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Q64289

- NDF1_RAT

UniProt

Q64289 - NDF1_RAT

Protein

Neurogenic differentiation factor 1

Gene

Neurod1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis By similarity.By similarity

    GO - Molecular functioni

    1. chromatin binding Source: UniProtKB
    2. double-stranded DNA binding Source: RGD
    3. E-box binding Source: Ensembl
    4. RNA polymerase II transcription coactivator activity Source: Ensembl
    5. sequence-specific DNA binding Source: Ensembl
    6. sequence-specific DNA binding transcription factor activity Source: Ensembl

    GO - Biological processi

    1. amacrine cell differentiation Source: UniProtKB
    2. anterior/posterior pattern specification Source: Ensembl
    3. cellular response to glucose stimulus Source: UniProtKB
    4. cerebellum development Source: RGD
    5. dentate gyrus development Source: UniProtKB
    6. embryonic organ morphogenesis Source: Ensembl
    7. endocrine pancreas development Source: UniProtKB
    8. enteroendocrine cell differentiation Source: UniProtKB
    9. inner ear development Source: UniProtKB
    10. insulin secretion Source: Ensembl
    11. negative regulation of JAK-STAT cascade Source: Ensembl
    12. negative regulation of type B pancreatic cell apoptotic process Source: Ensembl
    13. nitric oxide mediated signal transduction Source: Ensembl
    14. nucleocytoplasmic transport Source: UniProtKB
    15. pancreatic A cell fate commitment Source: Ensembl
    16. pancreatic PP cell fate commitment Source: Ensembl
    17. positive regulation of apoptotic process Source: UniProtKB
    18. positive regulation of cell differentiation Source: UniProtKB
    19. positive regulation of neuron differentiation Source: RGD
    20. positive regulation of sequence-specific DNA binding transcription factor activity Source: Ensembl
    21. positive regulation of transcription, DNA-templated Source: UniProtKB
    22. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    23. positive regulation of transcription regulatory region DNA binding Source: Ensembl
    24. regulation of cell cycle arrest Source: UniProtKB
    25. regulation of intestinal epithelial structure maintenance Source: UniProtKB
    26. response to drug Source: RGD
    27. signal transduction involved in regulation of gene expression Source: Ensembl

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Differentiation, Neurogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neurogenic differentiation factor 1
    Short name:
    NeuroD1
    Alternative name(s):
    Basic helix-loop-helix factor 1
    Short name:
    BHF-1
    Gene namesi
    Name:Neurod1
    Synonyms:Neurod
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi3165. Neurod1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 PublicationPROSITE-ProRule annotation
    Note: Colocalizes with NR0B2 in the nucleus By similarity. In pancreatic islet cells, shuttles to the nucleus in response to glucose stimulation.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi259 – 2591S → A: No effect on subcellular location. 1 Publication
    Mutagenesisi262 – 2621T → A: No effect on subcellular location. 1 Publication
    Mutagenesisi266 – 2661S → A: No effect on subcellular location. 1 Publication
    Mutagenesisi274 – 2741S → A: Impairs translocation from the cytoplasm to the nucleus upon glucose stimulation. 1 Publication
    Mutagenesisi274 – 2741S → D: No effect on subcellular location. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 357357Neurogenic differentiation factor 1PRO_0000127384Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei162 – 1621PhosphoserineBy similarity
    Modified residuei259 – 2591PhosphoserineBy similarity
    Modified residuei266 – 2661PhosphoserineBy similarity
    Modified residuei274 – 2741Phosphoserine1 Publication
    Modified residuei336 – 3361Phosphoserine; by CaMK21 Publication

    Post-translational modificationi

    Phosphorylated by MAPK1; phosphorylation regulates heterodimerization and DNA-binding activities. Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells By similarity. Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-336 by CaMK2; which promotes dendritic growth.By similarity2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ64289.

    PTM databases

    PhosphoSiteiQ64289.

    Expressioni

    Inductioni

    Up-regulated by fentanyl. Down-regulated by miR-190.1 Publication

    Gene expression databases

    GenevestigatoriQ64289.

    Interactioni

    Subunit structurei

    Heterodimer with TCF3/E47; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element. Interacts (via helix-loop-helix motif domain) with EP300 (via C-terminus); the interaction is inhibited by NR0B2. Interacts with TCF3; the interaction is inhibited by ID2. Interacts with RREB1 By similarity. Efficient DNA-binding requires dimerization with another bHLH protein. Heterodimer with TCF3/E47.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ64289.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini101 – 15353bHLHPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi87 – 937Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi58 – 7720Glu-rich (acidic)Add
    BLAST
    Compositional biasi67 – 7610Poly-Glu
    Compositional biasi87 – 904Poly-Lys

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG287211.
    GeneTreeiENSGT00680000099860.
    HOGENOMiHOG000049256.
    HOVERGENiHBG000250.
    InParanoidiQ64289.
    KOiK08033.
    OMAiMNAQLNA.
    OrthoDBiEOG722J8S.
    PhylomeDBiQ64289.
    TreeFamiTF315153.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR022575. Neurogenic_DUF.
    IPR016637. TF_bHLH_NeuroD.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    PF12533. Neuro_bHLH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015618. bHLH_NeuroD. 1 hit.
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q64289-1 [UniParc]FASTAAdd to Basket

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    MTKSYSESGL MGEPQPQGPP SWTDECLSSQ DEEHEADKKE DELEAMNAEE    50
    DSLRNGGEEE DEDEDLEEEE EEEEEEDDQK PKRRGPKKKK MTKARLERFK 100
    LRRMKANARE RNRMHGLNAA LDNLRKVVPC YSKTQKLSKI ETLRLAKNYI 150
    WALSEILRSG KSPDLVSFVQ TLCKGLSQPT TNLVAGCLQL NPRTFLPEQN 200
    PDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS 250
    AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS TEFEKNYAFT 300
    MHYPAATLAG PQSHGSIFSS GAAAPRCEIP IDNIMSFDSH SHHERVMSAQ 350
    LNAIFHD 357
    Length:357
    Mass (Da):40,001
    Last modified:November 1, 1996 - v1
    Checksum:iF773637E64D3E99E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D82075 mRNA. Translation: BAA11536.1.
    D82074 mRNA. Translation: BAA11535.1.
    D82945 mRNA. Translation: BAA81821.1.
    AF107728 mRNA. Translation: AAD19609.1.
    BC092367 mRNA. Translation: AAH92367.1.
    BC094526 mRNA. Translation: AAH94526.1.
    U80603 mRNA. Translation: AAB38744.1.
    PIRiJC4703.
    RefSeqiNP_062091.1. NM_019218.2.
    UniGeneiRn.44289.

    Genome annotation databases

    EnsembliENSRNOT00000007662; ENSRNOP00000007662; ENSRNOG00000005609.
    GeneIDi29458.
    KEGGirno:29458.
    UCSCiRGD:3165. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D82075 mRNA. Translation: BAA11536.1 .
    D82074 mRNA. Translation: BAA11535.1 .
    D82945 mRNA. Translation: BAA81821.1 .
    AF107728 mRNA. Translation: AAD19609.1 .
    BC092367 mRNA. Translation: AAH92367.1 .
    BC094526 mRNA. Translation: AAH94526.1 .
    U80603 mRNA. Translation: AAB38744.1 .
    PIRi JC4703.
    RefSeqi NP_062091.1. NM_019218.2.
    UniGenei Rn.44289.

    3D structure databases

    ProteinModelPortali Q64289.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q64289.

    Proteomic databases

    PaxDbi Q64289.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000007662 ; ENSRNOP00000007662 ; ENSRNOG00000005609 .
    GeneIDi 29458.
    KEGGi rno:29458.
    UCSCi RGD:3165. rat.

    Organism-specific databases

    CTDi 4760.
    RGDi 3165. Neurod1.

    Phylogenomic databases

    eggNOGi NOG287211.
    GeneTreei ENSGT00680000099860.
    HOGENOMi HOG000049256.
    HOVERGENi HBG000250.
    InParanoidi Q64289.
    KOi K08033.
    OMAi MNAQLNA.
    OrthoDBi EOG722J8S.
    PhylomeDBi Q64289.
    TreeFami TF315153.

    Miscellaneous databases

    NextBioi 609242.
    PROi Q64289.

    Gene expression databases

    Genevestigatori Q64289.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR022575. Neurogenic_DUF.
    IPR016637. TF_bHLH_NeuroD.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    PF12533. Neuro_bHLH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015618. bHLH_NeuroD. 1 hit.
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Cerebellum.
    2. "Overexpression of NeuroD in PC12 cells alters morphology and enhances expression of the adenylate kinase isozyme 1 gene."
      Noma T., Yoon Y.S., Nakazawa A.
      Brain Res. Mol. Brain Res. 67:53-63(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. "Cloning of rat NeuroD/Beta2."
      Andersen F.G., Madsen O.D., Serup P.
      Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: New England Deaconess Hospital.
      Tissue: Pancreatic islet.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "Developmental expression of neurogenic gene, NeuroD in the mammalian retina."
      Ahmad I., Acharay H.R.
      Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 88-200.
      Strain: Sprague-Dawley.
      Tissue: Retina.
    6. "Glucose induced MAPK signalling influences NeuroD1-mediated activation and nuclear localization."
      Petersen H.V., Jensen J.N., Stein R., Serup P.
      FEBS Lett. 528:241-245(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF SER-259; THR-262; SER-266 AND SER-274, PHOSPHORYLATION AT SER-274.
    7. "A CaMKII-NeuroD signaling pathway specifies dendritic morphogenesis."
      Gaudilliere B., Konishi Y., de la Iglesia N., Yao G., Bonni A.
      Neuron 41:229-241(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION AT SER-336.
    8. "Modulations of NeuroD activity contribute to the differential effects of morphine and fentanyl on dendritic spine stability."
      Zheng H., Zeng Y., Chu J., Kam A.Y., Loh H.H., Law P.Y.
      J. Neurosci. 30:8102-8110(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiNDF1_RAT
    AccessioniPrimary (citable) accession number: Q64289
    Secondary accession number(s): Q569P0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3