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Q64314 (CD34_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hematopoietic progenitor cell antigen CD34
Alternative name(s):
CD_antigen=CD34
Gene names
Name:Cd34
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Highly expressed in hematopoietic progenitor cell lines, brain and testis, and moderately in the thymus, spleen, and bone marrow, but not in adult liver.

Post-translational modification

Highly glycosylated By similarity.

Phosphorylated on serine residues by PKC By similarity.

Sequence similarities

Belongs to the CD34 family.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

cell-cell adhesion

Inferred from sequence or structural similarity. Source: UniProtKB

endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

endothelium development

Inferred from electronic annotation. Source: Ensembl

extracellular vesicular exosome assembly

Inferred from electronic annotation. Source: Ensembl

glomerular endothelium development

Inferred from electronic annotation. Source: Ensembl

glomerular filtration

Inferred from sequence or structural similarity. Source: UniProtKB

glutamate metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

hematopoietic stem cell proliferation

Inferred from electronic annotation. Source: Ensembl

leukocyte migration

Inferred from mutant phenotype PubMed 15701716. Source: MGI

mesangial cell-matrix adhesion

Inferred from sequence or structural similarity. Source: UniProtKB

metanephric glomerular mesangial cell differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of blood coagulation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cellular response to heat

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cellular response to hypoxia

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of gene expression

Inferred from electronic annotation. Source: Ensembl

negative regulation of interleukin-2 secretion

Inferred from electronic annotation. Source: Ensembl

negative regulation of necrotic cell death

Inferred from electronic annotation. Source: Ensembl

negative regulation of neuron death

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of nitric oxide biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of tumor necrosis factor production

Inferred from sequence or structural similarity. Source: UniProtKB

paracrine signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of angiogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of gene expression

Inferred from electronic annotation. Source: Ensembl

positive regulation of glial cell line-derived neurotrophic factor secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of granulocyte colony-stimulating factor production

Inferred from electronic annotation. Source: Ensembl

positive regulation of interleukin-10 production

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of odontogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transforming growth factor beta production

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of vasculogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of blood pressure

Inferred from sequence or structural similarity. Source: UniProtKB

signal transduction

Inferred from electronic annotation. Source: Ensembl

tissue homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

transdifferentiation

Inferred from electronic annotation. Source: Ensembl

vascular wound healing

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentapical plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

basal plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

cell periphery

Inferred from direct assay PubMed 21464233. Source: MGI

cell surface

Inferred from direct assay PubMed 19833765. Source: UniProtKB

cytoplasm

Inferred from direct assay PubMed 17717145. Source: MGI

external side of plasma membrane

Inferred from direct assay PubMed 10943842PubMed 12714519PubMed 12900455PubMed 17882221PubMed 21708977. Source: MGI

extracellular region

Inferred from direct assay PubMed 14597732. Source: MGI

glomerular endothelium fenestra

Inferred from sequence or structural similarity. Source: UniProtKB

intercellular bridge

Inferred from sequence or structural similarity. Source: UniProtKB

lysosome

Inferred from sequence or structural similarity. Source: UniProtKB

membrane

Inferred from direct assay PubMed 17717145. Source: MGI

perinuclear region of cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functioncarbohydrate binding

Inferred from sequence or structural similarity. Source: UniProtKB

sulfate binding

Inferred from direct assay PubMed 14597732. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q64314-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q64314-2)

The sequence of this isoform differs from the canonical sequence as follows:
     322-325: GEDP → ELEP
     326-382: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 Potential
Chain35 – 382348Hematopoietic progenitor cell antigen CD34
PRO_0000020901

Regions

Topological domain35 – 287253Extracellular Potential
Transmembrane288 – 30821Helical; Potential
Topological domain309 – 38274Cytoplasmic Potential

Amino acid modifications

Modified residue3261Phosphotyrosine Ref.5
Modified residue3361Phosphotyrosine Ref.5
Glycosylation501N-linked (GlcNAc...) Potential
Glycosylation561N-linked (GlcNAc...) Potential
Glycosylation871N-linked (GlcNAc...) Potential
Glycosylation1371N-linked (GlcNAc...) Potential
Glycosylation1441N-linked (GlcNAc...) Potential
Glycosylation1451N-linked (GlcNAc...) Potential
Glycosylation2461N-linked (GlcNAc...) Ref.6

Natural variations

Alternative sequence322 – 3254GEDP → ELEP in isoform Short.
VSP_004161
Alternative sequence326 – 38257Missing in isoform Short.
VSP_004162

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EAE611ABE85C4957

FASTA38240,983
        10         20         30         40         50         60 
MQVHRDTRAG LLLPWRWVAL CLMSLLHLNN LTSATTETST QGISPSVPTN ESVEENITSS 

        70         80         90        100        110        120 
IPGSTSHYLI YQDSSKTTPA ISETMVNFTV TSGIPSGSGT PHTFSQPQTS PTGILPTTSD 

       130        140        150        160        170        180 
SISTSEMTWK SSLPSINVSD YSPNNSSFEM TSPTEPYAYT SSSAPSAIKG EIKCSGIREV 

       190        200        210        220        230        240 
RLAQGICLEL SEASSCEEFK KEKGEDLIQI LCEKEEAEAD AGASVCSLLL AQSEVRPECL 

       250        260        270        280        290        300 
LMVLANSTEL PSKLQLMEKH QSDLRKLGIQ SFNKQDIGSH QSYSRKTLIA LVTSGVLLAI 

       310        320        330        340        350        360 
LGTTGYFLMN RRSWSPTGER LGEDPYYTEN GGGQGYSSGP GASPETQGKA NVTRGAQENG 

       370        380 
TGQATSRNGH SARQHVVADT EL 

« Hide

Isoform Short [UniParc].

Checksum: 940BDA6660E65C4C
Show »

FASTA32535,263

References

« Hide 'large scale' references
[1]"The gene encoding the stem cell antigen, CD34, is conserved in mouse and expressed in haemopoietic progenitor cell lines, brain, and embryonic fibroblasts."
Brown J., Greaves M.F., Molgaard H.V.
Int. Immunol. 3:175-184(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG).
Strain: 129 and BALB/c.
Tissue: Bone marrow.
[2]"Two types of murine CD34 mRNA generated by alternative splicing."
Suda J., Sudo T., Ito M., Ohno N., Yamaguchi Y., Suda T.
Blood 79:2288-2295(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS LONG AND SHORT).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Strain: FVB/N.
Tissue: Mammary gland.
[4]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-326 AND TYR-336, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
[6]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-246.
Tissue: Myoblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S69293 mRNA. Translation: AAB19246.1.
S69302 Genomic DNA. No translation available.
S69295 Genomic DNA. No translation available.
S69299 mRNA. Translation: AAB22108.1. Sequence problems.
S69301 mRNA. Translation: AAB22109.1.
BC006607 mRNA. Translation: AAH06607.1.
PIRI65354.
RefSeqNP_598415.1. NM_133654.3.
UniGeneMm.29798.

3D structure databases

ProteinModelPortalQ64314.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ64314. 1 interaction.
MINTMINT-4090207.

PTM databases

PhosphoSiteQ64314.

Proteomic databases

PaxDbQ64314.
PRIDEQ64314.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000016638; ENSMUSP00000016638; ENSMUSG00000016494. [Q64314-1]
ENSMUST00000110815; ENSMUSP00000106439; ENSMUSG00000016494. [Q64314-2]
GeneID12490.
KEGGmmu:12490.
UCSCuc007eeq.2. mouse. [Q64314-1]

Organism-specific databases

CTD947.
MGIMGI:88329. Cd34.

Phylogenomic databases

eggNOGNOG42285.
GeneTreeENSGT00390000008414.
HOGENOMHOG000052513.
HOVERGENHBG003461.
InParanoidQ64314.
KOK06474.
OMAKGEIKCS.
OrthoDBEOG7CCBSQ.
PhylomeDBQ64314.
TreeFamTF335795.

Gene expression databases

ArrayExpressQ64314.
BgeeQ64314.
CleanExMM_CD34.
GenevestigatorQ64314.

Family and domain databases

InterProIPR008083. CD34.
IPR013836. CD34/Podocalyxin.
[Graphical view]
PANTHERPTHR16677. PTHR16677. 1 hit.
PfamPF06365. CD34_antigen. 1 hit.
[Graphical view]
PRINTSPR01700. CD34ANTIGEN.
ProtoNetSearch...

Other

NextBio281408.
PROQ64314.
SOURCESearch...

Entry information

Entry nameCD34_MOUSE
AccessionPrimary (citable) accession number: Q64314
Secondary accession number(s): Q62550, Q62551
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot