SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q64314

- CD34_MOUSE

UniProt

Q64314 - CD34_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Hematopoietic progenitor cell antigen CD34

Gene
Cd34
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins By similarity.

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB
  2. sulfate binding Source: MGI

GO - Biological processi

  1. cell proliferation Source: UniProtKB
  2. endothelial cell proliferation Source: Ensembl
  3. endothelium development Source: Ensembl
  4. extracellular vesicular exosome assembly Source: Ensembl
  5. glomerular endothelium development Source: Ensembl
  6. glomerular filtration Source: UniProtKB
  7. glutamate metabolic process Source: UniProtKB
  8. hematopoietic stem cell proliferation Source: Ensembl
  9. leukocyte migration Source: MGI
  10. mesangial cell-matrix adhesion Source: UniProtKB
  11. metanephric glomerular mesangial cell differentiation Source: Ensembl
  12. negative regulation of blood coagulation Source: UniProtKB
  13. negative regulation of cellular response to heat Source: UniProtKB
  14. negative regulation of cellular response to hypoxia Source: UniProtKB
  15. negative regulation of gene expression Source: Ensembl
  16. negative regulation of interleukin-2 secretion Source: Ensembl
  17. negative regulation of necrotic cell death Source: Ensembl
  18. negative regulation of neuron death Source: UniProtKB
  19. negative regulation of nitric oxide biosynthetic process Source: UniProtKB
  20. negative regulation of tumor necrosis factor production Source: UniProtKB
  21. paracrine signaling Source: Ensembl
  22. positive regulation of angiogenesis Source: UniProtKB
  23. positive regulation of gene expression Source: Ensembl
  24. positive regulation of glial cell line-derived neurotrophic factor secretion Source: Ensembl
  25. positive regulation of granulocyte colony-stimulating factor production Source: Ensembl
  26. positive regulation of interleukin-10 production Source: UniProtKB
  27. positive regulation of odontogenesis Source: UniProtKB
  28. positive regulation of transforming growth factor beta production Source: UniProtKB
  29. positive regulation of vasculogenesis Source: UniProtKB
  30. regulation of blood pressure Source: UniProtKB
  31. signal transduction Source: Ensembl
  32. single organismal cell-cell adhesion Source: UniProtKB
  33. tissue homeostasis Source: UniProtKB
  34. transdifferentiation Source: Ensembl
  35. vascular wound healing Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
Hematopoietic progenitor cell antigen CD34
Alternative name(s):
CD_antigen: CD34
Gene namesi
Name:Cd34
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:88329. Cd34.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini35 – 287253Extracellular Reviewed predictionAdd
BLAST
Transmembranei288 – 30821Helical; Reviewed predictionAdd
BLAST
Topological domaini309 – 38274Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB
  2. basal plasma membrane Source: UniProtKB
  3. cell periphery Source: MGI
  4. cell surface Source: UniProtKB
  5. cytoplasm Source: MGI
  6. external side of plasma membrane Source: MGI
  7. extracellular region Source: MGI
  8. glomerular endothelium fenestra Source: UniProtKB
  9. intercellular bridge Source: UniProtKB
  10. lysosome Source: UniProtKB
  11. membrane Source: MGI
  12. perinuclear region of cytoplasm Source: UniProtKB
  13. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434 Reviewed predictionAdd
BLAST
Chaini35 – 382348Hematopoietic progenitor cell antigen CD34PRO_0000020901Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi50 – 501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi56 – 561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi87 – 871N-linked (GlcNAc...) Reviewed prediction
Glycosylationi137 – 1371N-linked (GlcNAc...) Reviewed prediction
Glycosylationi144 – 1441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi145 – 1451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi246 – 2461N-linked (GlcNAc...)1 Publication
Modified residuei326 – 3261Phosphotyrosine1 Publication
Modified residuei336 – 3361Phosphotyrosine1 Publication

Post-translational modificationi

Highly glycosylated By similarity.
Phosphorylated on serine residues by PKC By similarity.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ64314.
PaxDbiQ64314.
PRIDEiQ64314.

PTM databases

PhosphoSiteiQ64314.

Expressioni

Tissue specificityi

Highly expressed in hematopoietic progenitor cell lines, brain and testis, and moderately in the thymus, spleen, and bone marrow, but not in adult liver.

Gene expression databases

ArrayExpressiQ64314.
BgeeiQ64314.
CleanExiMM_CD34.
GenevestigatoriQ64314.

Interactioni

Protein-protein interaction databases

IntActiQ64314. 1 interaction.
MINTiMINT-4090207.

Structurei

3D structure databases

ProteinModelPortaliQ64314.

Family & Domainsi

Sequence similaritiesi

Belongs to the CD34 family.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG42285.
GeneTreeiENSGT00390000008414.
HOGENOMiHOG000052513.
HOVERGENiHBG003461.
InParanoidiQ64314.
KOiK06474.
OMAiKGEIKCS.
OrthoDBiEOG7CCBSQ.
PhylomeDBiQ64314.
TreeFamiTF335795.

Family and domain databases

InterProiIPR008083. CD34.
IPR013836. CD34/Podocalyxin.
[Graphical view]
PANTHERiPTHR16677. PTHR16677. 1 hit.
PfamiPF06365. CD34_antigen. 1 hit.
[Graphical view]
PRINTSiPR01700. CD34ANTIGEN.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: Q64314-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MQVHRDTRAG LLLPWRWVAL CLMSLLHLNN LTSATTETST QGISPSVPTN    50
ESVEENITSS IPGSTSHYLI YQDSSKTTPA ISETMVNFTV TSGIPSGSGT 100
PHTFSQPQTS PTGILPTTSD SISTSEMTWK SSLPSINVSD YSPNNSSFEM 150
TSPTEPYAYT SSSAPSAIKG EIKCSGIREV RLAQGICLEL SEASSCEEFK 200
KEKGEDLIQI LCEKEEAEAD AGASVCSLLL AQSEVRPECL LMVLANSTEL 250
PSKLQLMEKH QSDLRKLGIQ SFNKQDIGSH QSYSRKTLIA LVTSGVLLAI 300
LGTTGYFLMN RRSWSPTGER LGEDPYYTEN GGGQGYSSGP GASPETQGKA 350
NVTRGAQENG TGQATSRNGH SARQHVVADT EL 382
Length:382
Mass (Da):40,983
Last modified:November 1, 1996 - v1
Checksum:iEAE611ABE85C4957
GO
Isoform Short (identifier: Q64314-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     322-325: GEDP → ELEP
     326-382: Missing.

Show »
Length:325
Mass (Da):35,263
Checksum:i940BDA6660E65C4C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei322 – 3254GEDP → ELEP in isoform Short. VSP_004161
Alternative sequencei326 – 38257Missing in isoform Short. VSP_004162Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S69293 mRNA. Translation: AAB19246.1.
S69302 Genomic DNA. No translation available.
S69295 Genomic DNA. No translation available.
S69299 mRNA. Translation: AAB22108.1. Sequence problems.
S69301 mRNA. Translation: AAB22109.1.
BC006607 mRNA. Translation: AAH06607.1.
CCDSiCCDS15640.1. [Q64314-1]
CCDS48490.1. [Q64314-2]
PIRiI65354.
RefSeqiNP_598415.1. NM_133654.3. [Q64314-1]
UniGeneiMm.29798.

Genome annotation databases

EnsembliENSMUST00000016638; ENSMUSP00000016638; ENSMUSG00000016494. [Q64314-1]
ENSMUST00000110815; ENSMUSP00000106439; ENSMUSG00000016494. [Q64314-2]
GeneIDi12490.
KEGGimmu:12490.
UCSCiuc007eeq.2. mouse. [Q64314-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S69293 mRNA. Translation: AAB19246.1 .
S69302 Genomic DNA. No translation available.
S69295 Genomic DNA. No translation available.
S69299 mRNA. Translation: AAB22108.1 . Sequence problems.
S69301 mRNA. Translation: AAB22109.1 .
BC006607 mRNA. Translation: AAH06607.1 .
CCDSi CCDS15640.1. [Q64314-1 ]
CCDS48490.1. [Q64314-2 ]
PIRi I65354.
RefSeqi NP_598415.1. NM_133654.3. [Q64314-1 ]
UniGenei Mm.29798.

3D structure databases

ProteinModelPortali Q64314.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q64314. 1 interaction.
MINTi MINT-4090207.

PTM databases

PhosphoSitei Q64314.

Proteomic databases

MaxQBi Q64314.
PaxDbi Q64314.
PRIDEi Q64314.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000016638 ; ENSMUSP00000016638 ; ENSMUSG00000016494 . [Q64314-1 ]
ENSMUST00000110815 ; ENSMUSP00000106439 ; ENSMUSG00000016494 . [Q64314-2 ]
GeneIDi 12490.
KEGGi mmu:12490.
UCSCi uc007eeq.2. mouse. [Q64314-1 ]

Organism-specific databases

CTDi 947.
MGIi MGI:88329. Cd34.

Phylogenomic databases

eggNOGi NOG42285.
GeneTreei ENSGT00390000008414.
HOGENOMi HOG000052513.
HOVERGENi HBG003461.
InParanoidi Q64314.
KOi K06474.
OMAi KGEIKCS.
OrthoDBi EOG7CCBSQ.
PhylomeDBi Q64314.
TreeFami TF335795.

Enzyme and pathway databases

Reactomei REACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Miscellaneous databases

NextBioi 281408.
PROi Q64314.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q64314.
Bgeei Q64314.
CleanExi MM_CD34.
Genevestigatori Q64314.

Family and domain databases

InterProi IPR008083. CD34.
IPR013836. CD34/Podocalyxin.
[Graphical view ]
PANTHERi PTHR16677. PTHR16677. 1 hit.
Pfami PF06365. CD34_antigen. 1 hit.
[Graphical view ]
PRINTSi PR01700. CD34ANTIGEN.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The gene encoding the stem cell antigen, CD34, is conserved in mouse and expressed in haemopoietic progenitor cell lines, brain, and embryonic fibroblasts."
    Brown J., Greaves M.F., Molgaard H.V.
    Int. Immunol. 3:175-184(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG).
    Strain: 129 and BALB/c.
    Tissue: Bone marrow.
  2. "Two types of murine CD34 mRNA generated by alternative splicing."
    Suda J., Sudo T., Ito M., Ohno N., Yamaguchi Y., Suda T.
    Blood 79:2288-2295(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS LONG AND SHORT).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-326 AND TYR-336, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  6. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-246.
    Tissue: Myoblast.

Entry informationi

Entry nameiCD34_MOUSE
AccessioniPrimary (citable) accession number: Q64314
Secondary accession number(s): Q62550, Q62551
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi