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Q64318 (ZEB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger E-box-binding homeobox 1
Alternative name(s):
Delta EF1
Transcription factor 8
Short name=TCF-8
Zinc finger homeobox protein 1a
Short name=MEB1
Gene names
Name:Zeb1
Synonyms:Tcf8, Zfhx1a, Zfx1a, Zfx1ha
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1117 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional repressor. Binds to E-box sequences in the immunoglobulin heavy chain enhancer as well as in the regulatory regions of many other tissue-specific genes. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1 By similarity. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Ref.5 Ref.6

Subunit structure

Interacts (via N-terminus) with SMARCA4/BRG1 By similarity.

Subcellular location

Nucleus Ref.6.

Tissue specificity

Expressed in the external germinal layer (EGL) and internal granular layer (IGL) of the cerebellum (at protein level). Ref.6

Induction

Up-regulated during NEUROD2-induced neurogenesis. Ref.6

Sequence similarities

Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Contains 7 C2H2-type zinc fingers.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processDifferentiation
Neurogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
Repeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
Repressor
   PTMIsopeptide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcartilage development

Inferred from mutant phenotype PubMed 9389660. Source: MGI

cellular response to amino acid stimulus

Inferred from direct assay PubMed 20548288. Source: MGI

cellular response to transforming growth factor beta stimulus

Inferred from electronic annotation. Source: Ensembl

central nervous system development

Inferred from genetic interaction PubMed 16598713. Source: MGI

cochlea morphogenesis

Inferred from mutant phenotype PubMed 21980308. Source: MGI

embryonic camera-type eye morphogenesis

Inferred from mutant phenotype PubMed 18436818. Source: MGI

embryonic morphogenesis

Inferred from genetic interaction PubMed 16598713. Source: MGI

embryonic skeletal system morphogenesis

Inferred from mutant phenotype PubMed 9389660. Source: MGI

forebrain development

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 18436818. Source: MGI

negative regulation of epithelial cell differentiation

Inferred from mutant phenotype PubMed 18192284. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 17392792. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from direct assay Ref.6. Source: UniProtKB

organ development

Inferred from mutant phenotype PubMed 11681996. Source: MGI

pattern specification process

Inferred from mutant phenotype PubMed 9389660. Source: MGI

positive regulation of neuron differentiation

Inferred from direct assay Ref.6. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16824956. Source: MGI

regulation of T cell differentiation in thymus

Inferred from mutant phenotype PubMed 9126927PubMed 9389660. Source: MGI

regulation of mesenchymal cell proliferation

Inferred from mutant phenotype PubMed 18192284. Source: MGI

regulation of smooth muscle cell differentiation

Inferred from mutant phenotype PubMed 16824956. Source: MGI

regulation of transforming growth factor beta receptor signaling pathway

Inferred from genetic interaction PubMed 16824956. Source: MGI

response to activity

Inferred from electronic annotation. Source: Ensembl

response to nutrient levels

Inferred from electronic annotation. Source: Ensembl

semicircular canal morphogenesis

Inferred from mutant phenotype PubMed 21980308. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay Ref.6. Source: UniProtKB

transcription factor complex

Inferred from direct assay PubMed 17392792. Source: MGI

   Molecular_functionE-box binding

Inferred from direct assay Ref.6. Source: UniProtKB

chromatin binding

Inferred from direct assay PubMed 16824956. Source: MGI

double-stranded DNA binding

Inferred from electronic annotation. Source: Ensembl

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 21224849. Source: IntAct

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 17392792. Source: MGI

transcription factor binding

Inferred from physical interaction PubMed 17392792. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Ctbp1O887124EBI-8560245,EBI-604547

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11171117Zinc finger E-box-binding homeobox 1
PRO_0000047233

Regions

Zinc finger150 – 17324C2H2-type 1
Zinc finger180 – 20223C2H2-type 2
Zinc finger220 – 24223C2H2-type 3
Zinc finger248 – 27225C2H2-type 4; atypical
DNA binding559 – 61860Homeobox; atypical Ref.6
Zinc finger882 – 90423C2H2-type 5
Zinc finger910 – 93223C2H2-type 6
Zinc finger938 – 95922C2H2-type 7; atypical
Compositional bias969 – 1117149Glu-rich (acidic)

Amino acid modifications

Modified residue3021Phosphoserine By similarity
Modified residue6571Phosphoserine By similarity
Modified residue6801Phosphothreonine By similarity
Cross-link327Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link752Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Experimental info

Sequence conflict331S → A in AAB08442. Ref.3
Sequence conflict571M → K in AAB08442. Ref.3
Sequence conflict1251Missing in AAB08442. Ref.3
Sequence conflict1461F → L in AAB08442. Ref.3
Sequence conflict3131E → EV in AAB08442. Ref.3
Sequence conflict3531G → A in AAB08442. Ref.3
Sequence conflict4001V → L in AAB08442. Ref.3
Sequence conflict4611Q → E in AAB08442. Ref.3
Sequence conflict5281K → KK in AAB08442. Ref.3
Sequence conflict6641S → T in AAB08442. Ref.3
Sequence conflict7441E → L in AAB08442. Ref.3
Sequence conflict8221T → A in AAB08442. Ref.3
Sequence conflict10621Missing in AAB08442. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q64318 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D1FAC2D048D34237

FASTA1,117122,465
        10         20         30         40         50         60 
MADGPRCKRR KQANPRRNNV TNYNTVVEAN SDSDDEDKLH IVEEESITDA ADCEGGMPDD 

        70         80         90        100        110        120 
ELPADQTVLP GGSDRGGGAK NCWQDNVKDN ECDSDAENEQ NHDPNVEEFL QQQDTAVIYP 

       130        140        150        160        170        180 
EAPEEDQRQG TPEASSHDEN GTPDAFSQLL TCPYCDRGYK RFTSLKEHIK YRHEKNEDNF 

       190        200        210        220        230        240 
SCSLCSYTFA YRTQLERHMT SHKSGREQRH VTQSGGNRKF KCTECGKAFK YKHHLKEHLR 

       250        260        270        280        290        300 
IHSGEKPYEC PNCKKRFSHS GSYSSHISSK KCISLMPVNG RPRSGLKTSQ CSSPSLSTSP 

       310        320        330        340        350        360 
GSPTRPQIRQ KIENKPLQEP LSVNQIKTEP VDYEFKPIVV ASGINCSTPL QNGVFSSGGQ 

       370        380        390        400        410        420 
LQATSSPQGV VQAVVLPTVG LVSPISINLS DIQNVLKVAV DGNVIRQVLE TNQASLASKE 

       430        440        450        460        470        480 
QEAVSASPIQ QGGHSVISAI SLPLVDQDGT TKIIINYSLE QPSQLQVVPQ NLKKEIPAPT 

       490        500        510        520        530        540 
NSCKSEKLPE DLTVKSETDK SFEGARDDST CLLCEDCPGD LNALPELKHY DPECPAQPPP 

       550        560        570        580        590        600 
PAPATEKPES SASSAGNGDL SPSQPPLKNL LSLLKAYYAL NAQPSTEELS KIADSVNLPL 

       610        620        630        640        650        660 
DGVKKWFEKM QAGQIPGQSP DPPSPGTGSV NIPTKTDEQP QPADGNEPQE DSTRGQSPVK 

       670        680        690        700        710        720 
IRSSPVLPVG SAMNGSRSCT SSPSPLNLCS ARNPQGYSCV AEGAQEEPQV EPLDLSLPKQ 

       730        740        750        760        770        780 
QGELLERSTV SSVYQNSVYS VQEEPLNLSC AKKEPQKDSC VTDSEPVVNV VPPSANPINI 

       790        800        810        820        830        840 
AIPTVTAQLP TIVAIADQNS VPCLRALAAN KQTILIPQVA YTYSATVSPA VQEPPVKVIQ 

