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Q64314

- CD34_MOUSE

UniProt

Q64314 - CD34_MOUSE

Protein

Hematopoietic progenitor cell antigen CD34

Gene

Cd34

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins By similarity.By similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: UniProtKB
    2. sulfate binding Source: MGI

    GO - Biological processi

    1. cell proliferation Source: UniProtKB
    2. endothelial cell proliferation Source: Ensembl
    3. endothelium development Source: Ensembl
    4. extracellular vesicular exosome assembly Source: Ensembl
    5. glomerular endothelium development Source: Ensembl
    6. glomerular filtration Source: UniProtKB
    7. glutamate metabolic process Source: UniProtKB
    8. hematopoietic stem cell proliferation Source: Ensembl
    9. leukocyte migration Source: MGI
    10. mesangial cell-matrix adhesion Source: UniProtKB
    11. metanephric glomerular mesangial cell differentiation Source: Ensembl
    12. negative regulation of blood coagulation Source: UniProtKB
    13. negative regulation of cellular response to heat Source: UniProtKB
    14. negative regulation of cellular response to hypoxia Source: UniProtKB
    15. negative regulation of gene expression Source: Ensembl
    16. negative regulation of interleukin-2 secretion Source: Ensembl
    17. negative regulation of necrotic cell death Source: Ensembl
    18. negative regulation of neuron death Source: UniProtKB
    19. negative regulation of nitric oxide biosynthetic process Source: UniProtKB
    20. negative regulation of tumor necrosis factor production Source: UniProtKB
    21. paracrine signaling Source: Ensembl
    22. positive regulation of angiogenesis Source: UniProtKB
    23. positive regulation of gene expression Source: Ensembl
    24. positive regulation of glial cell line-derived neurotrophic factor secretion Source: Ensembl
    25. positive regulation of granulocyte colony-stimulating factor production Source: Ensembl
    26. positive regulation of interleukin-10 production Source: UniProtKB
    27. positive regulation of odontogenesis Source: UniProtKB
    28. positive regulation of transforming growth factor beta production Source: UniProtKB
    29. positive regulation of vasculogenesis Source: UniProtKB
    30. regulation of blood pressure Source: UniProtKB
    31. signal transduction Source: Ensembl
    32. single organismal cell-cell adhesion Source: UniProtKB
    33. tissue homeostasis Source: UniProtKB
    34. transdifferentiation Source: Ensembl
    35. vascular wound healing Source: Ensembl

    Keywords - Biological processi

    Cell adhesion

    Enzyme and pathway databases

    ReactomeiREACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hematopoietic progenitor cell antigen CD34
    Alternative name(s):
    CD_antigen: CD34
    Gene namesi
    Name:Cd34
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:88329. Cd34.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB
    2. basal plasma membrane Source: UniProtKB
    3. cell periphery Source: MGI
    4. cell surface Source: UniProtKB
    5. cytoplasm Source: MGI
    6. external side of plasma membrane Source: MGI
    7. extracellular region Source: MGI
    8. glomerular endothelium fenestra Source: UniProtKB
    9. intercellular bridge Source: UniProtKB
    10. lysosome Source: UniProtKB
    11. membrane Source: MGI
    12. perinuclear region of cytoplasm Source: UniProtKB
    13. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3434Sequence AnalysisAdd
    BLAST
    Chaini35 – 382348Hematopoietic progenitor cell antigen CD34PRO_0000020901Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi87 – 871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi144 – 1441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi246 – 2461N-linked (GlcNAc...)1 Publication
    Modified residuei326 – 3261Phosphotyrosine1 Publication
    Modified residuei336 – 3361Phosphotyrosine1 Publication

    Post-translational modificationi

    Highly glycosylated.By similarity
    Phosphorylated on serine residues by PKC.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ64314.
    PaxDbiQ64314.
    PRIDEiQ64314.

    PTM databases

    PhosphoSiteiQ64314.

    Expressioni

    Tissue specificityi

    Highly expressed in hematopoietic progenitor cell lines, brain and testis, and moderately in the thymus, spleen, and bone marrow, but not in adult liver.

    Gene expression databases

    ArrayExpressiQ64314.
    BgeeiQ64314.
    CleanExiMM_CD34.
    GenevestigatoriQ64314.

    Interactioni

    Protein-protein interaction databases

    IntActiQ64314. 1 interaction.
    MINTiMINT-4090207.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64314.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini35 – 287253ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini309 – 38274CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei288 – 30821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CD34 family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG42285.
    GeneTreeiENSGT00390000008414.
    HOGENOMiHOG000052513.
    HOVERGENiHBG003461.
    InParanoidiQ64314.
    KOiK06474.
    OMAiKGEIKCS.
    OrthoDBiEOG7CCBSQ.
    PhylomeDBiQ64314.
    TreeFamiTF335795.

    Family and domain databases

    InterProiIPR008083. CD34.
    IPR013836. CD34/Podocalyxin.
    [Graphical view]
    PANTHERiPTHR16677. PTHR16677. 1 hit.
    PfamiPF06365. CD34_antigen. 1 hit.
    [Graphical view]
    PRINTSiPR01700. CD34ANTIGEN.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: Q64314-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQVHRDTRAG LLLPWRWVAL CLMSLLHLNN LTSATTETST QGISPSVPTN    50
    ESVEENITSS IPGSTSHYLI YQDSSKTTPA ISETMVNFTV TSGIPSGSGT 100
    PHTFSQPQTS PTGILPTTSD SISTSEMTWK SSLPSINVSD YSPNNSSFEM 150
    TSPTEPYAYT SSSAPSAIKG EIKCSGIREV RLAQGICLEL SEASSCEEFK 200
    KEKGEDLIQI LCEKEEAEAD AGASVCSLLL AQSEVRPECL LMVLANSTEL 250
    PSKLQLMEKH QSDLRKLGIQ SFNKQDIGSH QSYSRKTLIA LVTSGVLLAI 300
    LGTTGYFLMN RRSWSPTGER LGEDPYYTEN GGGQGYSSGP GASPETQGKA 350
    NVTRGAQENG TGQATSRNGH SARQHVVADT EL 382
    Length:382
    Mass (Da):40,983
    Last modified:November 1, 1996 - v1
    Checksum:iEAE611ABE85C4957
    GO
    Isoform Short (identifier: Q64314-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         322-325: GEDP → ELEP
         326-382: Missing.

    Show »
    Length:325
    Mass (Da):35,263
    Checksum:i940BDA6660E65C4C
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei322 – 3254GEDP → ELEP in isoform Short. CuratedVSP_004161
    Alternative sequencei326 – 38257Missing in isoform Short. CuratedVSP_004162Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S69293 mRNA. Translation: AAB19246.1.
    S69302 Genomic DNA. No translation available.
    S69295 Genomic DNA. No translation available.
    S69299 mRNA. Translation: AAB22108.1. Sequence problems.
    S69301 mRNA. Translation: AAB22109.1.
    BC006607 mRNA. Translation: AAH06607.1.
    CCDSiCCDS15640.1. [Q64314-1]
    CCDS48490.1. [Q64314-2]
    PIRiI65354.
    RefSeqiNP_598415.1. NM_133654.3. [Q64314-1]
    UniGeneiMm.29798.

    Genome annotation databases

    EnsembliENSMUST00000016638; ENSMUSP00000016638; ENSMUSG00000016494. [Q64314-1]
    ENSMUST00000110815; ENSMUSP00000106439; ENSMUSG00000016494. [Q64314-2]
    GeneIDi12490.
    KEGGimmu:12490.
    UCSCiuc007eeq.2. mouse. [Q64314-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S69293 mRNA. Translation: AAB19246.1 .
    S69302 Genomic DNA. No translation available.
    S69295 Genomic DNA. No translation available.
    S69299 mRNA. Translation: AAB22108.1 . Sequence problems.
    S69301 mRNA. Translation: AAB22109.1 .
    BC006607 mRNA. Translation: AAH06607.1 .
    CCDSi CCDS15640.1. [Q64314-1 ]
    CCDS48490.1. [Q64314-2 ]
    PIRi I65354.
    RefSeqi NP_598415.1. NM_133654.3. [Q64314-1 ]
    UniGenei Mm.29798.

    3D structure databases

    ProteinModelPortali Q64314.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q64314. 1 interaction.
    MINTi MINT-4090207.

    PTM databases

    PhosphoSitei Q64314.

    Proteomic databases

    MaxQBi Q64314.
    PaxDbi Q64314.
    PRIDEi Q64314.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000016638 ; ENSMUSP00000016638 ; ENSMUSG00000016494 . [Q64314-1 ]
    ENSMUST00000110815 ; ENSMUSP00000106439 ; ENSMUSG00000016494 . [Q64314-2 ]
    GeneIDi 12490.
    KEGGi mmu:12490.
    UCSCi uc007eeq.2. mouse. [Q64314-1 ]

    Organism-specific databases

    CTDi 947.
    MGIi MGI:88329. Cd34.

    Phylogenomic databases

    eggNOGi NOG42285.
    GeneTreei ENSGT00390000008414.
    HOGENOMi HOG000052513.
    HOVERGENi HBG003461.
    InParanoidi Q64314.
    KOi K06474.
    OMAi KGEIKCS.
    OrthoDBi EOG7CCBSQ.
    PhylomeDBi Q64314.
    TreeFami TF335795.

    Enzyme and pathway databases

    Reactomei REACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

    Miscellaneous databases

    NextBioi 281408.
    PROi Q64314.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64314.
    Bgeei Q64314.
    CleanExi MM_CD34.
    Genevestigatori Q64314.

    Family and domain databases

    InterProi IPR008083. CD34.
    IPR013836. CD34/Podocalyxin.
    [Graphical view ]
    PANTHERi PTHR16677. PTHR16677. 1 hit.
    Pfami PF06365. CD34_antigen. 1 hit.
    [Graphical view ]
    PRINTSi PR01700. CD34ANTIGEN.
    ProtoNeti Search...

    Publicationsi

    1. "The gene encoding the stem cell antigen, CD34, is conserved in mouse and expressed in haemopoietic progenitor cell lines, brain, and embryonic fibroblasts."
      Brown J., Greaves M.F., Molgaard H.V.
      Int. Immunol. 3:175-184(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG).
      Strain: 129 and BALB/c.
      Tissue: Bone marrow.
    2. "Two types of murine CD34 mRNA generated by alternative splicing."
      Suda J., Sudo T., Ito M., Ohno N., Yamaguchi Y., Suda T.
      Blood 79:2288-2295(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS LONG AND SHORT).
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Strain: FVB/N.
      Tissue: Mammary gland.
    4. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
      Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
      J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-326 AND TYR-336, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Mast cell.
    6. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
      Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
      Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-246.
      Tissue: Myoblast.

    Entry informationi

    Entry nameiCD34_MOUSE
    AccessioniPrimary (citable) accession number: Q64314
    Secondary accession number(s): Q62550, Q62551
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3