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Q64267

- XPA_MOUSE

UniProt

Q64267 - XPA_MOUSE

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Protein

DNA repair protein complementing XP-A cells homolog

Gene

Xpa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri104 – 12825Add
BLAST

GO - Molecular functioni

  1. damaged DNA binding Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA repair Source: MGI
  2. intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  3. multicellular organism growth Source: MGI
  4. nucleotide-excision repair Source: MGI
  5. response to oxidative stress Source: MGI
  6. response to toxic substance Source: MGI
  7. response to UV Source: MGI
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_240507. Dual incision reaction in GG-NER.
REACT_252106. Formation of incision complex in GG-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein complementing XP-A cells homolog
Alternative name(s):
Xeroderma pigmentosum group A-complementing protein homolog
Gene namesi
Name:Xpa
Synonyms:Xpac
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:99135. Xpa.

Subcellular locationi

GO - Cellular componenti

  1. DNA replication factor A complex Source: Ensembl
  2. Golgi apparatus Source: Ensembl
  3. intercellular bridge Source: Ensembl
  4. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Deficient mice cannot repair UV-induced DNA damage and easily develop skin cancers by UV irradiation. They develop stronger longer-lasting acute inflammation, they show a more severe UV-induced damage of keratinocytes and Langerhans cells as well as the enhancement of local and systemic immunosuppression. PGE2 and COX2 expression is greatly increased after UVB irradiation, this causes the enhancement of inflammation and immunosuppression. Natural killer cell activity is also significantly decreased.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 272271DNA repair protein complementing XP-A cells homologPRO_0000208649Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei195 – 1951PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated by HERC2 leading to degradation by the proteasome.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ64267.
PRIDEiQ64267.

PTM databases

PhosphoSiteiQ64267.

Expressioni

Inductioni

Exhibits a circadian pattern with zenith at around 5 pm and nadir at around 5 am in liver but not in testis, this oscillation is dependent on the circadian clock and on HERC2 regulation.1 Publication

Gene expression databases

BgeeiQ64267.
CleanExiMM_XPA.
ExpressionAtlasiQ64267. baseline and differential.
GenevestigatoriQ64267.

Interactioni

Subunit structurei

Interacts with GPN1. Interacts with RPA1 and RPA2; the interaction is direct and associates XPA with the RPA complex. Interacts (via N-terminus) with CEP164 upon UV irradiation. Interacts with HERC2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi204604. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ64267.
SMRiQ64267. Positions 97-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 9693Interaction with CEP164 and required for UV resistanceBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi28 – 4922Nuclear localization signalSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi78 – 825Poly-Glu

Sequence similaritiesi

Belongs to the XPA family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri104 – 12825Add
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5145.
GeneTreeiENSGT00390000002721.
HOGENOMiHOG000045820.
HOVERGENiHBG009053.
InParanoidiQ64267.
KOiK10847.
OMAiVAHQHEY.
TreeFamiTF101241.

Family and domain databases

Gene3Di3.90.530.10. 1 hit.
InterProiIPR009061. DNA-bd_dom_put.
IPR000465. XPA.
IPR022656. XPA_C.
IPR022658. XPA_CS.
IPR022652. Znf_XPA_CS.
[Graphical view]
PANTHERiPTHR10142. PTHR10142. 1 hit.
PfamiPF05181. XPA_C. 1 hit.
PF01286. XPA_N. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
TIGRFAMsiTIGR00598. rad14. 1 hit.
PROSITEiPS00752. XPA_1. 1 hit.
PS00753. XPA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q64267-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATAEEKQTS PEPVAADEPA QLPAAVRASV ERKRQRALML RQARLAARPY
60 70 80 90 100
PAAAATGGVA SVKAAPKMID TKGGFILEEE EEKHEIGNIV HEPGPVMEFD
110 120 130 140 150
YTICEECGKE FMDSYLMNHF DLPTCDSCRD ADDKHKLITK TEAKQEYLLK
160 170 180 190 200
DCDLEKREPA LRFLVKKNPR HSQWGDMKLY LKLQVVKRAL EVWGSQEALE
210 220 230 240 250
DAKEVRQENR EKMKQKKFDK KVKELRRAIR SSVWKRETTT HQHKYGPEEN
260 270
LEDDMYRKTC TLCGHELTYE KM
Length:272
Mass (Da):31,398
Last modified:April 14, 2009 - v2
Checksum:i9061C9528F9E63CB
GO
Isoform 2 (identifier: Q64267-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     224-272: ELRRAIRSSV...CGHELTYEKM → GKWPDFLSRR...TSLIKAIVFM

Show »
Length:273
Mass (Da):30,957
Checksum:i29A9CFEFAAC2E79F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti244 – 2441K → E in CAA52392. (PubMed:8127648)Curated
Sequence conflicti244 – 2441K → E in CAA52393. (PubMed:8127648)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei224 – 27249ELRRA…TYEKM → GKWPDFLSRRSQSTVSVGKE RGVNAGLRALVYEAWVNPHG TSLIKAIVFM in isoform 2. 1 PublicationVSP_036906Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74345
, X74346, X74347, X74348, X74349, X74350 Genomic DNA. Translation: CAA52392.1.
X74351 mRNA. Translation: CAA52393.1.
AK008962 mRNA. Translation: BAB25992.1.
AL806523 Genomic DNA. Translation: CAM23949.1.
AL806523 Genomic DNA. Translation: CAM23950.1.
CCDSiCCDS18146.1. [Q64267-1]
PIRiS41498.
RefSeqiNP_035858.2. NM_011728.2. [Q64267-1]
UniGeneiMm.247036.

Genome annotation databases

EnsembliENSMUST00000030013; ENSMUSP00000030013; ENSMUSG00000028329. [Q64267-1]
ENSMUST00000142380; ENSMUSP00000121850; ENSMUSG00000028329. [Q64267-2]
GeneIDi22590.
KEGGimmu:22590.
UCSCiuc008stm.2. mouse. [Q64267-1]
uc008stn.1. mouse. [Q64267-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74345
, X74346 , X74347 , X74348 , X74349 , X74350 Genomic DNA. Translation: CAA52392.1 .
X74351 mRNA. Translation: CAA52393.1 .
AK008962 mRNA. Translation: BAB25992.1 .
AL806523 Genomic DNA. Translation: CAM23949.1 .
AL806523 Genomic DNA. Translation: CAM23950.1 .
CCDSi CCDS18146.1. [Q64267-1 ]
PIRi S41498.
RefSeqi NP_035858.2. NM_011728.2. [Q64267-1 ]
UniGenei Mm.247036.

3D structure databases

ProteinModelPortali Q64267.
SMRi Q64267. Positions 97-209.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204604. 1 interaction.

PTM databases

PhosphoSitei Q64267.

Proteomic databases

MaxQBi Q64267.
PRIDEi Q64267.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030013 ; ENSMUSP00000030013 ; ENSMUSG00000028329 . [Q64267-1 ]
ENSMUST00000142380 ; ENSMUSP00000121850 ; ENSMUSG00000028329 . [Q64267-2 ]
GeneIDi 22590.
KEGGi mmu:22590.
UCSCi uc008stm.2. mouse. [Q64267-1 ]
uc008stn.1. mouse. [Q64267-2 ]

Organism-specific databases

CTDi 7507.
MGIi MGI:99135. Xpa.

Phylogenomic databases

eggNOGi COG5145.
GeneTreei ENSGT00390000002721.
HOGENOMi HOG000045820.
HOVERGENi HBG009053.
InParanoidi Q64267.
KOi K10847.
OMAi VAHQHEY.
TreeFami TF101241.

Enzyme and pathway databases

Reactomei REACT_240507. Dual incision reaction in GG-NER.
REACT_252106. Formation of incision complex in GG-NER.

Miscellaneous databases

NextBioi 302929.
PROi Q64267.
SOURCEi Search...

Gene expression databases

Bgeei Q64267.
CleanExi MM_XPA.
ExpressionAtlasi Q64267. baseline and differential.
Genevestigatori Q64267.

Family and domain databases

Gene3Di 3.90.530.10. 1 hit.
InterProi IPR009061. DNA-bd_dom_put.
IPR000465. XPA.
IPR022656. XPA_C.
IPR022658. XPA_CS.
IPR022652. Znf_XPA_CS.
[Graphical view ]
PANTHERi PTHR10142. PTHR10142. 1 hit.
Pfami PF05181. XPA_C. 1 hit.
PF01286. XPA_N. 1 hit.
[Graphical view ]
SUPFAMi SSF46955. SSF46955. 1 hit.
TIGRFAMsi TIGR00598. rad14. 1 hit.
PROSITEi PS00752. XPA_1. 1 hit.
PS00753. XPA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
    Strain: 129/Ola.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Stomach.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "Photobiologic and photoimmunologic characteristics of XPA gene-deficient mice."
    Horio T., Miyauchi-Hashimoto H., Kuwamoto K., Horiki S., Okamoto H., Tanaka K.
    J. Investig. Dermatol. Symp. Proc. 6:58-63(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: Hos/HR-1.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  6. "Circadian control of XPA and excision repair of cisplatin-DNA damage by cryptochrome and HERC2 ubiquitin ligase."
    Kang T.H., Lindsey-Boltz L.A., Reardon J.T., Sancar A.
    Proc. Natl. Acad. Sci. U.S.A. 107:4890-4895(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, MISCELLANEOUS.
    Strain: C57BL/6J.

Entry informationi

Entry nameiXPA_MOUSE
AccessioniPrimary (citable) accession number: Q64267
Secondary accession number(s): A2ALZ7, Q9CVA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 14, 2009
Last modified: November 26, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Plays an essential role in the repair of cisplatin induced damage by nucleotide excision repair. Cisplatin is one of the most commnly used anticancer drugs.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3