Q64314 (CD34_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 110.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Hematopoietic progenitor cell antigen CD34 Alternative name(s): CD_antigen=CD34 | ||
| Gene names |
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| Organism | Mus musculus (Mouse) [Reference proteome] | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 382 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins By similarity. |
| Subcellular location | |
| Tissue specificity | Highly expressed in hematopoietic progenitor cell lines, brain and testis, and moderately in the thymus, spleen, and bone marrow, but not in adult liver. |
| Post-translational modification | Highly glycosylated By similarity. Phosphorylated on serine residues by PKC By similarity. |
| Sequence similarities | Belongs to the CD34 family. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Long (identifier: Q64314-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Short (identifier: Q64314-2) The sequence of this isoform differs from the canonical sequence as follows: 322-325: GEDP → ELEP 326-382: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 34 | 34 | Potential | ||||||
| Chain | 35 – 382 | 348 | Hematopoietic progenitor cell antigen CD34 | PRO_0000020901 | |||||
Regions | |||||||||
| Topological domain | 35 – 287 | 253 | Extracellular Potential | ||||||
| Transmembrane | 288 – 308 | 21 | Helical; Potential | ||||||
| Topological domain | 309 – 382 | 74 | Cytoplasmic Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 326 | 1 | Phosphotyrosine Ref.4 | ||||||
| Modified residue | 336 | 1 | Phosphotyrosine Ref.4 | ||||||
| Glycosylation | 50 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 56 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 87 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 137 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 144 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 145 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 246 | 1 | N-linked (GlcNAc...) Ref.5 | ||||||
Natural variations | |||||||||
| Alternative sequence | 322 – 325 | 4 | GEDP → ELEP in isoform Short. | VSP_004161 | |||||
| Alternative sequence | 326 – 382 | 57 | Missing in isoform Short. | VSP_004162 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The gene encoding the stem cell antigen, CD34, is conserved in mouse and expressed in haemopoietic progenitor cell lines, brain, and embryonic fibroblasts." Brown J., Greaves M.F., Molgaard H.V. Int. Immunol. 3:175-184(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG). Strain: 129 and BALB/c. Tissue: Bone marrow. |
| [2] | "Two types of murine CD34 mRNA generated by alternative splicing." Suda J., Sudo T., Ito M., Ohno N., Yamaguchi Y., Suda T. Blood 79:2288-2295(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS LONG AND SHORT). |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Strain: FVB/N. Tissue: Mammary gland. |
| [4] | "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-326 AND TYR-336, MASS SPECTROMETRY. Tissue: Mast cell. |
| [5] | "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation." Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B. Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-246, MASS SPECTROMETRY. Tissue: Myoblast. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | S69293 mRNA. Translation: AAB19246.1. S69302 Genomic DNA. No translation available. S69295 Genomic DNA. No translation available. S69299 mRNA. Translation: AAB22108.1. Sequence problems. S69301 mRNA. Translation: AAB22109.1. BC006607 mRNA. Translation: AAH06607.1. |
| IPI | IPI00133257. IPI00222348. |
| PIR | I65354. |
| RefSeq | NP_598415.1. NM_133654.3. |
| UniGene | Mm.29798. |
3D structure databases | |
| ProteinModelPortal | Q64314. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q64314. |
Proteomic databases | |
| PaxDb | Q64314. |
| PRIDE | Q64314. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000016638; ENSMUSP00000016638; ENSMUSG00000016494. ENSMUST00000110815; ENSMUSP00000106439; ENSMUSG00000016494. |
| GeneID | 12490. |
| KEGG | mmu:12490. |
Organism-specific databases | |
| CTD | 947. |
| MGI | MGI:88329. Cd34. |
Phylogenomic databases | |
| eggNOG | NOG42285. |
| GeneTree | ENSGT00390000008414. |
| HOGENOM | HOG000052513. |
| HOVERGEN | HBG003461. |
| InParanoid | Q64314. |
| KO | K06474. |
| OMA | GMPRGWT. |
| OrthoDB | EOG4GF3G0. |
Gene expression databases | |
| ArrayExpress | Q64314. |
| Bgee | Q64314. |
| CleanEx | MM_CD34. |
| Genevestigator | Q64314. |
| GermOnline | ENSMUSG00000016494. Mus musculus. |
Family and domain databases | |
| InterPro | IPR008083. CD34. IPR013836. CD34/Podocalyxin. [Graphical view] |
| PANTHER | PTHR16677. PTHR16677. 1 hit. |
| Pfam | PF06365. CD34_antigen. 1 hit. [Graphical view] |
| PRINTS | PR01700. CD34ANTIGEN. |
| ProtoNet | Search... |
Other | |
| NextBio | 281408. |
| SOURCE | Search... |
Entry information
| Entry name | CD34_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q64314 Secondary accession number(s): Q62550, Q62551 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |