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Protein

Pancreas transcription factor 1 subunit alpha

Gene

Ptf1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreas transcription factor 1 subunit alpha
Alternative name(s):
Pancreas-specific transcription factor 1a
bHLH transcription factor p48
p48 DNA-binding subunit of transcription factor PTF1
Short name:
PTF1-p48
Gene namesi
Name:Ptf1a
Synonyms:Ptf1p48
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi621709. Ptf1a.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication
  • Cytoplasm 1 Publication

  • Note: In the cytoplasm loses its ability to form the PTF1 complex.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Pancreas transcription factor 1 subunit alphaPRO_0000233145Add
BLAST

Proteomic databases

PaxDbiQ64305.
PRIDEiQ64305.

Expressioni

Tissue specificityi

Exocrine pancreas-specific. Expressed in azaserine-induced pancreatic tumors (at protein level). Expressed in AR42J cells but not in ARIP, DSL6A, or DSL6B cells. Down-regulation is associated with the change of an azaserine-induced acinar cell carcinoma to a ductal phenotype.1 Publication

Developmental stagei

Expressed from day 12 of gestation in pancreatic structures.1 Publication

Gene expression databases

GenevisibleiQ64305. RN.

Interactioni

Subunit structurei

Component of the pancreas transcription factor 1 complex (PTF1) which is composed of TCF3/p75, TCF12/p64 and PTF1A/p48. TCF3 is responsible for the nuclear import of the p48/p64 complex. Interacts with TCF3 and RBPSUH/RBP-Jkappa (By similarity).By similarity

Protein-protein interaction databases

BioGridi250633. 1 interaction.
STRINGi10116.ENSRNOP00000022727.

Structurei

3D structure databases

ProteinModelPortaliQ64305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini162 – 21453bHLHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi140 – 1489Poly-Ala

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00730000110958.
HOGENOMiHOG000231913.
HOVERGENiHBG082224.
InParanoidiQ64305.
KOiK09073.
OMAiHEYCYRD.
OrthoDBiEOG091G0RR2.
PhylomeDBiQ64305.
TreeFamiTF315153.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64305-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAVLLEHFP GALDTFPSSY FDEEDFFTDQ SSRDPLEDGD ELLGDEQAEV
60 70 80 90 100
EFLSHQLHEY CYRDGACLLL QPAPSAAPHA LAPPPLGDPG EPEDSGSYCC
110 120 130 140 150
DAGAPLGAFP YSPGSPPSCL AYPCTAVLSP GTRLRGLNGA AAAAAAAARR
160 170 180 190 200
RRRVRSEAEL QQLRQAANVR ERRRMQSIND AFEGLRSHIP TLPYEKRLSK
210 220 230 240 250
VDTLRLAIGY INFLSELVQA DLPLRGSGTG GCGGPGGSRH LGGDSPGNQA
260 270 280 290 300
QKVIICHRGT RSPSPSDPDY GLPPLAGHSL SWADEKQLKE QNIIRTAKVW
310 320
TPEDPRKLNS KSFDNIENEP PFEFVS
Length:326
Mass (Da):35,305
Last modified:November 1, 1996 - v1
Checksum:iE06D0132390E429F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98170 mRNA. Translation: CAA66851.1.
X98446 Genomic DNA. Translation: CAA67076.1.
PIRiS71755.
RefSeqiNP_446416.1. NM_053964.1.
UniGeneiRn.10536.

Genome annotation databases

EnsembliENSRNOT00000022727; ENSRNOP00000022727; ENSRNOG00000016902.
GeneIDi117034.
KEGGirno:117034.
UCSCiRGD:621709. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98170 mRNA. Translation: CAA66851.1.
X98446 Genomic DNA. Translation: CAA67076.1.
PIRiS71755.
RefSeqiNP_446416.1. NM_053964.1.
UniGeneiRn.10536.

3D structure databases

ProteinModelPortaliQ64305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250633. 1 interaction.
STRINGi10116.ENSRNOP00000022727.

Proteomic databases

PaxDbiQ64305.
PRIDEiQ64305.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022727; ENSRNOP00000022727; ENSRNOG00000016902.
GeneIDi117034.
KEGGirno:117034.
UCSCiRGD:621709. rat.

Organism-specific databases

CTDi256297.
RGDi621709. Ptf1a.

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00730000110958.
HOGENOMiHOG000231913.
HOVERGENiHBG082224.
InParanoidiQ64305.
KOiK09073.
OMAiHEYCYRD.
OrthoDBiEOG091G0RR2.
PhylomeDBiQ64305.
TreeFamiTF315153.

Miscellaneous databases

PROiQ64305.

Gene expression databases

GenevisibleiQ64305. RN.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTF1A_RAT
AccessioniPrimary (citable) accession number: Q64305
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.