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Q64321

- ZBT7B_MOUSE

UniProt

Q64321 - ZBT7B_MOUSE

Protein

Zinc finger and BTB domain-containing protein 7B

Gene

Zbtb7b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri350 – 37223C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri378 – 40023C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri406 – 42823C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri434 – 45825C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. protein homodimerization activity Source: MGI
    5. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. multicellular organismal development Source: UniProtKB-KW
    3. positive regulation of gene expression Source: MGI
    4. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    5. regulation of CD4-positive, alpha-beta T cell differentiation Source: MGI
    6. regulation of CD8-positive, alpha-beta T cell differentiation Source: MGI
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Differentiation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger and BTB domain-containing protein 7B
    Alternative name(s):
    Krueppel-related zinc finger protein cKrox
    Short name:
    c-Krox
    T-helper-inducing POZ/Krueppel-like factor
    Zinc finger protein 67
    Short name:
    Zfp-67
    Zinc finger protein Th-POK
    Gene namesi
    Name:Zbtb7b
    Synonyms:Thpok, Zfp67
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:102755. Zbtb7b.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Zbtb7b are the cause of helper deficient disease (HD). HD mice are distinguished by the virtual absence of peripheral T-cells of the CD4+CD8- major histocompatibility complex (MHC) class II-restricted T-helper subset due to a specific block in thymic development. The developmental defect is selective for CD4+CD8- cells; the maturation of CD4-CD8+ and gamma delta T-cells is normal indicating that lineage commitment is specifically perturbed without affecting positive selection.

    Keywords - Diseasei

    Disease mutation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 544544Zinc finger and BTB domain-containing protein 7BPRO_0000047720Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei373 – 3731PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ64321.
    PRIDEiQ64321.

    PTM databases

    PhosphoSiteiQ64321.

    Expressioni

    Tissue specificityi

    Widely expressed, with a higher level in skin. Expressed in thymus. Restricted to CD4 cells (mature single positive CD4+ and intermediate CD4+CD8+ cells).2 Publications

    Developmental stagei

    Expressed, beginning at 9.5 days of gestation and at 10.5 days in regions destined to become skin. In adult animals, expression is predominantly in skin.

    Gene expression databases

    ArrayExpressiQ64321.
    BgeeiQ64321.
    CleanExiMM_ZBTB7B.
    GenevestigatoriQ64321.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Plcg2Q8CIH53EBI-642868,EBI-617954
    TmpoQ610292EBI-642868,EBI-6172136

    Protein-protein interaction databases

    BioGridi204677. 1 interaction.
    IntActiQ64321. 3 interactions.
    MINTiMINT-1591852.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64321.
    SMRiQ64321. Positions 9-141, 319-482.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 11582BTBPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi185 – 20016Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
    Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri350 – 37223C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri378 – 40023C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri406 – 42823C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri434 – 45825C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00720000108552.
    HOGENOMiHOG000082521.
    HOVERGENiHBG059461.
    InParanoidiQ64321.
    KOiK10494.
    OMAiIGIPFPE.
    OrthoDBiEOG70CR6Z.
    PhylomeDBiQ64321.
    TreeFamiTF331824.

    Family and domain databases

    Gene3Di3.30.160.60. 4 hits.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00651. BTB. 1 hit.
    [Graphical view]
    SMARTiSM00225. BTB. 1 hit.
    SM00355. ZnF_C2H2. 4 hits.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.
    PROSITEiPS50097. BTB. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64321-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSPEDDLIG IPFPDHSSEL LSCLNEQRQL GHLCDLTIRT QGLEYRTHRA    50
    VLAACSHYFK KLFTEGGGGT VMGTGGGGTA SGGAGAGVCE LDFVGPEALG 100
    ALLEFAYTAT LTTSSANMPA VLQAARLLEI PCVIAACMEI LQGSGLEAPS 150
    PDEDDCERAR QYLEAFATAT TTASTSGMPN GEDSPPQVPL LPPPPPPPRP 200
    VARRSRKPRK AFLQTKGARA NHLVPEAPTV LTHPLTYEEE EMVGRLGNSG 250
    GSGLGDSYSP PTGAASPAEG PLNYEVFEGE EEEEEMAYPP GYGLAQSNEP 300
    SLSPEELGSD EDPIDPDLMA YLSSLHQDAL TPGLDGQDKL VRKRRSQMPQ 350
    ECPVCHKIIH GAGKLPRHMR THTGEKPFAC EVCGVRFTRN DKLKIHMRKH 400
    TGERPYSCPH CPARFLHSYD LKNHMHLHTG DRPYECHLCH KAFAKEDHLQ 450
    RHLKGQNCLE VRTRRRRKDD VAAPHYPPPS TTTSSPAGLD LSNGHLDTFH 500
    LSLARFWEQS ATTGPPVTTQ GPPEEEEEEG TPTTPQAEGA MESS 544
    Length:544
    Mass (Da):58,918
    Last modified:August 16, 2004 - v2
    Checksum:i5A43F10AE2970709
    GO

    Sequence cautioni

    The sequence AAA61956.1 differs from that shown. Reason: Chimeric cDNA.
    The sequence CAA85307.1 differs from that shown. Reason: Chimeric cDNA.
    The sequence AAA61956.1 differs from that shown. Reason: Frameshift at positions 96, 328 and 336.
    The sequence CAA85307.1 differs from that shown. Reason: Frameshift at positions 96, 328 and 336.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti268 – 2681A → T in AAA61956. (PubMed:7937772)Curated
    Sequence conflicti268 – 2681A → T in CAA85307. (PubMed:7937772)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti389 – 3891R → G in HD. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC046533 mRNA. Translation: AAH46533.1.
    L35307 mRNA. Translation: AAA61956.1. Sequence problems.
    Z36549 mRNA. Translation: CAA85307.1. Sequence problems.
    CCDSiCCDS17504.1.
    PIRiI49603.
    RefSeqiNP_033591.2. NM_009565.4.
    XP_006501376.1. XM_006501313.1.
    XP_006501377.1. XM_006501314.1.
    XP_006501378.1. XM_006501315.1.
    XP_006501379.1. XM_006501316.1.
    XP_006501380.1. XM_006501317.1.
    XP_006501381.1. XM_006501318.1.
    XP_006501382.1. XM_006501319.1.
    UniGeneiMm.262184.

    Genome annotation databases

    EnsembliENSMUST00000029677; ENSMUSP00000029677; ENSMUSG00000028042.
    ENSMUST00000107432; ENSMUSP00000103055; ENSMUSG00000028042.
    ENSMUST00000107433; ENSMUSP00000103056; ENSMUSG00000028042.
    ENSMUST00000107435; ENSMUSP00000103058; ENSMUSG00000028042.
    GeneIDi22724.
    KEGGimmu:22724.
    UCSCiuc008pza.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC046533 mRNA. Translation: AAH46533.1 .
    L35307 mRNA. Translation: AAA61956.1 . Sequence problems.
    Z36549 mRNA. Translation: CAA85307.1 . Sequence problems.
    CCDSi CCDS17504.1.
    PIRi I49603.
    RefSeqi NP_033591.2. NM_009565.4.
    XP_006501376.1. XM_006501313.1.
    XP_006501377.1. XM_006501314.1.
    XP_006501378.1. XM_006501315.1.
    XP_006501379.1. XM_006501316.1.
    XP_006501380.1. XM_006501317.1.
    XP_006501381.1. XM_006501318.1.
    XP_006501382.1. XM_006501319.1.
    UniGenei Mm.262184.

    3D structure databases

    ProteinModelPortali Q64321.
    SMRi Q64321. Positions 9-141, 319-482.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204677. 1 interaction.
    IntActi Q64321. 3 interactions.
    MINTi MINT-1591852.

    PTM databases

    PhosphoSitei Q64321.

    Proteomic databases

    PaxDbi Q64321.
    PRIDEi Q64321.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029677 ; ENSMUSP00000029677 ; ENSMUSG00000028042 .
    ENSMUST00000107432 ; ENSMUSP00000103055 ; ENSMUSG00000028042 .
    ENSMUST00000107433 ; ENSMUSP00000103056 ; ENSMUSG00000028042 .
    ENSMUST00000107435 ; ENSMUSP00000103058 ; ENSMUSG00000028042 .
    GeneIDi 22724.
    KEGGi mmu:22724.
    UCSCi uc008pza.1. mouse.

    Organism-specific databases

    CTDi 51043.
    MGIi MGI:102755. Zbtb7b.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00720000108552.
    HOGENOMi HOG000082521.
    HOVERGENi HBG059461.
    InParanoidi Q64321.
    KOi K10494.
    OMAi IGIPFPE.
    OrthoDBi EOG70CR6Z.
    PhylomeDBi Q64321.
    TreeFami TF331824.

    Miscellaneous databases

    ChiTaRSi ZBTB7B. mouse.
    NextBioi 303237.
    PROi Q64321.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64321.
    Bgeei Q64321.
    CleanExi MM_ZBTB7B.
    Genevestigatori Q64321.

    Family and domain databases

    Gene3Di 3.30.160.60. 4 hits.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00651. BTB. 1 hit.
    [Graphical view ]
    SMARTi SM00225. BTB. 1 hit.
    SM00355. ZnF_C2H2. 4 hits.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    PROSITEi PS50097. BTB. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    2. "c-Krox, a transcriptional regulator of type I collagen gene expression, is preferentially expressed in skin."
      Galera P., Musso M., Ducy P., Karsenty G.
      Proc. Natl. Acad. Sci. U.S.A. 91:9372-9376(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 85-544, FUNCTION, TISSUE SPECIFICITY.
    3. "The zinc finger transcription factor Th-POK regulates CD4 versus CD8 T-cell lineage commitment."
      He X., He X., Dave V.P., Zhang Y., Hua X., Nicolas E., Xu W., Roe B.A., Kappes D.J.
      Nature 433:826-833(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, VARIANT HD GLY-389.

    Entry informationi

    Entry nameiZBT7B_MOUSE
    AccessioniPrimary (citable) accession number: Q64321
    Secondary accession number(s): Q80VV5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3