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Q64289

- NDF1_RAT

UniProt

Q64289 - NDF1_RAT

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Protein
Neurogenic differentiation factor 1
Gene
Neurod1, Neurod
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis By similarity.1 Publication

GO - Molecular functioni

  1. E-box binding Source: Ensembl
  2. RNA polymerase II transcription coactivator activity Source: Ensembl
  3. chromatin binding Source: UniProtKB
  4. double-stranded DNA binding Source: RGD
  5. sequence-specific DNA binding Source: Ensembl
  6. sequence-specific DNA binding transcription factor activity Source: Ensembl

GO - Biological processi

  1. amacrine cell differentiation Source: UniProtKB
  2. anterior/posterior pattern specification Source: Ensembl
  3. cellular response to glucose stimulus Source: UniProtKB
  4. cerebellum development Source: RGD
  5. dentate gyrus development Source: UniProtKB
  6. embryonic organ morphogenesis Source: Ensembl
  7. endocrine pancreas development Source: UniProtKB
  8. enteroendocrine cell differentiation Source: UniProtKB
  9. inner ear development Source: UniProtKB
  10. insulin secretion Source: Ensembl
  11. negative regulation of JAK-STAT cascade Source: Ensembl
  12. negative regulation of type B pancreatic cell apoptotic process Source: Ensembl
  13. nitric oxide mediated signal transduction Source: Ensembl
  14. nucleocytoplasmic transport Source: UniProtKB
  15. pancreatic A cell fate commitment Source: Ensembl
  16. pancreatic PP cell fate commitment Source: Ensembl
  17. positive regulation of apoptotic process Source: UniProtKB
  18. positive regulation of cell differentiation Source: UniProtKB
  19. positive regulation of neuron differentiation Source: RGD
  20. positive regulation of sequence-specific DNA binding transcription factor activity Source: Ensembl
  21. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  22. positive regulation of transcription regulatory region DNA binding Source: Ensembl
  23. positive regulation of transcription, DNA-templated Source: UniProtKB
  24. regulation of cell cycle arrest Source: UniProtKB
  25. regulation of intestinal epithelial structure maintenance Source: UniProtKB
  26. response to drug Source: RGD
  27. signal transduction involved in regulation of gene expression Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neurogenic differentiation factor 1
Short name:
NeuroD1
Alternative name(s):
Basic helix-loop-helix factor 1
Short name:
BHF-1
Gene namesi
Name:Neurod1
Synonyms:Neurod
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi3165. Neurod1.

Subcellular locationi

Cytoplasm. Nucleus
Note: Colocalizes with NR0B2 in the nucleus By similarity. In pancreatic islet cells, shuttles to the nucleus in response to glucose stimulation.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi259 – 2591S → A: No effect on subcellular location. 1 Publication
Mutagenesisi262 – 2621T → A: No effect on subcellular location. 1 Publication
Mutagenesisi266 – 2661S → A: No effect on subcellular location. 1 Publication
Mutagenesisi274 – 2741S → A: Impairs translocation from the cytoplasm to the nucleus upon glucose stimulation. 1 Publication
Mutagenesisi274 – 2741S → D: No effect on subcellular location. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Neurogenic differentiation factor 1
PRO_0000127384Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei162 – 1621Phosphoserine By similarity
Modified residuei259 – 2591Phosphoserine By similarity
Modified residuei266 – 2661Phosphoserine By similarity
Modified residuei274 – 2741Phosphoserine Inferred
Modified residuei336 – 3361Phosphoserine; by CaMK21 Publication

Post-translational modificationi

Phosphorylated by MAPK1; phosphorylation regulates heterodimerization and DNA-binding activities. Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells By similarity. Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-336 by CaMK2; which promotes dendritic growth.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64289.

PTM databases

PhosphoSiteiQ64289.

Expressioni

Inductioni

Up-regulated by fentanyl. Down-regulated by miR-190.1 Publication

Gene expression databases

GenevestigatoriQ64289.

Interactioni

Subunit structurei

Heterodimer with TCF3/E47; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element. Interacts (via helix-loop-helix motif domain) with EP300 (via C-terminus); the interaction is inhibited by NR0B2. Interacts with TCF3; the interaction is inhibited by ID2. Interacts with RREB1 By similarity. Efficient DNA-binding requires dimerization with another bHLH protein. Heterodimer with TCF3/E47.

Structurei

3D structure databases

ProteinModelPortaliQ64289.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 15353bHLH
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi87 – 937Nuclear localization signal Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi58 – 7720Glu-rich (acidic)
Add
BLAST
Compositional biasi67 – 7610Poly-Glu
Compositional biasi87 – 904Poly-Lys

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG287211.
GeneTreeiENSGT00680000099860.
HOGENOMiHOG000049256.
HOVERGENiHBG000250.
InParanoidiQ64289.
KOiK08033.
OMAiMNAQLNA.
OrthoDBiEOG722J8S.
PhylomeDBiQ64289.
TreeFamiTF315153.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR022575. Neurogenic_DUF.
IPR016637. TF_bHLH_NeuroD.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
PF12533. Neuro_bHLH. 1 hit.
[Graphical view]
PIRSFiPIRSF015618. bHLH_NeuroD. 1 hit.
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64289-1 [UniParc]FASTAAdd to Basket

« Hide

MTKSYSESGL MGEPQPQGPP SWTDECLSSQ DEEHEADKKE DELEAMNAEE    50
DSLRNGGEEE DEDEDLEEEE EEEEEEDDQK PKRRGPKKKK MTKARLERFK 100
LRRMKANARE RNRMHGLNAA LDNLRKVVPC YSKTQKLSKI ETLRLAKNYI 150
WALSEILRSG KSPDLVSFVQ TLCKGLSQPT TNLVAGCLQL NPRTFLPEQN 200
PDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS 250
AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS TEFEKNYAFT 300
MHYPAATLAG PQSHGSIFSS GAAAPRCEIP IDNIMSFDSH SHHERVMSAQ 350
LNAIFHD 357
Length:357
Mass (Da):40,001
Last modified:November 1, 1996 - v1
Checksum:iF773637E64D3E99E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D82075 mRNA. Translation: BAA11536.1.
D82074 mRNA. Translation: BAA11535.1.
D82945 mRNA. Translation: BAA81821.1.
AF107728 mRNA. Translation: AAD19609.1.
BC092367 mRNA. Translation: AAH92367.1.
BC094526 mRNA. Translation: AAH94526.1.
U80603 mRNA. Translation: AAB38744.1.
PIRiJC4703.
RefSeqiNP_062091.1. NM_019218.2.
UniGeneiRn.44289.

Genome annotation databases

EnsembliENSRNOT00000007662; ENSRNOP00000007662; ENSRNOG00000005609.
GeneIDi29458.
KEGGirno:29458.
UCSCiRGD:3165. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D82075 mRNA. Translation: BAA11536.1 .
D82074 mRNA. Translation: BAA11535.1 .
D82945 mRNA. Translation: BAA81821.1 .
AF107728 mRNA. Translation: AAD19609.1 .
BC092367 mRNA. Translation: AAH92367.1 .
BC094526 mRNA. Translation: AAH94526.1 .
U80603 mRNA. Translation: AAB38744.1 .
PIRi JC4703.
RefSeqi NP_062091.1. NM_019218.2.
UniGenei Rn.44289.

3D structure databases

ProteinModelPortali Q64289.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q64289.

Proteomic databases

PaxDbi Q64289.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000007662 ; ENSRNOP00000007662 ; ENSRNOG00000005609 .
GeneIDi 29458.
KEGGi rno:29458.
UCSCi RGD:3165. rat.

Organism-specific databases

CTDi 4760.
RGDi 3165. Neurod1.

Phylogenomic databases

eggNOGi NOG287211.
GeneTreei ENSGT00680000099860.
HOGENOMi HOG000049256.
HOVERGENi HBG000250.
InParanoidi Q64289.
KOi K08033.
OMAi MNAQLNA.
OrthoDBi EOG722J8S.
PhylomeDBi Q64289.
TreeFami TF315153.

Miscellaneous databases

NextBioi 609242.
PROi Q64289.

Gene expression databases

Genevestigatori Q64289.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
IPR022575. Neurogenic_DUF.
IPR016637. TF_bHLH_NeuroD.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
PF12533. Neuro_bHLH. 1 hit.
[Graphical view ]
PIRSFi PIRSF015618. bHLH_NeuroD. 1 hit.
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Cerebellum.
  2. "Overexpression of NeuroD in PC12 cells alters morphology and enhances expression of the adenylate kinase isozyme 1 gene."
    Noma T., Yoon Y.S., Nakazawa A.
    Brain Res. Mol. Brain Res. 67:53-63(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "Cloning of rat NeuroD/Beta2."
    Andersen F.G., Madsen O.D., Serup P.
    Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: New England Deaconess Hospital.
    Tissue: Pancreatic islet.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Developmental expression of neurogenic gene, NeuroD in the mammalian retina."
    Ahmad I., Acharay H.R.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 88-200.
    Strain: Sprague-Dawley.
    Tissue: Retina.
  6. "Glucose induced MAPK signalling influences NeuroD1-mediated activation and nuclear localization."
    Petersen H.V., Jensen J.N., Stein R., Serup P.
    FEBS Lett. 528:241-245(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF SER-259; THR-262; SER-266 AND SER-274, PHOSPHORYLATION AT SER-274.
  7. "A CaMKII-NeuroD signaling pathway specifies dendritic morphogenesis."
    Gaudilliere B., Konishi Y., de la Iglesia N., Yao G., Bonni A.
    Neuron 41:229-241(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT SER-336.
  8. "Modulations of NeuroD activity contribute to the differential effects of morphine and fentanyl on dendritic spine stability."
    Zheng H., Zeng Y., Chu J., Kam A.Y., Loh H.H., Law P.Y.
    J. Neurosci. 30:8102-8110(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiNDF1_RAT
AccessioniPrimary (citable) accession number: Q64289
Secondary accession number(s): Q569P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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