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Protein

Leukocyte immunoglobulin-like receptor subfamily B member 4

Gene

Lilrb4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for class I MHC antigens. Involved in the down-regulation of the immune response and the development of tolerance. Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Adaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Leukocyte immunoglobulin-like receptor subfamily B member 4
Alternative name(s):
Mast cell surface glycoprotein Gp49B
CD_antigen: CD85k
Gene namesi
Name:Lilrb4
Synonyms:Gp49b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:102701. Lilrb4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 238215ExtracellularSequence analysisAdd
BLAST
Transmembranei239 – 26022HelicalSequence analysisAdd
BLAST
Topological domaini261 – 33575CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323By similarityAdd
BLAST
Chaini24 – 335312Leukocyte immunoglobulin-like receptor subfamily B member 4PRO_0000014824Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 98PROSITE-ProRule annotation
Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence analysis
Disulfide bondi144 ↔ 196PROSITE-ProRule annotation
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ64281.
MaxQBiQ64281.
PaxDbiQ64281.
PRIDEiQ64281.

PTM databases

PhosphoSiteiQ64281.

Expressioni

Gene expression databases

BgeeiQ64281.
GenevisibleiQ64281. MM.

Interactioni

Subunit structurei

Binds PTPN6 when phosphorylated.By similarity

Protein-protein interaction databases

BioGridi200008. 2 interactions.
DIPiDIP-46122N.
STRINGi10090.ENSMUSP00000077833.

Structurei

3D structure databases

ProteinModelPortaliQ64281.
SMRiQ64281. Positions 26-219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 12584Ig-like C2-type 1Add
BLAST
Domaini124 – 21289Ig-like C2-type 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi298 – 3036ITIM motif 1
Motifi320 – 3256ITIM motif 2

Domaini

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IKJD. Eukaryota.
ENOG41116BR. LUCA.
GeneTreeiENSGT00760000119033.
HOGENOMiHOG000234395.
HOVERGENiHBG074353.
InParanoidiQ64281.
OMAiCYYESAA.
OrthoDBiEOG7R56S3.
PhylomeDBiQ64281.
TreeFamiTF336644.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF13895. Ig_2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q64281-1) [UniParc]FASTAAdd to basket

Also known as: GP49B1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIAMLTVLLY LGLILEPRTA VQAGHLPKPI IWAEPGSVIA AYTSVITWCQ
60 70 80 90 100
GSWEAQYYHL YKEKSVNPWD TQVPLETRNK AKFNIPSMTT SYAGIYKCYY
110 120 130 140 150
ESAAGFSEHS DAMELVMTGA YENPSLSVYP SSNVTSGVSI SFSCSSSIVF
160 170 180 190 200
GRFILIQEGK HGLSWTLDSQ HQANQPSYAT FVLDAVTPNH NGTFRCYGYF
210 220 230 240 250
RNEPQVWSKP SNSLDLMISE TKDQSSTPTE DGLETYQKIL IGVLVSFLLL
260 270 280 290 300
FFLLLFLILI GYQYGHKKKA NASVKNTQSE NNAELNSWNP QNEDPQGIVY
310 320 330
AQVKPSRLQK DTACKETQDV TYAQLCIRTQ EQNNS
Length:335
Mass (Da):37,544
Last modified:November 1, 1996 - v1
Checksum:i6005186D524E7876
GO
Isoform 2 (identifier: Q64281-2) [UniParc]FASTAAdd to basket

Also known as: GP49B2

The sequence of this isoform differs from the canonical sequence as follows:
     232-232: G → D
     233-271: Missing.

Show »
Length:296
Mass (Da):33,049
Checksum:i6CEB69F4A75EA1EC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei232 – 2321G → D in isoform 2. 1 PublicationVSP_002510
Alternative sequencei233 – 27139Missing in isoform 2. 1 PublicationVSP_002511Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05266 mRNA. Translation: AAA17799.1.
U05265 Unassigned DNA. Translation: AAA17797.1.
U05265 Unassigned DNA. Translation: AAA17798.1.
U05264 mRNA. Translation: AAA17796.1.
BC006591 mRNA. Translation: AAH06591.1.
CCDSiCCDS23832.1. [Q64281-1]
PIRiA53434.
B53434.
RefSeqiNP_038560.1. NM_013532.3. [Q64281-1]
UniGeneiMm.34408.

Genome annotation databases

EnsembliENSMUST00000078778; ENSMUSP00000077833; ENSMUSG00000062593. [Q64281-1]
ENSMUST00000105481; ENSMUSP00000101121; ENSMUSG00000062593. [Q64281-1]
ENSMUST00000105482; ENSMUSP00000101122; ENSMUSG00000062593. [Q64281-2]
GeneIDi14728.
KEGGimmu:14728.
UCSCiuc007far.2. mouse. [Q64281-1]
uc011xdq.2. mouse. [Q64281-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05266 mRNA. Translation: AAA17799.1.
U05265 Unassigned DNA. Translation: AAA17797.1.
U05265 Unassigned DNA. Translation: AAA17798.1.
U05264 mRNA. Translation: AAA17796.1.
BC006591 mRNA. Translation: AAH06591.1.
CCDSiCCDS23832.1. [Q64281-1]
PIRiA53434.
B53434.
RefSeqiNP_038560.1. NM_013532.3. [Q64281-1]
UniGeneiMm.34408.

3D structure databases

ProteinModelPortaliQ64281.
SMRiQ64281. Positions 26-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200008. 2 interactions.
DIPiDIP-46122N.
STRINGi10090.ENSMUSP00000077833.

PTM databases

PhosphoSiteiQ64281.

Proteomic databases

EPDiQ64281.
MaxQBiQ64281.
PaxDbiQ64281.
PRIDEiQ64281.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078778; ENSMUSP00000077833; ENSMUSG00000062593. [Q64281-1]
ENSMUST00000105481; ENSMUSP00000101121; ENSMUSG00000062593. [Q64281-1]
ENSMUST00000105482; ENSMUSP00000101122; ENSMUSG00000062593. [Q64281-2]
GeneIDi14728.
KEGGimmu:14728.
UCSCiuc007far.2. mouse. [Q64281-1]
uc011xdq.2. mouse. [Q64281-2]

Organism-specific databases

CTDi14728.
MGIiMGI:102701. Lilrb4.

Phylogenomic databases

eggNOGiENOG410IKJD. Eukaryota.
ENOG41116BR. LUCA.
GeneTreeiENSGT00760000119033.
HOGENOMiHOG000234395.
HOVERGENiHBG074353.
InParanoidiQ64281.
OMAiCYYESAA.
OrthoDBiEOG7R56S3.
PhylomeDBiQ64281.
TreeFamiTF336644.

Miscellaneous databases

NextBioi286761.
PROiQ64281.
SOURCEiSearch...

Gene expression databases

BgeeiQ64281.
GenevisibleiQ64281. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF13895. Ig_2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the gp49B gene of the immunoglobulin superfamily and demonstration that one of its two products is an early-expressed mast cell surface protein originally described as gp49."
    Castells M.C., Wu X., Arm J.P., Austen K.F., Katz H.R.
    J. Biol. Chem. 269:8393-8401(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: BALB/cJ and C3H.
    Tissue: Bone marrow.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLIRB4_MOUSE
AccessioniPrimary (citable) accession number: Q64281
Secondary accession number(s): Q64312
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.