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Q64321 (ZBT7B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger and BTB domain-containing protein 7B
Alternative name(s):
Krueppel-related zinc finger protein cKrox
Short name=c-Krox
T-helper-inducing POZ/Krueppel-like factor
Zinc finger protein 67
Short name=Zfp-67
Zinc finger protein Th-POK
Gene names
Name:Zbtb7b
Synonyms:Thpok, Zfp67
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length544 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes. Ref.2 Ref.3

Subcellular location

Nucleus.

Tissue specificity

Widely expressed, with a higher level in skin. Expressed in thymus. Restricted to CD4 cells (mature single positive CD4+ and intermediate CD4+CD8+ cells). Ref.2 Ref.3

Developmental stage

Expressed, beginning at 9.5 days of gestation and at 10.5 days in regions destined to become skin. In adult animals, expression is predominantly in skin.

Involvement in disease

Defects in Zbtb7b are the cause of helper deficient disease (HD). HD mice are distinguished by the virtual absence of peripheral T-cells of the CD4+CD8- major histocompatibility complex (MHC) class II-restricted T-helper subset due to a specific block in thymic development. The developmental defect is selective for CD4+CD8- cells; the maturation of CD4-CD8+ and gamma delta T-cells is normal indicating that lineage commitment is specifically perturbed without affecting positive selection. Ref.3

Sequence similarities

Contains 1 BTB (POZ) domain.

Contains 4 C2H2-type zinc fingers.

Sequence caution

The sequence AAA61956.1 differs from that shown. Reason: Frameshift at positions 96, 328 and 336.

The sequence AAA61956.1 differs from that shown. Reason: Chimeric cDNA.

The sequence CAA85307.1 differs from that shown. Reason: Frameshift at positions 96, 328 and 336.

The sequence CAA85307.1 differs from that shown. Reason: Chimeric cDNA.

Ontologies

Keywords
   Biological processDifferentiation
Transcription
Transcription regulation
   Cellular componentNucleus
   DiseaseDisease mutation
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionDevelopmental protein
Repressor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

multicellular organismal development

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of gene expression

Inferred from direct assay PubMed 21357543. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay Ref.2PubMed 8702912. Source: MGI

regulation of CD4-positive, alpha-beta T cell differentiation

Inferred from mutant phenotype PubMed 18776904. Source: MGI

regulation of CD8-positive, alpha-beta T cell differentiation

Inferred from mutant phenotype PubMed 18776904. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from direct assay Ref.2PubMed 8702912. Source: MGI

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 15102471PubMed 22726435. Source: IntAct

protein homodimerization activity

Inferred from direct assay PubMed 8702912. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 8702912. Source: MGI

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 544544Zinc finger and BTB domain-containing protein 7B
PRO_0000047720

Regions

Domain34 – 11582BTB
Zinc finger350 – 37223C2H2-type 1
Zinc finger378 – 40023C2H2-type 2
Zinc finger406 – 42823C2H2-type 3
Zinc finger434 – 45825C2H2-type 4; atypical
Compositional bias185 – 20016Pro-rich

Amino acid modifications

Modified residue3731Phosphothreonine By similarity

Natural variations

Natural variant3891R → G in HD. Ref.3

Experimental info

Sequence conflict2681A → T in AAA61956. Ref.2
Sequence conflict2681A → T in CAA85307. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q64321 [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: 5A43F10AE2970709

FASTA54458,918
        10         20         30         40         50         60 
MGSPEDDLIG IPFPDHSSEL LSCLNEQRQL GHLCDLTIRT QGLEYRTHRA VLAACSHYFK 

        70         80         90        100        110        120 
KLFTEGGGGT VMGTGGGGTA SGGAGAGVCE LDFVGPEALG ALLEFAYTAT LTTSSANMPA 

       130        140        150        160        170        180 
VLQAARLLEI PCVIAACMEI LQGSGLEAPS PDEDDCERAR QYLEAFATAT TTASTSGMPN 

       190        200        210        220        230        240 
GEDSPPQVPL LPPPPPPPRP VARRSRKPRK AFLQTKGARA NHLVPEAPTV LTHPLTYEEE 

