Reviewed, UniProtKB/Swiss-Prot Q64311 (NTAN1_MOUSE)

Names and origin

Protein names

Recommended name:
    Protein N-terminal asparagine amidohydrolase
    EC=3.5.1.-
Alternative name(s):
    Protein NH2-terminal asparagine deamidase
      Short name=PNAD
      Short name=N-terminal Asn amidase
      Short name=NTN-amidase
    Protein NH2-terminal asparagine amidohydrolase
      Short name=PNAA

Gene names

Name:

Ntan1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	adult locomotory behavior Inferred from mutant phenotype. Source: MGI memory Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	protein N-terminal asparagine amidohydrolase activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 310	309	Protein N-terminal asparagine amidohydrolase		PRO_0000057972

Sequences

	Sequence		Length	Mass (Da)
	Q64311-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 09B1611DF3366959	FASTA	310	34,595
	10 20 30 40 50 60 MPLLVDGQRV RLPRSAVELV RAHPPLEERA RLLRGQSVQQ VGPQGLLYVQ QRELAVTSPK 70 80 90 100 110 120 DGSISILGSD DATTCHIVVL RHTGNGATCL THCDGSDTKA EVPLIMSSIK SFSEHAECGR 130 140 150 160 170 180 LEVHLVGGFS DDRQLSQKLT HQLLSEFDKQ DDDIHLVTLC VTELNDREEN ENHFPIIYGI 190 200 210 220 230 240 AVNIKTAEIY RASFQDRGPE EQLRAARALA GGPMISIYDA KTEQLRIGPC SWTPFPQVDF 250 260 270 280 290 300 WLQQDDKQIL ESLSTSPLAE PPHFVEHIRS TLMFLKKFPS PENILFPGNK ALLYKKNKDG 310 LWEKISSPGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A mouse amidase specific for N-terminal asparagine. The gene, the enzyme, and their function in the N-end rule pathway." Grigoryev S., Stewart A.E., Kwon Y.T., Arfin S.M., Bradshaw R.A., Jenkins N.A., Copeland N.G., Varshavsky A. J. Biol. Chem. 271:28521-28532(1996) [PubMed: 8910481] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: C129.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U57692 mRNA. Translation: AAB66490.1. U57691 Genomic DNA. Translation: AAC52885.1. BC030172 mRNA. Translation: AAH30172.1.
IPI	IPI00224124.
RefSeq	NP_035076.1.
UniGene	Mm.380410
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000022681. Mus musculus. [Contig view]
GeneID	18203.
KEGG	mmu:18203.
Organism-specific databases
MGI	MGI:108471. Ntan1.
Phylogenomic databases
HOGENOM	Q64311.
HOVERGEN	Q64311.
OMA	Q64311. GNKALLY.
Gene expression databases
ArrayExpress	Q64311.
Bgee	Q64311.
CleanEx	MM_NTAN1.
GermOnline	ENSMUSG00000022681. Mus musculus.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	293578.
SOURCE	Search...

Entry information

Entry name

NTAN1_MOUSE

Accession

Primary (citable) accession number: Q64311

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 50 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)