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Protein

Nuclear receptor subfamily 6 group A member 1

Gene

Nr6a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi72 – 147Nuclear receptorPROSITE-ProRule annotationAdd BLAST76
Zinc fingeri75 – 95NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri111 – 135NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Receptor

Keywords - Biological processi

Differentiation, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-383280. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 6 group A member 1
Alternative name(s):
Germ cell nuclear factor
Short name:
GCNF
Short name:
mGCNF
Retinoid receptor-related testis-specific receptor
Short name:
RTR
Gene namesi
Name:Nr6a1
Synonyms:Gcnf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1352459. Nr6a1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: GO_Central
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi246 – 247SY → GG: Abolishes interaction with RXRIP110. 1 Publication2
Mutagenesisi254 – 255LP → AA: Does not abolish interaction with RXRIP110. 1 Publication2
Mutagenesisi265 – 266SY → AA: Does not abolish interaction with RXRIP110. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000537421 – 495Nuclear receptor subfamily 6 group A member 1Add BLAST495

Proteomic databases

PaxDbiQ64249.
PRIDEiQ64249.

PTM databases

PhosphoSitePlusiQ64249.

Expressioni

Tissue specificityi

Expressed in the germ cells of both the adult testis and ovary, being most abundant in spermatids.2 Publications

Gene expression databases

BgeeiENSMUSG00000063972.
CleanExiMM_NR6A1.
ExpressionAtlasiQ64249. baseline and differential.
GenevisibleiQ64249. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with UIMC1.2 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi199868. 5 interactors.
STRINGi10090.ENSMUSP00000075624.

Structurei

3D structure databases

ProteinModelPortaliQ64249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni187 – 268Sufficient for interaction with UIMC1Add BLAST82

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri75 – 95NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri111 – 135NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00850000132285.
HOGENOMiHOG000113847.
HOVERGENiHBG052664.
InParanoidiQ64249.
KOiK08561.
OMAiHSCKSSL.
OrthoDBiEOG091G06MN.
PhylomeDBiQ64249.
TreeFamiTF350737.

Family and domain databases

Gene3Di1.10.565.10. 3 hits.
3.30.50.10. 1 hit.
InterProiIPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q64249-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERDERPPSG GGGGGGSAGF LEPPAALPPP PRNGFCQDEL AELDPGTNGE
60 70 80 90 100
TDSLTLGQGH IPVSVPDDRA EQRTCLICGD RATGLHYGII SCEGCKGFFK
110 120 130 140 150
RSICNKRVYR CSRDKNCVMS RKQRNRCQYC RLLKCLQMGM NRKAIREDGM
160 170 180 190 200
PGGRNKSIGP VQISEEEIER IMSGQEFEEE ANHWSNHGDS DHSSPGNRAS
210 220 230 240 250
ESNQPSPGST LSSSRSVELN GFMAFRDQYM GMSVPPHYQY IPHLFSYSGH
260 270 280 290 300
SPLLPPQARS LDPQSYSLIH QLMSAEDLEP LGTPMLIEDG YAVTQAELFA
310 320 330 340 350
LLCRLADELL FRQIAWIKKL PFFCELSIKD YTCLLSSTWQ ELILLSSLTV
360 370 380 390 400
YSKQIFGELA DVTAKYSPSD EELHRFSDEG MEVIERLIYL YHKFHQLKVS
410 420 430 440 450
NEEYACMKAI NFLNQDIRGL TSASQLEQLN KRYWYICQDF TEYKYTHQPN
460 470 480 490
RFPDLMMCLP EIRYIAGKMV NVPLEQLPLL FKVVLHSCKT STVKE
Length:495
Mass (Da):55,976
Last modified:November 1, 1996 - v1
Checksum:iECB0AB3E99E42661
GO
Isoform 2 (identifier: Q64249-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     48-62: Missing.
     63-63: V → I

Note: No experimental confirmation available.
Show »
Length:480
Mass (Da):54,469
Checksum:i17DA7AC5DB297A23
GO
Isoform 3 (identifier: Q64249-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: Missing.
     58-62: QGHIP → METWE

Note: No experimental confirmation available.
Show »
Length:438
Mass (Da):50,445
Checksum:i78B62010C39F7310
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0259281 – 57Missing in isoform 3. CuratedAdd BLAST57
Alternative sequenceiVSP_02592948 – 62Missing in isoform 2. CuratedAdd BLAST15
Alternative sequenceiVSP_02593058 – 62QGHIP → METWE in isoform 3. Curated5
Alternative sequenceiVSP_02593163V → I in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14666 mRNA. Translation: AAA92682.1.
U09563 mRNA. Translation: AAA64746.1.
AF254821
, AF254575, AF254815, AF254816, AF254817, AF254818, AF254819, AF254820 Genomic DNA. Translation: AAG24485.1.
AF390896 mRNA. Translation: AAM55193.1.
AF390900
, AF390897, AF390898, AF390899 Genomic DNA. Translation: AAM95878.1.
AL844842, AL935155 Genomic DNA. Translation: CAM21928.1.
AL844842, AL935155 Genomic DNA. Translation: CAM21929.1.
AL844842 Genomic DNA. Translation: CAM21933.1.
AL935155, AL844842 Genomic DNA. Translation: CAM21313.1.
AL935155, AL844842 Genomic DNA. Translation: CAM21314.1.
CCDSiCCDS16012.1. [Q64249-1]
CCDS50581.1. [Q64249-3]
PIRiA57053.
RefSeqiNP_001153020.1. NM_001159548.1. [Q64249-3]
NP_034394.1. NM_010264.4. [Q64249-1]
UniGeneiMm.439703.

Genome annotation databases

EnsembliENSMUST00000076275; ENSMUSP00000075624; ENSMUSG00000063972. [Q64249-1]
ENSMUST00000112877; ENSMUSP00000108498; ENSMUSG00000063972. [Q64249-1]
ENSMUST00000168098; ENSMUSP00000126009; ENSMUSG00000063972. [Q64249-3]
GeneIDi14536.
KEGGimmu:14536.
UCSCiuc012bus.1. mouse. [Q64249-3]
uc012buu.1. mouse. [Q64249-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14666 mRNA. Translation: AAA92682.1.
U09563 mRNA. Translation: AAA64746.1.
AF254821
, AF254575, AF254815, AF254816, AF254817, AF254818, AF254819, AF254820 Genomic DNA. Translation: AAG24485.1.
AF390896 mRNA. Translation: AAM55193.1.
AF390900
, AF390897, AF390898, AF390899 Genomic DNA. Translation: AAM95878.1.
AL844842, AL935155 Genomic DNA. Translation: CAM21928.1.
AL844842, AL935155 Genomic DNA. Translation: CAM21929.1.
AL844842 Genomic DNA. Translation: CAM21933.1.
AL935155, AL844842 Genomic DNA. Translation: CAM21313.1.
AL935155, AL844842 Genomic DNA. Translation: CAM21314.1.
CCDSiCCDS16012.1. [Q64249-1]
CCDS50581.1. [Q64249-3]
PIRiA57053.
RefSeqiNP_001153020.1. NM_001159548.1. [Q64249-3]
NP_034394.1. NM_010264.4. [Q64249-1]
UniGeneiMm.439703.

3D structure databases

ProteinModelPortaliQ64249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199868. 5 interactors.
STRINGi10090.ENSMUSP00000075624.

PTM databases

PhosphoSitePlusiQ64249.

Proteomic databases

PaxDbiQ64249.
PRIDEiQ64249.

Protocols and materials databases

DNASUi14536.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076275; ENSMUSP00000075624; ENSMUSG00000063972. [Q64249-1]
ENSMUST00000112877; ENSMUSP00000108498; ENSMUSG00000063972. [Q64249-1]
ENSMUST00000168098; ENSMUSP00000126009; ENSMUSG00000063972. [Q64249-3]
GeneIDi14536.
KEGGimmu:14536.
UCSCiuc012bus.1. mouse. [Q64249-3]
uc012buu.1. mouse. [Q64249-1]

Organism-specific databases

CTDi2649.
MGIiMGI:1352459. Nr6a1.

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00850000132285.
HOGENOMiHOG000113847.
HOVERGENiHBG052664.
InParanoidiQ64249.
KOiK08561.
OMAiHSCKSSL.
OrthoDBiEOG091G06MN.
PhylomeDBiQ64249.
TreeFamiTF350737.

Enzyme and pathway databases

ReactomeiR-MMU-383280. Nuclear Receptor transcription pathway.

Miscellaneous databases

ChiTaRSiNr6a1. mouse.
PROiQ64249.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000063972.
CleanExiMM_NR6A1.
ExpressionAtlasiQ64249. baseline and differential.
GenevisibleiQ64249. MM.

Family and domain databases

Gene3Di1.10.565.10. 3 hits.
3.30.50.10. 1 hit.
InterProiIPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNR6A1_MOUSE
AccessioniPrimary (citable) accession number: Q64249
Secondary accession number(s): A2AQL8, A2AQM1, Q687Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.