       250        260        270        280        290        300 
EMVGRLGNSG GSGLGDSYSP PTGAASPAEG PLNYEVFEGE EEEEEMAYPP GYGLAQSNEP 

       310        320        330        340        350        360 
SLSPEELGSD EDPIDPDLMA YLSSLHQDAL TPGLDGQDKL VRKRRSQMPQ ECPVCHKIIH 

       370        380        390        400        410        420 
GAGKLPRHMR THTGEKPFAC EVCGVRFTRN DKLKIHMRKH TGERPYSCPH CPARFLHSYD 

       430        440        450        460        470        480 
LKNHMHLHTG DRPYECHLCH KAFAKEDHLQ RHLKGQNCLE VRTRRRRKDD VAAPHYPPPS 

       490        500        510        520        530        540 
TTTSSPAGLD LSNGHLDTFH LSLARFWEQS ATTGPPVTTQ GPPEEEEEEG TPTTPQAEGA 


MESS 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[2]"c-Krox, a transcriptional regulator of type I collagen gene expression, is preferentially expressed in skin."
Galera P., Musso M., Ducy P., Karsenty G.
Proc. Natl. Acad. Sci. U.S.A. 91:9372-9376(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 85-544, FUNCTION, TISSUE SPECIFICITY.
[3]"The zinc finger transcription factor Th-POK regulates CD4 versus CD8 T-cell lineage commitment."
He X., He X., Dave V.P., Zhang Y., Hua X., Nicolas E., Xu W., Roe B.A., Kappes D.J.
Nature 433:826-833(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, VARIANT HD GLY-389.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC046533 mRNA. Translation: AAH46533.1.
L35307 mRNA. Translation: AAA61956.1. Sequence problems.
Z36549 mRNA. Translation: CAA85307.1. Sequence problems.
CCDSCCDS17504.1.
PIRI49603.
RefSeqNP_033591.2. NM_009565.4.
XP_006501376.1. XM_006501313.1.
XP_006501377.1. XM_006501314.1.
XP_006501378.1. XM_006501315.1.
XP_006501379.1. XM_006501316.1.
XP_006501380.1. XM_006501317.1.
XP_006501381.1. XM_006501318.1.
XP_006501382.1. XM_006501319.1.
UniGeneMm.262184.

3D structure databases

ProteinModelPortalQ64321.
SMRQ64321. Positions 9-141, 319-482.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204677. 1 interaction.
IntActQ64321. 3 interactions.
MINTMINT-1591852.

PTM databases

PhosphoSiteQ64321.

Proteomic databases

PaxDbQ64321.
PRIDEQ64321.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029677; ENSMUSP00000029677; ENSMUSG00000028042.
ENSMUST00000107432; ENSMUSP00000103055; ENSMUSG00000028042.
ENSMUST00000107433; ENSMUSP00000103056; ENSMUSG00000028042.
ENSMUST00000107435; ENSMUSP00000103058; ENSMUSG00000028042.
GeneID22724.
KEGGmmu:22724.
UCSCuc008pza.1. mouse.

Organism-specific databases

CTD51043.
MGIMGI:102755. Zbtb7b.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00720000108552.
HOGENOMHOG000082521.
HOVERGENHBG059461.
InParanoidQ64321.
KOK10494.
OMAIGIPFPE.
OrthoDBEOG70CR6Z.
PhylomeDBQ64321.
TreeFamTF331824.

Gene expression databases

ArrayExpressQ64321.
BgeeQ64321.
CleanExMM_ZBTB7B.
GenevestigatorQ64321.

Family and domain databases

Gene3D3.30.160.60. 4 hits.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00651. BTB. 1 hit.
[Graphical view]
SMARTSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
PROSITEPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZBTB7B. mouse.
NextBio303237.
PROQ64321.
SOURCESearch...

Entry information

Entry nameZBT7B_MOUSE
AccessionPrimary (citable) accession number: Q64321
Secondary accession number(s): Q80VV5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: July 9, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot