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Q64287

- IRF4_MOUSE

UniProt

Q64287 - IRF4_MOUSE

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Protein
Interferon regulatory factor 4
Gene
Irf4, Spip
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi21 – 129109IRF tryptophan pentad repeat
Add
BLAST

GO - Molecular functioni

  1. protein binding Source: UniProtKB
  2. protein-lysine N-methyltransferase activity Source: UniProtKB
  3. regulatory region DNA binding Source: InterPro
  4. sequence-specific DNA binding Source: UniProtKB
  5. sequence-specific DNA binding transcription factor activity Source: UniProtKB

GO - Biological processi

  1. T-helper 17 cell lineage commitment Source: UniProtKB
  2. defense response to protozoan Source: UniProtKB
  3. histone H3 acetylation Source: MGI
  4. histone H4 acetylation Source: MGI
  5. myeloid dendritic cell differentiation Source: UniProtKB
  6. negative regulation of toll-like receptor signaling pathway Source: MGI
  7. peptidyl-lysine methylation Source: GOC
  8. positive regulation of DNA binding Source: MGI
  9. positive regulation of interleukin-10 biosynthetic process Source: UniProtKB
  10. positive regulation of interleukin-13 biosynthetic process Source: UniProtKB
  11. positive regulation of interleukin-2 biosynthetic process Source: UniProtKB
  12. positive regulation of interleukin-4 biosynthetic process Source: UniProtKB
  13. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  14. positive regulation of transcription, DNA-templated Source: UniProtKB
  15. protein methylation Source: UniProtKB
  16. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_198660. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 4
Short name:
IRF-4
Alternative name(s):
Lymphocyte-specific interferon regulatory factor
Short name:
LSIRF
NF-EM5
PU.1 interaction partner
Transcriptional activator PIP
Gene namesi
Name:Irf4
Synonyms:Spip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:1096873. Irf4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nuclear nucleosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450Interferon regulatory factor 4
PRO_0000154557Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei446 – 4461Phosphoserine; by ROCK21 Publication
Modified residuei447 – 4471Phosphoserine; by ROCK21 Publication

Post-translational modificationi

Phosphorylation by ROCK2 regulates IL-17 and IL-21 production.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ64287.

PTM databases

PhosphoSiteiQ64287.

Expressioni

Tissue specificityi

Lymphoid cells.

Inductioni

Not induced by interferons.

Gene expression databases

ArrayExpressiQ64287.
BgeeiQ64287.
CleanExiMM_IRF4.
GenevestigatoriQ64287.

Interactioni

Subunit structurei

Interacts with SPIB and DEF6 By similarity. Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BatfO352847EBI-6398485,EBI-6398523

Protein-protein interaction databases

BioGridi200786. 3 interactions.
DIPiDIP-59740N.
IntActiQ64287. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ64287.
SMRiQ64287. Positions 18-130, 252-418.

Family & Domainsi

Sequence similaritiesi

Belongs to the IRF family.

Phylogenomic databases

eggNOGiNOG39558.
GeneTreeiENSGT00740000114956.
HOGENOMiHOG000010107.
HOVERGENiHBG003072.
InParanoidiQ64287.
KOiK09445.
OMAiDNGQRKN.
OrthoDBiEOG7CCBR1.
PhylomeDBiQ64287.
TreeFamiTF328512.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q64287-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MNLETGSRGS EFGMSAVSCG NGKLRQWLID QIDSGKYPGL VWENEEKSVF    50
RIPWKHAGKQ DYNREEDAAL FKAWALFKGK FREGIDKPDP PTWKTRLRCA 100
LNKSNDFEEL VERSQLDISD PYKVYRIVPE GAKKGAKQLT LDDTQMAMGH 150
PYPMTAPYGS LPAQQVHNYM MPPHDRSWRD YAPDQSHPEI PYQCPVTFGP 200
RGHHWQGPSC ENGCQVTGTF YACAPPESQA PGIPIEPSIR SAEALALSDC 250
RLHICLYYRD ILVKELTTTS PEGCRISHGH TYDVSNLDQV LFPYPDDNGQ 300
RKNIEKLLSH LERGLVLWMA PDGLYAKRLC QSRIYWDGPL ALCSDRPNKL 350
ERDQTCKLFD TQQFLSELQV FAHHGRPAPR FQVTLCFGEE FPDPQRQRKL 400
ITAHVEPLLA RQLYYFAQQN TGHFLRGYEL PEHVTTPDYH RSLRHSSIQE 450
Length:450
Mass (Da):51,577
Last modified:November 1, 1997 - v1
Checksum:i5FD94CA6C453869C
GO
Isoform 2 (identifier: Q64287-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     165-165: Missing.

Show »
Length:449
Mass (Da):51,449
Checksum:iC4E4A0DC04AA8A52
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei165 – 1651Missing in isoform 2.
VSP_002756

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U34307 mRNA. Translation: AAA75283.1.
U11692 mRNA. Translation: AAA75309.1.
U20949 Genomic DNA. Translation: AAA75316.1.
U20949 Genomic DNA. Translation: AAA75317.1.
CCDSiCCDS26419.1. [Q64287-1]
PIRiS57837.
RefSeqiNP_038702.1. NM_013674.1. [Q64287-1]
XP_006516623.1. XM_006516560.1. [Q64287-2]
UniGeneiMm.4677.

Genome annotation databases

EnsembliENSMUST00000021784; ENSMUSP00000021784; ENSMUSG00000021356. [Q64287-1]
ENSMUST00000110307; ENSMUSP00000105936; ENSMUSG00000021356. [Q64287-2]
GeneIDi16364.
KEGGimmu:16364.
UCSCiuc007pyz.1. mouse. [Q64287-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U34307 mRNA. Translation: AAA75283.1 .
U11692 mRNA. Translation: AAA75309.1 .
U20949 Genomic DNA. Translation: AAA75316.1 .
U20949 Genomic DNA. Translation: AAA75317.1 .
CCDSi CCDS26419.1. [Q64287-1 ]
PIRi S57837.
RefSeqi NP_038702.1. NM_013674.1. [Q64287-1 ]
XP_006516623.1. XM_006516560.1. [Q64287-2 ]
UniGenei Mm.4677.

3D structure databases

ProteinModelPortali Q64287.
SMRi Q64287. Positions 18-130, 252-418.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200786. 3 interactions.
DIPi DIP-59740N.
IntActi Q64287. 1 interaction.

PTM databases

PhosphoSitei Q64287.

Proteomic databases

PRIDEi Q64287.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021784 ; ENSMUSP00000021784 ; ENSMUSG00000021356 . [Q64287-1 ]
ENSMUST00000110307 ; ENSMUSP00000105936 ; ENSMUSG00000021356 . [Q64287-2 ]
GeneIDi 16364.
KEGGi mmu:16364.
UCSCi uc007pyz.1. mouse. [Q64287-1 ]

Organism-specific databases

CTDi 3662.
MGIi MGI:1096873. Irf4.

Phylogenomic databases

eggNOGi NOG39558.
GeneTreei ENSGT00740000114956.
HOGENOMi HOG000010107.
HOVERGENi HBG003072.
InParanoidi Q64287.
KOi K09445.
OMAi DNGQRKN.
OrthoDBi EOG7CCBR1.
PhylomeDBi Q64287.
TreeFami TF328512.

Enzyme and pathway databases

Reactomei REACT_198660. Interferon gamma signaling.

Miscellaneous databases

NextBioi 289474.
PROi Q64287.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q64287.
Bgeei Q64287.
CleanExi MM_IRF4.
Genevestigatori Q64287.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProi IPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view ]
PRINTSi PR00267. INTFRNREGFCT.
SMARTi SM00348. IRF. 1 hit.
[Graphical view ]
SUPFAMi SSF49879. SSF49879. 1 hit.
PROSITEi PS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Pip, a novel IRF family member, is a lymphoid-specific, PU.1-dependent transcriptional activator."
    Eisenbeis C.F., Singh H., Storb U.
    Genes Dev. 9:1377-1387(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "Molecular cloning of LSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE)."
    Matsuyama T., Grossman A., Mittruecker H.-W., Siderovski D.P., Kiefer F., Kawakami T., Richardson C.D., Taniguchi T., Yoshinaga S.K., Mak T.W.
    Nucleic Acids Res. 23:2127-2136(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/SvJ and C57BL/6.
    Tissue: Spleen.
  3. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 65-72, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  4. "Phosphorylation of IRF4 by ROCK2 regulates IL-17 and IL-21 production and the development of autoimmunity in mice."
    Biswas P.S., Gupta S., Chang E., Song L., Stirzaker R.A., Liao J.K., Bhagat G., Pernis A.B.
    J. Clin. Invest. 120:3280-3295(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-446 AND SER-447.
  5. Cited for: FUNCTION, INTERACTION WITH BATF.
  6. "BATF-JUN is critical for IRF4-mediated transcription in T cells."
    Li P., Spolski R., Liao W., Wang L., Murphy T.L., Murphy K.M., Leonard W.J.
    Nature 490:543-546(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BATF.
  7. Cited for: FUNCTION, INTERACTION WITH BATF.

Entry informationi

Entry nameiIRF4_MOUSE
AccessioniPrimary (citable) accession number: Q64287
Secondary accession number(s): Q60802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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