Reviewed, UniProtKB/Swiss-Prot Q64285 (CEL_MOUSE)

Names and origin

Protein names

Recommended name:
    Bile salt-activated lipase
      Short name=BAL
    EC=3.1.1.3
    EC=3.1.1.13
Alternative name(s):
    Bile salt-stimulated lipase
      Short name=BSSL
    Carboxyl ester lipase
    Sterol esterase
    Cholesterol esterase
    Pancreatic lysophospholipase

Gene names

Name:	Cel
Synonyms:	Lip1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes fat and vitamin absorption. Acts in concert with pancreatic lipase and colipase for the complete digestion of dietary triglycerides By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate. A steryl ester + H2O = a sterol + a fatty acid.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Repeat Signal
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	ceramide catabolic process Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	sterol esterase activity Inferred from electronic annotation. Source: EC triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Chain	21 – 599	579	Bile salt-activated lipase		PRO_0000008632
Regions
	Repeat	559 – 569	11	1
	Repeat	570 – 580	11	2
	Repeat	581 – 588	8	3
	Region	559 – 588	30	4 X 11 AA tandem repeats, O-glycosylated region
Sites
	Active site	214	1	Acyl-ester intermediate By similarity
	Active site	340	1	Charge relay system By similarity
	Active site	455	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	207	1	N-linked (GlcNAc...) Potential
	Glycosylation	325	1	N-linked (GlcNAc...) Potential
	Disulfide bond	84 ↔ 100		By similarity
	Disulfide bond	266 ↔ 277		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64285-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9E4428FDFCA8602E	FASTA	599	65,813
	10 20 30 40 50 60 MGRLEVLFLG LTCCLAAACA AKLGAVYTEG GFVEGVNKKL SLLGGDSVDI FKGIPFATAK 70 80 90 100 110 120 TLENPQRHPG WQGTLKATNF KKRCLQATIT QDNTYGQEDC LYLNIWVPQG RKQVSHNLPV 130 140 150 160 170 180 MVWIYGGAFL MGSGQGANFL KNYLYDGEEI ATRGNVIVVT FNYRVGPLGF LSTGDANLPG 190 200 210 220 230 240 NFGLRDQHMA IAWVKRNIAA FGGDPDNITI FGESAGAASV SLQTLSPYNK GLIRRAISQS 250 260 270 280 290 300 GMALSPWAIQ KNPLFWAKTI AKKVGCPTED TGKMAACLKI TDPRALTLAY KLPVKKQEYP 310 320 330 340 350 360 VVHYLAFIPV IDGDFIPDDP INLYNNTADI DYIAGINNMD GHLFATIDVP AVDKTKQTVT 370 380 390 400 410 420 EEDFYRLVSG HTVAKGLKGA QATFDIYTES WAQDPSQENM KKTVVAFETD VLFLIPTEIA 430 440 450 460 470 480 LAQHKAHAKS AKTYSYLFSH PSRMPIYPKW MGADHADDLQ YVFGKPFATP LGYRPQDRAV 490 500 510 520 530 540 SKAMIAYWTN FARSGDPNMG NSPVPTHWYP YTLENGNYLD ITKTITSASM KEHLREKFLK 550 560 570 580 590 FWAVTFEVLP TVTGDQDTLT PPEDDSEVAP DPPSDDSQVV PVPPTDDSVE AQMPATIGF

References

[1]	"Characterization of the mouse pancreatic/mammary gland cholesterol esterase-encoding cDNA and gene." Mackay K., Lawn R.M. Gene 165:255-259(1995) [PubMed: 8522186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Mammary gland.
[2]	"Molecular cloning and characterization of the mouse carboxyl ester lipase gene and evidence for expression in the lactating mammary gland." Lidmer A.S., Kannius M., Lundberg L., Bjursell G., Nilsson J. Genomics 29:115-122(1995) [PubMed: 8530060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Lactating mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33169 mRNA. Translation: AAA92088.1. U37386 mRNA. Translation: AAC52279.1.
IPI	IPI00132296.
PIR	A57701.
RefSeq	NP_034015.1.
UniGene	Mm.236017
3D structure databases
SMR	Q64285. Positions 21-553.
ModBase	Search...
Protein-protein interaction databases
STRING	Q64285.
Protein family/group databases
MEROPS	S09.985.
2-D gel databases
SWISS-2DPAGE	Q64285.
Proteomic databases
PRIDE	Q64285.
Genome annotation databases
Ensembl	ENSMUST00000028161; ENSMUSP00000028161; ENSMUSG00000026818; Mus musculus. [Genome view]
GeneID	12613.
KEGG	mmu:12613.
UCSC	uc008iyr.1. mouse.
Organism-specific databases
CTD	12613.
MGI	MGI:88374. Cel.
Phylogenomic databases
HOGENOM	HBG716688.
HOVERGEN	Q64285.
InParanoid	Q64285.
OMA	DIYTESW.
OrthoDB	EOG9PZMSN.
PhylomeDB	Q64285.
Enzyme and pathway databases
BRENDA	3.1.1.13. 244. 3.1.1.3. 244.
Gene expression databases
ArrayExpress	Q64285.
Bgee	Q64285.
CleanEx	MM_CEL.
Genevestigator	Q64285.
GermOnline	ENSMUSG00000026818. Mus musculus.
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. [Graphical view]
PANTHER	PTHR11559. CarbesteraseB. 1 hit.
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	281770.
SOURCE	Search...

Entry information

Entry name

CEL_MOUSE

Accession

Primary (citable) accession number: Q64285

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)