Zinc finger and BTB domain-containing protein 7B

Contribute

Send feedback

Read comments (?) or add your own

Q64321 (ZBT7B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Zinc finger and BTB domain-containing protein 7B

Alternative name(s):
Krueppel-related zinc finger protein cKrox
Short name=c-Krox
T-helper-inducing POZ/Krueppel-like factor
Zinc finger protein 67
Short name=Zfp-67
Zinc finger protein Th-POK

Gene names

Name:	Zbtb7b
Synonyms:	Thpok, Zfp67

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes. Ref.2 Ref.3
Subcellular location	Nucleus.
Tissue specificity	Widely expressed, with a higher level in skin. Expressed in thymus. Restricted to CD4 cells (mature single positive CD4⁺ and intermediate CD4⁺CD8⁺ cells). Ref.2 Ref.3
Developmental stage	Expressed, beginning at 9.5 days of gestation and at 10.5 days in regions destined to become skin. In adult animals, expression is predominantly in skin.
Involvement in disease	Defects in Zbtb7b are the cause of helper deficient disease (HD). HD mice are distinguished by the virtual absence of peripheral T-cells of the CD4⁺CD8^- major histocompatibility complex (MHC) class II-restricted T-helper subset due to a specific block in thymic development. The developmental defect is selective for CD4⁺CD8^- cells; the maturation of CD4^-CD8⁺ and gamma delta T-cells is normal indicating that lineage commitment is specifically perturbed without affecting positive selection. Ref.3
Sequence similarities	Contains 1 BTB (POZ) domain. Contains 4 C2H2-type zinc fingers.
Sequence caution	The sequence AAA61956.1 differs from that shown. Reason: Frameshift at positions 96, 328 and 336. The sequence AAA61956.1 differs from that shown. Reason: Chimeric cDNA. The sequence CAA85307.1 differs from that shown. Reason: Frameshift at positions 96, 328 and 336. The sequence CAA85307.1 differs from that shown. Reason: Chimeric cDNA.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Nucleus
Disease	Disease mutation
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.2 PubMed 8702912. Source: MGI regulation of CD4-positive, alpha-beta T cell differentiation Inferred from mutant phenotype PubMed 18776904. Source: MGI regulation of CD8-positive, alpha-beta T cell differentiation Inferred from mutant phenotype PubMed 18776904. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from direct assay Ref.2 PubMed 8702912. Source: MGI protein homodimerization activity Inferred from direct assay PubMed 8702912. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 8702912. Source: MGI zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Plcg2	Q8CIH5	3	EBI-642868,EBI-617954
Tmpo	Q61029	2	EBI-642868,EBI-6172136

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 544

544

Zinc finger and BTB domain-containing protein 7B

PRO_0000047720

Regions

Domain

34 – 115

BTB

Zinc finger

350 – 372

C2H2-type 1

Zinc finger

378 – 400

C2H2-type 2

Zinc finger

406 – 428

C2H2-type 3

Zinc finger

434 – 458

C2H2-type 4; atypical

Compositional bias

185 – 200

Pro-rich

Amino acid modifications

Modified residue

373

Phosphothreonine By similarity

Natural variations

Natural variant

389

R → G in HD. Ref.3

Experimental info

Sequence conflict

268

A → T in AAA61956. Ref.2

Sequence conflict

268

A → T in CAA85307. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q64321 [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: 5A43F10AE2970709
Show »

FASTA

544

58,918

        10         20         30         40         50         60 
MGSPEDDLIG IPFPDHSSEL LSCLNEQRQL GHLCDLTIRT QGLEYRTHRA VLAACSHYFK 

        70         80         90        100        110        120 
KLFTEGGGGT VMGTGGGGTA SGGAGAGVCE LDFVGPEALG ALLEFAYTAT LTTSSANMPA 

       130        140        150        160        170        180 
VLQAARLLEI PCVIAACMEI LQGSGLEAPS PDEDDCERAR QYLEAFATAT TTASTSGMPN 

       190        200        210        220        230        240 
GEDSPPQVPL LPPPPPPPRP VARRSRKPRK AFLQTKGARA NHLVPEAPTV LTHPLTYEEE 

       250        260        270        280        290        300 
EMVGRLGNSG GSGLGDSYSP PTGAASPAEG PLNYEVFEGE EEEEEMAYPP GYGLAQSNEP 

       310        320        330        340        350        360 
SLSPEELGSD EDPIDPDLMA YLSSLHQDAL TPGLDGQDKL VRKRRSQMPQ ECPVCHKIIH 

       370        380        390        400        410        420 
GAGKLPRHMR THTGEKPFAC EVCGVRFTRN DKLKIHMRKH TGERPYSCPH CPARFLHSYD 

       430        440        450        460        470        480 
LKNHMHLHTG DRPYECHLCH KAFAKEDHLQ RHLKGQNCLE VRTRRRRKDD VAAPHYPPPS 

       490        500        510        520        530        540 
TTTSSPAGLD LSNGHLDTFH LSLARFWEQS ATTGPPVTTQ GPPEEEEEEG TPTTPQAEGA 


MESS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[2]	"c-Krox, a transcriptional regulator of type I collagen gene expression, is preferentially expressed in skin." Galera P., Musso M., Ducy P., Karsenty G. Proc. Natl. Acad. Sci. U.S.A. 91:9372-9376(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 85-544, FUNCTION, TISSUE SPECIFICITY.
[3]	"The zinc finger transcription factor Th-POK regulates CD4 versus CD8 T-cell lineage commitment." He X., He X., Dave V.P., Zhang Y., Hua X., Nicolas E., Xu W., Roe B.A., Kappes D.J. Nature 433:826-833(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, VARIANT HD GLY-389.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC046533 mRNA. Translation: AAH46533.1. L35307 mRNA. Translation: AAA61956.1. Sequence problems. Z36549 mRNA. Translation: CAA85307.1. Sequence problems.
IPI	IPI00330531.
PIR	I49603.
RefSeq	NP_033591.2. NM_009565.4.
UniGene	Mm.262184.
3D structure databases
ProteinModelPortal	Q64321.
SMR	Q64321. Positions 9-141, 348-452.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q64321. 3 interactions.
MINT	MINT-1591852.
PTM databases
PhosphoSite	Q64321.
Proteomic databases
PaxDb	Q64321.
PRIDE	Q64321.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029677; ENSMUSP00000029677; ENSMUSG00000028042. ENSMUST00000107432; ENSMUSP00000103055; ENSMUSG00000028042. ENSMUST00000107433; ENSMUSP00000103056; ENSMUSG00000028042. ENSMUST00000107435; ENSMUSP00000103058; ENSMUSG00000028042.
GeneID	22724.
KEGG	mmu:22724.
UCSC	uc008pza.1. mouse.
Organism-specific databases
CTD	51043.
MGI	MGI:102755. Zbtb7b.
Phylogenomic databases
eggNOG	COG5048.
GeneTree	ENSGT00670000097899.
HOGENOM	HOG000082521.
HOVERGEN	HBG059461.
InParanoid	Q64321.
KO	K10494.
OMA	IGIPFPE.
OrthoDB	EOG47PX6M.
Gene expression databases
ArrayExpress	Q64321.
Bgee	Q64321.
CleanEx	MM_ZBTB7B.
Genevestigator	Q64321.
GermOnline	ENSMUSG00000028042. Mus musculus.
Family and domain databases
Gene3D	3.30.160.60. 4 hits. 3.30.710.10. 1 hit.
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF00651. BTB. 1 hit. [Graphical view]
SMART	SM00225. BTB. 1 hit. SM00355. ZnF_C2H2. 4 hits. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
PROSITE	PS50097. BTB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ZBTB7B. mouse.
NextBio	303237.
SOURCE	Search...

Entry information

Entry name

ZBT7B_MOUSE

Accession

Primary (citable) accession number: Q64321
Secondary accession number(s): Q80VV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	May 29, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents