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Q64323

- PIGA_MOUSE

UniProt

Q64323 - PIGA_MOUSE

Protein

N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein

Gene

Piga

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.

    Pathwayi

    GO - Molecular functioni

    1. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

    GO - Biological processi

    1. GPI anchor biosynthetic process Source: MGI

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    GPI-anchor biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00196.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
    Alternative name(s):
    GlcNAc-PI synthesis protein
    Phosphatidylinositol-glycan biosynthesis class A protein
    Short name:
    PIG-A
    Gene namesi
    Name:Piga
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:99461. Piga.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: HGNC
    2. glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex Source: HGNC
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 485485N-acetylglucosaminyl-phosphatidylinositol biosynthetic proteinPRO_0000080327Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei21 – 211PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ64323.
    PRIDEiQ64323.

    PTM databases

    PhosphoSiteiQ64323.

    Expressioni

    Gene expression databases

    ArrayExpressiQ64323.
    BgeeiQ64323.
    GenevestigatoriQ64323.

    Interactioni

    Subunit structurei

    Associates with PIGC, PIGH, PIGP, PIGQ and DPM2. The latter is not essential for activity. Interacts directly with PIGY By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ64323.
    SMRiQ64323. Positions 206-414.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 422422CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini444 – 48542LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei423 – 44321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0438.
    GeneTreeiENSGT00390000014405.
    HOGENOMiHOG000203293.
    HOVERGENiHBG008198.
    InParanoidiQ64323.
    KOiK03857.
    OMAiHTGRENT.
    OrthoDBiEOG7DC24J.
    PhylomeDBiQ64323.
    TreeFamiTF105675.

    Family and domain databases

    InterProiIPR001296. Glyco_trans_1.
    IPR013234. PIGA_GPI_anchor_biosynthesis.
    [Graphical view]
    PfamiPF00534. Glycos_transf_1. 1 hit.
    PF08288. PIGA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64323-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANRRGGGQG QPPSVSPSPG SSGNLSDDRT CTHNICMVSD FFYPNMGGVE    50
    SHIYQLSQCL IERGHKVITV THAYGNRKGV RYLTNGLKVY YLPLRVMYNQ 100
    STATTLFHSL PLLRYIFVRE RITIIHSHSS FSAMAHDALF HAKTMGLQTV 150
    FTDHSLFGFA DVSSVLTNKL LTVSLCDTNH IICVSYTSKE NTVLRAALNP 200
    EIVSVIPNAV DPTDFTPDPF RRHDSVITVV VVSRLVYRKG TDLLSGIIPE 250
    LCQKYQELHF LIGGEGPKRI ILEEVRERYQ LHDRVQLLGA LEHKDVRNVL 300
    VQGHIFLNTS LTEAFCMAIV EAASCGLQVV STKVGGIPEV LPESLIILCE 350
    PSVKSLCDGL EKAIFQVKSG TLPAPENIHN VVKTFYTWRN VAERTEKVYE 400
    RVSKETVLPM HKRLDRLISH CGPVTGYMFA LLAVLSYLFL IFLQWMTPDS 450
    FIDVAIDATG PRRAWTHQWP RDKKRDENDK ISQSR 485
    Length:485
    Mass (Da):54,469
    Last modified:November 1, 1996 - v1
    Checksum:i12141BB48A71A03A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti107 – 1071F → I in BAC27792. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31863 Genomic DNA. Translation: BAA06663.1.
    D26047 mRNA. Translation: BAA05047.1.
    S78188 mRNA. Translation: AAP32009.1.
    AK032283 mRNA. Translation: BAC27792.1.
    CCDSiCCDS30522.1.
    PIRiA55731.
    I52484.
    RefSeqiNP_035211.2. NM_011081.2.
    UniGeneiMm.3781.
    Mm.401070.

    Genome annotation databases

    EnsembliENSMUST00000033754; ENSMUSP00000033754; ENSMUSG00000031381.
    ENSMUST00000112255; ENSMUSP00000107874; ENSMUSG00000031381.
    GeneIDi18700.
    KEGGimmu:18700.
    UCSCiuc009uvo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31863 Genomic DNA. Translation: BAA06663.1 .
    D26047 mRNA. Translation: BAA05047.1 .
    S78188 mRNA. Translation: AAP32009.1 .
    AK032283 mRNA. Translation: BAC27792.1 .
    CCDSi CCDS30522.1.
    PIRi A55731.
    I52484.
    RefSeqi NP_035211.2. NM_011081.2.
    UniGenei Mm.3781.
    Mm.401070.

    3D structure databases

    ProteinModelPortali Q64323.
    SMRi Q64323. Positions 206-414.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT4. Glycosyltransferase Family 4.

    PTM databases

    PhosphoSitei Q64323.

    Proteomic databases

    PaxDbi Q64323.
    PRIDEi Q64323.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033754 ; ENSMUSP00000033754 ; ENSMUSG00000031381 .
    ENSMUST00000112255 ; ENSMUSP00000107874 ; ENSMUSG00000031381 .
    GeneIDi 18700.
    KEGGi mmu:18700.
    UCSCi uc009uvo.1. mouse.

    Organism-specific databases

    CTDi 5277.
    MGIi MGI:99461. Piga.

    Phylogenomic databases

    eggNOGi COG0438.
    GeneTreei ENSGT00390000014405.
    HOGENOMi HOG000203293.
    HOVERGENi HBG008198.
    InParanoidi Q64323.
    KOi K03857.
    OMAi HTGRENT.
    OrthoDBi EOG7DC24J.
    PhylomeDBi Q64323.
    TreeFami TF105675.

    Enzyme and pathway databases

    UniPathwayi UPA00196 .

    Miscellaneous databases

    NextBioi 294747.
    PROi Q64323.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64323.
    Bgeei Q64323.
    Genevestigatori Q64323.

    Family and domain databases

    InterProi IPR001296. Glyco_trans_1.
    IPR013234. PIGA_GPI_anchor_biosynthesis.
    [Graphical view ]
    Pfami PF00534. Glycos_transf_1. 1 hit.
    PF08288. PIGA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of murine pig-a, a gene for GPI-anchor biosynthesis, and demonstration of interspecies conservation of its structure, function, and genetic locus."
      Kawagoe K., Takeda J., Endo Y., Kinoshita T.
      Genomics 23:566-574(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129S/V.
    2. "Cloning and characterization of the mouse PIG-A gene."
      Yu J., Nagarajan S., Liu J., Young N., Medof M.E.
      Biochim. Biophys. Acta 1255:344-350(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Brain.

    Entry informationi

    Entry nameiPIGA_MOUSE
    AccessioniPrimary (citable) accession number: Q64323
    Secondary accession number(s): Q6LD71, Q8CCQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 25, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3