Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q64318

- ZEB1_MOUSE

UniProt

Q64318 - ZEB1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Zinc finger E-box-binding homeobox 1

Gene

Zeb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Binds to E-box sequences in the immunoglobulin heavy chain enhancer as well as in the regulatory regions of many other tissue-specific genes. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1 (By similarity). Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri150 – 17324C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri180 – 20223C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri220 – 24223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 27225C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST
DNA bindingi559 – 61860Homeobox; atypicalAdd
BLAST
Zinc fingeri882 – 90423C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri910 – 93223C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri938 – 95922C2H2-type 7; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. double-stranded DNA binding Source: Ensembl
  3. E-box binding Source: UniProtKB
  4. metal ion binding Source: UniProtKB-KW
  5. sequence-specific DNA binding transcription factor activity Source: MGI
  6. transcription factor binding Source: MGI

GO - Biological processi

  1. cartilage development Source: MGI
  2. cellular response to amino acid stimulus Source: MGI
  3. cellular response to transforming growth factor beta stimulus Source: Ensembl
  4. central nervous system development Source: MGI
  5. cochlea morphogenesis Source: MGI
  6. embryonic camera-type eye morphogenesis Source: MGI
  7. embryonic morphogenesis Source: MGI
  8. embryonic skeletal system morphogenesis Source: MGI
  9. forebrain development Source: Ensembl
  10. negative regulation of cell proliferation Source: MGI
  11. negative regulation of epithelial cell differentiation Source: MGI
  12. negative regulation of transcription, DNA-templated Source: UniProtKB
  13. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  14. organ development Source: MGI
  15. pattern specification process Source: MGI
  16. positive regulation of neuron differentiation Source: UniProtKB
  17. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  18. regulation of mesenchymal cell proliferation Source: MGI
  19. regulation of smooth muscle cell differentiation Source: MGI
  20. regulation of T cell differentiation in thymus Source: MGI
  21. regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  22. response to activity Source: Ensembl
  23. response to nutrient levels Source: Ensembl
  24. semicircular canal morphogenesis Source: MGI
  25. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger E-box-binding homeobox 1
Alternative name(s):
Delta EF1
Transcription factor 8
Short name:
TCF-8
Zinc finger homeobox protein 1a
Short name:
MEB1
Gene namesi
Name:Zeb1
Synonyms:Tcf8, Zfhx1a, Zfx1a, Zfx1ha
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:1344313. Zeb1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: UniProtKB
  3. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11171117Zinc finger E-box-binding homeobox 1PRO_0000047233Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei302 – 3021PhosphoserineBy similarity
Cross-linki327 – 327Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei657 – 6571PhosphoserineBy similarity
Modified residuei680 – 6801PhosphothreonineBy similarity
Cross-linki752 – 752Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ64318.
PRIDEiQ64318.

PTM databases

PhosphoSiteiQ64318.

Expressioni

Tissue specificityi

Expressed in the external germinal layer (EGL) and internal granular layer (IGL) of the cerebellum (at protein level).1 Publication

Inductioni

Up-regulated during NEUROD2-induced neurogenesis.1 Publication

Gene expression databases

BgeeiQ64318.
CleanExiMM_ZEB1.
ExpressionAtlasiQ64318. baseline and differential.
GenevestigatoriQ64318.

Interactioni

Subunit structurei

Interacts (via N-terminus) with SMARCA4/BRG1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Ctbp1O887124EBI-8560245,EBI-604547

