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Q64321

- ZBT7B_MOUSE

UniProt

Q64321 - ZBT7B_MOUSE

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Protein

Zinc finger and BTB domain-containing protein 7B

Gene
Zbtb7b, Thpok, Zfp67
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri350 – 37223C2H2-type 1Add
BLAST
Zinc fingeri378 – 40023C2H2-type 2Add
BLAST
Zinc fingeri406 – 42823C2H2-type 3Add
BLAST
Zinc fingeri434 – 45825C2H2-type 4; atypicalAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: IntAct
  4. protein homodimerization activity Source: MGI
  5. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. multicellular organismal development Source: UniProtKB-KW
  3. positive regulation of gene expression Source: MGI
  4. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  5. regulation of CD4-positive, alpha-beta T cell differentiation Source: MGI
  6. regulation of CD8-positive, alpha-beta T cell differentiation Source: MGI
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger and BTB domain-containing protein 7B
Alternative name(s):
Krueppel-related zinc finger protein cKrox
Short name:
c-Krox
T-helper-inducing POZ/Krueppel-like factor
Zinc finger protein 67
Short name:
Zfp-67
Zinc finger protein Th-POK
Gene namesi
Name:Zbtb7b
Synonyms:Thpok, Zfp67
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:102755. Zbtb7b.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Zbtb7b are the cause of helper deficient disease (HD). HD mice are distinguished by the virtual absence of peripheral T-cells of the CD4+CD8- major histocompatibility complex (MHC) class II-restricted T-helper subset due to a specific block in thymic development. The developmental defect is selective for CD4+CD8- cells; the maturation of CD4-CD8+ and gamma delta T-cells is normal indicating that lineage commitment is specifically perturbed without affecting positive selection.1 Publication

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 544544Zinc finger and BTB domain-containing protein 7BPRO_0000047720Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei373 – 3731Phosphothreonine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64321.
PRIDEiQ64321.

PTM databases

PhosphoSiteiQ64321.

Expressioni

Tissue specificityi

Widely expressed, with a higher level in skin. Expressed in thymus. Restricted to CD4 cells (mature single positive CD4+ and intermediate CD4+CD8+ cells).2 Publications

Developmental stagei

Expressed, beginning at 9.5 days of gestation and at 10.5 days in regions destined to become skin. In adult animals, expression is predominantly in skin.

Gene expression databases

ArrayExpressiQ64321.
BgeeiQ64321.
CleanExiMM_ZBTB7B.
GenevestigatoriQ64321.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Plcg2Q8CIH53EBI-642868,EBI-617954
TmpoQ610292EBI-642868,EBI-6172136

Protein-protein interaction databases

BioGridi204677. 1 interaction.
IntActiQ64321. 3 interactions.
MINTiMINT-1591852.

Structurei

3D structure databases

ProteinModelPortaliQ64321.
SMRiQ64321. Positions 9-141, 319-482.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 11582BTBAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi185 – 20016Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri350 – 37223C2H2-type 1Add
BLAST
Zinc fingeri378 – 40023C2H2-type 2Add
BLAST
Zinc fingeri406 – 42823C2H2-type 3Add
BLAST
Zinc fingeri434 – 45825C2H2-type 4; atypicalAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00720000108552.
HOGENOMiHOG000082521.
HOVERGENiHBG059461.
InParanoidiQ64321.
KOiK10494.
OMAiIGIPFPE.
OrthoDBiEOG70CR6Z.
PhylomeDBiQ64321.
TreeFamiTF331824.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
3.30.710.10. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64321-1 [UniParc]FASTAAdd to Basket

« Hide

MGSPEDDLIG IPFPDHSSEL LSCLNEQRQL GHLCDLTIRT QGLEYRTHRA    50
VLAACSHYFK KLFTEGGGGT VMGTGGGGTA SGGAGAGVCE LDFVGPEALG 100
ALLEFAYTAT LTTSSANMPA VLQAARLLEI PCVIAACMEI LQGSGLEAPS 150
PDEDDCERAR QYLEAFATAT TTASTSGMPN GEDSPPQVPL LPPPPPPPRP 200
VARRSRKPRK AFLQTKGARA NHLVPEAPTV LTHPLTYEEE EMVGRLGNSG 250
GSGLGDSYSP PTGAASPAEG PLNYEVFEGE EEEEEMAYPP GYGLAQSNEP 300
SLSPEELGSD EDPIDPDLMA YLSSLHQDAL TPGLDGQDKL VRKRRSQMPQ 350
ECPVCHKIIH GAGKLPRHMR THTGEKPFAC EVCGVRFTRN DKLKIHMRKH 400
TGERPYSCPH CPARFLHSYD LKNHMHLHTG DRPYECHLCH KAFAKEDHLQ 450
RHLKGQNCLE VRTRRRRKDD VAAPHYPPPS TTTSSPAGLD LSNGHLDTFH 500
LSLARFWEQS ATTGPPVTTQ GPPEEEEEEG TPTTPQAEGA MESS 544
Length:544
Mass (Da):58,918
Last modified:August 16, 2004 - v2
Checksum:i5A43F10AE2970709
GO

