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Protein

Synapsin-2

Gene

Syn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons.1 Publication

GO - Molecular functioni

  • ATP binding Source: InterPro
  • catalytic activity Source: InterPro
  • protein dimerization activity Source: MGI

GO - Biological processi

  • neurotransmitter secretion Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Synapsin-2
Alternative name(s):
Synapsin II
Gene namesi
Name:Syn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:103020. Syn2.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • myelin sheath Source: UniProtKB
  • neuronal postsynaptic density Source: MGI
  • synapse Source: MGI
  • synaptic vesicle membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 586586Synapsin-2PRO_0000183022Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101Phosphoserine; by PKA and CaMK1By similarity
Modified residuei426 – 4261Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation at Ser-10 dissociates synapsins from synaptic vesicles (By similarity). Phosphorylation at Ser-426 by MAPK1/ERK2 and/or MAPK3/ERK1 may play a role in noradrenaline secretion by sympathetic neurons.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ64332.
PaxDbiQ64332.
PRIDEiQ64332.

PTM databases

PhosphoSiteiQ64332.

Expressioni

Tissue specificityi

Expressed exclusively in neuronal cells. Isoform IIb is enriched in sympathetic cervical ganglion.1 Publication

Gene expression databases

BgeeiQ64332.
CleanExiMM_SYN2.
GenevestigatoriQ64332.

Interactioni

Subunit structurei

Interacts with CAPON.By similarity

Protein-protein interaction databases

BioGridi203601. 5 interactions.
IntActiQ64332. 8 interactions.
MINTiMINT-215012.

Structurei

3D structure databases

ProteinModelPortaliQ64332.
SMRiQ64332. Positions 113-420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2929AAdd
BLAST
Regioni33 – 11381B; linkerAdd
BLAST
Regioni114 – 421308C; actin-binding and synaptic-vesicle bindingAdd
BLAST
Regioni422 – 45837G; Pro-rich linkerAdd
BLAST
Regioni459 – 53779H; Pro/Ser-rich linkerAdd
BLAST
Regioni538 – 58649EAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi486 – 49914Poly-SerAdd
BLAST
Compositional biasi515 – 5206Poly-Ser

Domaini

The A region binds phospholipids with a preference for negatively charged species.By similarity

Sequence similaritiesi

Belongs to the synapsin family.Curated

Phylogenomic databases

eggNOGiNOG284201.
GeneTreeiENSGT00530000063319.
HOGENOMiHOG000231323.
HOVERGENiHBG016354.
InParanoidiQ64332.
OMAiVINKMNQ.
OrthoDBiEOG793B7G.
PhylomeDBiQ64332.
TreeFamiTF319919.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR001359. Synapsin.
IPR020898. Synapsin_ATP-bd_dom.
IPR019735. Synapsin_CS.
IPR019736. Synapsin_P_site.
IPR020897. Synapsin_pre-ATP-grasp_dom.
[Graphical view]
PANTHERiPTHR10841. PTHR10841. 1 hit.
PfamiPF02078. Synapsin. 1 hit.
PF02750. Synapsin_C. 1 hit.
PF10581. Synapsin_N. 1 hit.
[Graphical view]
PRINTSiPR01368. SYNAPSIN.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform IIa (identifier: Q64332-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMNFLRRRLS DSSFIANLPN GYMTDLQRPE PQQPPPAPGP GAATASAATS
60 70 80 90 100
AASPGPERRP PPAQAPAPQP APQPAPTPSV GSSFFSSLSQ AVKQTAASAG
110 120 130 140 150
LVDAPAPSAA SRKAKVLLVV DEPHTDWAKC FRGKKILGDY DIKVEQAEFS
160 170 180 190 200
ELNLVAHADG TYAVDMQVLR NGTKVVRSFR PDFVLIRQHA FGMAENEDFR
210 220 230 240 250
HLVIGMQYAG LPSINSLESI YNFCDKPWVF AQMVAIFKTL GGEKFPLIEQ
260 270 280 290 300
TYYPNHREML TLPTFPVVVK IGHAHSGMGK VKVENHYDFQ DIASVVALTQ
310 320 330 340 350
TYATAEPFID AKYDIRVQKI GNNYKAYMRT SISGNWKTNT GSAMLEQIAM
360 370 380 390 400
SDRYKLWVDA CSEMFGGLDI CAVKAVHGKD GKDYIFEVMD CSMPLIGEHQ
410 420 430 440 450
VEDRQLITDL VISKMNQLLS RTPALSPQRP LTTQQPQSGT LKEPDSSKTP
460 470 480 490 500
PQRPPPQGGP GQPQGMQPPG KVLPPRRLPS GPSLPSSSSS SSSSSSSSSA
510 520 530 540 550
PQRPGGPTTT HGDASSSSNS LAEAQAPQAA PAQKPQPHPQ LNKSQSLTNA
560 570 580
FSFSESSFFR SSANEDEAKA ETIRSLRKSF ASLFSD
Length:586
Mass (Da):63,373
Last modified:August 30, 2005 - v2
Checksum:i2B1B6A7453E286FC
GO
Isoform IIb (identifier: Q64332-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     459-479: GPGQPQGMQPPGKVLPPRRLP → CLQYILDCNGIAVGPKQVQAS
     480-586: Missing.

Show »
Length:479
Mass (Da):52,452
Checksum:i21EF91B466D08BB5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei459 – 47921GPGQP…PRRLP → CLQYILDCNGIAVGPKQVQA S in isoform IIb. 1 PublicationVSP_015203Add
BLAST
Alternative sequencei480 – 586107Missing in isoform IIb. 1 PublicationVSP_015204Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096867 mRNA. Translation: AAC72966.1.
L32026 Genomic DNA. Translation: AAA79964.1.
BC066004 mRNA. Translation: AAH66004.1.
BC085129 mRNA. Translation: AAH85129.1.
CCDSiCCDS51874.1. [Q64332-1]
CCDS51875.1. [Q64332-2]
PIRiI61260.
RefSeqiNP_001104485.1. NM_001111015.1. [Q64332-1]
NP_038709.1. NM_013681.3. [Q64332-2]
UniGeneiMm.441431.
Mm.466323.

Genome annotation databases

EnsembliENSMUST00000009538; ENSMUSP00000009538; ENSMUSG00000009394. [Q64332-1]
ENSMUST00000169345; ENSMUSP00000133121; ENSMUSG00000009394. [Q64332-2]
GeneIDi20965.
KEGGimmu:20965.
UCSCiuc009dim.2. mouse. [Q64332-2]
uc009din.2. mouse. [Q64332-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096867 mRNA. Translation: AAC72966.1.
L32026 Genomic DNA. Translation: AAA79964.1.
BC066004 mRNA. Translation: AAH66004.1.
BC085129 mRNA. Translation: AAH85129.1.
CCDSiCCDS51874.1. [Q64332-1]
CCDS51875.1. [Q64332-2]
PIRiI61260.
RefSeqiNP_001104485.1. NM_001111015.1. [Q64332-1]
NP_038709.1. NM_013681.3. [Q64332-2]
UniGeneiMm.441431.
Mm.466323.

3D structure databases

ProteinModelPortaliQ64332.
SMRiQ64332. Positions 113-420.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203601. 5 interactions.
IntActiQ64332. 8 interactions.
MINTiMINT-215012.

PTM databases

PhosphoSiteiQ64332.

Proteomic databases

MaxQBiQ64332.
PaxDbiQ64332.
PRIDEiQ64332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000009538; ENSMUSP00000009538; ENSMUSG00000009394. [Q64332-1]
ENSMUST00000169345; ENSMUSP00000133121; ENSMUSG00000009394. [Q64332-2]
GeneIDi20965.
KEGGimmu:20965.
UCSCiuc009dim.2. mouse. [Q64332-2]
uc009din.2. mouse. [Q64332-1]

Organism-specific databases

CTDi6854.
MGIiMGI:103020. Syn2.

Phylogenomic databases

eggNOGiNOG284201.
GeneTreeiENSGT00530000063319.
HOGENOMiHOG000231323.
HOVERGENiHBG016354.
InParanoidiQ64332.
OMAiVINKMNQ.
OrthoDBiEOG793B7G.
PhylomeDBiQ64332.
TreeFamiTF319919.

Miscellaneous databases

ChiTaRSiSyn2. mouse.
NextBioi299926.
PROiQ64332.
SOURCEiSearch...

Gene expression databases

BgeeiQ64332.
CleanExiMM_SYN2.
GenevestigatoriQ64332.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR001359. Synapsin.
IPR020898. Synapsin_ATP-bd_dom.
IPR019735. Synapsin_CS.
IPR019736. Synapsin_P_site.
IPR020897. Synapsin_pre-ATP-grasp_dom.
[Graphical view]
PANTHERiPTHR10841. PTHR10841. 1 hit.
PfamiPF02078. Synapsin. 1 hit.
PF02750. Synapsin_C. 1 hit.
PF10581. Synapsin_N. 1 hit.
[Graphical view]
PRINTSiPR01368. SYNAPSIN.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of mouse synapsin IIb."
    Han S.J.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IIB).
    Strain: H129.
    Tissue: Brain.
  2. "Neuron-specific expression of the synapsin II gene is directed by a specific core promoter and upstream regulatory elements."
    Chin L.S., Li L., Greengard P.
    J. Biol. Chem. 269:18507-18513(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-126, TISSUE SPECIFICITY.
    Strain: 129/Sv.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-586 (ISOFORM IIA).
    Strain: C57BL/6.
    Tissue: Brain.
  4. Lubec G., Klug S., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 59-112; 116-129; 136-143; 178-213; 245-270; 338-353 AND 405-414, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.
  5. "Short-chain fatty acid receptor GPR41-mediated activation of sympathetic neurons involves synapsin 2b phosphorylation."
    Inoue D., Kimura I., Wakabayashi M., Tsumoto H., Ozawa K., Hara T., Takei Y., Hirasawa A., Ishihama Y., Tsujimoto G.
    FEBS Lett. 586:1547-1554(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT SER-426, TISSUE SPECIFICITY (ISOFORM IIB).

Entry informationi

Entry nameiSYN2_MOUSE
AccessioniPrimary (citable) accession number: Q64332
Secondary accession number(s): Q6NZR0, Q9QWV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 30, 2005
Last modified: February 4, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.