Protein-protein interaction databases

BioGridi204010. 2 interactions.
DIPiDIP-60280N.
IntActiQ64318. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ64318.
SMRiQ64318. Positions 152-320, 561-619, 881-981.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi969 – 1117149Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 homeobox DNA-binding domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri150 – 17324C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri180 – 20223C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri220 – 24223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 27225C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri882 – 90423C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri910 – 93223C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri938 – 95922C2H2-type 7; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00630000089829.
HOGENOMiHOG000264256.
HOVERGENiHBG004697.
InParanoidiQ64318.
KOiK09299.
OMAiCPGDINA.
OrthoDBiEOG790G0D.
PhylomeDBiQ64318.
TreeFamiTF331759.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.160.60. 5 hits.
InterProiIPR008598. Di19_Zn_binding.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF05605. zf-Di19. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64318-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADGPRCKRR KQANPRRNNV TNYNTVVEAN SDSDDEDKLH IVEEESITDA
60 70 80 90 100
ADCEGGMPDD ELPADQTVLP GGSDRGGGAK NCWQDNVKDN ECDSDAENEQ
110 120 130 140 150
NHDPNVEEFL QQQDTAVIYP EAPEEDQRQG TPEASSHDEN GTPDAFSQLL
160 170 180 190 200
TCPYCDRGYK RFTSLKEHIK YRHEKNEDNF SCSLCSYTFA YRTQLERHMT
210 220 230 240 250
SHKSGREQRH VTQSGGNRKF KCTECGKAFK YKHHLKEHLR IHSGEKPYEC
260 270 280 290 300
PNCKKRFSHS GSYSSHISSK KCISLMPVNG RPRSGLKTSQ CSSPSLSTSP
310 320 330 340 350
GSPTRPQIRQ KIENKPLQEP LSVNQIKTEP VDYEFKPIVV ASGINCSTPL
360 370 380 390 400
QNGVFSSGGQ LQATSSPQGV VQAVVLPTVG LVSPISINLS DIQNVLKVAV
410 420 430 440 450
DGNVIRQVLE TNQASLASKE QEAVSASPIQ QGGHSVISAI SLPLVDQDGT
460 470 480 490 500
TKIIINYSLE QPSQLQVVPQ NLKKEIPAPT NSCKSEKLPE DLTVKSETDK
510 520 530 540 550
SFEGARDDST CLLCEDCPGD LNALPELKHY DPECPAQPPP PAPATEKPES
560 570 580 590 600
SASSAGNGDL SPSQPPLKNL LSLLKAYYAL NAQPSTEELS KIADSVNLPL
610 620 630 640 650
DGVKKWFEKM QAGQIPGQSP DPPSPGTGSV NIPTKTDEQP QPADGNEPQE
660 670 680 690 700
DSTRGQSPVK IRSSPVLPVG SAMNGSRSCT SSPSPLNLCS ARNPQGYSCV
710 720 730 740 750
AEGAQEEPQV EPLDLSLPKQ QGELLERSTV SSVYQNSVYS VQEEPLNLSC
760 770 780 790 800
AKKEPQKDSC VTDSEPVVNV VPPSANPINI AIPTVTAQLP TIVAIADQNS
810 820 830 840 850
VPCLRALAAN KQTILIPQVA YTYSATVSPA VQEPPVKVIQ PNGNQDERQD
860 870 880 890 900
TSSEGVSTVE DQNDSDSTPP KKKTRKTENG MYACDLCDKI FQKSSSLLRH
910 920 930 940 950
KYEHTGKRPH ECGICRKAFK HKHHLIEHMR LHSGEKPYQC DKCGKRFSHS
960 970 980 990 1000
GSYSQHMNHR YSYCKRGAED RDAMEQEDAG PEVLPEVLAT EHVGARASPS
1010 1020 1030 1040 1050
QADSDERESL TREEDEDSEK EEEEEDKEME ELQEGKECEN PQGEEEEEEE
1060 1070 1080 1090 1100
EEEEEEEEEE EEVEADEAEH EAAAKTDGTV EVGAAQQAGS LEQKASESEM
1110
ESESESEQLS EEKTNEA
Length:1,117
Mass (Da):122,465
Last modified:November 1, 1996 - v1
Checksum:iD1FAC2D048D34237
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331S → A in AAB08442. (PubMed:8647466)Curated
Sequence conflicti57 – 571M → K in AAB08442. (PubMed:8647466)Curated
Sequence conflicti125 – 1251Missing in AAB08442. (PubMed:8647466)Curated
Sequence conflicti146 – 1461F → L in AAB08442. (PubMed:8647466)Curated
Sequence conflicti313 – 3131E → EV in AAB08442. (PubMed:8647466)Curated
Sequence conflicti353 – 3531G → A in AAB08442. (PubMed:8647466)Curated
Sequence conflicti400 – 4001V → L in AAB08442. (PubMed:8647466)Curated
Sequence conflicti461 – 4611Q → E in AAB08442. (PubMed:8647466)Curated
Sequence conflicti528 – 5281K → KK in AAB08442. (PubMed:8647466)Curated
Sequence conflicti664 – 6641S → T in AAB08442. (PubMed:8647466)Curated
Sequence conflicti744 – 7441E → L in AAB08442. (PubMed:8647466)Curated
Sequence conflicti822 – 8221T → A in AAB08442. (PubMed:8647466)Curated
Sequence conflicti1062 – 10621Missing in AAB08442. (PubMed:8647466)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26259 mRNA. Translation: AAA67564.1.
D76432 mRNA. Translation: BAA11177.1.
L48363 Genomic DNA. Translation: AAB08442.1.
BC139769 mRNA. Translation: AAI39770.1.
CCDSiCCDS29039.1.
PIRiJC4934.
RefSeqiNP_035676.1. NM_011546.3.
UniGeneiMm.3929.

