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Q64318

- ZEB1_MOUSE

UniProt

Q64318 - ZEB1_MOUSE

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Protein
Zinc finger E-box-binding homeobox 1
Gene
Zeb1, Tcf8, Zfhx1a, Zfx1a, Zfx1ha
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Binds to E-box sequences in the immunoglobulin heavy chain enhancer as well as in the regulatory regions of many other tissue-specific genes. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1 By similarity. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri150 – 17324C2H2-type 1
Add
BLAST
Zinc fingeri180 – 20223C2H2-type 2
Add
BLAST
Zinc fingeri220 – 24223C2H2-type 3
Add
BLAST
Zinc fingeri248 – 27225C2H2-type 4; atypical
Add
BLAST
DNA bindingi559 – 61860Homeobox; atypical1 Publication
Add
BLAST
Zinc fingeri882 – 90423C2H2-type 5
Add
BLAST
Zinc fingeri910 – 93223C2H2-type 6
Add
BLAST
Zinc fingeri938 – 95922C2H2-type 7; atypical
Add
BLAST

GO - Molecular functioni

  1. E-box binding Source: UniProtKB
  2. chromatin binding Source: MGI
  3. double-stranded DNA binding Source: Ensembl
  4. metal ion binding Source: UniProtKB-KW
  5. protein binding Source: IntAct
  6. sequence-specific DNA binding Source: InterPro
  7. sequence-specific DNA binding transcription factor activity Source: MGI
  8. transcription factor binding Source: MGI

GO - Biological processi

  1. cartilage development Source: MGI
  2. cellular response to amino acid stimulus Source: MGI
  3. cellular response to transforming growth factor beta stimulus Source: Ensembl
  4. central nervous system development Source: MGI
  5. cochlea morphogenesis Source: MGI
  6. embryonic camera-type eye morphogenesis Source: MGI
  7. embryonic morphogenesis Source: MGI
  8. embryonic skeletal system morphogenesis Source: MGI
  9. forebrain development Source: Ensembl
  10. negative regulation of cell proliferation Source: MGI
  11. negative regulation of epithelial cell differentiation Source: MGI
  12. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  13. negative regulation of transcription, DNA-templated Source: UniProtKB
  14. organ development Source: MGI
  15. pattern specification process Source: MGI
  16. positive regulation of neuron differentiation Source: UniProtKB
  17. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  18. regulation of T cell differentiation in thymus Source: MGI
  19. regulation of mesenchymal cell proliferation Source: MGI
  20. regulation of smooth muscle cell differentiation Source: MGI
  21. regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  22. response to activity Source: Ensembl
  23. response to nutrient levels Source: Ensembl
  24. semicircular canal morphogenesis Source: MGI
  25. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger E-box-binding homeobox 1
Alternative name(s):
Delta EF1
Transcription factor 8
Short name:
TCF-8
Zinc finger homeobox protein 1a
Short name:
MEB1
Gene namesi
Name:Zeb1
Synonyms:Tcf8, Zfhx1a, Zfx1a, Zfx1ha
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:1344313. Zeb1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: UniProtKB
  3. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11171117Zinc finger E-box-binding homeobox 1
PRO_0000047233Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei302 – 3021Phosphoserine By similarity
Cross-linki327 – 327Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Modified residuei657 – 6571Phosphoserine By similarity
Modified residuei680 – 6801Phosphothreonine By similarity
Cross-linki752 – 752Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ64318.

PTM databases

PhosphoSiteiQ64318.

Expressioni

Tissue specificityi

Expressed in the external germinal layer (EGL) and internal granular layer (IGL) of the cerebellum (at protein level).1 Publication

Inductioni

Up-regulated during NEUROD2-induced neurogenesis.1 Publication

Gene expression databases

ArrayExpressiQ64318.
BgeeiQ64318.
CleanExiMM_ZEB1.
GenevestigatoriQ64318.

Interactioni

Subunit structurei

Interacts (via N-terminus) with SMARCA4/BRG1 By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Ctbp1O887124EBI-8560245,EBI-604547

Protein-protein interaction databases

BioGridi204010. 2 interactions.
DIPiDIP-60280N.
IntActiQ64318. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ64318.
SMRiQ64318. Positions 152-320, 561-619, 881-981.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi969 – 1117149Glu-rich (acidic)
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00630000089829.
HOGENOMiHOG000264256.
HOVERGENiHBG004697.
InParanoidiA4QPD2.
KOiK09299.
OMAiCPGDINA.
OrthoDBiEOG790G0D.
PhylomeDBiQ64318.
TreeFamiTF331759.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.160.60. 5 hits.
InterProiIPR008598. Di19_Zn_binding.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF05605. zf-Di19. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64318-1 [UniParc]FASTAAdd to Basket

« Hide

MADGPRCKRR KQANPRRNNV TNYNTVVEAN SDSDDEDKLH IVEEESITDA     50
ADCEGGMPDD ELPADQTVLP GGSDRGGGAK NCWQDNVKDN ECDSDAENEQ 100
NHDPNVEEFL QQQDTAVIYP EAPEEDQRQG TPEASSHDEN GTPDAFSQLL 150
TCPYCDRGYK RFTSLKEHIK YRHEKNEDNF SCSLCSYTFA YRTQLERHMT 200
SHKSGREQRH VTQSGGNRKF KCTECGKAFK YKHHLKEHLR IHSGEKPYEC 250
PNCKKRFSHS GSYSSHISSK KCISLMPVNG RPRSGLKTSQ CSSPSLSTSP 300
GSPTRPQIRQ KIENKPLQEP LSVNQIKTEP VDYEFKPIVV ASGINCSTPL 350
QNGVFSSGGQ LQATSSPQGV VQAVVLPTVG LVSPISINLS DIQNVLKVAV 400
DGNVIRQVLE TNQASLASKE QEAVSASPIQ QGGHSVISAI SLPLVDQDGT 450
TKIIINYSLE QPSQLQVVPQ NLKKEIPAPT NSCKSEKLPE DLTVKSETDK 500
SFEGARDDST CLLCEDCPGD LNALPELKHY DPECPAQPPP PAPATEKPES 550
SASSAGNGDL SPSQPPLKNL LSLLKAYYAL NAQPSTEELS KIADSVNLPL 600
DGVKKWFEKM QAGQIPGQSP DPPSPGTGSV NIPTKTDEQP QPADGNEPQE 650
DSTRGQSPVK IRSSPVLPVG SAMNGSRSCT SSPSPLNLCS ARNPQGYSCV 700
AEGAQEEPQV EPLDLSLPKQ QGELLERSTV SSVYQNSVYS VQEEPLNLSC 750
AKKEPQKDSC VTDSEPVVNV VPPSANPINI AIPTVTAQLP TIVAIADQNS 800
VPCLRALAAN KQTILIPQVA YTYSATVSPA VQEPPVKVIQ PNGNQDERQD 850
TSSEGVSTVE DQNDSDSTPP KKKTRKTENG MYACDLCDKI FQKSSSLLRH 900
KYEHTGKRPH ECGICRKAFK HKHHLIEHMR LHSGEKPYQC DKCGKRFSHS 950
GSYSQHMNHR YSYCKRGAED RDAMEQEDAG PEVLPEVLAT EHVGARASPS 1000
QADSDERESL TREEDEDSEK EEEEEDKEME ELQEGKECEN PQGEEEEEEE 1050
EEEEEEEEEE EEVEADEAEH EAAAKTDGTV EVGAAQQAGS LEQKASESEM 1100
ESESESEQLS EEKTNEA 1117
Length:1,117
Mass (Da):122,465
Last modified:November 1, 1996 - v1
Checksum:iD1FAC2D048D34237
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331S → A in AAB08442. 1 Publication
Sequence conflicti57 – 571M → K in AAB08442. 1 Publication
Sequence conflicti125 – 1251Missing in AAB08442. 1 Publication
Sequence conflicti146 – 1461F → L in AAB08442. 1 Publication
Sequence conflicti313 – 3131E → EV in AAB08442. 1 Publication
Sequence conflicti353 – 3531G → A in AAB08442. 1 Publication
Sequence conflicti400 – 4001V → L in AAB08442. 1 Publication
Sequence conflicti461 – 4611Q → E in AAB08442. 1 Publication
Sequence conflicti528 – 5281K → KK in AAB08442. 1 Publication
Sequence conflicti664 – 6641S → T in AAB08442. 1 Publication
Sequence conflicti744 – 7441E → L in AAB08442. 1 Publication
Sequence conflicti822 – 8221T → A in AAB08442. 1 Publication
Sequence conflicti1062 – 10621Missing in AAB08442. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U26259 mRNA. Translation: AAA67564.1.
D76432 mRNA. Translation: BAA11177.1.
L48363 Genomic DNA. Translation: AAB08442.1.
BC139769 mRNA. Translation: AAI39770.1.
CCDSiCCDS29039.1.
PIRiJC4934.
RefSeqiNP_035676.1. NM_011546.3.
UniGeneiMm.3929.

Genome annotation databases

EnsembliENSMUST00000025081; ENSMUSP00000025081; ENSMUSG00000024238.
GeneIDi21417.
KEGGimmu:21417.
UCSCiuc008dyy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U26259 mRNA. Translation: AAA67564.1 .
D76432 mRNA. Translation: BAA11177.1 .
L48363 Genomic DNA. Translation: AAB08442.1 .
BC139769 mRNA. Translation: AAI39770.1 .
CCDSi CCDS29039.1.
PIRi JC4934.
RefSeqi NP_035676.1. NM_011546.3.
UniGenei Mm.3929.

3D structure databases

ProteinModelPortali Q64318.
SMRi Q64318. Positions 152-320, 561-619, 881-981.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204010. 2 interactions.
DIPi DIP-60280N.
IntActi Q64318. 1 interaction.

PTM databases

PhosphoSitei Q64318.

Proteomic databases

PRIDEi Q64318.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025081 ; ENSMUSP00000025081 ; ENSMUSG00000024238 .
GeneIDi 21417.
KEGGi mmu:21417.
UCSCi uc008dyy.2. mouse.

Organism-specific databases

CTDi 6935.
MGIi MGI:1344313. Zeb1.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00630000089829.
HOGENOMi HOG000264256.
HOVERGENi HBG004697.
InParanoidi A4QPD2.
KOi K09299.
OMAi CPGDINA.
OrthoDBi EOG790G0D.
PhylomeDBi Q64318.
TreeFami TF331759.

Miscellaneous databases

ChiTaRSi ZEB1. mouse.
NextBioi 300720.
PROi Q64318.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q64318.
Bgeei Q64318.
CleanExi MM_ZEB1.
Genevestigatori Q64318.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
3.30.160.60. 5 hits.
InterProi IPR008598. Di19_Zn_binding.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF05605. zf-Di19. 1 hit.
[Graphical view ]
SMARTi SM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains."
    Sekido R., Takagi T., Okanami M., Moribe H., Yamamura M., Higashi Y., Kondoh H.
    Gene 173:227-232(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Embryo.
  2. Wu Y., Montoya G.D., Rubin S.E., Brodie S.G., Jenkins N., Williams T.M.
    Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Spleen.
  3. "Cloning of a cDNA encoding a mouse transcriptional repressor displaying striking sequence conservation across vertebrates."
    Genetta T., Kadesch T.
    Gene 169:289-290(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: FUNCTION.
  6. "Transcriptional inhibition of REST by NeuroD2 during neuronal differentiation."
    Ravanpay A.C., Hansen S.J., Olson J.M.
    Mol. Cell. Neurosci. 44:178-189(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiZEB1_MOUSE
AccessioniPrimary (citable) accession number: Q64318
Secondary accession number(s): A4QPD2, Q62519
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi