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Q64267

- XPA_MOUSE

UniProt

Q64267 - XPA_MOUSE

Protein

DNA repair protein complementing XP-A cells homolog

Gene

Xpa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (14 Apr 2009)
      Previous versions | rss
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    Functioni

    Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri104 – 12825Add
    BLAST

    GO - Molecular functioni

    1. damaged DNA binding Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA repair Source: MGI
    2. intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
    3. multicellular organism growth Source: MGI
    4. nucleotide-excision repair Source: MGI
    5. response to oxidative stress Source: MGI
    6. response to toxic substance Source: MGI
    7. response to UV Source: MGI

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair protein complementing XP-A cells homolog
    Alternative name(s):
    Xeroderma pigmentosum group A-complementing protein homolog
    Gene namesi
    Name:Xpa
    Synonyms:Xpac
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:99135. Xpa.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Deficient mice cannot repair UV-induced DNA damage and easily develop skin cancers by UV irradiation. They develop stronger longer-lasting acute inflammation, they show a more severe UV-induced damage of keratinocytes and Langerhans cells as well as the enhancement of local and systemic immunosuppression. PGE2 and COX2 expression is greatly increased after UVB irradiation, this causes the enhancement of inflammation and immunosuppression. Natural killer cell activity is also significantly decreased.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 272271DNA repair protein complementing XP-A cells homologPRO_0000208649Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei195 – 1951PhosphoserineBy similarity

    Post-translational modificationi

    Ubiquitinated by HERC2 leading to degradation by the proteasome.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ64267.

    PTM databases

    PhosphoSiteiQ64267.

    Expressioni

    Inductioni

    Exhibits a circadian pattern with zenith at around 5 pm and nadir at around 5 am in liver but not in testis, this oscillation is dependent on the circadian clock and on HERC2 regulation.1 Publication

    Gene expression databases

    ArrayExpressiQ64267.
    BgeeiQ64267.
    CleanExiMM_XPA.
    GenevestigatoriQ64267.

    Interactioni

    Subunit structurei

    Interacts with GPN1. Interacts with RPA1 and RPA2; the interaction is direct and associates XPA with the RPA complex. Interacts (via N-terminus) with CEP164 upon UV irradiation. Interacts with HERC2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi204604. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64267.
    SMRiQ64267. Positions 97-209.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni4 – 9693Interaction with CEP164 and required for UV resistanceBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi28 – 4922Nuclear localization signalSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi78 – 825Poly-Glu

    Sequence similaritiesi

    Belongs to the XPA family.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri104 – 12825Add
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5145.
    GeneTreeiENSGT00390000002721.
    HOGENOMiHOG000045820.
    HOVERGENiHBG009053.
    KOiK10847.
    OMAiVAHQHEY.
    TreeFamiTF101241.

    Family and domain databases

    Gene3Di3.90.530.10. 1 hit.
    InterProiIPR009061. DNA-bd_dom_put.
    IPR000465. XPA.
    IPR022656. XPA_C.
    IPR022658. XPA_CS.
    IPR022652. Znf_XPA_CS.
    [Graphical view]
    PANTHERiPTHR10142. PTHR10142. 1 hit.
    PfamiPF05181. XPA_C. 1 hit.
    PF01286. XPA_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF46955. SSF46955. 1 hit.
    TIGRFAMsiTIGR00598. rad14. 1 hit.
    PROSITEiPS00752. XPA_1. 1 hit.
    PS00753. XPA_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q64267-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATAEEKQTS PEPVAADEPA QLPAAVRASV ERKRQRALML RQARLAARPY    50
    PAAAATGGVA SVKAAPKMID TKGGFILEEE EEKHEIGNIV HEPGPVMEFD 100
    YTICEECGKE FMDSYLMNHF DLPTCDSCRD ADDKHKLITK TEAKQEYLLK 150
    DCDLEKREPA LRFLVKKNPR HSQWGDMKLY LKLQVVKRAL EVWGSQEALE 200
    DAKEVRQENR EKMKQKKFDK KVKELRRAIR SSVWKRETTT HQHKYGPEEN 250
    LEDDMYRKTC TLCGHELTYE KM 272
    Length:272
    Mass (Da):31,398
    Last modified:April 14, 2009 - v2
    Checksum:i9061C9528F9E63CB
    GO
    Isoform 2 (identifier: Q64267-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         224-272: ELRRAIRSSV...CGHELTYEKM → GKWPDFLSRR...TSLIKAIVFM

    Show »
    Length:273
    Mass (Da):30,957
    Checksum:i29A9CFEFAAC2E79F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti244 – 2441K → E in CAA52392. (PubMed:8127648)Curated
    Sequence conflicti244 – 2441K → E in CAA52393. (PubMed:8127648)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei224 – 27249ELRRA…TYEKM → GKWPDFLSRRSQSTVSVGKE RGVNAGLRALVYEAWVNPHG TSLIKAIVFM in isoform 2. 1 PublicationVSP_036906Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74345
    , X74346, X74347, X74348, X74349, X74350 Genomic DNA. Translation: CAA52392.1.
    X74351 mRNA. Translation: CAA52393.1.
    AK008962 mRNA. Translation: BAB25992.1.
    AL806523 Genomic DNA. Translation: CAM23949.1.
    AL806523 Genomic DNA. Translation: CAM23950.1.
    CCDSiCCDS18146.1. [Q64267-1]
    PIRiS41498.
    RefSeqiNP_035858.2. NM_011728.2. [Q64267-1]
    UniGeneiMm.247036.

    Genome annotation databases

    EnsembliENSMUST00000030013; ENSMUSP00000030013; ENSMUSG00000028329. [Q64267-1]
    ENSMUST00000142380; ENSMUSP00000121850; ENSMUSG00000028329. [Q64267-2]
    GeneIDi22590.
    KEGGimmu:22590.
    UCSCiuc008stm.2. mouse. [Q64267-1]
    uc008stn.1. mouse. [Q64267-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74345
    , X74346 , X74347 , X74348 , X74349 , X74350 Genomic DNA. Translation: CAA52392.1 .
    X74351 mRNA. Translation: CAA52393.1 .
    AK008962 mRNA. Translation: BAB25992.1 .
    AL806523 Genomic DNA. Translation: CAM23949.1 .
    AL806523 Genomic DNA. Translation: CAM23950.1 .
    CCDSi CCDS18146.1. [Q64267-1 ]
    PIRi S41498.
    RefSeqi NP_035858.2. NM_011728.2. [Q64267-1 ]
    UniGenei Mm.247036.

    3D structure databases

    ProteinModelPortali Q64267.
    SMRi Q64267. Positions 97-209.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204604. 1 interaction.

    PTM databases

    PhosphoSitei Q64267.

    Proteomic databases

    PRIDEi Q64267.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030013 ; ENSMUSP00000030013 ; ENSMUSG00000028329 . [Q64267-1 ]
    ENSMUST00000142380 ; ENSMUSP00000121850 ; ENSMUSG00000028329 . [Q64267-2 ]
    GeneIDi 22590.
    KEGGi mmu:22590.
    UCSCi uc008stm.2. mouse. [Q64267-1 ]
    uc008stn.1. mouse. [Q64267-2 ]

    Organism-specific databases

    CTDi 7507.
    MGIi MGI:99135. Xpa.

    Phylogenomic databases

    eggNOGi COG5145.
    GeneTreei ENSGT00390000002721.
    HOGENOMi HOG000045820.
    HOVERGENi HBG009053.
    KOi K10847.
    OMAi VAHQHEY.
    TreeFami TF101241.

    Miscellaneous databases

    NextBioi 302929.
    PROi Q64267.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64267.
    Bgeei Q64267.
    CleanExi MM_XPA.
    Genevestigatori Q64267.

    Family and domain databases

    Gene3Di 3.90.530.10. 1 hit.
    InterProi IPR009061. DNA-bd_dom_put.
    IPR000465. XPA.
    IPR022656. XPA_C.
    IPR022658. XPA_CS.
    IPR022652. Znf_XPA_CS.
    [Graphical view ]
    PANTHERi PTHR10142. PTHR10142. 1 hit.
    Pfami PF05181. XPA_C. 1 hit.
    PF01286. XPA_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46955. SSF46955. 1 hit.
    TIGRFAMsi TIGR00598. rad14. 1 hit.
    PROSITEi PS00752. XPA_1. 1 hit.
    PS00753. XPA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
      Strain: 129/Ola.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Stomach.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "Photobiologic and photoimmunologic characteristics of XPA gene-deficient mice."
      Horio T., Miyauchi-Hashimoto H., Kuwamoto K., Horiki S., Okamoto H., Tanaka K.
      J. Investig. Dermatol. Symp. Proc. 6:58-63(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
      Strain: Hos/HR-1.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    6. "Circadian control of XPA and excision repair of cisplatin-DNA damage by cryptochrome and HERC2 ubiquitin ligase."
      Kang T.H., Lindsey-Boltz L.A., Reardon J.T., Sancar A.
      Proc. Natl. Acad. Sci. U.S.A. 107:4890-4895(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, MISCELLANEOUS.
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiXPA_MOUSE
    AccessioniPrimary (citable) accession number: Q64267
    Secondary accession number(s): A2ALZ7, Q9CVA0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: April 14, 2009
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Plays an essential role in the repair of cisplatin induced damage by nucleotide excision repair. Cisplatin is one of the most commnly used anticancer drugs.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3