Genome annotation databases

EnsembliENSMUST00000025081; ENSMUSP00000025081; ENSMUSG00000024238.
GeneIDi21417.
KEGGimmu:21417.
UCSCiuc008dyy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26259 mRNA. Translation: AAA67564.1 .
D76432 mRNA. Translation: BAA11177.1 .
L48363 Genomic DNA. Translation: AAB08442.1 .
BC139769 mRNA. Translation: AAI39770.1 .
CCDSi CCDS29039.1.
PIRi JC4934.
RefSeqi NP_035676.1. NM_011546.3.
UniGenei Mm.3929.

3D structure databases

ProteinModelPortali Q64318.
SMRi Q64318. Positions 152-320, 561-619, 881-981.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204010. 2 interactions.
DIPi DIP-60280N.
IntActi Q64318. 1 interaction.

PTM databases

PhosphoSitei Q64318.

Proteomic databases

MaxQBi Q64318.
PRIDEi Q64318.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025081 ; ENSMUSP00000025081 ; ENSMUSG00000024238 .
GeneIDi 21417.
KEGGi mmu:21417.
UCSCi uc008dyy.2. mouse.

Organism-specific databases

CTDi 6935.
MGIi MGI:1344313. Zeb1.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00630000089829.
HOGENOMi HOG000264256.
HOVERGENi HBG004697.
InParanoidi Q64318.
KOi K09299.
OMAi CPGDINA.
OrthoDBi EOG790G0D.
PhylomeDBi Q64318.
TreeFami TF331759.

Miscellaneous databases

ChiTaRSi Zeb1. mouse.
NextBioi 300720.
PROi Q64318.
SOURCEi Search...

Gene expression databases

Bgeei Q64318.
CleanExi MM_ZEB1.
ExpressionAtlasi Q64318. baseline and differential.
Genevestigatori Q64318.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
3.30.160.60. 5 hits.
InterProi IPR008598. Di19_Zn_binding.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF05605. zf-Di19. 1 hit.
[Graphical view ]
SMARTi SM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains."
    Sekido R., Takagi T., Okanami M., Moribe H., Yamamura M., Higashi Y., Kondoh H.
    Gene 173:227-232(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Embryo.
  2. Wu Y., Montoya G.D., Rubin S.E., Brodie S.G., Jenkins N., Williams T.M.
    Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Spleen.
  3. "Cloning of a cDNA encoding a mouse transcriptional repressor displaying striking sequence conservation across vertebrates."
    Genetta T., Kadesch T.
    Gene 169:289-290(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: FUNCTION.
  6. "Transcriptional inhibition of REST by NeuroD2 during neuronal differentiation."
    Ravanpay A.C., Hansen S.J., Olson J.M.
    Mol. Cell. Neurosci. 44:178-189(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiZEB1_MOUSE
AccessioniPrimary (citable) accession number: Q64318
Secondary accession number(s): A4QPD2, Q62519
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3