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Q64323 (PIGA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
Alternative name(s):
GlcNAc-PI synthesis protein
Phosphatidylinositol-glycan biosynthesis class A protein
Short name=PIG-A
Gene names
Name:Piga
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.

Pathway

Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.

Subunit structure

Associates with PIGC, PIGH, PIGP, PIGQ and DPM2. The latter is not essential for activity. Interacts directly with PIGY By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein.

Sequence similarities

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 485485N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
PRO_0000080327

Regions

Topological domain1 – 422422Cytoplasmic Potential
Transmembrane423 – 44321Helical; Potential
Topological domain444 – 48542Lumenal Potential

Amino acid modifications

Modified residue211Phosphoserine By similarity

Experimental info

Sequence conflict1071F → I in BAC27792. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q64323 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 12141BB48A71A03A

FASTA48554,469
        10         20         30         40         50         60 
MANRRGGGQG QPPSVSPSPG SSGNLSDDRT CTHNICMVSD FFYPNMGGVE SHIYQLSQCL 

        70         80         90        100        110        120 
IERGHKVITV THAYGNRKGV RYLTNGLKVY YLPLRVMYNQ STATTLFHSL PLLRYIFVRE 

       130        140        150        160        170        180 
RITIIHSHSS FSAMAHDALF HAKTMGLQTV FTDHSLFGFA DVSSVLTNKL LTVSLCDTNH 

       190        200        210        220        230        240 
IICVSYTSKE NTVLRAALNP EIVSVIPNAV DPTDFTPDPF RRHDSVITVV VVSRLVYRKG 

       250        260        270        280        290        300 
TDLLSGIIPE LCQKYQELHF LIGGEGPKRI ILEEVRERYQ LHDRVQLLGA LEHKDVRNVL 

       310        320        330        340        350        360 
VQGHIFLNTS LTEAFCMAIV EAASCGLQVV STKVGGIPEV LPESLIILCE PSVKSLCDGL 

       370        380        390        400        410        420 
EKAIFQVKSG TLPAPENIHN VVKTFYTWRN VAERTEKVYE RVSKETVLPM HKRLDRLISH 

       430        440        450        460        470        480 
CGPVTGYMFA LLAVLSYLFL IFLQWMTPDS FIDVAIDATG PRRAWTHQWP RDKKRDENDK 


ISQSR

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of murine pig-a, a gene for GPI-anchor biosynthesis, and demonstration of interspecies conservation of its structure, function, and genetic locus."
Kawagoe K., Takeda J., Endo Y., Kinoshita T.
Genomics 23:566-574(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: 129S/V.
[2]"Cloning and characterization of the mouse PIG-A gene."
Yu J., Nagarajan S., Liu J., Young N., Medof M.E.
Biochim. Biophys. Acta 1255:344-350(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D31863 Genomic DNA. Translation: BAA06663.1.
D26047 mRNA. Translation: BAA05047.1.
S78188 mRNA. Translation: AAP32009.1.
AK032283 mRNA. Translation: BAC27792.1.
IPIIPI00133314.
PIRA55731.
I52484.
RefSeqNP_035211.2. NM_011081.2.
UniGeneMm.3781.
Mm.401070.

3D structure databases

ProteinModelPortalQ64323.
SMRQ64323. Positions 206-414.
ModBaseSearch...

Protein family/group databases

CAZyGT4. Glycosyltransferase Family 4.

PTM databases

PhosphoSiteQ64323.

Proteomic databases

PaxDbQ64323.
PRIDEQ64323.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033754; ENSMUSP00000033754; ENSMUSG00000031381.
ENSMUST00000112255; ENSMUSP00000107874; ENSMUSG00000031381.
GeneID18700.
KEGGmmu:18700.

Organism-specific databases

CTD5277.
MGIMGI:99461. Piga.

Phylogenomic databases

eggNOGCOG0438.
GeneTreeENSGT00390000014405.
HOGENOMHOG000203293.
HOVERGENHBG008198.
InParanoidQ64323.
KOK03857.
OrthoDBEOG4GF3F7.

Enzyme and pathway databases

UniPathwayUPA00196.

Gene expression databases

ArrayExpressQ64323.
BgeeQ64323.
GenevestigatorQ64323.
GermOnlineENSMUSG00000031381. Mus musculus.

Family and domain databases

InterProIPR001296. Glyco_trans_1.
IPR013234. PIGA_GPI_anchor_biosynthesis.
[Graphical view]
PfamPF00534. Glycos_transf_1. 1 hit.
PF08288. PIGA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294747.
SOURCESearch...

Entry information

Entry namePIGA_MOUSE
AccessionPrimary (citable) accession number: Q64323
Secondary accession number(s): Q6LD71, Q8CCQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: November 1, 1996
Last modified: May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents