UniProtKB - Q64287 (IRF4_MOUSE)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
Interferon regulatory factor 4
Gene
Irf4
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes.3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 21 – 129 | IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd BLAST | 109 |
GO - Molecular functioni
- DNA binding transcription factor activity Source: UniProtKB
- protein-lysine N-methyltransferase activity Source: UniProtKB
- RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: UniProtKB
- transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
GO - Biological processi
- defense response to protozoan Source: UniProtKB
- histone H3 acetylation Source: MGI
- histone H4 acetylation Source: MGI
- myeloid dendritic cell differentiation Source: UniProtKB
- negative regulation of toll-like receptor signaling pathway Source: MGI
- positive regulation of DNA binding Source: MGI
- positive regulation of interleukin-10 biosynthetic process Source: UniProtKB
- positive regulation of interleukin-13 biosynthetic process Source: UniProtKB
- positive regulation of interleukin-2 biosynthetic process Source: UniProtKB
- positive regulation of interleukin-4 biosynthetic process Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- protein methylation Source: UniProtKB
- T-helper 17 cell lineage commitment Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Interferon regulatory factor 4Short name: IRF-4 Alternative name(s): Lymphocyte-specific interferon regulatory factor Short name: LSIRF NF-EM5 PU.1 interaction partner Transcriptional activator PIP |
Gene namesi | Name:Irf4 Synonyms:Spip |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096873. Irf4. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154557 | 1 – 450 | Interferon regulatory factor 4Add BLAST | 450 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 446 | Phosphoserine; by ROCK21 Publication | 1 | |
Modified residuei | 447 | Phosphoserine; by ROCK21 Publication | 1 |
Post-translational modificationi
Phosphorylation by ROCK2 regulates IL-17 and IL-21 production.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q64287. |
PRIDEi | Q64287. |
PTM databases
iPTMneti | Q64287. |
PhosphoSitePlusi | Q64287. |
Expressioni
Tissue specificityi
Lymphoid cells.
Inductioni
Not induced by interferons.
Gene expression databases
Bgeei | ENSMUSG00000021356. |
CleanExi | MM_IRF4. |
ExpressionAtlasi | Q64287. baseline and differential. |
Genevisiblei | Q64287. MM. |
Interactioni
Subunit structurei
Interacts with SPIB and DEF6 (By similarity). Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct. Directly interacts with NLRP3 in the nucleus of Th2 cells; this interaction enhances IRF4 ability to bind to the IL4 promoter and is required for optimal IRF4-dependent IL4 transcription (PubMed:26098997).By similarity4 Publications
Binary interactionsi
Protein-protein interaction databases
BioGridi | 200786. 7 interactors. |
DIPi | DIP-59740N. |
IntActi | Q64287. 5 interactors. |
STRINGi | 10090.ENSMUSP00000021784. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 241 – 248 | Combined sources | 8 | |
Beta strandi | 252 – 258 | Combined sources | 7 | |
Beta strandi | 261 – 267 | Combined sources | 7 | |
Beta strandi | 274 – 279 | Combined sources | 6 | |
Beta strandi | 286 – 291 | Combined sources | 6 | |
Beta strandi | 297 – 300 | Combined sources | 4 | |
Helixi | 301 – 308 | Combined sources | 8 | |
Beta strandi | 315 – 319 | Combined sources | 5 | |
Beta strandi | 321 – 328 | Combined sources | 8 | |
Beta strandi | 330 – 332 | Combined sources | 3 | |
Beta strandi | 334 – 338 | Combined sources | 5 | |
Beta strandi | 343 – 345 | Combined sources | 3 | |
Beta strandi | 355 – 360 | Combined sources | 6 | |
Helixi | 361 – 374 | Combined sources | 14 | |
Beta strandi | 383 – 388 | Combined sources | 6 | |
Beta strandi | 400 – 407 | Combined sources | 8 | |
Helixi | 408 – 418 | Combined sources | 11 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5BVI | X-ray | 2.60 | A/B | 238-420 | [»] | |
ProteinModelPortali | Q64287. | |||||
SMRi | Q64287. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the IRF family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | ENOG410IES5. Eukaryota. ENOG411023V. LUCA. |
GeneTreei | ENSGT00760000119093. |
HOGENOMi | HOG000010107. |
HOVERGENi | HBG003072. |
InParanoidi | Q64287. |
KOi | K09445. |
OMAi | DCRLHIC. |
OrthoDBi | EOG091G067P. |
PhylomeDBi | Q64287. |
TreeFami | TF328512. |
Family and domain databases
CDDi | cd00103. IRF. 1 hit. |
Gene3Di | 1.10.10.10. 1 hit. 2.60.200.10. 1 hit. |
InterProi | View protein in InterPro IPR019817. Interferon_reg_fac_CS. IPR001346. Interferon_reg_fact_DNA-bd_dom. IPR019471. Interferon_reg_factor-3. IPR017855. SMAD-like_dom_sf. IPR008984. SMAD_FHA_dom_sf. IPR036388. WH-like_DNA-bd_sf. IPR036390. WH_DNA-bd_sf. |
PANTHERi | PTHR11949. PTHR11949. 1 hit. |
Pfami | View protein in Pfam PF00605. IRF. 1 hit. PF10401. IRF-3. 1 hit. |
PRINTSi | PR00267. INTFRNREGFCT. |
SMARTi | View protein in SMART SM00348. IRF. 1 hit. SM01243. IRF-3. 1 hit. |
SUPFAMi | SSF46785. SSF46785. 1 hit. SSF49879. SSF49879. 1 hit. |
PROSITEi | View protein in PROSITE PS00601. IRF_1. 1 hit. PS51507. IRF_2. 1 hit. |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q64287-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MNLETGSRGS EFGMSAVSCG NGKLRQWLID QIDSGKYPGL VWENEEKSVF
60 70 80 90 100
RIPWKHAGKQ DYNREEDAAL FKAWALFKGK FREGIDKPDP PTWKTRLRCA
110 120 130 140 150
LNKSNDFEEL VERSQLDISD PYKVYRIVPE GAKKGAKQLT LDDTQMAMGH
160 170 180 190 200
PYPMTAPYGS LPAQQVHNYM MPPHDRSWRD YAPDQSHPEI PYQCPVTFGP
210 220 230 240 250
RGHHWQGPSC ENGCQVTGTF YACAPPESQA PGIPIEPSIR SAEALALSDC
260 270 280 290 300
RLHICLYYRD ILVKELTTTS PEGCRISHGH TYDVSNLDQV LFPYPDDNGQ
310 320 330 340 350
RKNIEKLLSH LERGLVLWMA PDGLYAKRLC QSRIYWDGPL ALCSDRPNKL
360 370 380 390 400
ERDQTCKLFD TQQFLSELQV FAHHGRPAPR FQVTLCFGEE FPDPQRQRKL
410 420 430 440 450
ITAHVEPLLA RQLYYFAQQN TGHFLRGYEL PEHVTTPDYH RSLRHSSIQE
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002756 | 165 | Missing in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U34307 mRNA. Translation: AAA75283.1. U11692 mRNA. Translation: AAA75309.1. U20949 Genomic DNA. Translation: AAA75316.1. U20949 Genomic DNA. Translation: AAA75317.1. |
CCDSi | CCDS26419.1. [Q64287-1] CCDS84017.1. [Q64287-2] |
PIRi | S57837. |
RefSeqi | NP_001334437.1. NM_001347508.1. [Q64287-2] NP_038702.1. NM_013674.2. [Q64287-1] |
UniGenei | Mm.4677. |
Genome annotation databases
Ensembli | ENSMUST00000021784; ENSMUSP00000021784; ENSMUSG00000021356. [Q64287-1] ENSMUST00000110307; ENSMUSP00000105936; ENSMUSG00000021356. [Q64287-2] |
GeneIDi | 16364. |
KEGGi | mmu:16364. |
UCSCi | uc007pyz.1. mouse. [Q64287-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | IRF4_MOUSE | |
Accessioni | Q64287Primary (citable) accession number: Q64287 Secondary accession number(s): Q60802 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | March 28, 2018 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |