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Protein

DNA polymerase epsilon subunit 3

Gene

Pole3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit 3 (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit 3
DNA polymerase epsilon subunit p17
Gene namesi
Name:Pole3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componentsi: Chromosome 7, Unassembled WGS sequence

Organism-specific databases

RGDi1359475. Pole3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 145144DNA polymerase epsilon subunit 3PRO_0000208344Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei83 – 831PhosphothreonineBy similarity
Modified residuei121 – 1211PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ642A5.

Expressioni

Gene expression databases

GenevisibleiQ642A5. RN.

Interactioni

Subunit structurei

Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. Interaction with POLE4 is a prerequisite for further binding with POLE and POLE2. Interacts with CHRAC1. Together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRAC (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020722.

Structurei

3D structure databases

ProteinModelPortaliQ642A5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili85 – 14460Sequence AnalysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG2036.
GeneTreeiENSGT00550000074689.
HOGENOMiHOG000007701.
HOVERGENiHBG060262.
InParanoidiQ642A5.
KOiK02326.
OMAiQAASVFV.
OrthoDBiEOG7RNK2W.
PhylomeDBiQ642A5.
TreeFamiTF103008.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q642A5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAERPEDLNL PNAVITRIIK EALPDGVNIS KEARSAISRA ASVFVLYATS
60 70 80 90 100
CANNFAMKGK RKTLNASDVL SAMEEMEFQR FVTPLKEALE AYRREQKGKK
110 120 130 140
EASEQKKKDK DKKDCEEQDK SREEEDEDEE RLDEEEQNEE EEVDN
Length:145
Mass (Da):16,674
Last modified:October 25, 2004 - v1
Checksum:i6CB14ED20FE15EC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081988 mRNA. Translation: AAH81988.1.
BC083800 mRNA. Translation: AAH83800.1.
RefSeqiNP_001007653.1. NM_001007652.2.
XP_002726991.1. XM_002726945.4.
XP_002729875.1. XM_002729829.4.
UniGeneiRn.3290.

Genome annotation databases

EnsembliENSRNOT00000006416; ENSRNOP00000006416; ENSRNOG00000004843.
ENSRNOT00000089792; ENSRNOP00000070346; ENSRNOG00000055277.
GeneIDi100362333.
298098.
KEGGirno:100362333.
rno:298098.
UCSCiRGD:1359475. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081988 mRNA. Translation: AAH81988.1.
BC083800 mRNA. Translation: AAH83800.1.
RefSeqiNP_001007653.1. NM_001007652.2.
XP_002726991.1. XM_002726945.4.
XP_002729875.1. XM_002729829.4.
UniGeneiRn.3290.

3D structure databases

ProteinModelPortaliQ642A5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020722.

Proteomic databases

PRIDEiQ642A5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000006416; ENSRNOP00000006416; ENSRNOG00000004843.
ENSRNOT00000089792; ENSRNOP00000070346; ENSRNOG00000055277.
GeneIDi100362333.
298098.
KEGGirno:100362333.
rno:298098.
UCSCiRGD:1359475. rat.

Organism-specific databases

CTDi54107.
RGDi1359475. Pole3.

Phylogenomic databases

eggNOGiCOG2036.
GeneTreeiENSGT00550000074689.
HOGENOMiHOG000007701.
HOVERGENiHBG060262.
InParanoidiQ642A5.
KOiK02326.
OMAiQAASVFV.
OrthoDBiEOG7RNK2W.
PhylomeDBiQ642A5.
TreeFamiTF103008.

Miscellaneous databases

NextBioi643156.
PROiQ642A5.

Gene expression databases

GenevisibleiQ642A5. RN.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiDPOE3_RAT
AccessioniPrimary (citable) accession number: Q642A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: October 25, 2004
Last modified: July 22, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.