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Protein

Podoplanin

Gene

Pdpn

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels (By similarity).By similarity

GO - Biological processi

  • cell adhesion Source: MGI
  • cell morphogenesis Source: UniProtKB
  • inflammatory response Source: RGD
  • lung development Source: UniProtKB
  • lymphangiogenesis Source: UniProtKB
  • nervous system development Source: RGD
  • positive regulation of cell migration Source: MGI
  • positive regulation of cellular component movement Source: UniProtKB
  • positive regulation of platelet activation Source: RGD
  • regulation of cell shape Source: UniProtKB-KW
  • response to hyperoxia Source: RGD
  • tube morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell shape

Keywords - Ligandi

Sialic acid

Names & Taxonomyi

Protein namesi
Recommended name:
Podoplanin
Alternative name(s):
E11 antigen epitope
Glycoprotein 38
Short name:
Gp38
OTS-8
RTI140
T1-alpha
Short name:
T1A
Type I cell 40 kDa protein
Gene namesi
Name:Pdpn
Synonyms:Gp38, Ots8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61819. Pdpn.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 135113ExtracellularSequence analysisAdd
BLAST
Transmembranei136 – 15621HelicalSequence analysisAdd
BLAST
Topological domaini157 – 16610CytoplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 166144PodoplaninPRO_0000021353Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341O-linked (GalNAc...)Sequence analysis
Glycosylationi52 – 521O-linked (GalNAc...)Sequence analysis
Glycosylationi55 – 551O-linked (GalNAc...)Sequence analysis
Glycosylationi56 – 561O-linked (GalNAc...)Sequence analysis
Glycosylationi62 – 621O-linked (GalNAc...)Sequence analysis
Glycosylationi63 – 631O-linked (GalNAc...)Sequence analysis
Glycosylationi71 – 711O-linked (GalNAc...)Sequence analysis
Glycosylationi80 – 801O-linked (GalNAc...)Sequence analysis
Glycosylationi81 – 811O-linked (GalNAc...)Sequence analysis
Glycosylationi83 – 831O-linked (GalNAc...)Sequence analysis
Glycosylationi84 – 841O-linked (GalNAc...)Sequence analysis
Glycosylationi94 – 941O-linked (GalNAc...)Sequence analysis
Glycosylationi95 – 951O-linked (GalNAc...)Sequence analysis
Glycosylationi96 – 961O-linked (GalNAc...)Sequence analysis
Glycosylationi101 – 1011O-linked (GalNAc...)Sequence analysis
Glycosylationi105 – 1051O-linked (GalNAc...)Sequence analysis
Glycosylationi109 – 1091O-linked (GalNAc...)Sequence analysis
Glycosylationi110 – 1101O-linked (GalNAc...)Sequence analysis

Post-translational modificationi

Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.1 Publication
The N-terminus is blocked.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ64294.
PRIDEiQ64294.

Expressioni

Tissue specificityi

In adult kidney, expressed on the urinary surface and foot processes of podocytes and in parietal epithelial cells of Bowman's capsule where it is localized to luminal surfaces. In lung, expressed exclusively on luminal surfaces of type I alveolar epithelial cells and pleural mesothelial cells. Not expressed in type II alveolar cells. In bone, expressed in osteocytes and osteoblasts. In spleen, liver, stomach and intestine, expressed in mesoepithelium. Also expressed in thymic epithelial cells, choroid plexus and leptomeninges.2 Publications

Developmental stagei

In newborn kidney, not detected at the vesicle stage. First detected in S-shaped bodies.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020316.

Family & Domainsi

Sequence similaritiesi

Belongs to the podoplanin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J49G. Eukaryota.
ENOG41116TP. LUCA.
HOGENOMiHOG000231122.
HOVERGENiHBG080131.
InParanoidiQ64294.
KOiK16778.
OrthoDBiEOG7DC25R.
PhylomeDBiQ64294.
TreeFamiTF337068.

Family and domain databases

InterProiIPR008783. Podoplanin.
[Graphical view]
PfamiPF05808. Podoplanin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWTAPVLLWV LGSVWFWDSA QGGAIGALED DLVTPGPGDD MVNPGLEDRI
60 70 80 90 100
ETTDTTGELD KSTAKAPLVP TQPPIEELPT SGTSDHDHKE HESTTTVKAV
110 120 130 140 150
TSHSTDKKTT HPNRDNAGGE TQTTDKKDGL AVVTLVGIII GVLLAIGFIG
160
GIIIVVMRKI SGRFSP
Length:166
Mass (Da):17,579
Last modified:November 1, 1996 - v1
Checksum:iB97C58292B664468
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti112 – 1121P → S (PubMed:9327748).Curated
Sequence conflicti112 – 1121P → S (PubMed:15489334).Curated
Sequence conflicti135 – 1373LVG → WSA in AAB93880 (PubMed:9761764).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07797 mRNA. Translation: AAA92789.1.
U32115 mRNA. Translation: AAA74431.1.
U96449 mRNA. Translation: AAB86438.1.
U92440, U92081 Genomic DNA. Translation: AAB93880.1.
BC072492 mRNA. Translation: AAH72492.1.
RefSeqiNP_062231.1. NM_019358.1.
UniGeneiRn.794.

Genome annotation databases

GeneIDi54320.
KEGGirno:54320.
UCSCiRGD:61819. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07797 mRNA. Translation: AAA92789.1.
U32115 mRNA. Translation: AAA74431.1.
U96449 mRNA. Translation: AAB86438.1.
U92440, U92081 Genomic DNA. Translation: AAB93880.1.
BC072492 mRNA. Translation: AAH72492.1.
RefSeqiNP_062231.1. NM_019358.1.
UniGeneiRn.794.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020316.

Proteomic databases

PaxDbiQ64294.
PRIDEiQ64294.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi54320.
KEGGirno:54320.
UCSCiRGD:61819. rat.

Organism-specific databases

CTDi10630.
RGDi61819. Pdpn.

Phylogenomic databases

eggNOGiENOG410J49G. Eukaryota.
ENOG41116TP. LUCA.
HOGENOMiHOG000231122.
HOVERGENiHBG080131.
InParanoidiQ64294.
KOiK16778.
OrthoDBiEOG7DC25R.
PhylomeDBiQ64294.
TreeFamiTF337068.

Miscellaneous databases

PROiQ64294.

Family and domain databases

InterProiIPR008783. Podoplanin.
[Graphical view]
PfamiPF05808. Podoplanin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, characterization, and development expression of a rat lung alveolar type I cell gene in embryonic endodermal and neural derivatives."
    Rishi A.K., Joyce-Brady M., Fisher J., Dobbs L.G., Floros J., Vanderspek J., Brody J.S., Williams M.C.
    Dev. Biol. 167:294-306(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Lung.
  2. "Characterization and cloning of the E11 antigen, a marker expressed by rat osteoblasts and osteocytes."
    Wetterwald A., Hoffstetter W., Cecchini M.G., Lanske B., Wagner C., Fleisch H., Atkinson M.
    Bone 18:125-132(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Osteosarcoma.
  3. "Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis."
    Breiteneder-Geleff S., Matsui K., Soleiman A., Meraner P., Poczewski H., Kalt R., Schaffner G., Kerjaschki D.
    Am. J. Pathol. 151:1141-1152(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DOWN-REGULATION IN PAN.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  4. "Characterization of the gene and promoter for RTI40, a differentiation marker of type I alveolar epithelial cells."
    Vanderbilt J.N., Dobbs L.G.
    Am. J. Respir. Cell Mol. Biol. 19:662-671(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  6. "Purification and analysis of RTI40, a type I alveolar epithelial cell apical membrane protein."
    Gonzalez R.F., Dobbs L.G.
    Biochim. Biophys. Acta 1429:208-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 41-55, GLYCOSYLATION, BLOCKAGE OF N-TERMINUS.

Entry informationi

Entry nameiPDPN_RAT
AccessioniPrimary (citable) accession number: Q64294
Secondary accession number(s): O08731, O55210
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Down-regulated in puromycin aminonucleoside nephrosis (PAN).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.