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Q64249

- NR6A1_MOUSE

UniProt

Q64249 - NR6A1_MOUSE

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Protein
Nuclear receptor subfamily 6 group A member 1
Gene
Nr6a1, Gcnf
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi72 – 14776Nuclear receptor2 Publications
Add
BLAST
Zinc fingeri75 – 9521NR C4-type
Add
BLAST
Zinc fingeri111 – 13525NR C4-type
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. protein binding Source: HGNC
  3. protein homodimerization activity Source: UniProtKB
  4. sequence-specific DNA binding Source: UniProtKB
  5. sequence-specific DNA binding transcription factor activity Source: InterPro
  6. steroid hormone receptor activity Source: InterPro
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. gamete generation Source: UniProtKB
  3. multicellular organismal development Source: UniProtKB-KW
  4. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  5. spermatogenesis Source: UniProtKB-KW
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Receptor

Keywords - Biological processi

Differentiation, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_220962. POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 6 group A member 1
Alternative name(s):
Germ cell nuclear factor
Short name:
GCNF
Short name:
mGCNF
Retinoid receptor-related testis-specific receptor
Short name:
RTR
Gene namesi
Name:Nr6a1
Synonyms:Gcnf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1352459. Nr6a1.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi246 – 2472SY → GG: Abolishes interaction with RXRIP110. 1 Publication
Mutagenesisi254 – 2552LP → AA: Does not abolish interaction with RXRIP110. 1 Publication
Mutagenesisi265 – 2662SY → AA: Does not abolish interaction with RXRIP110. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495Nuclear receptor subfamily 6 group A member 1
PRO_0000053742Add
BLAST

Proteomic databases

PRIDEiQ64249.

PTM databases

PhosphoSiteiQ64249.

Expressioni

Tissue specificityi

Expressed in the germ cells of both the adult testis and ovary, being most abundant in spermatids.2 Publications

Gene expression databases

BgeeiQ64249.
CleanExiMM_NR6A1.
GenevestigatoriQ64249.

Interactioni

Subunit structurei

Homodimer. Interacts with UIMC1.4 Publications

Protein-protein interaction databases

BioGridi199868. 5 interactions.
STRINGi10090.ENSMUSP00000108498.

Structurei

3D structure databases

ProteinModelPortaliQ64249.
SMRiQ64249. Positions 72-484.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni187 – 26882Sufficient for interaction with UIMC1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG253308.
GeneTreeiENSGT00750000117458.
HOGENOMiHOG000113847.
HOVERGENiHBG052664.
InParanoidiQ64249.
KOiK08561.
OMAiHQLVSAE.
OrthoDBiEOG78WKRG.
PhylomeDBiQ64249.
TreeFamiTF350737.

Family and domain databases

Gene3Di1.10.565.10. 3 hits.
3.30.50.10. 1 hit.
InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q64249-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MERDERPPSG GGGGGGSAGF LEPPAALPPP PRNGFCQDEL AELDPGTNGE    50
TDSLTLGQGH IPVSVPDDRA EQRTCLICGD RATGLHYGII SCEGCKGFFK 100
RSICNKRVYR CSRDKNCVMS RKQRNRCQYC RLLKCLQMGM NRKAIREDGM 150
PGGRNKSIGP VQISEEEIER IMSGQEFEEE ANHWSNHGDS DHSSPGNRAS 200
ESNQPSPGST LSSSRSVELN GFMAFRDQYM GMSVPPHYQY IPHLFSYSGH 250
SPLLPPQARS LDPQSYSLIH QLMSAEDLEP LGTPMLIEDG YAVTQAELFA 300
LLCRLADELL FRQIAWIKKL PFFCELSIKD YTCLLSSTWQ ELILLSSLTV 350
YSKQIFGELA DVTAKYSPSD EELHRFSDEG MEVIERLIYL YHKFHQLKVS 400
NEEYACMKAI NFLNQDIRGL TSASQLEQLN KRYWYICQDF TEYKYTHQPN 450
RFPDLMMCLP EIRYIAGKMV NVPLEQLPLL FKVVLHSCKT STVKE 495
Length:495
Mass (Da):55,976
Last modified:November 1, 1996 - v1
Checksum:iECB0AB3E99E42661
GO
Isoform 2 (identifier: Q64249-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     48-62: Missing.
     63-63: V → I

Note: No experimental confirmation available.

Show »
Length:480
Mass (Da):54,469
Checksum:i17DA7AC5DB297A23
GO
Isoform 3 (identifier: Q64249-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: Missing.
     58-62: QGHIP → METWE

Note: No experimental confirmation available.

Show »
Length:438
Mass (Da):50,445
Checksum:i78B62010C39F7310
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5757Missing in isoform 3.
VSP_025928Add
BLAST
Alternative sequencei48 – 6215Missing in isoform 2.
VSP_025929Add
BLAST
Alternative sequencei58 – 625QGHIP → METWE in isoform 3.
VSP_025930
Alternative sequencei63 – 631V → I in isoform 2.
VSP_025931

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14666 mRNA. Translation: AAA92682.1.
U09563 mRNA. Translation: AAA64746.1.
AF254821
, AF254575, AF254815, AF254816, AF254817, AF254818, AF254819, AF254820 Genomic DNA. Translation: AAG24485.1.
AF390896 mRNA. Translation: AAM55193.1.
AF390900
, AF390897, AF390898, AF390899 Genomic DNA. Translation: AAM95878.1.
AL844842, AL935155 Genomic DNA. Translation: CAM21928.1.
AL844842, AL935155 Genomic DNA. Translation: CAM21929.1.
AL844842 Genomic DNA. Translation: CAM21933.1.
AL935155, AL844842 Genomic DNA. Translation: CAM21313.1.
AL935155, AL844842 Genomic DNA. Translation: CAM21314.1.
CCDSiCCDS16012.1. [Q64249-1]
CCDS50581.1. [Q64249-3]
PIRiA57053.
RefSeqiNP_001153020.1. NM_001159548.1. [Q64249-3]
NP_034394.1. NM_010264.4. [Q64249-1]
XP_006497771.1. XM_006497708.1. [Q64249-2]
UniGeneiMm.439703.

Genome annotation databases

EnsembliENSMUST00000076275; ENSMUSP00000075624; ENSMUSG00000063972. [Q64249-1]
ENSMUST00000112877; ENSMUSP00000108498; ENSMUSG00000063972. [Q64249-1]
ENSMUST00000168098; ENSMUSP00000126009; ENSMUSG00000063972. [Q64249-3]
GeneIDi14536.
KEGGimmu:14536.
UCSCiuc012bus.1. mouse. [Q64249-3]
uc012buu.1. mouse. [Q64249-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14666 mRNA. Translation: AAA92682.1 .
U09563 mRNA. Translation: AAA64746.1 .
AF254821
, AF254575 , AF254815 , AF254816 , AF254817 , AF254818 , AF254819 , AF254820 Genomic DNA. Translation: AAG24485.1 .
AF390896 mRNA. Translation: AAM55193.1 .
AF390900
, AF390897 , AF390898 , AF390899 Genomic DNA. Translation: AAM95878.1 .
AL844842 , AL935155 Genomic DNA. Translation: CAM21928.1 .
AL844842 , AL935155 Genomic DNA. Translation: CAM21929.1 .
AL844842 Genomic DNA. Translation: CAM21933.1 .
AL935155 , AL844842 Genomic DNA. Translation: CAM21313.1 .
AL935155 , AL844842 Genomic DNA. Translation: CAM21314.1 .
CCDSi CCDS16012.1. [Q64249-1 ]
CCDS50581.1. [Q64249-3 ]
PIRi A57053.
RefSeqi NP_001153020.1. NM_001159548.1. [Q64249-3 ]
NP_034394.1. NM_010264.4. [Q64249-1 ]
XP_006497771.1. XM_006497708.1. [Q64249-2 ]
UniGenei Mm.439703.

