ID ZBT7B_MOUSE Reviewed; 544 AA. AC Q64321; Q80VV5; DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot. DT 16-AUG-2004, sequence version 2. DT 14-DEC-2011, entry version 94. DE RecName: Full=Zinc finger and BTB domain-containing protein 7B; DE AltName: Full=Krueppel-related zinc finger protein cKrox; DE Short=c-Krox; DE AltName: Full=T-helper-inducing POZ/Krueppel-like factor; DE AltName: Full=Zinc finger protein 67; DE Short=Zfp-67; DE AltName: Full=Zinc finger protein Th-POK; GN Name=Zbtb7b; Synonyms=Thpok, Zfp67; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=FVB/N; TISSUE=Mammary tumor; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA] OF 85-544, FUNCTION, AND TISSUE RP SPECIFICITY. RX MEDLINE=95023913; PubMed=7937772; DOI=10.1073/pnas.91.20.9372; RA Galera P., Musso M., Ducy P., Karsenty G.; RT "c-Krox, a transcriptional regulator of type I collagen gene RT expression, is preferentially expressed in skin."; RL Proc. Natl. Acad. Sci. U.S.A. 91:9372-9376(1994). RN [3] RP FUNCTION, TISSUE SPECIFICITY, AND VARIANT HD GLY-389. RX PubMed=15729333; DOI=10.1038/nature03338; RA He X., He X., Dave V.P., Zhang Y., Hua X., Nicolas E., Xu W., RA Roe B.A., Kappes D.J.; RT "The zinc finger transcription factor Th-POK regulates CD4 versus CD8 RT T-cell lineage commitment."; RL Nature 433:826-833(2005). CC -!- FUNCTION: Transcription regulator that acts as a key regulator of CC lineage commitment of immature T-cell precursors. Necessary and CC sufficient for commitment of CD4 lineage, while its absence causes CC CD8 commitment. Development of immature T-cell precursors CC (thymocytes) to either the CD4 helper or CD8 killer T-cell CC lineages correlates precisely with their T-cell receptor CC specificity for major histocompatibility complex class II or class CC I molecules, respectively. Transcriptional repressor of the CC collagen COL1A1 and COL1A2 genes. May also function as a repressor CC of fibronectin and possibly other extracellular matrix genes. CC -!- INTERACTION: CC Q8CIH5:Plcg2; NbExp=3; IntAct=EBI-642868, EBI-617954; CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- TISSUE SPECIFICITY: Widely expressed, with a higher level in skin. CC Expressed in thymus. Restricted to CD4 cells (mature single CC positive CD4(+) and intermediate CD4(+)CD8(+) cells). CC -!- DEVELOPMENTAL STAGE: Expressed, beginning at 9.5 days of gestation CC and at 10.5 days in regions destined to become skin. In adult CC animals, expression is predominantly in skin. CC -!- DISEASE: Note=Defects in Zbtb7b are the cause of helper deficient CC disease (HD). HD mice are distinguished by the virtual absence of CC peripheral T-cells of the CD4(+)CD8(-) major histocompatibility CC complex (MHC) class II-restricted T-helper subset due to a CC specific block in thymic development. The developmental defect is CC selective for CD4(+)CD8(-) cells; the maturation of CD4(-)CD8(+) CC and gamma delta T-cells is normal indicating that lineage CC commitment is specifically perturbed without affecting positive CC selection. CC -!- SIMILARITY: Contains 1 BTB (POZ) domain. CC -!- SIMILARITY: Contains 4 C2H2-type zinc fingers. CC -!- SEQUENCE CAUTION: CC Sequence=AAA61956.1; Type=Frameshift; Positions=96, 328, 336; CC Sequence=AAA61956.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA; CC Sequence=CAA85307.1; Type=Frameshift; Positions=96, 328, 336; CC Sequence=CAA85307.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA; CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; BC046533; AAH46533.1; -; mRNA. DR EMBL; L35307; AAA61956.1; ALT_SEQ; mRNA. DR EMBL; Z36549; CAA85307.1; ALT_SEQ; mRNA. DR IPI; IPI00330531; -. DR PIR; I49603; I49603. DR RefSeq; NP_033591.2; NM_009565.4. DR UniGene; Mm.262184; -. DR ProteinModelPortal; Q64321; -. DR SMR; Q64321; 7-143, 335-486. DR IntAct; Q64321; 1. DR STRING; Q64321; -. DR PhosphoSite; Q64321; -. DR PRIDE; Q64321; -. DR Ensembl; ENSMUST00000029677; ENSMUSP00000029677; ENSMUSG00000028042. DR Ensembl; ENSMUST00000107432; ENSMUSP00000103055; ENSMUSG00000028042. DR Ensembl; ENSMUST00000107433; ENSMUSP00000103056; ENSMUSG00000028042. DR Ensembl; ENSMUST00000107435; ENSMUSP00000103058; ENSMUSG00000028042. DR GeneID; 22724; -. DR KEGG; mmu:22724; -. DR CTD; 51043; -. DR MGI; MGI:102755; Zbtb7b. DR GeneTree; ENSGT00600000084004; -. DR HOGENOM; HBG716063; -. DR HOVERGEN; HBG059461; -. DR InParanoid; Q64321; -. DR OMA; DEDDCER; -. DR OrthoDB; EOG47PX6M; -. DR PhylomeDB; Q64321; -. DR NextBio; 303237; -. DR ArrayExpress; Q64321; -. DR Bgee; Q64321; -. DR CleanEx; MM_ZBTB7B; -. DR Genevestigator; Q64321; -. DR GermOnline; ENSMUSG00000028042; Mus musculus. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0003677; F:DNA binding; IDA:MGI. DR GO; GO:0042803; F:protein homodimerization activity; IDA:MGI. DR GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:MGI. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW. DR GO; GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW. DR GO; GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:MGI. DR GO; GO:0043370; P:regulation of CD4-positive, alpha-beta T cell differentiation; IMP:MGI. DR GO; GO:0043376; P:regulation of CD8-positive, alpha-beta T cell differentiation; IMP:MGI. DR GO; GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. DR InterPro; IPR000210; BTB/POZ-like. DR InterPro; IPR011333; BTB/POZ_fold. DR InterPro; IPR013069; BTB_POZ. DR InterPro; IPR007087; Znf_C2H2. DR InterPro; IPR015880; Znf_C2H2-like. DR InterPro; IPR013087; Znf_C2H2/integrase_DNA-bd. DR Gene3D; G3DSA:3.30.710.10; BTB/POZ_fold; 1. DR Gene3D; G3DSA:3.30.160.60; Znf_C2H2/integrase_DNA-bd; 4. DR KO; K10494; -. DR Pfam; PF00651; BTB; 1. DR Pfam; PF00096; zf-C2H2; 3. DR SMART; SM00225; BTB; 1. DR SMART; SM00355; ZnF_C2H2; 4. DR SUPFAM; SSF54695; BTB/POZ_fold; 1. DR PROSITE; PS50097; BTB; 1. DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3. DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4. PE 1: Evidence at protein level; KW Complete proteome; Developmental protein; Differentiation; KW Disease mutation; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; KW Reference proteome; Repeat; Repressor; Transcription; KW Transcription regulation; Zinc; Zinc-finger. FT CHAIN 1 544 Zinc finger and BTB domain-containing FT protein 7B. FT /FTId=PRO_0000047720. FT DOMAIN 34 115 BTB. FT ZN_FING 350 372 C2H2-type 1. FT ZN_FING 378 400 C2H2-type 2. FT ZN_FING 406 428 C2H2-type 3. FT ZN_FING 434 458 C2H2-type 4; atypical. FT COMPBIAS 185 200 Pro-rich. FT MOD_RES 373 373 Phosphothreonine (By similarity). FT VARIANT 389 389 R -> G (in HD). FT CONFLICT 268 268 A -> T (in Ref. 2; AAA61956/CAA85307). SQ SEQUENCE 544 AA; 58918 MW; 5A43F10AE2970709 CRC64; MGSPEDDLIG IPFPDHSSEL LSCLNEQRQL GHLCDLTIRT QGLEYRTHRA VLAACSHYFK KLFTEGGGGT VMGTGGGGTA SGGAGAGVCE LDFVGPEALG ALLEFAYTAT LTTSSANMPA VLQAARLLEI PCVIAACMEI LQGSGLEAPS PDEDDCERAR QYLEAFATAT TTASTSGMPN GEDSPPQVPL LPPPPPPPRP VARRSRKPRK AFLQTKGARA NHLVPEAPTV LTHPLTYEEE EMVGRLGNSG GSGLGDSYSP PTGAASPAEG PLNYEVFEGE EEEEEMAYPP GYGLAQSNEP SLSPEELGSD EDPIDPDLMA YLSSLHQDAL TPGLDGQDKL VRKRRSQMPQ ECPVCHKIIH GAGKLPRHMR THTGEKPFAC EVCGVRFTRN DKLKIHMRKH TGERPYSCPH CPARFLHSYD LKNHMHLHTG DRPYECHLCH KAFAKEDHLQ RHLKGQNCLE VRTRRRRKDD VAAPHYPPPS TTTSSPAGLD LSNGHLDTFH LSLARFWEQS ATTGPPVTTQ GPPEEEEEEG TPTTPQAEGA MESS //