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Q64273

- KCNJ2_RAT

UniProt

Q64273 - KCNJ2_RAT

Protein

Inward rectifier potassium channel 2

Gene

Kcnj2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei172 – 1721Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    GO - Molecular functioni

    1. inward rectifier potassium channel activity Source: UniProtKB
    2. phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
    3. voltage-gated potassium channel activity involved in cardiac muscle cell action potential repolarization Source: Ensembl

    GO - Biological processi

    1. cardiac muscle cell action potential involved in contraction Source: Ensembl
    2. cellular response to mechanical stimulus Source: RGD
    3. magnesium ion transport Source: Ensembl
    4. positive regulation of potassium ion transmembrane transport Source: RGD
    5. potassium ion import Source: Ensembl
    6. potassium ion transport Source: UniProtKB
    7. protein homotetramerization Source: UniProtKB
    8. regulation of cardiac muscle cell contraction Source: RGD
    9. regulation of heart rate by cardiac conduction Source: Ensembl
    10. regulation of membrane repolarization Source: Ensembl
    11. regulation of skeletal muscle contraction via regulation of action potential Source: Ensembl
    12. relaxation of cardiac muscle Source: Ensembl
    13. relaxation of skeletal muscle Source: Ensembl

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199207. Classical Kir channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inward rectifier potassium channel 2
    Alternative name(s):
    Inward rectifier K(+) channel Kir2.1
    Short name:
    IRK-1
    Short name:
    RBL-IRK1
    Potassium channel, inwardly rectifying subfamily J member 2
    Gene namesi
    Name:Kcnj2
    Synonyms:Irk1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi61968. Kcnj2.

    Subcellular locationi

    GO - Cellular componenti

    1. dendrite Source: RGD
    2. dendritic spine Source: RGD
    3. Golgi apparatus Source: RGD
    4. intercalated disc Source: RGD
    5. intrinsic component of membrane Source: UniProtKB
    6. neuronal cell body Source: RGD
    7. plasma membrane Source: RGD
    8. rough endoplasmic reticulum Source: RGD
    9. smooth endoplasmic reticulum Source: RGD
    10. T-tubule Source: RGD
    11. voltage-gated potassium channel complex Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427Inward rectifier potassium channel 2PRO_0000154928Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei76 – 761S-nitrosocysteineBy similarity

    Post-translational modificationi

    S-nitrosylation increases the open probabilty and inward rectifying currents.By similarity

    Keywords - PTMi

    S-nitrosylation

    Proteomic databases

    PaxDbiQ64273.

    Expressioni

    Tissue specificityi

    Prominently expressed in the central nervous system. Also found in other excitable tissues such as heart and skeletal muscle.

    Gene expression databases

    GenevestigatoriQ64273.

    Interactioni

    Subunit structurei

    Homomultimeric and heteromultimeric association with KCNJ4/Kir2.3. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking.

    Protein-protein interaction databases

    DIPiDIP-33299N.
    IntActiQ64273. 1 interaction.
    STRINGi10116.ENSRNOP00000006254.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64273.
    SMRiQ64273. Positions 187-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8181CytoplasmicBy similarityAdd
    BLAST
    Topological domaini107 – 12822ExtracellularBy similarityAdd
    BLAST
    Topological domaini148 – 1569ExtracellularBy similarity
    Topological domaini179 – 427249CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei129 – 14012Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei141 – 1477Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei82 – 10625Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei157 – 17822Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi142 – 1476Selectivity filterBy similarity
    Motifi425 – 4273PDZ-bindingSequence Analysis

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG72812.
    GeneTreeiENSGT00650000093228.
    HOGENOMiHOG000237325.
    HOVERGENiHBG006178.
    InParanoidiQ64273.
    KOiK04996.
    OMAiHNATVAM.
    OrthoDBiEOG7XPZ5K.
    PhylomeDBiQ64273.
    TreeFamiTF313676.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003271. K_chnl_inward-rec_Kir2.1.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    IPR013673. K_chnl_inward-rec_Kir_N.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    PF08466. IRK_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01324. KIR21CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q64273-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSVRTNRYS IVSSEEDGMK LATMAVANGF GNGKSKVHTR QQCRSRFVKK    50
    DGHCNVQFIN VGEKGQRYLA DIFTTCVDIR WRWMLVIFCL AFVLSWLFFG 100
    CVFWLIALLH GDLDASKESK ACVSEVNSFT AAFLFSIETQ TTIGYGFRCV 150
    TDECPIAVFM VVFQSIVGCI IDAFIIGAVM AKMAKPKKRN ETLVFSHNAV 200
    IAMRDGKLCL MWRVGNLRKS HLVEAHVRAQ LLKSRITSEG EYIPLDQIDI 250
    NVGFDSGIDR IFLVSPITIV HEIDEDSPLY DLSKQDIDNA DFEIVVILEG 300
    MVEATAMTTQ CRSSYLANEI LWGHRYEPVL FEEKHCYKVD YSRFHKTYEV 350
    PNTPLCSARD LAEKKYILSN ANSFCYENEV ALTSKEEEDS ENGVPESTST 400
    DSPPGIDLHN QASVPLEPRP LRRESEI 427
    Length:427
    Mass (Da):48,200
    Last modified:November 1, 1996 - v1
    Checksum:i85538FB1CCEBF8ED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L48490 mRNA. Translation: AAB03890.1.
    AF021137 mRNA. Translation: AAB88795.1.
    RefSeqiNP_058992.1. NM_017296.1.
    XP_006247629.1. XM_006247567.1.
    UniGeneiRn.44415.

    Genome annotation databases

    EnsembliENSRNOT00000006254; ENSRNOP00000006254; ENSRNOG00000004720.
    GeneIDi29712.
    KEGGirno:29712.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L48490 mRNA. Translation: AAB03890.1 .
    AF021137 mRNA. Translation: AAB88795.1 .
    RefSeqi NP_058992.1. NM_017296.1.
    XP_006247629.1. XM_006247567.1.
    UniGenei Rn.44415.

    3D structure databases

    ProteinModelPortali Q64273.
    SMRi Q64273. Positions 187-370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-33299N.
    IntActi Q64273. 1 interaction.
    STRINGi 10116.ENSRNOP00000006254.

    Proteomic databases

    PaxDbi Q64273.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000006254 ; ENSRNOP00000006254 ; ENSRNOG00000004720 .
    GeneIDi 29712.
    KEGGi rno:29712.

    Organism-specific databases

    CTDi 3759.
    RGDi 61968. Kcnj2.

    Phylogenomic databases

    eggNOGi NOG72812.
    GeneTreei ENSGT00650000093228.
    HOGENOMi HOG000237325.
    HOVERGENi HBG006178.
    InParanoidi Q64273.
    KOi K04996.
    OMAi HNATVAM.
    OrthoDBi EOG7XPZ5K.
    PhylomeDBi Q64273.
    TreeFami TF313676.

    Enzyme and pathway databases

    Reactomei REACT_199207. Classical Kir channels.

    Miscellaneous databases

    NextBioi 610149.
    PROi Q64273.

    Gene expression databases

    Genevestigatori Q64273.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003271. K_chnl_inward-rec_Kir2.1.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    IPR013673. K_chnl_inward-rec_Kir_N.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    PF08466. IRK_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01324. KIR21CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Physiological and molecular characterization of an IRK-type inward rectifier K+ channel in a tumour mast cell line."
      Wischmeyer E., Lentes K.U., Karschin A.
      Pflugers Arch. 429:809-819(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Inwardly rectifying potassium channels in lens epithelium are from the IRK1 (Kir 2.1) family."
      Rae J.L., Shepard A.R.
      Exp. Eye Res. 66:347-359(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Lens epithelium.
    3. "A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels."
      Leonoudakis D., Conti L.R., Radeke C.M., McGuire L.M., Vandenberg C.A.
      J. Biol. Chem. 279:19051-19063(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CASK; LIN7A; LIN7B; LIN7C; APBA1 AND DLG1, FUNCTION.

    Entry informationi

    Entry nameiKCNJ2_RAT
    AccessioniPrimary (citable) accession number: Q64273
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3