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Protein

Left-right determination factor 1

Gene

Lefty1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for left-right axis determination as a regulator of LEFTY2 and NODAL.1 Publication

GO - Molecular functioni

  • cytokine activity Source: GO_Central
  • nodal binding Source: UniProtKB
  • transforming growth factor beta receptor binding Source: GO_Central

GO - Biological processi

  • anterior/posterior axis specification Source: MGI
  • cell development Source: GO_Central
  • cell growth Source: InterPro
  • cell migration involved in gastrulation Source: MGI
  • determination of left/right symmetry Source: BHF-UCL
  • heart morphogenesis Source: BHF-UCL
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of nodal receptor complex assembly Source: UniProtKB
  • negative regulation of nodal signaling pathway Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of pathway-restricted SMAD protein phosphorylation Source: GO_Central
  • regulation of apoptotic process Source: GO_Central
  • regulation of MAPK cascade Source: GO_Central
  • response to retinoic acid Source: MGI
  • SMAD protein signal transduction Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Developmental protein, Growth factor

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-1433617. Regulation of signaling by NODAL.

Names & Taxonomyi

Protein namesi
Recommended name:
Left-right determination factor 1
Alternative name(s):
Protein lefty-1
Short name:
Lefty protein
Stimulated by retinoic acid gene 3 protein
Transforming growth factor beta-4
Short name:
TGF-beta-4
Gene namesi
Name:Lefty1
Synonyms:Ebaf, Lefty, Stra3, Tgfb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:107405. Lefty1.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • extracellular space Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Propeptidei22 – 7655Or 135Sequence analysisPRO_0000033808Add
BLAST
Chaini77 – 368292Left-right determination factor 1PRO_0000033809Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence analysis
Disulfide bondi253 ↔ 266By similarity
Disulfide bondi265 ↔ 318By similarity
Disulfide bondi295 ↔ 353By similarity
Disulfide bondi299 ↔ 355By similarity

Post-translational modificationi

The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ64280.
PeptideAtlasiQ64280.
PRIDEiQ64280.

PTM databases

PhosphoSiteiQ64280.

Expressioni

Developmental stagei

By E8.0, expressed exclusively on the left side of developing embryos with expression predominantly in the prospective floor plate (PFP). Weak expression in the lateral-plate mesoderm (LPM).

Gene expression databases

BgeeiENSMUSG00000038793.
CleanExiMM_LEFTY1.
GenevisibleiQ64280. MM.

Interactioni

GO - Molecular functioni

  • cytokine activity Source: GO_Central
  • nodal binding Source: UniProtKB
  • transforming growth factor beta receptor binding Source: GO_Central

Protein-protein interaction databases

DIPiDIP-46067N.
STRINGi10090.ENSMUSP00000041427.

Structurei

3D structure databases

ProteinModelPortaliQ64280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00390000010056.
HOGENOMiHOG000113317.
HOVERGENiHBG074429.
InParanoidiQ64280.
KOiK04668.
OMAiHYINFRE.
OrthoDBiEOG091G09EN.
PhylomeDBiQ64280.
TreeFamiTF106462.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR003942. LRDF.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
PIRSFiPIRSF037402. TGFb4. 1 hit.
PRINTSiPR01427. TGFBETA4.
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFLWLCWAL WALSLVSLRE ALTGEQILGS LLQQLQLDQP PVLDKADVEG
60 70 80 90 100
MVIPSHVRTQ YVALLQHSHA SRSRGKRFSQ NLREVAGRFL VSETSTHLLV
110 120 130 140 150
FGMEQRLPPN SELVQAVLRL FQEPVPRTAL RRQKRLSPHS ARARVTIEWL
160 170 180 190 200
RFRDDGSNRT ALIDSRLVSI HESGWKAFDV TEAVNFWQQL SRPRQPLLLQ
210 220 230 240 250
VSVQREHLGP GTWSSHKLVR FAAQGTPDGK GQGEPQLELH TLDLKDYGAQ
260 270 280 290 300
GNCDPEAPVT EGTRCCRQEM YLDLQGMKWA ENWILEPPGF LTYECVGSCL
310 320 330 340 350
QLPESLTSRW PFLGPRQCVA SEMTSLPMIV SVKEGGRTRP QVVSLPNMRV
360
QTCSCASDGA LIPRRLQP
Length:368
Mass (Da):41,498
Last modified:November 1, 1996 - v1
Checksum:i821DAE663C546B5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83921 mRNA. Translation: BAA12121.1.
Z73151 mRNA. Translation: CAA97497.1.
AJ000082 mRNA. Translation: CAA03909.1.
AJ000083 Genomic DNA. Translation: CAA03910.1.
AK131943 mRNA. Translation: BAE20889.1.
BC050221 mRNA. Translation: AAH50221.1.
CCDSiCCDS15577.1.
PIRiS67507.
RefSeqiNP_034224.1. NM_010094.3.
UniGeneiMm.378911.

Genome annotation databases

EnsembliENSMUST00000037361; ENSMUSP00000041427; ENSMUSG00000038793.
GeneIDi13590.
KEGGimmu:13590.
UCSCiuc007dww.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83921 mRNA. Translation: BAA12121.1.
Z73151 mRNA. Translation: CAA97497.1.
AJ000082 mRNA. Translation: CAA03909.1.
AJ000083 Genomic DNA. Translation: CAA03910.1.
AK131943 mRNA. Translation: BAE20889.1.
BC050221 mRNA. Translation: AAH50221.1.
CCDSiCCDS15577.1.
PIRiS67507.
RefSeqiNP_034224.1. NM_010094.3.
UniGeneiMm.378911.

3D structure databases

ProteinModelPortaliQ64280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46067N.
STRINGi10090.ENSMUSP00000041427.

PTM databases

PhosphoSiteiQ64280.

Proteomic databases

PaxDbiQ64280.
PeptideAtlasiQ64280.
PRIDEiQ64280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037361; ENSMUSP00000041427; ENSMUSG00000038793.
GeneIDi13590.
KEGGimmu:13590.
UCSCiuc007dww.1. mouse.

Organism-specific databases

CTDi10637.
MGIiMGI:107405. Lefty1.

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00390000010056.
HOGENOMiHOG000113317.
HOVERGENiHBG074429.
InParanoidiQ64280.
KOiK04668.
OMAiHYINFRE.
OrthoDBiEOG091G09EN.
PhylomeDBiQ64280.
TreeFamiTF106462.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-1433617. Regulation of signaling by NODAL.

Miscellaneous databases

PROiQ64280.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038793.
CleanExiMM_LEFTY1.
GenevisibleiQ64280. MM.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR003942. LRDF.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
PIRSFiPIRSF037402. TGFb4. 1 hit.
PRINTSiPR01427. TGFBETA4.
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLFTY1_MOUSE
AccessioniPrimary (citable) accession number: Q64280
Secondary accession number(s): Q3V2A9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.