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Protein

5-hydroxytryptamine receptor 1A

Gene

Htr1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.6 Publications

GO - Molecular functioni

  • G-protein coupled serotonin receptor activity Source: UniProtKB
  • neurotransmitter receptor activity Source: GO_Central
  • serotonin binding Source: GO_Central

GO - Biological processi

  • adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: UniProtKB
  • behavioral fear response Source: UniProtKB
  • cell proliferation Source: InterPro
  • chemical synaptic transmission Source: GO_Central
  • exploration behavior Source: UniProtKB
  • G-protein coupled receptor signaling pathway Source: MGI
  • regulation of behavior Source: InterPro
  • regulation of dopamine metabolic process Source: UniProtKB
  • regulation of hormone secretion Source: InterPro
  • regulation of serotonin secretion Source: UniProtKB
  • serotonin metabolic process Source: UniProtKB
  • serotonin receptor signaling pathway Source: UniProtKB
  • vasoconstriction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Enzyme and pathway databases

ReactomeiR-MMU-390666. Serotonin receptors.
R-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 1A
Short name:
5-HT-1A
Short name:
5-HT1A
Alternative name(s):
Serotonin receptor 1A
Gene namesi
Name:Htr1a
Synonyms:Gpcr18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:96273. Htr1a.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36ExtracellularBy similarityAdd BLAST36
Transmembranei37 – 62Helical; Name=1By similarityAdd BLAST26
Topological domaini63 – 73CytoplasmicBy similarityAdd BLAST11
Transmembranei74 – 98Helical; Name=2By similarityAdd BLAST25
Topological domaini99 – 110ExtracellularBy similarityAdd BLAST12
Transmembranei111 – 132Helical; Name=3By similarityAdd BLAST22
Topological domaini133 – 152CytoplasmicBy similarityAdd BLAST20
Transmembranei153 – 178Helical; Name=4By similarityAdd BLAST26
Topological domaini179 – 191ExtracellularBy similarityAdd BLAST13
Transmembranei192 – 217Helical; Name=5By similarityAdd BLAST26
Topological domaini218 – 345CytoplasmicBy similarityAdd BLAST128
Transmembranei346 – 367Helical; Name=6By similarityAdd BLAST22
Topological domaini368 – 378ExtracellularBy similarityAdd BLAST11
Transmembranei379 – 403Helical; Name=7By similarityAdd BLAST25
Topological domaini404 – 421CytoplasmicBy similarityAdd BLAST18

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice display decreased exploratory behavior and increased fear-related behavior in anxiogenic environments. Mutant mice display altered monoamine metabolism in specific parts of the brain, especially in dorsal and medial raphe nuclei, thalamus and hypothalamus, leading to altered levels of 5-hydroxytryptamine, dopamine and their metabolites, as well as altered noradrenaline levels.3 Publications

Chemistry databases

ChEMBLiCHEMBL3737.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000689041 – 4215-hydroxytryptamine receptor 1AAdd BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi10N-linked (GlcNAc...)Sequence analysis1
Glycosylationi11N-linked (GlcNAc...)Sequence analysis1
Glycosylationi24N-linked (GlcNAc...)Sequence analysis1
Glycosylationi30N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi109 ↔ 187PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ64264.
PRIDEiQ64264.

PTM databases

iPTMnetiQ64264.
PhosphoSitePlusiQ64264.
SwissPalmiQ64264.

Expressioni

Tissue specificityi

Most abundantly expressed in midbrain, in dorsal raphe and hippocampus. Detected at lower levels in amygdala and brain cortex.1 Publication

Gene expression databases

BgeeiENSMUSG00000021721.
CleanExiMM_HTR1A.
GenevisibleiQ64264. MM.

Interactioni

Subunit structurei

Heterodimer; heterodimerizes with GPER1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022235.

Structurei

3D structure databases

ProteinModelPortaliQ64264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni112 – 121Agonist bindingBy similarity10
Regioni358 – 362Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi133 – 135DRY motif; important for ligand-induced conformation changesBy similarity3
Motifi396 – 400NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiQ64264.
KOiK04153.
OMAiYIHIRNE.
OrthoDBiEOG091G06VI.
TreeFamiTF316350.

Family and domain databases

InterProiIPR000610. 5HT1A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF20. PTHR24247:SF20. 2 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00512. 5HT1ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDMFSLGQGN NTTTSLEPFG TGGNDTGLSN VTFSYQVITS LLLGTLIFCA
60 70 80 90 100
VLGNACVVAA IALERSLQNV ANYLIGSLAV TDLMVSVLVL PMAALYQVLN
110 120 130 140 150
KWTLGQVTCD LFIALDVLCC TSSILHLCAI ALDRYWAITD PIDYVNKRTP
160 170 180 190 200
RRAAALISLT WLIGFLISIP PMLGWRTPED RSNPNECTIS KDHGYTIYST
210 220 230 240 250
FGAFYIPLLL MLVLYGRIFR AARFRIRKTV KKVEKKGAGT SFGTSSAPPP
260 270 280 290 300
KKSLNGQPGS GDCRRSAENR AVGTPCANGA VRQGEDDATL EVIEVHRVGN
310 320 330 340 350
SKGHLPLPSE SGATSYVPAC LERKNERTAE AKRKMALARE RKTVKTLGII
360 370 380 390 400
MGTFILCWLP FFIVALVLPF CESSCHMPEL LGAIINWLGY SNSLLNPVIY
410 420
AYFNKDFQNA FKKIIKCKFC R
Length:421
Mass (Da):46,176
Last modified:July 27, 2011 - v2
Checksum:i74F519DF7CC0C7AA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti177T → A in AAA81519 (PubMed:8254366).Curated1
Sequence conflicti242F → L in AAA16850 (PubMed:8288218).Curated1
Sequence conflicti247A → V in AAA16850 (PubMed:8288218).Curated1
Sequence conflicti263C → W in AAA16850 (PubMed:8288218).Curated1
Sequence conflicti304H → D in AAA81519 (PubMed:8254366).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti17 – 19EPF → DHL in strain: C3H/An. 3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39391 mRNA. Translation: AAA81519.1.
AK043668 mRNA. Translation: BAC31611.1.
AK049814 mRNA. Translation: BAC33931.1.
AK049884 mRNA. Translation: BAC33970.1.
AK140194 mRNA. Translation: BAE24273.1.
CH466568 Genomic DNA. Translation: EDL18479.1.
BC138669 mRNA. Translation: AAI38670.1.
BC138681 mRNA. Translation: AAI38682.1.
U33820 Genomic DNA. Translation: AAC52572.1.
L20339 mRNA. Translation: AAA16850.1.
CCDSiCCDS26756.1.
PIRiI49375.
RefSeqiNP_032334.2. NM_008308.4.
UniGeneiMm.4716.

Genome annotation databases

EnsembliENSMUST00000022235; ENSMUSP00000022235; ENSMUSG00000021721.
GeneIDi15550.
KEGGimmu:15550.
UCSCiuc007rtu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39391 mRNA. Translation: AAA81519.1.
AK043668 mRNA. Translation: BAC31611.1.
AK049814 mRNA. Translation: BAC33931.1.
AK049884 mRNA. Translation: BAC33970.1.
AK140194 mRNA. Translation: BAE24273.1.
CH466568 Genomic DNA. Translation: EDL18479.1.
BC138669 mRNA. Translation: AAI38670.1.
BC138681 mRNA. Translation: AAI38682.1.
U33820 Genomic DNA. Translation: AAC52572.1.
L20339 mRNA. Translation: AAA16850.1.
CCDSiCCDS26756.1.
PIRiI49375.
RefSeqiNP_032334.2. NM_008308.4.
UniGeneiMm.4716.

3D structure databases

ProteinModelPortaliQ64264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022235.

Chemistry databases

ChEMBLiCHEMBL3737.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ64264.
PhosphoSitePlusiQ64264.
SwissPalmiQ64264.

Proteomic databases

PaxDbiQ64264.
PRIDEiQ64264.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022235; ENSMUSP00000022235; ENSMUSG00000021721.
GeneIDi15550.
KEGGimmu:15550.
UCSCiuc007rtu.2. mouse.

Organism-specific databases

CTDi3350.
MGIiMGI:96273. Htr1a.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiQ64264.
KOiK04153.
OMAiYIHIRNE.
OrthoDBiEOG091G06VI.
TreeFamiTF316350.

Enzyme and pathway databases

ReactomeiR-MMU-390666. Serotonin receptors.
R-MMU-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiQ64264.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021721.
CleanExiMM_HTR1A.
GenevisibleiQ64264. MM.

Family and domain databases

InterProiIPR000610. 5HT1A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF20. PTHR24247:SF20. 2 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00512. 5HT1ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei5HT1A_MOUSE
AccessioniPrimary (citable) accession number: Q64264
Secondary accession number(s): Q60956, Q61617, Q8BGS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.