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Q64249

- NR6A1_MOUSE

UniProt

Q64249 - NR6A1_MOUSE

Protein

Nuclear receptor subfamily 6 group A member 1

Gene

Nr6a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi72 – 14776Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri75 – 9521NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri111 – 13525NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein binding Source: HGNC
    3. protein homodimerization activity Source: UniProtKB
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
    5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    6. sequence-specific DNA binding Source: UniProtKB
    7. steroid hormone receptor activity Source: InterPro
    8. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. gamete generation Source: UniProtKB
    3. multicellular organismal development Source: UniProtKB-KW
    4. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    5. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    6. spermatogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Receptor

    Keywords - Biological processi

    Differentiation, Spermatogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_220962. POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor subfamily 6 group A member 1
    Alternative name(s):
    Germ cell nuclear factor
    Short name:
    GCNF
    Short name:
    mGCNF
    Retinoid receptor-related testis-specific receptor
    Short name:
    RTR
    Gene namesi
    Name:Nr6a1
    Synonyms:Gcnf
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1352459. Nr6a1.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi246 – 2472SY → GG: Abolishes interaction with RXRIP110. 1 Publication
    Mutagenesisi254 – 2552LP → AA: Does not abolish interaction with RXRIP110. 1 Publication
    Mutagenesisi265 – 2662SY → AA: Does not abolish interaction with RXRIP110. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 495495Nuclear receptor subfamily 6 group A member 1PRO_0000053742Add
    BLAST

    Proteomic databases

    PRIDEiQ64249.

    PTM databases

    PhosphoSiteiQ64249.

    Expressioni

    Tissue specificityi

    Expressed in the germ cells of both the adult testis and ovary, being most abundant in spermatids.2 Publications

    Gene expression databases

    BgeeiQ64249.
    CleanExiMM_NR6A1.
    GenevestigatoriQ64249.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with UIMC1.2 Publications

    Protein-protein interaction databases

    BioGridi199868. 5 interactions.
    STRINGi10090.ENSMUSP00000108498.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64249.
    SMRiQ64249. Positions 72-484.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni187 – 26882Sufficient for interaction with UIMC1Add
    BLAST

    Sequence similaritiesi

    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri75 – 9521NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri111 – 13525NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG253308.
    GeneTreeiENSGT00750000117458.
    HOGENOMiHOG000113847.
    HOVERGENiHBG052664.
    InParanoidiQ64249.
    KOiK08561.
    OMAiHQLVSAE.
    OrthoDBiEOG78WKRG.
    PhylomeDBiQ64249.
    TreeFamiTF350737.

    Family and domain databases

    Gene3Di1.10.565.10. 3 hits.
    3.30.50.10. 1 hit.
    InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PRINTSiPR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 2 hits.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q64249-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MERDERPPSG GGGGGGSAGF LEPPAALPPP PRNGFCQDEL AELDPGTNGE    50
    TDSLTLGQGH IPVSVPDDRA EQRTCLICGD RATGLHYGII SCEGCKGFFK 100
    RSICNKRVYR CSRDKNCVMS RKQRNRCQYC RLLKCLQMGM NRKAIREDGM 150
    PGGRNKSIGP VQISEEEIER IMSGQEFEEE ANHWSNHGDS DHSSPGNRAS 200
    ESNQPSPGST LSSSRSVELN GFMAFRDQYM GMSVPPHYQY IPHLFSYSGH 250
    SPLLPPQARS LDPQSYSLIH QLMSAEDLEP LGTPMLIEDG YAVTQAELFA 300
    LLCRLADELL FRQIAWIKKL PFFCELSIKD YTCLLSSTWQ ELILLSSLTV 350
    YSKQIFGELA DVTAKYSPSD EELHRFSDEG MEVIERLIYL YHKFHQLKVS 400
    NEEYACMKAI NFLNQDIRGL TSASQLEQLN KRYWYICQDF TEYKYTHQPN 450
    RFPDLMMCLP EIRYIAGKMV NVPLEQLPLL FKVVLHSCKT STVKE 495
    Length:495
    Mass (Da):55,976
    Last modified:November 1, 1996 - v1
    Checksum:iECB0AB3E99E42661
    GO
    Isoform 2 (identifier: Q64249-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         48-62: Missing.
         63-63: V → I

    Note: No experimental confirmation available.

    Show »
    Length:480
    Mass (Da):54,469
    Checksum:i17DA7AC5DB297A23
    GO
    Isoform 3 (identifier: Q64249-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-57: Missing.
         58-62: QGHIP → METWE

    Note: No experimental confirmation available.

    Show »
    Length:438
    Mass (Da):50,445
    Checksum:i78B62010C39F7310
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5757Missing in isoform 3. CuratedVSP_025928Add
    BLAST
    Alternative sequencei48 – 6215Missing in isoform 2. CuratedVSP_025929Add
    BLAST
    Alternative sequencei58 – 625QGHIP → METWE in isoform 3. CuratedVSP_025930
    Alternative sequencei63 – 631V → I in isoform 2. CuratedVSP_025931

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14666 mRNA. Translation: AAA92682.1.
    U09563 mRNA. Translation: AAA64746.1.
    AF254821
    , AF254575, AF254815, AF254816, AF254817, AF254818, AF254819, AF254820 Genomic DNA. Translation: AAG24485.1.
    AF390896 mRNA. Translation: AAM55193.1.
    AF390900
    , AF390897, AF390898, AF390899 Genomic DNA. Translation: AAM95878.1.
    AL844842, AL935155 Genomic DNA. Translation: CAM21928.1.
    AL844842, AL935155 Genomic DNA. Translation: CAM21929.1.
    AL844842 Genomic DNA. Translation: CAM21933.1.
    AL935155, AL844842 Genomic DNA. Translation: CAM21313.1.
    AL935155, AL844842 Genomic DNA. Translation: CAM21314.1.
    CCDSiCCDS16012.1. [Q64249-1]
    CCDS50581.1. [Q64249-3]
    PIRiA57053.
    RefSeqiNP_001153020.1. NM_001159548.1. [Q64249-3]
    NP_034394.1. NM_010264.4. [Q64249-1]
    XP_006497771.1. XM_006497708.1. [Q64249-2]
    UniGeneiMm.439703.

    Genome annotation databases

    EnsembliENSMUST00000076275; ENSMUSP00000075624; ENSMUSG00000063972. [Q64249-1]
    ENSMUST00000112877; ENSMUSP00000108498; ENSMUSG00000063972. [Q64249-1]
    ENSMUST00000168098; ENSMUSP00000126009; ENSMUSG00000063972. [Q64249-3]
    GeneIDi14536.
    KEGGimmu:14536.
    UCSCiuc012bus.1. mouse. [Q64249-3]
    uc012buu.1. mouse. [Q64249-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14666 mRNA. Translation: AAA92682.1 .
    U09563 mRNA. Translation: AAA64746.1 .
    AF254821
    , AF254575 , AF254815 , AF254816 , AF254817 , AF254818 , AF254819 , AF254820 Genomic DNA. Translation: AAG24485.1 .
    AF390896 mRNA. Translation: AAM55193.1 .
    AF390900
    , AF390897 , AF390898 , AF390899 Genomic DNA. Translation: AAM95878.1 .
    AL844842 , AL935155 Genomic DNA. Translation: CAM21928.1 .
    AL844842 , AL935155 Genomic DNA. Translation: CAM21929.1 .
    AL844842 Genomic DNA. Translation: CAM21933.1 .
    AL935155 , AL844842 Genomic DNA. Translation: CAM21313.1 .
    AL935155 , AL844842 Genomic DNA. Translation: CAM21314.1 .
    CCDSi CCDS16012.1. [Q64249-1 ]
    CCDS50581.1. [Q64249-3 ]
    PIRi A57053.
    RefSeqi NP_001153020.1. NM_001159548.1. [Q64249-3 ]
    NP_034394.1. NM_010264.4. [Q64249-1 ]
    XP_006497771.1. XM_006497708.1. [Q64249-2 ]
    UniGenei Mm.439703.

    3D structure databases

    ProteinModelPortali Q64249.
    SMRi Q64249. Positions 72-484.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199868. 5 interactions.
    STRINGi 10090.ENSMUSP00000108498.

    PTM databases

    PhosphoSitei Q64249.

    Proteomic databases

    PRIDEi Q64249.

    Protocols and materials databases

    DNASUi 14536.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000076275 ; ENSMUSP00000075624 ; ENSMUSG00000063972 . [Q64249-1 ]
    ENSMUST00000112877 ; ENSMUSP00000108498 ; ENSMUSG00000063972 . [Q64249-1 ]
    ENSMUST00000168098 ; ENSMUSP00000126009 ; ENSMUSG00000063972 . [Q64249-3 ]
    GeneIDi 14536.
    KEGGi mmu:14536.
    UCSCi uc012bus.1. mouse. [Q64249-3 ]
    uc012buu.1. mouse. [Q64249-1 ]

    Organism-specific databases

    CTDi 2649.
    MGIi MGI:1352459. Nr6a1.

    Phylogenomic databases

    eggNOGi NOG253308.
    GeneTreei ENSGT00750000117458.
    HOGENOMi HOG000113847.
    HOVERGENi HBG052664.
    InParanoidi Q64249.
    KOi K08561.
    OMAi HQLVSAE.
    OrthoDBi EOG78WKRG.
    PhylomeDBi Q64249.
    TreeFami TF350737.

    Enzyme and pathway databases

    Reactomei REACT_220962. POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.

    Miscellaneous databases

    ChiTaRSi NR6A1. mouse.
    NextBioi 286194.
    PROi Q64249.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q64249.
    CleanExi MM_NR6A1.
    Genevestigatori Q64249.

    Family and domain databases

    Gene3Di 1.10.565.10. 3 hits.
    3.30.50.10. 1 hit.
    InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PRINTSi PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 2 hits.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a novel orphan receptor (GCNF) expressed during germ cell development."
      Chen F., Cooney A.J., Wang Y., Law S.W., O'Malley B.W.
      Mol. Endocrinol. 8:1434-1444(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DNA-BINDING, HOMODIMERIZATION, TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Testis.
    2. "RTR: a new member of the nuclear receptor superfamily that is highly expressed in murine testis."
      Hirose T., O'Brien D.A., Jetten A.M.
      Gene 152:247-251(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: CD-1.
      Tissue: Testis.
    3. "Genomic structure of the gene for mouse germ cell nuclear factor (GCNF)."
      Suesens U., Borgmeyer U.
      Genome Biol. 1:RESEARCH0006.1-RESEARCH0006.3(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
      Tissue: Liver.
    4. "Analysis of germ cell nuclear factor transcripts and protein expression during spermatogenesis."
      Yang G., Zhang Y.-L., Buchold G.M., Jetten A.M., O'Brien D.A.
      Biol. Reprod. 68:1620-1630(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Ola.
      Tissue: Testis.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    6. "Isolation of proteins that interact specifically with the retinoid X receptor: two novel orphan receptors."
      Seol W., Choi H.S., Moore D.D.
      Mol. Endocrinol. 9:72-85(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UIMC1.
    7. "xGCNF, a nuclear orphan receptor is expressed during neurulation in Xenopus laevis."
      Joos T.O., David R., Dreyer C.
      Mech. Dev. 60:45-57(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING, HOMODIMERIZATION.
    8. "RAP80: a novel nuclear protein that interacts with the retinoid-related testis-associated receptor."
      Yan Z., Kim Y.-S., Jetten A.M.
      J. Biol. Chem. 277:32379-32388(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UIMC1, MUTAGENESIS OF 246-SER-TYR-247; 254-LEU-PRO-255 AND 265-SER-THY-266.

    Entry informationi

    Entry nameiNR6A1_MOUSE
    AccessioniPrimary (citable) accession number: Q64249
    Secondary accession number(s): A2AQL8, A2AQM1, Q687Z3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3