Sequence cautioni

The sequence AAA61956.1 differs from that shown. Reason: Chimeric cDNA.
The sequence CAA85307.1 differs from that shown. Reason: Chimeric cDNA.
The sequence AAA61956.1 differs from that shown. Reason: Frameshift at positions 96, 328 and 336.
The sequence CAA85307.1 differs from that shown. Reason: Frameshift at positions 96, 328 and 336.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti389 – 3891R → G in HD. 1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti268 – 2681A → T in AAA61956. 1 Publication
Sequence conflicti268 – 2681A → T in CAA85307. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC046533 mRNA. Translation: AAH46533.1.
L35307 mRNA. Translation: AAA61956.1. Sequence problems.
Z36549 mRNA. Translation: CAA85307.1. Sequence problems.
CCDSiCCDS17504.1.
PIRiI49603.
RefSeqiNP_033591.2. NM_009565.4.
XP_006501376.1. XM_006501313.1.
XP_006501377.1. XM_006501314.1.
XP_006501378.1. XM_006501315.1.
XP_006501379.1. XM_006501316.1.
XP_006501380.1. XM_006501317.1.
XP_006501381.1. XM_006501318.1.
XP_006501382.1. XM_006501319.1.
UniGeneiMm.262184.

Genome annotation databases

EnsembliENSMUST00000029677; ENSMUSP00000029677; ENSMUSG00000028042.
ENSMUST00000107432; ENSMUSP00000103055; ENSMUSG00000028042.
ENSMUST00000107433; ENSMUSP00000103056; ENSMUSG00000028042.
ENSMUST00000107435; ENSMUSP00000103058; ENSMUSG00000028042.
GeneIDi22724.
KEGGimmu:22724.
UCSCiuc008pza.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC046533 mRNA. Translation: AAH46533.1 .
L35307 mRNA. Translation: AAA61956.1 . Sequence problems.
Z36549 mRNA. Translation: CAA85307.1 . Sequence problems.
CCDSi CCDS17504.1.
PIRi I49603.
RefSeqi NP_033591.2. NM_009565.4.
XP_006501376.1. XM_006501313.1.
XP_006501377.1. XM_006501314.1.
XP_006501378.1. XM_006501315.1.
XP_006501379.1. XM_006501316.1.
XP_006501380.1. XM_006501317.1.
XP_006501381.1. XM_006501318.1.
XP_006501382.1. XM_006501319.1.
UniGenei Mm.262184.

3D structure databases

ProteinModelPortali Q64321.
SMRi Q64321. Positions 9-141, 319-482.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204677. 1 interaction.
IntActi Q64321. 3 interactions.
MINTi MINT-1591852.

PTM databases

PhosphoSitei Q64321.

Proteomic databases

PaxDbi Q64321.
PRIDEi Q64321.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000029677 ; ENSMUSP00000029677 ; ENSMUSG00000028042 .
ENSMUST00000107432 ; ENSMUSP00000103055 ; ENSMUSG00000028042 .
ENSMUST00000107433 ; ENSMUSP00000103056 ; ENSMUSG00000028042 .
ENSMUST00000107435 ; ENSMUSP00000103058 ; ENSMUSG00000028042 .
GeneIDi 22724.
KEGGi mmu:22724.
UCSCi uc008pza.1. mouse.

Organism-specific databases

CTDi 51043.
MGIi MGI:102755. Zbtb7b.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00720000108552.
HOGENOMi HOG000082521.
HOVERGENi HBG059461.
InParanoidi Q64321.
KOi K10494.
OMAi IGIPFPE.
OrthoDBi EOG70CR6Z.
PhylomeDBi Q64321.
TreeFami TF331824.

Miscellaneous databases

ChiTaRSi ZBTB7B. mouse.
NextBioi 303237.
PROi Q64321.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q64321.
Bgeei Q64321.
CleanExi MM_ZBTB7B.
Genevestigatori Q64321.

Family and domain databases

Gene3Di 3.30.160.60. 4 hits.
3.30.710.10. 1 hit.
InterProi IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF00651. BTB. 1 hit.
[Graphical view ]
SMARTi SM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view ]
SUPFAMi SSF54695. SSF54695. 1 hit.
PROSITEi PS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  2. "c-Krox, a transcriptional regulator of type I collagen gene expression, is preferentially expressed in skin."
    Galera P., Musso M., Ducy P., Karsenty G.
    Proc. Natl. Acad. Sci. U.S.A. 91:9372-9376(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 85-544, FUNCTION, TISSUE SPECIFICITY.
  3. "The zinc finger transcription factor Th-POK regulates CD4 versus CD8 T-cell lineage commitment."
    He X., He X., Dave V.P., Zhang Y., Hua X., Nicolas E., Xu W., Roe B.A., Kappes D.J.
    Nature 433:826-833(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, VARIANT HD GLY-389.

Entry informationi

Entry nameiZBT7B_MOUSE
AccessioniPrimary (citable) accession number: Q64321
Secondary accession number(s): Q80VV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: July 9, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi