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Q64267 (XPA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA repair protein complementing XP-A cells homolog
Alternative name(s):
Xeroderma pigmentosum group A-complementing protein homolog
Gene names
Name:Xpa
Synonyms:Xpac
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation By similarity.

Subunit structure

Interacts with GPN1. Interacts with RPA1 and RPA2; the interaction is direct and associates XPA with the RPA complex. Interacts (via N-terminus) with CEP164 upon UV irradiation. Interacts with HERC2 By similarity.

Subcellular location

Nucleus.

Induction

Exhibits a circadian pattern with zenith at around 5 pm and nadir at around 5 am in liver but not in testis, this oscillation is dependent on the circadian clock and on HERC2 regulation. Ref.6

Post-translational modification

Ubiquitinated by HERC2 leading to degradation by the proteasome By similarity.

Disruption phenotype

Deficient mice cannot repair UV-induced DNA damage and easily develop skin cancers by UV irradiation. They develop stronger longer-lasting acute inflammation, they show a more severe UV-induced damage of keratinocytes and Langerhans cells as well as the enhancement of local and systemic immunosuppression. PGE2 and COX2 expression is greatly increased after UVB irradiation, this causes the enhancement of inflammation and immunosuppression. Natural killer cell activity is also significantly decreased. Ref.4

Miscellaneous

Plays an essential role in the repair of cisplatin induced damage by nucleotide excision repair. Cisplatin is one of the most commnly used anticancer drugs.

Sequence similarities

Belongs to the XPA family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q64267-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q64267-2)

The sequence of this isoform differs from the canonical sequence as follows:
     224-272: ELRRAIRSSV...CGHELTYEKM → GKWPDFLSRR...TSLIKAIVFM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 272271DNA repair protein complementing XP-A cells homolog
PRO_0000208649

Regions

Zinc finger104 – 12825
Region4 – 9693Interaction with CEP164 and required for UV resistance By similarity
Motif28 – 4922Nuclear localization signal Potential
Compositional bias78 – 825Poly-Glu

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue1951Phosphoserine By similarity

Natural variations

Alternative sequence224 – 27249ELRRA…TYEKM → GKWPDFLSRRSQSTVSVGKE RGVNAGLRALVYEAWVNPHG TSLIKAIVFM in isoform 2.
VSP_036906

Experimental info

Sequence conflict2441K → E in CAA52392. Ref.1
Sequence conflict2441K → E in CAA52393. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 14, 2009. Version 2.
Checksum: 9061C9528F9E63CB

FASTA27231,398
        10         20         30         40         50         60 
MATAEEKQTS PEPVAADEPA QLPAAVRASV ERKRQRALML RQARLAARPY PAAAATGGVA 

        70         80         90        100        110        120 
SVKAAPKMID TKGGFILEEE EEKHEIGNIV HEPGPVMEFD YTICEECGKE FMDSYLMNHF 

       130        140        150        160        170        180 
DLPTCDSCRD ADDKHKLITK TEAKQEYLLK DCDLEKREPA LRFLVKKNPR HSQWGDMKLY 

       190        200        210        220        230        240 
LKLQVVKRAL EVWGSQEALE DAKEVRQENR EKMKQKKFDK KVKELRRAIR SSVWKRETTT 

       250        260        270 
HQHKYGPEEN LEDDMYRKTC TLCGHELTYE KM 

« Hide

Isoform 2 [UniParc].

Checksum: 29A9CFEFAAC2E79F
Show »

FASTA27330,957

References

« Hide 'large scale' references
[1]"Cloning and characterization of the mouse XPAC gene."
van Oostrom C.T.M., de Vries A., Verbeek S.J., van Kreijl C.F., van Steeg H.
Nucleic Acids Res. 22:11-14(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
Strain: 129/Ola.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Stomach.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"Photobiologic and photoimmunologic characteristics of XPA gene-deficient mice."
Horio T., Miyauchi-Hashimoto H., Kuwamoto K., Horiki S., Okamoto H., Tanaka K.
J. Investig. Dermatol. Symp. Proc. 6:58-63(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
Strain: Hos/HR-1.
[5]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[6]"Circadian control of XPA and excision repair of cisplatin-DNA damage by cryptochrome and HERC2 ubiquitin ligase."
Kang T.H., Lindsey-Boltz L.A., Reardon J.T., Sancar A.
Proc. Natl. Acad. Sci. U.S.A. 107:4890-4895(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, MISCELLANEOUS.
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X74345 expand/collapse EMBL AC list , X74346, X74347, X74348, X74349, X74350 Genomic DNA. Translation: CAA52392.1.
X74351 mRNA. Translation: CAA52393.1.
AK008962 mRNA. Translation: BAB25992.1.
AL806523 Genomic DNA. Translation: CAM23949.1.
AL806523 Genomic DNA. Translation: CAM23950.1.
CCDSCCDS18146.1. [Q64267-1]
PIRS41498.
RefSeqNP_035858.2. NM_011728.2. [Q64267-1]
UniGeneMm.247036.

3D structure databases

ProteinModelPortalQ64267.
SMRQ64267. Positions 97-209.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204604. 1 interaction.

PTM databases

PhosphoSiteQ64267.

Proteomic databases

PRIDEQ64267.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030013; ENSMUSP00000030013; ENSMUSG00000028329. [Q64267-1]
ENSMUST00000142380; ENSMUSP00000121850; ENSMUSG00000028329. [Q64267-2]
GeneID22590.
KEGGmmu:22590.
UCSCuc008stm.2. mouse. [Q64267-1]
uc008stn.1. mouse. [Q64267-2]

Organism-specific databases

CTD7507.
MGIMGI:99135. Xpa.

Phylogenomic databases

eggNOGCOG5145.
GeneTreeENSGT00390000002721.
HOGENOMHOG000045820.
HOVERGENHBG009053.
KOK10847.
OMAVAHQHEY.
TreeFamTF101241.

Gene expression databases

ArrayExpressQ64267.
BgeeQ64267.
CleanExMM_XPA.
GenevestigatorQ64267.

Family and domain databases

Gene3D3.90.530.10. 1 hit.
InterProIPR009061. DNA-bd_dom_put.
IPR000465. XPA.
IPR022656. XPA_C.
IPR022658. XPA_CS.
IPR022652. Znf_XPA_CS.
[Graphical view]
PANTHERPTHR10142. PTHR10142. 1 hit.
PfamPF05181. XPA_C. 1 hit.
PF01286. XPA_N. 1 hit.
[Graphical view]
SUPFAMSSF46955. SSF46955. 1 hit.
TIGRFAMsTIGR00598. rad14. 1 hit.
PROSITEPS00752. XPA_1. 1 hit.
PS00753. XPA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302929.
PROQ64267.
SOURCESearch...

Entry information

Entry nameXPA_MOUSE
AccessionPrimary (citable) accession number: Q64267
Secondary accession number(s): A2ALZ7, Q9CVA0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 14, 2009
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot