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Q64323

- PIGA_MOUSE

UniProt

Q64323 - PIGA_MOUSE

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Protein

N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein

Gene

Piga

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.

Pathwayi

GO - Molecular functioni

  1. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  1. GPI anchor biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

ReactomeiREACT_243945. Synthesis of glycosylphosphatidylinositol (GPI).
UniPathwayiUPA00196.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
Alternative name(s):
GlcNAc-PI synthesis protein
Phosphatidylinositol-glycan biosynthesis class A protein
Short name:
PIG-A
Gene namesi
Name:Piga
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:99461. Piga.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 422422CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei423 – 44321HelicalSequence AnalysisAdd
BLAST
Topological domaini444 – 48542LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: HGNC
  2. glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex Source: HGNC
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485N-acetylglucosaminyl-phosphatidylinositol biosynthetic proteinPRO_0000080327Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei21 – 211PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64323.
PRIDEiQ64323.

PTM databases

PhosphoSiteiQ64323.

Expressioni

Gene expression databases

BgeeiQ64323.
ExpressionAtlasiQ64323. baseline and differential.
GenevestigatoriQ64323.

Interactioni

Subunit structurei

Associates with PIGC, PIGH, PIGP, PIGQ and DPM2. The latter is not essential for activity. Interacts directly with PIGY (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ64323.
SMRiQ64323. Positions 187-414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000014405.
HOGENOMiHOG000203293.
HOVERGENiHBG008198.
InParanoidiQ64323.
KOiK03857.
OMAiHTGRENT.
OrthoDBiEOG7DC24J.
PhylomeDBiQ64323.
TreeFamiTF105675.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR013234. PIGA_GPI_anchor_biosynthesis.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF08288. PIGA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64323-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANRRGGGQG QPPSVSPSPG SSGNLSDDRT CTHNICMVSD FFYPNMGGVE
60 70 80 90 100
SHIYQLSQCL IERGHKVITV THAYGNRKGV RYLTNGLKVY YLPLRVMYNQ
110 120 130 140 150
STATTLFHSL PLLRYIFVRE RITIIHSHSS FSAMAHDALF HAKTMGLQTV
160 170 180 190 200
FTDHSLFGFA DVSSVLTNKL LTVSLCDTNH IICVSYTSKE NTVLRAALNP
210 220 230 240 250
EIVSVIPNAV DPTDFTPDPF RRHDSVITVV VVSRLVYRKG TDLLSGIIPE
260 270 280 290 300
LCQKYQELHF LIGGEGPKRI ILEEVRERYQ LHDRVQLLGA LEHKDVRNVL
310 320 330 340 350
VQGHIFLNTS LTEAFCMAIV EAASCGLQVV STKVGGIPEV LPESLIILCE
360 370 380 390 400
PSVKSLCDGL EKAIFQVKSG TLPAPENIHN VVKTFYTWRN VAERTEKVYE
410 420 430 440 450
RVSKETVLPM HKRLDRLISH CGPVTGYMFA LLAVLSYLFL IFLQWMTPDS
460 470 480
FIDVAIDATG PRRAWTHQWP RDKKRDENDK ISQSR
Length:485
Mass (Da):54,469
Last modified:November 1, 1996 - v1
Checksum:i12141BB48A71A03A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti107 – 1071F → I in BAC27792. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31863 Genomic DNA. Translation: BAA06663.1.
D26047 mRNA. Translation: BAA05047.1.
S78188 mRNA. Translation: AAP32009.1.
AK032283 mRNA. Translation: BAC27792.1.
CCDSiCCDS30522.1.
PIRiA55731.
I52484.
RefSeqiNP_035211.2. NM_011081.2.
UniGeneiMm.3781.
Mm.401070.

Genome annotation databases

EnsembliENSMUST00000033754; ENSMUSP00000033754; ENSMUSG00000031381.
ENSMUST00000112255; ENSMUSP00000107874; ENSMUSG00000031381.
GeneIDi18700.
KEGGimmu:18700.
UCSCiuc009uvo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31863 Genomic DNA. Translation: BAA06663.1 .
D26047 mRNA. Translation: BAA05047.1 .
S78188 mRNA. Translation: AAP32009.1 .
AK032283 mRNA. Translation: BAC27792.1 .
CCDSi CCDS30522.1.
PIRi A55731.
I52484.
RefSeqi NP_035211.2. NM_011081.2.
UniGenei Mm.3781.
Mm.401070.

3D structure databases

ProteinModelPortali Q64323.
SMRi Q64323. Positions 187-414.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT4. Glycosyltransferase Family 4.

PTM databases

PhosphoSitei Q64323.

Proteomic databases

PaxDbi Q64323.
PRIDEi Q64323.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033754 ; ENSMUSP00000033754 ; ENSMUSG00000031381 .
ENSMUST00000112255 ; ENSMUSP00000107874 ; ENSMUSG00000031381 .
GeneIDi 18700.
KEGGi mmu:18700.
UCSCi uc009uvo.1. mouse.

Organism-specific databases

CTDi 5277.
MGIi MGI:99461. Piga.

Phylogenomic databases

eggNOGi COG0438.
GeneTreei ENSGT00390000014405.
HOGENOMi HOG000203293.
HOVERGENi HBG008198.
InParanoidi Q64323.
KOi K03857.
OMAi HTGRENT.
OrthoDBi EOG7DC24J.
PhylomeDBi Q64323.
TreeFami TF105675.

Enzyme and pathway databases

UniPathwayi UPA00196 .
Reactomei REACT_243945. Synthesis of glycosylphosphatidylinositol (GPI).

Miscellaneous databases

NextBioi 294747.
PROi Q64323.
SOURCEi Search...

Gene expression databases

Bgeei Q64323.
ExpressionAtlasi Q64323. baseline and differential.
Genevestigatori Q64323.

Family and domain databases

InterProi IPR001296. Glyco_trans_1.
IPR013234. PIGA_GPI_anchor_biosynthesis.
[Graphical view ]
Pfami PF00534. Glycos_transf_1. 1 hit.
PF08288. PIGA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of murine pig-a, a gene for GPI-anchor biosynthesis, and demonstration of interspecies conservation of its structure, function, and genetic locus."
    Kawagoe K., Takeda J., Endo Y., Kinoshita T.
    Genomics 23:566-574(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129S/V.
  2. "Cloning and characterization of the mouse PIG-A gene."
    Yu J., Nagarajan S., Liu J., Young N., Medof M.E.
    Biochim. Biophys. Acta 1255:344-350(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.

Entry informationi

Entry nameiPIGA_MOUSE
AccessioniPrimary (citable) accession number: Q64323
Secondary accession number(s): Q6LD71, Q8CCQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3