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Protein

Heparin cofactor 2

Gene

Serpind1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT) (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei443 – 4442Reactive bondBy similarity

GO - Molecular functioni

  • heparin binding Source: UniProtKB-KW
  • serine-type endopeptidase inhibitor activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Biological processi

Blood coagulation, Hemostasis

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiREACT_323052. Common Pathway of Fibrin Clot Formation.
REACT_343460. Intrinsic Pathway of Fibrin Clot Formation.

Protein family/group databases

MEROPSiI04.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparin cofactor 2
Alternative name(s):
Heparin cofactor II
Short name:
HC-II
Protease inhibitor leuserpin-2
Serpin D1
Gene namesi
Name:Serpind1
Synonyms:Hcf2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi619854. Serpind1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 479456Heparin cofactor 2PRO_0000032497Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi31 – 311N-linked (GlcNAc...)Sequence Analysis
Modified residuei61 – 611SulfotyrosineBy similarity
Modified residuei74 – 741SulfotyrosineBy similarity
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Different composition of the N-linked oligosaccharides appears to yield a 68-kDa and a 72-kDa form.By similarity

Keywords - PTMi

Glycoprotein, Sulfation

Proteomic databases

PaxDbiQ64268.
PRIDEiQ64268.

PTM databases

PhosphoSiteiQ64268.

Expressioni

Gene expression databases

GenevisibleiQ64268. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031829.

Structurei

3D structure databases

ProteinModelPortaliQ64268.
SMRiQ64268. Positions 55-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati55 – 65111Add
BLAST
Repeati69 – 79112Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni55 – 79252 X 11 AA approximate repeats, Asp/Glu-rich (acidic) (hirudin-like)Add
BLAST
Regioni172 – 19221Glycosaminoglycan-binding siteBy similarityAdd
BLAST

Domaini

The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition.By similarity

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4826.
GeneTreeiENSGT00760000118839.
HOGENOMiHOG000294159.
HOVERGENiHBG101242.
InParanoidiQ64268.
KOiK03912.
OMAiSDQRITI.
OrthoDBiEOG7327PB.
PhylomeDBiQ64268.
TreeFamiTF343094.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64268-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHPAYTLLL SLIMSMCAGS KGLAEQLTKE NLTVSLLPPN FHKENTVTND
60 70 80 90 100
WIPEGEEDDD YLDLEKLLSE DDDYIYVVDA VSPTDSESSA GNILQLFQGK
110 120 130 140 150
SRIQRLNILN AKFAFNLYRV LKDQATSSDN IFIAPVGIST AMGMISLGLR
160 170 180 190 200
GETHEEVHSV LHFKDFVNAS SKYEVTTIHN LFRKLTHRLF RRNFGYTLQS
210 220 230 240 250
VNDLYIQKQF PIREDFKAAM REFYFAEAQE ADFSDPAFIS KANSHILKLT
260 270 280 290 300
KGLIKEALEN TDSATQMMIL NCIYFKGAWM NKFPVEMTHN HNFRLNEREV
310 320 330 340 350
VKVSMMQTKG NFLAANDQEL DCDILQLEYV GGISMLIVIP RKLSGMKTLE
360 370 380 390 400
AQLTPQVVER WQKSMTNRTR EVLLPKFKLE KNYNLVEVLK SMGITKLFNK
410 420 430 440 450
NGNMSGISDQ RIIIDLFKHQ STITVNEEGT QAAAVTTVGF MPLSTQVRFT
460 470
VDRPFLFLVY EHRTSCLLFM GRVANPAKS
Length:479
Mass (Da):54,552
Last modified:November 1, 1997 - v1
Checksum:i4F736C2F8459E514
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74550 mRNA. Translation: CAA52644.1.
X74549 mRNA. Translation: CAA52643.1.
BC091145 mRNA. Translation: AAH91145.1.
PIRiS41066.
RefSeqiNP_077358.1. NM_024382.1.
XP_006248771.1. XM_006248709.2.
UniGeneiRn.10553.

Genome annotation databases

EnsembliENSRNOT00000031819; ENSRNOP00000031829; ENSRNOG00000001865.
GeneIDi79224.
KEGGirno:79224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74550 mRNA. Translation: CAA52644.1.
X74549 mRNA. Translation: CAA52643.1.
BC091145 mRNA. Translation: AAH91145.1.
PIRiS41066.
RefSeqiNP_077358.1. NM_024382.1.
XP_006248771.1. XM_006248709.2.
UniGeneiRn.10553.

3D structure databases

ProteinModelPortaliQ64268.
SMRiQ64268. Positions 55-479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031829.

Protein family/group databases

MEROPSiI04.019.

PTM databases

PhosphoSiteiQ64268.

Proteomic databases

PaxDbiQ64268.
PRIDEiQ64268.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000031819; ENSRNOP00000031829; ENSRNOG00000001865.
GeneIDi79224.
KEGGirno:79224.

Organism-specific databases

CTDi3053.
RGDi619854. Serpind1.

Phylogenomic databases

eggNOGiCOG4826.
GeneTreeiENSGT00760000118839.
HOGENOMiHOG000294159.
HOVERGENiHBG101242.
InParanoidiQ64268.
KOiK03912.
OMAiSDQRITI.
OrthoDBiEOG7327PB.
PhylomeDBiQ64268.
TreeFamiTF343094.

Enzyme and pathway databases

ReactomeiREACT_323052. Common Pathway of Fibrin Clot Formation.
REACT_343460. Intrinsic Pathway of Fibrin Clot Formation.

Miscellaneous databases

NextBioi614652.
PROiQ64268.

Gene expression databases

GenevisibleiQ64268. RN.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Secondary thrombin-binding site, glycosaminoglycan binding domain and reactive center region of leuserpin-2 are strongly conserved in mammalian species."
    Westrup D., Ragg H.
    Biochim. Biophys. Acta 1217:93-96(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiHEP2_RAT
AccessioniPrimary (citable) accession number: Q64268
Secondary accession number(s): Q5BKA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.