       850        860        870        880        890        900 
PNGNQDERQD TSSEGVSTVE DQNDSDSTPP KKKTRKTENG MYACDLCDKI FQKSSSLLRH 

       910        920        930        940        950        960 
KYEHTGKRPH ECGICRKAFK HKHHLIEHMR LHSGEKPYQC DKCGKRFSHS GSYSQHMNHR 

       970        980        990       1000       1010       1020 
YSYCKRGAED RDAMEQEDAG PEVLPEVLAT EHVGARASPS QADSDERESL TREEDEDSEK 

      1030       1040       1050       1060       1070       1080 
EEEEEDKEME ELQEGKECEN PQGEEEEEEE EEEEEEEEEE EEVEADEAEH EAAAKTDGTV 

      1090       1100       1110 
EVGAAQQAGS LEQKASESEM ESESESEQLS EEKTNEA 

« Hide

References

« Hide 'large scale' references
[1]"Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains."
Sekido R., Takagi T., Okanami M., Moribe H., Yamamura M., Higashi Y., Kondoh H.
Gene 173:227-232(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Embryo.
[2]Wu Y., Montoya G.D., Rubin S.E., Brodie S.G., Jenkins N., Williams T.M.
Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Spleen.
[3]"Cloning of a cDNA encoding a mouse transcriptional repressor displaying striking sequence conservation across vertebrates."
Genetta T., Kadesch T.
Gene 169:289-290(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Brain.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The EMT-activator ZEB1 promotes tumorigenicity by repressing stemness-inhibiting microRNAs."
Wellner U., Schubert J., Burk U.C., Schmalhofer O., Zhu F., Sonntag A., Waldvogel B., Vannier C., Darling D., zur Hausen A., Brunton V.G., Morton J., Sansom O., Schuler J., Stemmler M.P., Herzberger C., Hopt U., Keck T., Brabletz S., Brabletz T.
Nat. Cell Biol. 11:1487-1495(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Transcriptional inhibition of REST by NeuroD2 during neuronal differentiation."
Ravanpay A.C., Hansen S.J., Olson J.M.
Mol. Cell. Neurosci. 44:178-189(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U26259 mRNA. Translation: AAA67564.1.
D76432 mRNA. Translation: BAA11177.1.
L48363 Genomic DNA. Translation: AAB08442.1.
BC139769 mRNA. Translation: AAI39770.1.
CCDSCCDS29039.1.
PIRJC4934.
RefSeqNP_035676.1. NM_011546.3.
UniGeneMm.3929.

3D structure databases

ProteinModelPortalQ64318.
SMRQ64318. Positions 152-320, 561-619, 881-981.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204010. 2 interactions.
DIPDIP-60280N.
IntActQ64318. 1 interaction.

PTM databases

PhosphoSiteQ64318.

Proteomic databases

PRIDEQ64318.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025081; ENSMUSP00000025081; ENSMUSG00000024238.
GeneID21417.
KEGGmmu:21417.
UCSCuc008dyy.2. mouse.

Organism-specific databases

CTD6935.
MGIMGI:1344313. Zeb1.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00630000089829.
HOGENOMHOG000264256.
HOVERGENHBG004697.
InParanoidA4QPD2.
KOK09299.
OMACPGDINA.
OrthoDBEOG790G0D.
PhylomeDBQ64318.
TreeFamTF331759.

Gene expression databases

ArrayExpressQ64318.
BgeeQ64318.
CleanExMM_ZEB1.
GenevestigatorQ64318.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
3.30.160.60. 5 hits.
InterProIPR008598. Di19_Zn_binding.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF05605. zf-Di19. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZEB1. mouse.
NextBio300720.
PROQ64318.
SOURCESearch...

Entry information

Entry nameZEB1_MOUSE
AccessionPrimary (citable) accession number: Q64318
Secondary accession number(s): A4QPD2, Q62519
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot