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Q64268 (HEP2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heparin cofactor 2
Alternative name(s):
Heparin cofactor II
Short name=HC-II
Protease inhibitor leuserpin-2
Serpin D1
Gene names
Name:Serpind1
Synonyms:Hcf2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT) By similarity.

Domain

The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition By similarity.

Post-translational modification

Different composition of the N-linked oligosaccharides appears to yield a 68-kDa and a 72-kDa form By similarity.

Sequence similarities

Belongs to the serpin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 479456Heparin cofactor 2
PRO_0000032497

Regions

Repeat55 – 65111
Repeat69 – 79112
Region55 – 79252 X 11 AA approximate repeats, Asp/Glu-rich (acidic) (hirudin-like)
Region172 – 19221Glycosaminoglycan-binding site By similarity

Sites

Site443 – 4442Reactive bond By similarity

Amino acid modifications

Modified residue611Sulfotyrosine By similarity
Modified residue741Sulfotyrosine By similarity
Glycosylation311N-linked (GlcNAc...) Potential
Glycosylation1681N-linked (GlcNAc...) Potential
Glycosylation3671N-linked (GlcNAc...) Potential
Glycosylation4031N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q64268 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 4F736C2F8459E514

FASTA47954,552
        10         20         30         40         50         60 
MKHPAYTLLL SLIMSMCAGS KGLAEQLTKE NLTVSLLPPN FHKENTVTND WIPEGEEDDD 

        70         80         90        100        110        120 
YLDLEKLLSE DDDYIYVVDA VSPTDSESSA GNILQLFQGK SRIQRLNILN AKFAFNLYRV 

       130        140        150        160        170        180 
LKDQATSSDN IFIAPVGIST AMGMISLGLR GETHEEVHSV LHFKDFVNAS SKYEVTTIHN 

       190        200        210        220        230        240 
LFRKLTHRLF RRNFGYTLQS VNDLYIQKQF PIREDFKAAM REFYFAEAQE ADFSDPAFIS 

       250        260        270        280        290        300 
KANSHILKLT KGLIKEALEN TDSATQMMIL NCIYFKGAWM NKFPVEMTHN HNFRLNEREV 

       310        320        330        340        350        360 
VKVSMMQTKG NFLAANDQEL DCDILQLEYV GGISMLIVIP RKLSGMKTLE AQLTPQVVER 

       370        380        390        400        410        420 
WQKSMTNRTR EVLLPKFKLE KNYNLVEVLK SMGITKLFNK NGNMSGISDQ RIIIDLFKHQ 

       430        440        450        460        470 
STITVNEEGT QAAAVTTVGF MPLSTQVRFT VDRPFLFLVY EHRTSCLLFM GRVANPAKS 

« Hide

References

« Hide 'large scale' references
[1]"Secondary thrombin-binding site, glycosaminoglycan binding domain and reactive center region of leuserpin-2 are strongly conserved in mammalian species."
Westrup D., Ragg H.
Biochim. Biophys. Acta 1217:93-96(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X74550 mRNA. Translation: CAA52644.1.
X74549 mRNA. Translation: CAA52643.1.
BC091145 mRNA. Translation: AAH91145.1.
PIRS41066.
RefSeqNP_077358.1. NM_024382.1.
XP_006248771.1. XM_006248709.1.
UniGeneRn.10553.

3D structure databases

ProteinModelPortalQ64268.
SMRQ64268. Positions 55-479.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSI04.019.

PTM databases

PhosphoSiteQ64268.

Proteomic databases

PaxDbQ64268.
PRIDEQ64268.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000031819; ENSRNOP00000031829; ENSRNOG00000001865.
GeneID79224.
KEGGrno:79224.

Organism-specific databases

CTD3053.
RGD619854. Serpind1.

Phylogenomic databases

eggNOGCOG4826.
GeneTreeENSGT00750000117478.
HOGENOMHOG000294159.
HOVERGENHBG101242.
InParanoidQ64268.
KOK03912.
OMAADHELEC.
OrthoDBEOG7327PB.
PhylomeDBQ64268.
TreeFamTF343094.

Gene expression databases

GenevestigatorQ64268.

Family and domain databases

InterProIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 1 hit.
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio614652.
PROQ64268.

Entry information

Entry nameHEP2_RAT
AccessionPrimary (citable) accession number: Q64268
Secondary accession number(s): Q5BKA6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families