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Protein

Interferon regulatory factor 4

Gene

Irf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi21 – 129IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd BLAST109

GO - Molecular functioni

GO - Biological processi

  • defense response to protozoan Source: UniProtKB
  • histone H3 acetylation Source: MGI
  • histone H4 acetylation Source: MGI
  • myeloid dendritic cell differentiation Source: UniProtKB
  • negative regulation of toll-like receptor signaling pathway Source: MGI
  • positive regulation of DNA binding Source: MGI
  • positive regulation of interleukin-10 biosynthetic process Source: UniProtKB
  • positive regulation of interleukin-13 biosynthetic process Source: UniProtKB
  • positive regulation of interleukin-2 biosynthetic process Source: UniProtKB
  • positive regulation of interleukin-4 biosynthetic process Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein methylation Source: UniProtKB
  • T-helper 17 cell lineage commitment Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-877300. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 4
Short name:
IRF-4
Alternative name(s):
Lymphocyte-specific interferon regulatory factor
Short name:
LSIRF
NF-EM5
PU.1 interaction partner
Transcriptional activator PIP
Gene namesi
Name:Irf4
Synonyms:Spip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1096873. Irf4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • membrane Source: MGI
  • nuclear nucleosome Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001545571 – 450Interferon regulatory factor 4Add BLAST450

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei446Phosphoserine; by ROCK21 Publication1
Modified residuei447Phosphoserine; by ROCK21 Publication1

Post-translational modificationi

Phosphorylation by ROCK2 regulates IL-17 and IL-21 production.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64287.
PRIDEiQ64287.

PTM databases

iPTMnetiQ64287.
PhosphoSitePlusiQ64287.

Expressioni

Tissue specificityi

Lymphoid cells.

Inductioni

Not induced by interferons.

Gene expression databases

BgeeiENSMUSG00000021356.
CleanExiMM_IRF4.
ExpressionAtlasiQ64287. baseline and differential.
GenevisibleiQ64287. MM.

Interactioni

Subunit structurei

Interacts with SPIB and DEF6 (By similarity). Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct. Directly interacts with NLRP3 in the nucleus of Th2 cells; this interaction enhances IRF4 ability to bind to the IL4 promoter and is required for optimal IRF4-dependent IL4 transcription (PubMed:26098997).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BatfO352847EBI-6398485,EBI-6398523
Ppargc1aO703436EBI-6398485,EBI-1371053

Protein-protein interaction databases

BioGridi200786. 7 interactors.
DIPiDIP-59740N.
IntActiQ64287. 4 interactors.
STRINGi10090.ENSMUSP00000021784.

Structurei

Secondary structure

1450
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi241 – 248Combined sources8
Beta strandi252 – 258Combined sources7
Beta strandi261 – 267Combined sources7
Beta strandi274 – 279Combined sources6
Beta strandi286 – 291Combined sources6
Beta strandi297 – 300Combined sources4
Helixi301 – 308Combined sources8
Beta strandi315 – 319Combined sources5
Beta strandi321 – 328Combined sources8
Beta strandi330 – 332Combined sources3
Beta strandi334 – 338Combined sources5
Beta strandi343 – 345Combined sources3
Beta strandi355 – 360Combined sources6
Helixi361 – 374Combined sources14
Beta strandi383 – 388Combined sources6
Beta strandi400 – 407Combined sources8
Helixi408 – 418Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5BVIX-ray2.60A/B238-420[»]
ProteinModelPortaliQ64287.
SMRiQ64287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IRF family.PROSITE-ProRule annotation
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IES5. Eukaryota.
ENOG411023V. LUCA.
GeneTreeiENSGT00760000119093.
HOGENOMiHOG000010107.
HOVERGENiHBG003072.
InParanoidiQ64287.
KOiK09445.
OMAiDNGQRKN.
OrthoDBiEOG091G067P.
PhylomeDBiQ64287.
TreeFamiTF328512.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
SM01243. IRF-3. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF49879. SSF49879. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q64287-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNLETGSRGS EFGMSAVSCG NGKLRQWLID QIDSGKYPGL VWENEEKSVF
60 70 80 90 100
RIPWKHAGKQ DYNREEDAAL FKAWALFKGK FREGIDKPDP PTWKTRLRCA
110 120 130 140 150
LNKSNDFEEL VERSQLDISD PYKVYRIVPE GAKKGAKQLT LDDTQMAMGH
160 170 180 190 200
PYPMTAPYGS LPAQQVHNYM MPPHDRSWRD YAPDQSHPEI PYQCPVTFGP
210 220 230 240 250
RGHHWQGPSC ENGCQVTGTF YACAPPESQA PGIPIEPSIR SAEALALSDC
260 270 280 290 300
RLHICLYYRD ILVKELTTTS PEGCRISHGH TYDVSNLDQV LFPYPDDNGQ
310 320 330 340 350
RKNIEKLLSH LERGLVLWMA PDGLYAKRLC QSRIYWDGPL ALCSDRPNKL
360 370 380 390 400
ERDQTCKLFD TQQFLSELQV FAHHGRPAPR FQVTLCFGEE FPDPQRQRKL
410 420 430 440 450
ITAHVEPLLA RQLYYFAQQN TGHFLRGYEL PEHVTTPDYH RSLRHSSIQE
Length:450
Mass (Da):51,577
Last modified:November 1, 1997 - v1
Checksum:i5FD94CA6C453869C
GO
Isoform 2 (identifier: Q64287-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     165-165: Missing.

Show »
Length:449
Mass (Da):51,449
Checksum:iC4E4A0DC04AA8A52
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002756165Missing in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34307 mRNA. Translation: AAA75283.1.
U11692 mRNA. Translation: AAA75309.1.
U20949 Genomic DNA. Translation: AAA75316.1.
U20949 Genomic DNA. Translation: AAA75317.1.
CCDSiCCDS26419.1. [Q64287-1]
PIRiS57837.
RefSeqiNP_038702.1. NM_013674.1. [Q64287-1]
XP_006516623.1. XM_006516560.1. [Q64287-2]
UniGeneiMm.4677.

Genome annotation databases

EnsembliENSMUST00000021784; ENSMUSP00000021784; ENSMUSG00000021356. [Q64287-1]
ENSMUST00000110307; ENSMUSP00000105936; ENSMUSG00000021356. [Q64287-2]
GeneIDi16364.
KEGGimmu:16364.
UCSCiuc007pyz.1. mouse. [Q64287-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34307 mRNA. Translation: AAA75283.1.
U11692 mRNA. Translation: AAA75309.1.
U20949 Genomic DNA. Translation: AAA75316.1.
U20949 Genomic DNA. Translation: AAA75317.1.
CCDSiCCDS26419.1. [Q64287-1]
PIRiS57837.
RefSeqiNP_038702.1. NM_013674.1. [Q64287-1]
XP_006516623.1. XM_006516560.1. [Q64287-2]
UniGeneiMm.4677.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5BVIX-ray2.60A/B238-420[»]
ProteinModelPortaliQ64287.
SMRiQ64287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200786. 7 interactors.
DIPiDIP-59740N.
IntActiQ64287. 4 interactors.
STRINGi10090.ENSMUSP00000021784.

PTM databases

iPTMnetiQ64287.
PhosphoSitePlusiQ64287.

Proteomic databases

PaxDbiQ64287.
PRIDEiQ64287.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021784; ENSMUSP00000021784; ENSMUSG00000021356. [Q64287-1]
ENSMUST00000110307; ENSMUSP00000105936; ENSMUSG00000021356. [Q64287-2]
GeneIDi16364.
KEGGimmu:16364.
UCSCiuc007pyz.1. mouse. [Q64287-1]

Organism-specific databases

CTDi3662.
MGIiMGI:1096873. Irf4.

Phylogenomic databases

eggNOGiENOG410IES5. Eukaryota.
ENOG411023V. LUCA.
GeneTreeiENSGT00760000119093.
HOGENOMiHOG000010107.
HOVERGENiHBG003072.
InParanoidiQ64287.
KOiK09445.
OMAiDNGQRKN.
OrthoDBiEOG091G067P.
PhylomeDBiQ64287.
TreeFamiTF328512.

Enzyme and pathway databases

ReactomeiR-MMU-877300. Interferon gamma signaling.

Miscellaneous databases

PROiQ64287.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021356.
CleanExiMM_IRF4.
ExpressionAtlasiQ64287. baseline and differential.
GenevisibleiQ64287. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
SM01243. IRF-3. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF49879. SSF49879. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIRF4_MOUSE
AccessioniPrimary (citable) accession number: Q64287
Secondary accession number(s): Q60802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.