3D structure databases

ProteinModelPortali Q64249.
SMRi Q64249. Positions 72-484.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199868. 5 interactions.
STRINGi 10090.ENSMUSP00000108498.

PTM databases

PhosphoSitei Q64249.

Proteomic databases

PRIDEi Q64249.

Protocols and materials databases

DNASUi 14536.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000076275 ; ENSMUSP00000075624 ; ENSMUSG00000063972 . [Q64249-1 ]
ENSMUST00000112877 ; ENSMUSP00000108498 ; ENSMUSG00000063972 . [Q64249-1 ]
ENSMUST00000168098 ; ENSMUSP00000126009 ; ENSMUSG00000063972 . [Q64249-3 ]
GeneIDi 14536.
KEGGi mmu:14536.
UCSCi uc012bus.1. mouse. [Q64249-3 ]
uc012buu.1. mouse. [Q64249-1 ]

Organism-specific databases

CTDi 2649.
MGIi MGI:1352459. Nr6a1.

Phylogenomic databases

eggNOGi NOG253308.
GeneTreei ENSGT00750000117458.
HOGENOMi HOG000113847.
HOVERGENi HBG052664.
InParanoidi Q64249.
KOi K08561.
OMAi HQLVSAE.
OrthoDBi EOG78WKRG.
PhylomeDBi Q64249.
TreeFami TF350737.

Enzyme and pathway databases

Reactomei REACT_220962. POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.

Miscellaneous databases

ChiTaRSi NR6A1. mouse.
NextBioi 286194.
PROi Q64249.
SOURCEi Search...

Gene expression databases

Bgeei Q64249.
CleanExi MM_NR6A1.
Genevestigatori Q64249.

Family and domain databases

Gene3Di 1.10.565.10. 3 hits.
3.30.50.10. 1 hit.
InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view ]
Pfami PF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view ]
PRINTSi PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTi SM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view ]
SUPFAMi SSF48508. SSF48508. 2 hits.
PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a novel orphan receptor (GCNF) expressed during germ cell development."
    Chen F., Cooney A.J., Wang Y., Law S.W., O'Malley B.W.
    Mol. Endocrinol. 8:1434-1444(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DNA-BINDING, HOMODIMERIZATION, TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Testis.
  2. "RTR: a new member of the nuclear receptor superfamily that is highly expressed in murine testis."
    Hirose T., O'Brien D.A., Jetten A.M.
    Gene 152:247-251(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: CD-1.
    Tissue: Testis.
  3. "Genomic structure of the gene for mouse germ cell nuclear factor (GCNF)."
    Suesens U., Borgmeyer U.
    Genome Biol. 1:RESEARCH0006.1-RESEARCH0006.3(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
    Tissue: Liver.
  4. "Analysis of germ cell nuclear factor transcripts and protein expression during spermatogenesis."
    Yang G., Zhang Y.-L., Buchold G.M., Jetten A.M., O'Brien D.A.
    Biol. Reprod. 68:1620-1630(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Ola.
    Tissue: Testis.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. "Isolation of proteins that interact specifically with the retinoid X receptor: two novel orphan receptors."
    Seol W., Choi H.S., Moore D.D.
    Mol. Endocrinol. 9:72-85(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UIMC1.
  7. "xGCNF, a nuclear orphan receptor is expressed during neurulation in Xenopus laevis."
    Joos T.O., David R., Dreyer C.
    Mech. Dev. 60:45-57(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING, HOMODIMERIZATION.
  8. "RAP80: a novel nuclear protein that interacts with the retinoid-related testis-associated receptor."
    Yan Z., Kim Y.-S., Jetten A.M.
    J. Biol. Chem. 277:32379-32388(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UIMC1, MUTAGENESIS OF 246-SER-TYR-247; 254-LEU-PRO-255 AND 265-SER-THY-266.

Entry informationi

Entry nameiNR6A1_MOUSE
AccessioniPrimary (citable) accession number: Q64249
Secondary accession number(s): A2AQL8, A2AQM1, Q687Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi