Q64311 (NTAN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein N-terminal asparagine amidohydrolase
EC=3.5.1.-

Alternative name(s):
Protein NH2-terminal asparagine amidohydrolase
Short name=PNAA
Protein NH2-terminal asparagine deamidase
Short name=PNAD
Short name=Protein N-terminal Asn amidase
Short name=Protein NTN-amidase

Gene names

Name:

Ntan1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	adult locomotory behavior Inferred from mutant phenotype. Source: MGI memory Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	protein-N-terminal asparagine amidohydrolase activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 310	309	Protein N-terminal asparagine amidohydrolase		PRO_0000057972

Sequences

	Sequence		Length	Mass (Da)
	Q64311 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 09B1611DF3366959	FASTA	310	34,595
	10 20 30 40 50 60 MPLLVDGQRV RLPRSAVELV RAHPPLEERA RLLRGQSVQQ VGPQGLLYVQ QRELAVTSPK 70 80 90 100 110 120 DGSISILGSD DATTCHIVVL RHTGNGATCL THCDGSDTKA EVPLIMSSIK SFSEHAECGR 130 140 150 160 170 180 LEVHLVGGFS DDRQLSQKLT HQLLSEFDKQ DDDIHLVTLC VTELNDREEN ENHFPIIYGI 190 200 210 220 230 240 AVNIKTAEIY RASFQDRGPE EQLRAARALA GGPMISIYDA KTEQLRIGPC SWTPFPQVDF 250 260 270 280 290 300 WLQQDDKQIL ESLSTSPLAE PPHFVEHIRS TLMFLKKFPS PENILFPGNK ALLYKKNKDG 310 LWEKISSPGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A mouse amidase specific for N-terminal asparagine. The gene, the enzyme, and their function in the N-end rule pathway." Grigoryev S., Stewart A.E., Kwon Y.T., Arfin S.M., Bradshaw R.A., Jenkins N.A., Copeland N.G., Varshavsky A. J. Biol. Chem. 271:28521-28532(1996) [PubMed: 8910481] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: C129.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U57692 mRNA. Translation: AAB66490.1. U57691 Genomic DNA. Translation: AAC52885.1. BC030172 mRNA. Translation: AAH30172.1.
IPI	IPI00224124.
RefSeq	NP_035076.1. NM_010946.3.
UniGene	Mm.380410.
3D structure databases
ProteinModelPortal	Q64311.
ModBase	Search...
Protein-protein interaction databases
STRING	Q64311.
PTM databases
PhosphoSite	Q64311.
Proteomic databases
PRIDE	Q64311.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023362; ENSMUSP00000023362; ENSMUSG00000022681.
GeneID	18203.
KEGG	mmu:18203.
UCSC	uc007ygk.1. mouse.
Organism-specific databases
CTD	123803.
MGI	MGI:108471. Ntan1.
Phylogenomic databases
eggNOG	roNOG04619.
HOGENOM	HBG717669.
HOVERGEN	HBG052674.
InParanoid	Q64311.
OMA	CHIVVLR.
OrthoDB	EOG4FJ89K.
PhylomeDB	Q64311.
Gene expression databases
ArrayExpress	Q64311.
Bgee	Q64311.
CleanEx	MM_NTAN1.
Genevestigator	Q64311.
GermOnline	ENSMUSG00000022681. Mus musculus.
Family and domain databases
KO	K14662.
ProtoNet	Search...
Other
NextBio	293578.
SOURCE	Search...

Entry information

Entry name

NTAN1_MOUSE

Accession

Primary (citable) accession number: Q64311

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 70 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program