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Q64287

- IRF4_MOUSE

UniProt

Q64287 - IRF4_MOUSE

Protein

Interferon regulatory factor 4

Gene

Irf4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi21 – 129109IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. protein-lysine N-methyltransferase activity Source: UniProtKB
    3. regulatory region DNA binding Source: InterPro
    4. sequence-specific DNA binding Source: UniProtKB
    5. sequence-specific DNA binding transcription factor activity Source: UniProtKB

    GO - Biological processi

    1. defense response to protozoan Source: UniProtKB
    2. histone H3 acetylation Source: MGI
    3. histone H4 acetylation Source: MGI
    4. myeloid dendritic cell differentiation Source: UniProtKB
    5. negative regulation of toll-like receptor signaling pathway Source: MGI
    6. peptidyl-lysine methylation Source: GOC
    7. positive regulation of DNA binding Source: MGI
    8. positive regulation of interleukin-10 biosynthetic process Source: UniProtKB
    9. positive regulation of interleukin-13 biosynthetic process Source: UniProtKB
    10. positive regulation of interleukin-2 biosynthetic process Source: UniProtKB
    11. positive regulation of interleukin-4 biosynthetic process Source: UniProtKB
    12. positive regulation of transcription, DNA-templated Source: UniProtKB
    13. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    14. protein methylation Source: UniProtKB
    15. T-helper 17 cell lineage commitment Source: UniProtKB
    16. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198660. Interferon gamma signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interferon regulatory factor 4
    Short name:
    IRF-4
    Alternative name(s):
    Lymphocyte-specific interferon regulatory factor
    Short name:
    LSIRF
    NF-EM5
    PU.1 interaction partner
    Transcriptional activator PIP
    Gene namesi
    Name:Irf4
    Synonyms:Spip
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1096873. Irf4.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nuclear nucleosome Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 450450Interferon regulatory factor 4PRO_0000154557Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei446 – 4461Phosphoserine; by ROCK21 Publication
    Modified residuei447 – 4471Phosphoserine; by ROCK21 Publication

    Post-translational modificationi

    Phosphorylation by ROCK2 regulates IL-17 and IL-21 production.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ64287.

    PTM databases

    PhosphoSiteiQ64287.

    Expressioni

    Tissue specificityi

    Lymphoid cells.

    Inductioni

    Not induced by interferons.

    Gene expression databases

    ArrayExpressiQ64287.
    BgeeiQ64287.
    CleanExiMM_IRF4.
    GenevestigatoriQ64287.

    Interactioni

    Subunit structurei

    Interacts with SPIB and DEF6 By similarity. Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BatfO352847EBI-6398485,EBI-6398523

    Protein-protein interaction databases

    BioGridi200786. 4 interactions.
    DIPiDIP-59740N.
    IntActiQ64287. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64287.
    SMRiQ64287. Positions 18-130, 252-418.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the IRF family.PROSITE-ProRule annotation
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG39558.
    GeneTreeiENSGT00740000114956.
    HOGENOMiHOG000010107.
    HOVERGENiHBG003072.
    InParanoidiQ64287.
    KOiK09445.
    OMAiDNGQRKN.
    OrthoDBiEOG7CCBR1.
    PhylomeDBiQ64287.
    TreeFamiTF328512.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.200.10. 1 hit.
    InterProiIPR019817. Interferon_reg_fac_CS.
    IPR001346. Interferon_reg_fact_DNA-bd_dom.
    IPR019471. Interferon_reg_factor-3.
    IPR017855. SMAD_dom-like.
    IPR008984. SMAD_FHA_domain.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00605. IRF. 1 hit.
    PF10401. IRF-3. 1 hit.
    [Graphical view]
    PRINTSiPR00267. INTFRNREGFCT.
    SMARTiSM00348. IRF. 1 hit.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.
    PROSITEiPS00601. IRF_1. 1 hit.
    PS51507. IRF_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q64287-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNLETGSRGS EFGMSAVSCG NGKLRQWLID QIDSGKYPGL VWENEEKSVF    50
    RIPWKHAGKQ DYNREEDAAL FKAWALFKGK FREGIDKPDP PTWKTRLRCA 100
    LNKSNDFEEL VERSQLDISD PYKVYRIVPE GAKKGAKQLT LDDTQMAMGH 150
    PYPMTAPYGS LPAQQVHNYM MPPHDRSWRD YAPDQSHPEI PYQCPVTFGP 200
    RGHHWQGPSC ENGCQVTGTF YACAPPESQA PGIPIEPSIR SAEALALSDC 250
    RLHICLYYRD ILVKELTTTS PEGCRISHGH TYDVSNLDQV LFPYPDDNGQ 300
    RKNIEKLLSH LERGLVLWMA PDGLYAKRLC QSRIYWDGPL ALCSDRPNKL 350
    ERDQTCKLFD TQQFLSELQV FAHHGRPAPR FQVTLCFGEE FPDPQRQRKL 400
    ITAHVEPLLA RQLYYFAQQN TGHFLRGYEL PEHVTTPDYH RSLRHSSIQE 450
    Length:450
    Mass (Da):51,577
    Last modified:November 1, 1997 - v1
    Checksum:i5FD94CA6C453869C
    GO
    Isoform 2 (identifier: Q64287-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         165-165: Missing.

    Show »
    Length:449
    Mass (Da):51,449
    Checksum:iC4E4A0DC04AA8A52
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei165 – 1651Missing in isoform 2. CuratedVSP_002756

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34307 mRNA. Translation: AAA75283.1.
    U11692 mRNA. Translation: AAA75309.1.
    U20949 Genomic DNA. Translation: AAA75316.1.
    U20949 Genomic DNA. Translation: AAA75317.1.
    CCDSiCCDS26419.1. [Q64287-1]
    PIRiS57837.
    RefSeqiNP_038702.1. NM_013674.1. [Q64287-1]
    XP_006516623.1. XM_006516560.1. [Q64287-2]
    UniGeneiMm.4677.

    Genome annotation databases

    EnsembliENSMUST00000021784; ENSMUSP00000021784; ENSMUSG00000021356. [Q64287-1]
    ENSMUST00000110307; ENSMUSP00000105936; ENSMUSG00000021356. [Q64287-2]
    GeneIDi16364.
    KEGGimmu:16364.
    UCSCiuc007pyz.1. mouse. [Q64287-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34307 mRNA. Translation: AAA75283.1 .
    U11692 mRNA. Translation: AAA75309.1 .
    U20949 Genomic DNA. Translation: AAA75316.1 .
    U20949 Genomic DNA. Translation: AAA75317.1 .
    CCDSi CCDS26419.1. [Q64287-1 ]
    PIRi S57837.
    RefSeqi NP_038702.1. NM_013674.1. [Q64287-1 ]
    XP_006516623.1. XM_006516560.1. [Q64287-2 ]
    UniGenei Mm.4677.

    3D structure databases

    ProteinModelPortali Q64287.
    SMRi Q64287. Positions 18-130, 252-418.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200786. 4 interactions.
    DIPi DIP-59740N.
    IntActi Q64287. 1 interaction.

    PTM databases

    PhosphoSitei Q64287.

    Proteomic databases

    PRIDEi Q64287.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021784 ; ENSMUSP00000021784 ; ENSMUSG00000021356 . [Q64287-1 ]
    ENSMUST00000110307 ; ENSMUSP00000105936 ; ENSMUSG00000021356 . [Q64287-2 ]
    GeneIDi 16364.
    KEGGi mmu:16364.
    UCSCi uc007pyz.1. mouse. [Q64287-1 ]

    Organism-specific databases

    CTDi 3662.
    MGIi MGI:1096873. Irf4.

    Phylogenomic databases

    eggNOGi NOG39558.
    GeneTreei ENSGT00740000114956.
    HOGENOMi HOG000010107.
    HOVERGENi HBG003072.
    InParanoidi Q64287.
    KOi K09445.
    OMAi DNGQRKN.
    OrthoDBi EOG7CCBR1.
    PhylomeDBi Q64287.
    TreeFami TF328512.

    Enzyme and pathway databases

    Reactomei REACT_198660. Interferon gamma signaling.

    Miscellaneous databases

    NextBioi 289474.
    PROi Q64287.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64287.
    Bgeei Q64287.
    CleanExi MM_IRF4.
    Genevestigatori Q64287.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.200.10. 1 hit.
    InterProi IPR019817. Interferon_reg_fac_CS.
    IPR001346. Interferon_reg_fact_DNA-bd_dom.
    IPR019471. Interferon_reg_factor-3.
    IPR017855. SMAD_dom-like.
    IPR008984. SMAD_FHA_domain.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00605. IRF. 1 hit.
    PF10401. IRF-3. 1 hit.
    [Graphical view ]
    PRINTSi PR00267. INTFRNREGFCT.
    SMARTi SM00348. IRF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    PROSITEi PS00601. IRF_1. 1 hit.
    PS51507. IRF_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Pip, a novel IRF family member, is a lymphoid-specific, PU.1-dependent transcriptional activator."
      Eisenbeis C.F., Singh H., Storb U.
      Genes Dev. 9:1377-1387(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "Molecular cloning of LSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE)."
      Matsuyama T., Grossman A., Mittruecker H.-W., Siderovski D.P., Kiefer F., Kawakami T., Richardson C.D., Taniguchi T., Yoshinaga S.K., Mak T.W.
      Nucleic Acids Res. 23:2127-2136(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129/SvJ and C57BL/6.
      Tissue: Spleen.
    3. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 65-72, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    4. "Phosphorylation of IRF4 by ROCK2 regulates IL-17 and IL-21 production and the development of autoimmunity in mice."
      Biswas P.S., Gupta S., Chang E., Song L., Stirzaker R.A., Liao J.K., Bhagat G., Pernis A.B.
      J. Clin. Invest. 120:3280-3295(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-446 AND SER-447.
    5. Cited for: FUNCTION, INTERACTION WITH BATF.
    6. "BATF-JUN is critical for IRF4-mediated transcription in T cells."
      Li P., Spolski R., Liao W., Wang L., Murphy T.L., Murphy K.M., Leonard W.J.
      Nature 490:543-546(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH BATF.
    7. Cited for: FUNCTION, INTERACTION WITH BATF.

    Entry informationi

    Entry nameiIRF4_MOUSE
    AccessioniPrimary (citable) accession number: Q64287
    Secondary accession number(s): Q60802
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3