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Q64318

- ZEB1_MOUSE

UniProt

Q64318 - ZEB1_MOUSE

Protein

Zinc finger E-box-binding homeobox 1

Gene

Zeb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Acts as a transcriptional repressor. Binds to E-box sequences in the immunoglobulin heavy chain enhancer as well as in the regulatory regions of many other tissue-specific genes. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1 By similarity. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.By similarity2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri150 – 17324C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri180 – 20223C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri220 – 24223C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri248 – 27225C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi559 – 61860Homeobox; atypicalAdd
    BLAST
    Zinc fingeri882 – 90423C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri910 – 93223C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri938 – 95922C2H2-type 7; atypicalPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. double-stranded DNA binding Source: Ensembl
    3. E-box binding Source: UniProtKB
    4. metal ion binding Source: UniProtKB-KW
    5. protein binding Source: IntAct
    6. sequence-specific DNA binding Source: InterPro
    7. sequence-specific DNA binding transcription factor activity Source: MGI
    8. transcription factor binding Source: MGI

    GO - Biological processi

    1. cartilage development Source: MGI
    2. cellular response to amino acid stimulus Source: MGI
    3. cellular response to transforming growth factor beta stimulus Source: Ensembl
    4. central nervous system development Source: MGI
    5. cochlea morphogenesis Source: MGI
    6. embryonic camera-type eye morphogenesis Source: MGI
    7. embryonic morphogenesis Source: MGI
    8. embryonic skeletal system morphogenesis Source: MGI
    9. forebrain development Source: Ensembl
    10. negative regulation of cell proliferation Source: MGI
    11. negative regulation of epithelial cell differentiation Source: MGI
    12. negative regulation of transcription, DNA-templated Source: UniProtKB
    13. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    14. organ development Source: MGI
    15. pattern specification process Source: MGI
    16. positive regulation of neuron differentiation Source: UniProtKB
    17. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    18. regulation of mesenchymal cell proliferation Source: MGI
    19. regulation of smooth muscle cell differentiation Source: MGI
    20. regulation of T cell differentiation in thymus Source: MGI
    21. regulation of transforming growth factor beta receptor signaling pathway Source: MGI
    22. response to activity Source: Ensembl
    23. response to nutrient levels Source: Ensembl
    24. semicircular canal morphogenesis Source: MGI
    25. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Differentiation, Neurogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger E-box-binding homeobox 1
    Alternative name(s):
    Delta EF1
    Transcription factor 8
    Short name:
    TCF-8
    Zinc finger homeobox protein 1a
    Short name:
    MEB1
    Gene namesi
    Name:Zeb1
    Synonyms:Tcf8, Zfhx1a, Zfx1a, Zfx1ha
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1344313. Zeb1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleus Source: UniProtKB
    3. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11171117Zinc finger E-box-binding homeobox 1PRO_0000047233Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei302 – 3021PhosphoserineBy similarity
    Cross-linki327 – 327Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei657 – 6571PhosphoserineBy similarity
    Modified residuei680 – 6801PhosphothreonineBy similarity
    Cross-linki752 – 752Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ64318.

    PTM databases

    PhosphoSiteiQ64318.

    Expressioni

    Tissue specificityi

    Expressed in the external germinal layer (EGL) and internal granular layer (IGL) of the cerebellum (at protein level).1 Publication

    Inductioni

    Up-regulated during NEUROD2-induced neurogenesis.1 Publication

    Gene expression databases

    ArrayExpressiQ64318.
    BgeeiQ64318.
    CleanExiMM_ZEB1.
    GenevestigatoriQ64318.

    Interactioni

    Subunit structurei

    Interacts (via N-terminus) with SMARCA4/BRG1.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Ctbp1O887124EBI-8560245,EBI-604547

    Protein-protein interaction databases

    BioGridi204010. 2 interactions.
    DIPiDIP-60280N.
    IntActiQ64318. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64318.
    SMRiQ64318. Positions 152-320, 561-619, 881-981.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi969 – 1117149Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 homeobox DNA-binding domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri150 – 17324C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri180 – 20223C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri220 – 24223C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri248 – 27225C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri882 – 90423C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri910 – 93223C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri938 – 95922C2H2-type 7; atypicalPROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Homeobox, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00630000089829.
    HOGENOMiHOG000264256.
    HOVERGENiHBG004697.
    InParanoidiA4QPD2.
    KOiK09299.
    OMAiCPGDINA.
    OrthoDBiEOG790G0D.
    PhylomeDBiQ64318.
    TreeFamiTF331759.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    3.30.160.60. 5 hits.
    InterProiIPR008598. Di19_Zn_binding.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF05605. zf-Di19. 1 hit.
    [Graphical view]
    SMARTiSM00389. HOX. 1 hit.
    SM00355. ZnF_C2H2. 7 hits.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
    PS50157. ZINC_FINGER_C2H2_2. 6 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64318-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADGPRCKRR KQANPRRNNV TNYNTVVEAN SDSDDEDKLH IVEEESITDA     50
    ADCEGGMPDD ELPADQTVLP GGSDRGGGAK NCWQDNVKDN ECDSDAENEQ 100
    NHDPNVEEFL QQQDTAVIYP EAPEEDQRQG TPEASSHDEN GTPDAFSQLL 150
    TCPYCDRGYK RFTSLKEHIK YRHEKNEDNF SCSLCSYTFA YRTQLERHMT 200
    SHKSGREQRH VTQSGGNRKF KCTECGKAFK YKHHLKEHLR IHSGEKPYEC 250
    PNCKKRFSHS GSYSSHISSK KCISLMPVNG RPRSGLKTSQ CSSPSLSTSP 300
    GSPTRPQIRQ KIENKPLQEP LSVNQIKTEP VDYEFKPIVV ASGINCSTPL 350
    QNGVFSSGGQ LQATSSPQGV VQAVVLPTVG LVSPISINLS DIQNVLKVAV 400
    DGNVIRQVLE TNQASLASKE QEAVSASPIQ QGGHSVISAI SLPLVDQDGT 450
    TKIIINYSLE QPSQLQVVPQ NLKKEIPAPT NSCKSEKLPE DLTVKSETDK 500
    SFEGARDDST CLLCEDCPGD LNALPELKHY DPECPAQPPP PAPATEKPES 550
    SASSAGNGDL SPSQPPLKNL LSLLKAYYAL NAQPSTEELS KIADSVNLPL 600
    DGVKKWFEKM QAGQIPGQSP DPPSPGTGSV NIPTKTDEQP QPADGNEPQE 650
    DSTRGQSPVK IRSSPVLPVG SAMNGSRSCT SSPSPLNLCS ARNPQGYSCV 700
    AEGAQEEPQV EPLDLSLPKQ QGELLERSTV SSVYQNSVYS VQEEPLNLSC 750
    AKKEPQKDSC VTDSEPVVNV VPPSANPINI AIPTVTAQLP TIVAIADQNS 800
    VPCLRALAAN KQTILIPQVA YTYSATVSPA VQEPPVKVIQ PNGNQDERQD 850
    TSSEGVSTVE DQNDSDSTPP KKKTRKTENG MYACDLCDKI FQKSSSLLRH 900
    KYEHTGKRPH ECGICRKAFK HKHHLIEHMR LHSGEKPYQC DKCGKRFSHS 950
    GSYSQHMNHR YSYCKRGAED RDAMEQEDAG PEVLPEVLAT EHVGARASPS 1000
    QADSDERESL TREEDEDSEK EEEEEDKEME ELQEGKECEN PQGEEEEEEE 1050
    EEEEEEEEEE EEVEADEAEH EAAAKTDGTV EVGAAQQAGS LEQKASESEM 1100
    ESESESEQLS EEKTNEA 1117
    Length:1,117
    Mass (Da):122,465
    Last modified:November 1, 1996 - v1
    Checksum:iD1FAC2D048D34237
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti33 – 331S → A in AAB08442. (PubMed:8647466)Curated
    Sequence conflicti57 – 571M → K in AAB08442. (PubMed:8647466)Curated
    Sequence conflicti125 – 1251Missing in AAB08442. (PubMed:8647466)Curated
    Sequence conflicti146 – 1461F → L in AAB08442. (PubMed:8647466)Curated
    Sequence conflicti313 – 3131E → EV in AAB08442. (PubMed:8647466)Curated
    Sequence conflicti353 – 3531G → A in AAB08442. (PubMed:8647466)Curated
    Sequence conflicti400 – 4001V → L in AAB08442. (PubMed:8647466)Curated
    Sequence conflicti461 – 4611Q → E in AAB08442. (PubMed:8647466)Curated
    Sequence conflicti528 – 5281K → KK in AAB08442. (PubMed:8647466)Curated
    Sequence conflicti664 – 6641S → T in AAB08442. (PubMed:8647466)Curated
    Sequence conflicti744 – 7441E → L in AAB08442. (PubMed:8647466)Curated
    Sequence conflicti822 – 8221T → A in AAB08442. (PubMed:8647466)Curated
    Sequence conflicti1062 – 10621Missing in AAB08442. (PubMed:8647466)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U26259 mRNA. Translation: AAA67564.1.
    D76432 mRNA. Translation: BAA11177.1.
    L48363 Genomic DNA. Translation: AAB08442.1.
    BC139769 mRNA. Translation: AAI39770.1.
    CCDSiCCDS29039.1.
    PIRiJC4934.
    RefSeqiNP_035676.1. NM_011546.3.
    UniGeneiMm.3929.

    Genome annotation databases

    EnsembliENSMUST00000025081; ENSMUSP00000025081; ENSMUSG00000024238.
    GeneIDi21417.
    KEGGimmu:21417.
    UCSCiuc008dyy.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U26259 mRNA. Translation: AAA67564.1 .
    D76432 mRNA. Translation: BAA11177.1 .
    L48363 Genomic DNA. Translation: AAB08442.1 .
    BC139769 mRNA. Translation: AAI39770.1 .
    CCDSi CCDS29039.1.
    PIRi JC4934.
    RefSeqi NP_035676.1. NM_011546.3.
    UniGenei Mm.3929.

    3D structure databases

    ProteinModelPortali Q64318.
    SMRi Q64318. Positions 152-320, 561-619, 881-981.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204010. 2 interactions.
    DIPi DIP-60280N.
    IntActi Q64318. 1 interaction.

    PTM databases

    PhosphoSitei Q64318.

    Proteomic databases

    PRIDEi Q64318.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025081 ; ENSMUSP00000025081 ; ENSMUSG00000024238 .
    GeneIDi 21417.
    KEGGi mmu:21417.
    UCSCi uc008dyy.2. mouse.

    Organism-specific databases

    CTDi 6935.
    MGIi MGI:1344313. Zeb1.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00630000089829.
    HOGENOMi HOG000264256.
    HOVERGENi HBG004697.
    InParanoidi A4QPD2.
    KOi K09299.
    OMAi CPGDINA.
    OrthoDBi EOG790G0D.
    PhylomeDBi Q64318.
    TreeFami TF331759.

    Miscellaneous databases

    ChiTaRSi ZEB1. mouse.
    NextBioi 300720.
    PROi Q64318.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64318.
    Bgeei Q64318.
    CleanExi MM_ZEB1.
    Genevestigatori Q64318.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    3.30.160.60. 5 hits.
    InterProi IPR008598. Di19_Zn_binding.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF05605. zf-Di19. 1 hit.
    [Graphical view ]
    SMARTi SM00389. HOX. 1 hit.
    SM00355. ZnF_C2H2. 7 hits.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 5 hits.
    PS50157. ZINC_FINGER_C2H2_2. 6 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains."
      Sekido R., Takagi T., Okanami M., Moribe H., Yamamura M., Higashi Y., Kondoh H.
      Gene 173:227-232(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Embryo.
    2. Wu Y., Montoya G.D., Rubin S.E., Brodie S.G., Jenkins N., Williams T.M.
      Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: Spleen.
    3. "Cloning of a cDNA encoding a mouse transcriptional repressor displaying striking sequence conservation across vertebrates."
      Genetta T., Kadesch T.
      Gene 169:289-290(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Brain.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. Cited for: FUNCTION.
    6. "Transcriptional inhibition of REST by NeuroD2 during neuronal differentiation."
      Ravanpay A.C., Hansen S.J., Olson J.M.
      Mol. Cell. Neurosci. 44:178-189(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiZEB1_MOUSE
    AccessioniPrimary (citable) accession number: Q64318
    Secondary accession number(s): A4QPD2, Q62519
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3