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Protein

Interferon-induced protein with tetratricopeptide repeats 1

Gene

Ifit1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism).2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to exogenous dsRNA Source: InterPro
  • cellular response to interferon-alpha Source: MGI
  • cellular response to interferon-beta Source: MGI
  • cellular response to type I interferon Source: InterPro
  • defense response to virus Source: UniProtKB-KW
  • regulation of defense response to virus Source: InterPro
  • response to virus Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-induced protein with tetratricopeptide repeats 1
Short name:
IFIT-1
Alternative name(s):
Glucocorticoid-attenuated response gene 16 protein
Short name:
GARG-16
Interferon-induced 56 kDa protein
Short name:
IFI-56K
Short name:
P56
Gene namesi
Name:Ifit1
Synonyms:Garg16, Ifi56, Isg56
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:99450. Ifit1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Interferon-induced protein with tetratricopeptide repeats 1PRO_0000106345Add
BLAST

Post-translational modificationi

Phosphorylated.By similarity
ISGylated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ64282.
MaxQBiQ64282.
PaxDbiQ64282.
PRIDEiQ64282.

PTM databases

iPTMnetiQ64282.
PhosphoSiteiQ64282.

Expressioni

Inductioni

By type I interferons, dsRNAs and viruses.1 Publication

Gene expression databases

BgeeiQ64282.
GenevisibleiQ64282. MM.

Interactioni

Subunit structurei

Component of an interferon-dependent multiprotein complex, at least composed of IFIT1, IFIT2 and IFIT3. Interacts with IFIT2 and IFIT3 (By similarity). Interacts with EIF3E (By similarity). Interacts (via TPR repeats 1-4) with RPL15 (By similarity). Interacts with TMEM173/MITA and disrupts its interaction with MAVS or TBK1 (By similarity). Interacts with EIF3C. Interacts (via TPR repeats 4-7) with EEF1A1.By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei34 – 341Interaction with PPP-RNABy similarity
Sitei42 – 421Interaction with PPP-RNABy similarity
Sitei148 – 1481Interaction with PPP-RNABy similarity
Sitei184 – 1841Interaction with PPP-RNABy similarity
Sitei243 – 2431Interaction with PPP-RNABy similarity
Sitei281 – 2811Interaction with the 5'-triphosphate group of PPP-RNABy similarity

Protein-protein interaction databases

IntActiQ64282. 1 interaction.
MINTiMINT-4124109.
STRINGi10090.ENSMUSP00000099888.

Structurei

3D structure databases

ProteinModelPortaliQ64282.
SMRiQ64282. Positions 8-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati52 – 8534TPR 1Add
BLAST
Repeati92 – 12635TPR 2Add
BLAST
Repeati136 – 17136TPR 3Add
BLAST
Repeati174 – 20734TPR 4Add
BLAST
Repeati209 – 24133TPR 5Add
BLAST
Repeati242 – 27534TPR 6Add
BLAST
Repeati294 – 32835TPR 7Add
BLAST
Repeati329 – 36234TPR 8Add
BLAST
Repeati367 – 40135TPR 9Add
BLAST
Repeati426 – 45934TPR 10Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni247 – 2537Interaction with the 5'-triphosphate group of PPP-RNABy similarity

Domaini

RNA recognition is mediated by a convoluted intramolecular fold of the TPR repeats (TPR eddy), which scaffolds unique additional helices that form an RNA binding cleft.By similarity

Sequence similaritiesi

Belongs to the IFIT family.Curated
Contains 10 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00390000013876.
HOGENOMiHOG000001558.
HOVERGENiHBG066330.
InParanoidiQ64282.
KOiK14217.
OMAiFEMAYVC.
OrthoDBiEOG71K62V.
TreeFamiTF342671.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR024121. Interferon-induced_IFIT1.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10271:SF16. PTHR10271:SF16. 1 hit.
PfamiPF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 6 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 4 hits.
PROSITEiPS50005. TPR. 4 hits.
PS50293. TPR_REGION. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64282-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGENADGDQV MENLLQLRCH FTWKLLFENN DIPDLEVRIS EQVQFLDIKN
60 70 80 90 100
PLGMHNLLAY VRHLKGQQDE ALQSLKEAEA LIQSEQLSKR SLATWGNCAW
110 120 130 140 150
LHYHRGSLAE AQIYLDKVEK VCKEFSSPFR YRLECAEMDC EEGWALLKCG
160 170 180 190 200
GGNYKQAMAC FAKALKVEPE NPEYNTGYAV VAYRQDLDDN FISLEPLRKA
210 220 230 240 250
VRLNPEDPYL KVLLALKLQD LGEHVEAEAH IEEALSSTSC QSYVIRYAAK
260 270 280 290 300
YFRRKHRVDK ALHLLNRALQ ASPSSGYLHY QKGLCYKQQI SQLRTSRNRQ
310 320 330 340 350
PRRQDNVQEL AQQAIHEFQE TLKLRPTFEM AYVCMAEVQA EIHQYEEAER
360 370 380 390 400
NFQKALNNKT LVAHIEQDIH LRYGRFLQFH KQSEDKAITL YLKGLKVEEK
410 420 430 440 450
SFAWRKLLTA LEKVAERRVC QNVHLVESTS LLGLVYKLKG QEKNALFYYE
460
KALRLTGEMN PAF
Length:463
Mass (Da):53,737
Last modified:July 27, 2011 - v2
Checksum:i166FAB371F7B6D2A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131I → V in AAB33831 (PubMed:7896268).Curated
Sequence conflicti113 – 1131I → V in AAC52625 (PubMed:8660659).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S77715, S77714 Genomic DNA. Translation: AAB33831.2.
U43084 mRNA. Translation: AAC52625.1.
AK150352 mRNA. Translation: BAE29489.1.
AK150424 mRNA. Translation: BAE29547.1.
AK150609 mRNA. Translation: BAE29700.1.
AK151125 mRNA. Translation: BAE30133.1.
AK151158 mRNA. Translation: BAE30163.1.
AK151278 mRNA. Translation: BAE30265.1.
AK151298 mRNA. Translation: BAE30281.1.
AK153184 mRNA. Translation: BAE31785.1.
AK157848 mRNA. Translation: BAE34228.1.
CT010226 mRNA. Translation: CAJ18434.1.
CH466534 Genomic DNA. Translation: EDL41756.1.
BC003768 mRNA. Translation: AAH03768.1.
CCDSiCCDS29766.1.
PIRiB55508.
RefSeqiNP_032357.2. NM_008331.3.
UniGeneiMm.439751.

Genome annotation databases

EnsembliENSMUST00000102824; ENSMUSP00000099888; ENSMUSG00000034459.
GeneIDi15957.
KEGGimmu:15957.
UCSCiuc008hgt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S77715, S77714 Genomic DNA. Translation: AAB33831.2.
U43084 mRNA. Translation: AAC52625.1.
AK150352 mRNA. Translation: BAE29489.1.
AK150424 mRNA. Translation: BAE29547.1.
AK150609 mRNA. Translation: BAE29700.1.
AK151125 mRNA. Translation: BAE30133.1.
AK151158 mRNA. Translation: BAE30163.1.
AK151278 mRNA. Translation: BAE30265.1.
AK151298 mRNA. Translation: BAE30281.1.
AK153184 mRNA. Translation: BAE31785.1.
AK157848 mRNA. Translation: BAE34228.1.
CT010226 mRNA. Translation: CAJ18434.1.
CH466534 Genomic DNA. Translation: EDL41756.1.
BC003768 mRNA. Translation: AAH03768.1.
CCDSiCCDS29766.1.
PIRiB55508.
RefSeqiNP_032357.2. NM_008331.3.
UniGeneiMm.439751.

3D structure databases

ProteinModelPortaliQ64282.
SMRiQ64282. Positions 8-458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ64282. 1 interaction.
MINTiMINT-4124109.
STRINGi10090.ENSMUSP00000099888.

PTM databases

iPTMnetiQ64282.
PhosphoSiteiQ64282.

Proteomic databases

EPDiQ64282.
MaxQBiQ64282.
PaxDbiQ64282.
PRIDEiQ64282.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102824; ENSMUSP00000099888; ENSMUSG00000034459.
GeneIDi15957.
KEGGimmu:15957.
UCSCiuc008hgt.1. mouse.

Organism-specific databases

CTDi3434.
MGIiMGI:99450. Ifit1.

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00390000013876.
HOGENOMiHOG000001558.
HOVERGENiHBG066330.
InParanoidiQ64282.
KOiK14217.
OMAiFEMAYVC.
OrthoDBiEOG71K62V.
TreeFamiTF342671.

Miscellaneous databases

PROiQ64282.
SOURCEiSearch...

Gene expression databases

BgeeiQ64282.
GenevisibleiQ64282. MM.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR024121. Interferon-induced_IFIT1.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10271:SF16. PTHR10271:SF16. 1 hit.
PfamiPF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 6 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 4 hits.
PROSITEiPS50005. TPR. 4 hits.
PS50293. TPR_REGION. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure, chromosome localization, and regulation of expression of the interferon-regulated mouse Ifi54/Ifi56 gene family."
    Bluyssen H.A., Vlietstra R.J., Faber P.W., Smit E.M., Hagemeijer A., Trapman J.
    Genomics 24:137-148(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The glucocorticoid attenuated response genes GARG-16, GARG-39, and GARG-49/IRG2 encode inducible proteins containing multiple tetratricopeptide repeat domains."
    Smith J.B., Herschman H.R.
    Arch. Biochem. Biophys. 330:290-300(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow.
  4. "Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)."
    Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  7. "Induction and mode of action of the viral stress-inducible murine proteins, P56 and P54."
    Terenzi F., Pal S., Sen G.C.
    Virology 340:116-124(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EIF3C.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Lung and Spleen.
  9. "The interaction between interferon-induced protein with tetratricopeptide repeats-1 and eukaryotic elongation factor-1A."
    Li H.T., Su Y.P., Cheng T.M., Xu J.M., Liao J., Chen J.C., Ji C.Y., Ai G.P., Wang J.P.
    Mol. Cell. Biochem. 337:101-110(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EEF1A1, SUBCELLULAR LOCATION.
  10. Cited for: FUNCTION.
  11. Cited for: REVIEW.
  12. "2'-o methylation of the viral mRNA cap by west nile virus evades ifit1-dependent and -independent mechanisms of host restriction in vivo."
    Szretter K.J., Daniels B.P., Cho H., Gainey M.D., Yokoyama W.M., Gale M. Jr., Virgin H.W., Klein R.S., Sen G.C., Diamond M.S.
    PLoS Pathog. 8:E1002698-E1002698(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiIFIT1_MOUSE
AccessioniPrimary (citable) accession number: Q64282
Secondary accession number(s): Q99L77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.