Q642A5 (DPOE3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase epsilon subunit 3
EC=2.7.7.7

Alternative name(s):
DNA polymerase II subunit 3
DNA polymerase epsilon subunit p17

Gene names

Name:

Pole3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1 By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. Interaction with POLE4 is a prerequisite for further binding with POLE and POLE2. Interacts with CHRAC1. Together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRAC By similarity.
Subcellular location	Nucleus Potential.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Coiled coil
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	epsilon DNA polymerase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 145	144	DNA polymerase epsilon subunit 3		PRO_0000208344
Regions
	Coiled coil	85 – 144	60	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	83	1	Phosphothreonine By similarity
	Modified residue	121	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q642A5 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 6CB14ED20FE15EC1	FASTA	145	16,674
	10 20 30 40 50 60 MAERPEDLNL PNAVITRIIK EALPDGVNIS KEARSAISRA ASVFVLYATS CANNFAMKGK 70 80 90 100 110 120 RKTLNASDVL SAMEEMEFQR FVTPLKEALE AYRREQKGKK EASEQKKKDK DKKDCEEQDK 130 140 SREEEDEDEE RLDEEEQNEE EEVDN

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC081988 mRNA. Translation: AAH81988.1. BC083800 mRNA. Translation: AAH83800.1.
IPI	IPI00208715.
RefSeq	NP_001007653.1. NM_001007652.2. XP_002726991.1. XM_002726945.2. XP_002729875.1. XM_002729829.2.
UniGene	Rn.3290.
3D structure databases
ProteinModelPortal	Q642A5.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000020722.
Proteomic databases
PRIDE	Q642A5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000006416; ENSRNOP00000006416; ENSRNOG00000004843. ENSRNOT00000020722; ENSRNOP00000020722; ENSRNOG00000015392.
GeneID	100362333. 298098.
KEGG	rno:100362333. rno:298098.
UCSC	RGD:1359475. rat.
Organism-specific databases
CTD	54107.
RGD	1359475. Pole3.
Phylogenomic databases
eggNOG	COG2036.
GeneTree	ENSGT00550000074689.
HOGENOM	HOG000007701.
HOVERGEN	HBG060262.
InParanoid	Q642A5.
KO	K02326.
OMA	NDSGGHK.
OrthoDB	EOG4TB4CS.
Gene expression databases
Genevestigator	Q642A5.
GermOnline	ENSRNOG00000004843. Rattus norvegicus. ENSRNOG00000015392. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.20.10. 1 hit.
InterPro	IPR003958. CBFA_NFYB_domain. IPR009072. Histone-fold. [Graphical view]
Pfam	PF00808. CBFD_NFYB_HMF. 1 hit. [Graphical view]
SUPFAM	SSF47113. Histone-fold. 1 hit.
ProtoNet	Search...
Other
NextBio	643156.

Entry information

Entry name

DPOE3_RAT

Accession

Primary (citable) accession number: Q642A5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	October 25, 2004
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program