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Protein

Zinc finger and BTB domain-containing protein 7B

Gene

Zbtb7b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri350 – 37223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri378 – 40023C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri406 – 42823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri434 – 45825C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • multicellular organismal development Source: UniProtKB-KW
  • positive regulation of gene expression Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of CD4-positive, alpha-beta T cell differentiation Source: MGI
  • regulation of CD8-positive, alpha-beta T cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger and BTB domain-containing protein 7B
Alternative name(s):
Krueppel-related zinc finger protein cKrox
Short name:
c-Krox
T-helper-inducing POZ/Krueppel-like factor
Zinc finger protein 67
Short name:
Zfp-67
Zinc finger protein Th-POK
Gene namesi
Name:Zbtb7b
Synonyms:Thpok, Zfp67
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:102755. Zbtb7b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Zbtb7b are the cause of helper deficient disease (HD). HD mice are distinguished by the virtual absence of peripheral T-cells of the CD4(+)CD8(-) major histocompatibility complex (MHC) class II-restricted T-helper subset due to a specific block in thymic development. The developmental defect is selective for CD4(+)CD8(-) cells; the maturation of CD4(-)CD8(+) and gamma delta T-cells is normal indicating that lineage commitment is specifically perturbed without affecting positive selection.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 544544Zinc finger and BTB domain-containing protein 7BPRO_0000047720Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei150 – 1501PhosphoserineBy similarity
Modified residuei373 – 3731PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ64321.
PaxDbiQ64321.
PRIDEiQ64321.

PTM databases

PhosphoSiteiQ64321.

Expressioni

Tissue specificityi

Widely expressed, with a higher level in skin. Expressed in thymus. Restricted to CD4 cells (mature single positive CD4+ and intermediate CD4+CD8+ cells).2 Publications

Developmental stagei

Expressed, beginning at 9.5 days of gestation and at 10.5 days in regions destined to become skin. In adult animals, expression is predominantly in skin.

Gene expression databases

BgeeiQ64321.
CleanExiMM_ZBTB7B.
ExpressionAtlasiQ64321. baseline and differential.
GenevisibleiQ64321. MM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Plcg2Q8CIH53EBI-642868,EBI-617954
TmpoQ610292EBI-642868,EBI-6172136

Protein-protein interaction databases

BioGridi204677. 1 interaction.
IntActiQ64321. 3 interactions.
MINTiMINT-1591852.
STRINGi10090.ENSMUSP00000029677.

Structurei

3D structure databases

ProteinModelPortaliQ64321.
SMRiQ64321. Positions 9-141, 350-452.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 11582BTBPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi185 – 20016Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri350 – 37223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri378 – 40023C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri406 – 42823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri434 – 45825C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118922.
HOGENOMiHOG000082521.
HOVERGENiHBG059461.
InParanoidiQ64321.
KOiK10494.
OMAiIGIPFPE.
OrthoDBiEOG70CR6Z.
PhylomeDBiQ64321.
TreeFamiTF331824.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSPEDDLIG IPFPDHSSEL LSCLNEQRQL GHLCDLTIRT QGLEYRTHRA
60 70 80 90 100
VLAACSHYFK KLFTEGGGGT VMGTGGGGTA SGGAGAGVCE LDFVGPEALG
110 120 130 140 150
ALLEFAYTAT LTTSSANMPA VLQAARLLEI PCVIAACMEI LQGSGLEAPS
160 170 180 190 200
PDEDDCERAR QYLEAFATAT TTASTSGMPN GEDSPPQVPL LPPPPPPPRP
210 220 230 240 250
VARRSRKPRK AFLQTKGARA NHLVPEAPTV LTHPLTYEEE EMVGRLGNSG
260 270 280 290 300
GSGLGDSYSP PTGAASPAEG PLNYEVFEGE EEEEEMAYPP GYGLAQSNEP
310 320 330 340 350
SLSPEELGSD EDPIDPDLMA YLSSLHQDAL TPGLDGQDKL VRKRRSQMPQ
360 370 380 390 400
ECPVCHKIIH GAGKLPRHMR THTGEKPFAC EVCGVRFTRN DKLKIHMRKH
410 420 430 440 450
TGERPYSCPH CPARFLHSYD LKNHMHLHTG DRPYECHLCH KAFAKEDHLQ
460 470 480 490 500
RHLKGQNCLE VRTRRRRKDD VAAPHYPPPS TTTSSPAGLD LSNGHLDTFH
510 520 530 540
LSLARFWEQS ATTGPPVTTQ GPPEEEEEEG TPTTPQAEGA MESS
Length:544
Mass (Da):58,918
Last modified:August 16, 2004 - v2
Checksum:i5A43F10AE2970709
GO

Sequence cautioni

The sequence AAA61956.1 differs from that shown.Chimeric cDNA.Curated
The sequence AAA61956.1 differs from that shown. Reason: Frameshift at positions 96, 328 and 336. Curated
The sequence CAA85307.1 differs from that shown.Chimeric cDNA.Curated
The sequence CAA85307.1 differs from that shown. Reason: Frameshift at positions 96, 328 and 336. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti268 – 2681A → T in AAA61956 (PubMed:7937772).Curated
Sequence conflicti268 – 2681A → T in CAA85307 (PubMed:7937772).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti389 – 3891R → G in HD. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC046533 mRNA. Translation: AAH46533.1.
L35307 mRNA. Translation: AAA61956.1. Sequence problems.
Z36549 mRNA. Translation: CAA85307.1. Sequence problems.
CCDSiCCDS17504.1.
PIRiI49603.
RefSeqiNP_033591.2. NM_009565.4.
XP_006501376.1. XM_006501313.2.
XP_006501377.1. XM_006501314.2.
XP_006501378.1. XM_006501315.2.
XP_006501379.1. XM_006501316.2.
XP_006501380.1. XM_006501317.2.
XP_006501381.1. XM_006501318.2.
XP_006501382.1. XM_006501319.2.
UniGeneiMm.262184.

Genome annotation databases

EnsembliENSMUST00000029677; ENSMUSP00000029677; ENSMUSG00000028042.
ENSMUST00000107432; ENSMUSP00000103055; ENSMUSG00000028042.
ENSMUST00000107433; ENSMUSP00000103056; ENSMUSG00000028042.
ENSMUST00000107435; ENSMUSP00000103058; ENSMUSG00000028042.
GeneIDi22724.
KEGGimmu:22724.
UCSCiuc008pza.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC046533 mRNA. Translation: AAH46533.1.
L35307 mRNA. Translation: AAA61956.1. Sequence problems.
Z36549 mRNA. Translation: CAA85307.1. Sequence problems.
CCDSiCCDS17504.1.
PIRiI49603.
RefSeqiNP_033591.2. NM_009565.4.
XP_006501376.1. XM_006501313.2.
XP_006501377.1. XM_006501314.2.
XP_006501378.1. XM_006501315.2.
XP_006501379.1. XM_006501316.2.
XP_006501380.1. XM_006501317.2.
XP_006501381.1. XM_006501318.2.
XP_006501382.1. XM_006501319.2.
UniGeneiMm.262184.

3D structure databases

ProteinModelPortaliQ64321.
SMRiQ64321. Positions 9-141, 350-452.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204677. 1 interaction.
IntActiQ64321. 3 interactions.
MINTiMINT-1591852.
STRINGi10090.ENSMUSP00000029677.

PTM databases

PhosphoSiteiQ64321.

Proteomic databases

MaxQBiQ64321.
PaxDbiQ64321.
PRIDEiQ64321.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029677; ENSMUSP00000029677; ENSMUSG00000028042.
ENSMUST00000107432; ENSMUSP00000103055; ENSMUSG00000028042.
ENSMUST00000107433; ENSMUSP00000103056; ENSMUSG00000028042.
ENSMUST00000107435; ENSMUSP00000103058; ENSMUSG00000028042.
GeneIDi22724.
KEGGimmu:22724.
UCSCiuc008pza.1. mouse.

Organism-specific databases

CTDi51043.
MGIiMGI:102755. Zbtb7b.

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118922.
HOGENOMiHOG000082521.
HOVERGENiHBG059461.
InParanoidiQ64321.
KOiK10494.
OMAiIGIPFPE.
OrthoDBiEOG70CR6Z.
PhylomeDBiQ64321.
TreeFamiTF331824.

Miscellaneous databases

ChiTaRSiZbtb7b. mouse.
NextBioi303237.
PROiQ64321.
SOURCEiSearch...

Gene expression databases

BgeeiQ64321.
CleanExiMM_ZBTB7B.
ExpressionAtlasiQ64321. baseline and differential.
GenevisibleiQ64321. MM.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  2. "c-Krox, a transcriptional regulator of type I collagen gene expression, is preferentially expressed in skin."
    Galera P., Musso M., Ducy P., Karsenty G.
    Proc. Natl. Acad. Sci. U.S.A. 91:9372-9376(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 85-544, FUNCTION, TISSUE SPECIFICITY.
  3. "The zinc finger transcription factor Th-POK regulates CD4 versus CD8 T-cell lineage commitment."
    He X., He X., Dave V.P., Zhang Y., Hua X., Nicolas E., Xu W., Roe B.A., Kappes D.J.
    Nature 433:826-833(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, VARIANT HD GLY-389.

Entry informationi

Entry nameiZBT7B_MOUSE
AccessioniPrimary (citable) accession number: Q64321
Secondary accession number(s): Q80VV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: June 24, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.