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Protein

Left-right determination factor 1

Gene

Lefty1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required for left-right axis determination as a regulator of LEFTY2 and NODAL.1 Publication

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior axis specification Source: MGI
  • BMP signaling pathway Source: GO_Central
  • cell development Source: GO_Central
  • cell migration involved in gastrulation Source: MGI
  • determination of left/right symmetry Source: BHF-UCL
  • heart morphogenesis Source: BHF-UCL
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of nodal receptor complex assembly Source: UniProtKB
  • negative regulation of nodal signaling pathway Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: BHF-UCL
  • positive regulation of pathway-restricted SMAD protein phosphorylation Source: GO_Central
  • regulation of apoptotic process Source: GO_Central
  • regulation of MAPK cascade Source: GO_Central
  • response to retinoic acid Source: MGI
  • SMAD protein signal transduction Source: GO_Central

Keywordsi

Molecular functionCytokine, Developmental protein, Growth factor

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Left-right determination factor 1
Alternative name(s):
Protein lefty-1
Short name:
Lefty protein
Stimulated by retinoic acid gene 3 protein
Transforming growth factor beta-4
Short name:
TGF-beta-4
Gene namesi
Name:Lefty1
Synonyms:Ebaf, Lefty, Stra3, Tgfb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:107405 Lefty1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000003380822 – 76Or 135Sequence analysisAdd BLAST55
ChainiPRO_000003380977 – 368Left-right determination factor 1Add BLAST292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi158N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi253 ↔ 266By similarity
Disulfide bondi265 ↔ 318By similarity
Disulfide bondi295 ↔ 353By similarity
Disulfide bondi299 ↔ 355By similarity

Post-translational modificationi

The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ64280
PeptideAtlasiQ64280
PRIDEiQ64280

PTM databases

PhosphoSitePlusiQ64280

Expressioni

Developmental stagei

By E8.0, expressed exclusively on the left side of developing embryos with expression predominantly in the prospective floor plate (PFP). Weak expression in the lateral-plate mesoderm (LPM).

Gene expression databases

BgeeiENSMUSG00000038793
CleanExiMM_LEFTY1
GenevisibleiQ64280 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-46067N
IntActiQ64280, 1 interactor
STRINGi10090.ENSMUSP00000041427

Structurei

3D structure databases

ProteinModelPortaliQ64280
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3900 Eukaryota
ENOG410XT8Z LUCA
GeneTreeiENSGT00390000010056
HOGENOMiHOG000113317
HOVERGENiHBG074429
InParanoidiQ64280
KOiK04668
OMAiETGWKSF
OrthoDBiEOG091G09EN
PhylomeDBiQ64280
TreeFamiTF106462

Family and domain databases

Gene3Di2.10.90.10, 1 hit
InterProiView protein in InterPro
IPR029034 Cystine-knot_cytokine
IPR003942 LRDF
IPR001839 TGF-b_C
IPR001111 TGF-b_propeptide
IPR015615 TGF-beta-rel
IPR017948 TGFb_CS
PANTHERiPTHR11848 PTHR11848, 1 hit
PfamiView protein in Pfam
PF00019 TGF_beta, 1 hit
PF00688 TGFb_propeptide, 1 hit
PIRSFiPIRSF037402 TGFb4, 1 hit
PRINTSiPR01427 TGFBETA4
SMARTiView protein in SMART
SM00204 TGFB, 1 hit
SUPFAMiSSF57501 SSF57501, 1 hit
PROSITEiView protein in PROSITE
PS00250 TGF_BETA_1, 1 hit
PS51362 TGF_BETA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFLWLCWAL WALSLVSLRE ALTGEQILGS LLQQLQLDQP PVLDKADVEG
60 70 80 90 100
MVIPSHVRTQ YVALLQHSHA SRSRGKRFSQ NLREVAGRFL VSETSTHLLV
110 120 130 140 150
FGMEQRLPPN SELVQAVLRL FQEPVPRTAL RRQKRLSPHS ARARVTIEWL
160 170 180 190 200
RFRDDGSNRT ALIDSRLVSI HESGWKAFDV TEAVNFWQQL SRPRQPLLLQ
210 220 230 240 250
VSVQREHLGP GTWSSHKLVR FAAQGTPDGK GQGEPQLELH TLDLKDYGAQ
260 270 280 290 300
GNCDPEAPVT EGTRCCRQEM YLDLQGMKWA ENWILEPPGF LTYECVGSCL
310 320 330 340 350
QLPESLTSRW PFLGPRQCVA SEMTSLPMIV SVKEGGRTRP QVVSLPNMRV
360
QTCSCASDGA LIPRRLQP
Length:368
Mass (Da):41,498
Last modified:November 1, 1996 - v1
Checksum:i821DAE663C546B5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83921 mRNA Translation: BAA12121.1
Z73151 mRNA Translation: CAA97497.1
AJ000082 mRNA Translation: CAA03909.1
AJ000083 Genomic DNA Translation: CAA03910.1
AK131943 mRNA Translation: BAE20889.1
BC050221 mRNA Translation: AAH50221.1
CCDSiCCDS15577.1
PIRiS67507
RefSeqiNP_034224.1, NM_010094.4
UniGeneiMm.378911

Genome annotation databases

EnsembliENSMUST00000037361; ENSMUSP00000041427; ENSMUSG00000038793
GeneIDi13590
KEGGimmu:13590
UCSCiuc007dww.1 mouse

Similar proteinsi

Entry informationi

Entry nameiLFTY1_MOUSE
AccessioniPrimary (citable) accession number: Q64280
Secondary accession number(s): Q3V2A9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health