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Q64323

- PIGA_MOUSE

UniProt

Q64323 - PIGA_MOUSE

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Protein
N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
Gene
Piga
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.

Pathwayi

GO - Molecular functioni

  1. transferase activity, transferring glycosyl groups Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. GPI anchor biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00196.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
Alternative name(s):
GlcNAc-PI synthesis protein
Phosphatidylinositol-glycan biosynthesis class A protein
Short name:
PIG-A
Gene namesi
Name:Piga
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:99461. Piga.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 422422Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei423 – 44321Helical; Reviewed prediction
Add
BLAST
Topological domaini444 – 48542Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: HGNC
  2. glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex Source: HGNC
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
PRO_0000080327Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei21 – 211Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64323.
PRIDEiQ64323.

PTM databases

PhosphoSiteiQ64323.

Expressioni

Gene expression databases

ArrayExpressiQ64323.
BgeeiQ64323.
GenevestigatoriQ64323.

Interactioni

Subunit structurei

Associates with PIGC, PIGH, PIGP, PIGQ and DPM2. The latter is not essential for activity. Interacts directly with PIGY By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ64323.
SMRiQ64323. Positions 206-414.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000014405.
HOGENOMiHOG000203293.
HOVERGENiHBG008198.
InParanoidiQ64323.
KOiK03857.
OMAiHTGRENT.
OrthoDBiEOG7DC24J.
PhylomeDBiQ64323.
TreeFamiTF105675.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR013234. PIGA_GPI_anchor_biosynthesis.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF08288. PIGA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64323-1 [UniParc]FASTAAdd to Basket

« Hide

MANRRGGGQG QPPSVSPSPG SSGNLSDDRT CTHNICMVSD FFYPNMGGVE    50
SHIYQLSQCL IERGHKVITV THAYGNRKGV RYLTNGLKVY YLPLRVMYNQ 100
STATTLFHSL PLLRYIFVRE RITIIHSHSS FSAMAHDALF HAKTMGLQTV 150
FTDHSLFGFA DVSSVLTNKL LTVSLCDTNH IICVSYTSKE NTVLRAALNP 200
EIVSVIPNAV DPTDFTPDPF RRHDSVITVV VVSRLVYRKG TDLLSGIIPE 250
LCQKYQELHF LIGGEGPKRI ILEEVRERYQ LHDRVQLLGA LEHKDVRNVL 300
VQGHIFLNTS LTEAFCMAIV EAASCGLQVV STKVGGIPEV LPESLIILCE 350
PSVKSLCDGL EKAIFQVKSG TLPAPENIHN VVKTFYTWRN VAERTEKVYE 400
RVSKETVLPM HKRLDRLISH CGPVTGYMFA LLAVLSYLFL IFLQWMTPDS 450
FIDVAIDATG PRRAWTHQWP RDKKRDENDK ISQSR 485
Length:485
Mass (Da):54,469
Last modified:November 1, 1996 - v1
Checksum:i12141BB48A71A03A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti107 – 1071F → I in BAC27792. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D31863 Genomic DNA. Translation: BAA06663.1.
D26047 mRNA. Translation: BAA05047.1.
S78188 mRNA. Translation: AAP32009.1.
AK032283 mRNA. Translation: BAC27792.1.
CCDSiCCDS30522.1.
PIRiA55731.
I52484.
RefSeqiNP_035211.2. NM_011081.2.
UniGeneiMm.3781.
Mm.401070.

Genome annotation databases

EnsembliENSMUST00000033754; ENSMUSP00000033754; ENSMUSG00000031381.
ENSMUST00000112255; ENSMUSP00000107874; ENSMUSG00000031381.
GeneIDi18700.
KEGGimmu:18700.
UCSCiuc009uvo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D31863 Genomic DNA. Translation: BAA06663.1 .
D26047 mRNA. Translation: BAA05047.1 .
S78188 mRNA. Translation: AAP32009.1 .
AK032283 mRNA. Translation: BAC27792.1 .
CCDSi CCDS30522.1.
PIRi A55731.
I52484.
RefSeqi NP_035211.2. NM_011081.2.
UniGenei Mm.3781.
Mm.401070.

3D structure databases

ProteinModelPortali Q64323.
SMRi Q64323. Positions 206-414.
ModBasei Search...

Protein family/group databases

CAZyi GT4. Glycosyltransferase Family 4.

PTM databases

PhosphoSitei Q64323.

Proteomic databases

PaxDbi Q64323.
PRIDEi Q64323.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033754 ; ENSMUSP00000033754 ; ENSMUSG00000031381 .
ENSMUST00000112255 ; ENSMUSP00000107874 ; ENSMUSG00000031381 .
GeneIDi 18700.
KEGGi mmu:18700.
UCSCi uc009uvo.1. mouse.

Organism-specific databases

CTDi 5277.
MGIi MGI:99461. Piga.

Phylogenomic databases

eggNOGi COG0438.
GeneTreei ENSGT00390000014405.
HOGENOMi HOG000203293.
HOVERGENi HBG008198.
InParanoidi Q64323.
KOi K03857.
OMAi HTGRENT.
OrthoDBi EOG7DC24J.
PhylomeDBi Q64323.
TreeFami TF105675.

Enzyme and pathway databases

UniPathwayi UPA00196 .

Miscellaneous databases

NextBioi 294747.
PROi Q64323.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q64323.
Bgeei Q64323.
Genevestigatori Q64323.

Family and domain databases

InterProi IPR001296. Glyco_trans_1.
IPR013234. PIGA_GPI_anchor_biosynthesis.
[Graphical view ]
Pfami PF00534. Glycos_transf_1. 1 hit.
PF08288. PIGA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of murine pig-a, a gene for GPI-anchor biosynthesis, and demonstration of interspecies conservation of its structure, function, and genetic locus."
    Kawagoe K., Takeda J., Endo Y., Kinoshita T.
    Genomics 23:566-574(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129S/V.
  2. "Cloning and characterization of the mouse PIG-A gene."
    Yu J., Nagarajan S., Liu J., Young N., Medof M.E.
    Biochim. Biophys. Acta 1255:344-350(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.

Entry informationi

Entry nameiPIGA_MOUSE
AccessioniPrimary (citable) accession number: Q64323
Secondary accession number(s): Q6LD71, Q8CCQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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