform 1 (PDE1C4) (PDE1C5) [UniParc]. Checksum: EEDFFCF64FB08F39	FASTA	654	74,004
	10 20 30 40 50 60 MESPTKEIEE FESNSLKHLQ PEQIEKIWLR LRGLRKYKKT SQRLRSLVKQ LERGEASVVD 70 80 90 100 110 120 LKKNLEYAAT VLESVYIDET RRLLDTEDEL SDIQSDAVPS EVRDWLASTF TRQMGMMLRR 130 140 150 160 170 180 SDEKPRFKSI VHAVQAGIFV ERMYRRTSNM VGLSYPPAVI DALKDVDTWS FDVFSLNEAS 190 200 210 220 230 240 GDHALKFIFY ELLTRYDLIS RFKIPISALV SFVEALEVGY SKHKNPYHNL MHAADVTQTV 250 260 270 280 290 300 HYLLYKTGVA NWLTELEIFA IIFSAAIHDY EHTGTTNNFH IQTRSDPAIL YNDRSVLENH 310 320 330 340 350 360 HLSAAYRLLQ EDEEMNILVN LSKDDWREFR TLVIEMVMAT DMSCHFQQIK AMKTALQQPE 370 380 390 400 410 420 AIEKPKALSL MLHTADISHP AKAWDLHHRW TMSLLEEFFR QGDREAELGL PFSPLCDRKS 430 440 450 460 470 480 TMVAQSQVGF IDFIVEPTFT VLTDMTEKIV SPLIDESSQT GGTGQRRSSL NSINSSDAKR 490 500 510 520 530 540 SGVKSSGSDG SAPINNSVIP VDYKSFKATW TEVVQINRER WRAKVPKEEK AKKEAEEKAR 550 560 570 580 590 600 LAAEEKQKEM EAKSQAEQGT TSKGEKKTSG EAKSQVNGTR KGDNPRGKNS KGEKAGEKQQ 610 620 630 640 650 NGDLKDGKNK ADKKDHSNTG NESKKTDGTK KRSHGSPAPS TSSTSRITLP GDYG
	Isoform 2 (PDE1C1) [UniParc]. Checksum: DE0C171B61B6F176	FASTA	631	71,746
	10 20 30 40 50 60 MESPTKEIEE FESNSLKHLQ PEQIEKIWLR LRGLRKYKKT SQRLRSLVKQ LERGEASVVD 70 80 90 100 110 120 LKKNLEYAAT VLESVYIDET RRLLDTEDEL SDIQSDAVPS EVRDWLASTF TRQMGMMLRR 130 140 150 160 170 180 SDEKPRFKSI VHAVQAGIFV ERMYRRTSNM VGLSYPPAVI DALKDVDTWS FDVFSLNEAS 190 200 210 220 230 240 GDHALKFIFY ELLTRYDLIS RFKIPISALV SFVEALEVGY SKHKNPYHNL MHAADVTQTV 250 260 270 280 290 300 HYLLYKTGVA NWLTELEIFA IIFSAAIHDY EHTGTTNNFH IQTRSDPAIL YNDRSVLENH 310 320 330 340 350 360 HLSAAYRLLQ EDEEMNILVN LSKDDWREFR TLVIEMVMAT DMSCHFQQIK AMKTALQQPE 370 380 390 400 410 420 AIEKPKALSL MLHTADISHP AKAWDLHHRW TMSLLEEFFR QGDREAELGL PFSPLCDRKS 430 440 450 460 470 480 TMVAQSQVGF IDFIVEPTFT VLTDMTEKIV SPLIDESSQT GGTGQRRSSL NSINSSDAKR 490 500 510 520 530 540 SGVKSSGSDG SAPINNSVIP VDYKSFKATW TEVVQINRER WRAKVPKEEK AKKEAEEKAR 550 560 570 580 590 600 LAAEEKQKEM EAKSQAEQGT TSKGEKKTSG EAKSQVNGTR KGDNPRGKNS KGEKAGEKQQ 610 620 630 NGDLKDGKNK ADKKDHSNTG NESKKTDDPE E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The calmodulin-dependent phosphodiesterase gene PDE1C encodes several functionally different splice variants in a tissue-specific manner." Yan C., Zhao A.Z., Bentley J.K., Beavo J.A. J. Biol. Chem. 271:25699-25706(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: BALB/c. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6J. Tissue: Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L76947 mRNA. Translation: AAC37702.1. L76946 mRNA. Translation: AAC37703.1. L76944 mRNA. Translation: AAC37701.1. AK030423 mRNA. Translation: BAC26956.1.
IPI	IPI00133376. IPI00225618. IPI00466650.
RefSeq	NP_001020739.1. NM_001025568.2. NP_001153424.1. NM_001159952.1. NP_001153425.1. NM_001159953.1. NP_001153427.1. NM_001159955.1. NP_001153432.1. NM_001159960.1. NP_035184.1. NM_011054.4.
UniGene	Mm.5145.
3D structure databases
ProteinModelPortal	Q64338.
SMR	Q64338. Positions 151-450.
ModBase	Search...
PTM databases
PhosphoSite	Q64338.
Proteomic databases
PaxDb	Q64338.
PRIDE	Q64338.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000044505; ENSMUSP00000046601; ENSMUSG00000004347. ENSMUST00000114327; ENSMUSP00000109966; ENSMUSG00000004347. ENSMUST00000164752; ENSMUSP00000129185; ENSMUSG00000004347. ENSMUST00000166102; ENSMUSP00000131350; ENSMUSG00000004347. ENSMUST00000168944; ENSMUSP00000128364; ENSMUSG00000004347.
GeneID	18575.
KEGG	mmu:18575.
UCSC	uc009caz.2. mouse. uc009cbb.2. mouse.
Organism-specific databases
CTD	5137.
MGI	MGI:108413. Pde1c.
Phylogenomic databases
eggNOG	NOG139098.
GeneTree	ENSGT00660000095451.
HOGENOM	HOG000231888.
HOVERGEN	HBG056120.
InParanoid	Q64338.
KO	K13755.
OMA	KDHSNIG.
OrthoDB	EOG4NZTSR.
Gene expression databases
ArrayExpress	Q64338.
Bgee	Q64338.
CleanEx	MM_PDE1C.
Genevestigator	Q64338.
GermOnline	ENSMUSG00000004347. Mus musculus.
Family and domain databases
Gene3D	1.10.1300.10. 2 hits.
InterPro	IPR003607. HD/PDEase_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. IPR013706. PDEase_N. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. PF08499. PDEase_I_N. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	294428.
SOURCE	Search...

Entry information

Entry name

PDE1C_MOUSE

Accession

Primary (citable) accession number: Q64338
Secondary accession number(s): Q62045, Q8BSV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program