Q64244 (CD38_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-ribosyl cyclase 1
EC=3.2.2.5

Alternative name(s):
CD38H
Cyclic ADP-ribose hydrolase 1
Short name=cADPr hydrolase 1
CD_antigen=CD38

Gene names

Name:

Cd38

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Ref.4 Regulates osteoclastic bone resorption, probably via production of cyclic ADP-ribose and triggering of a cytosolic calcium ion signal through ryanodine receptor activation. Ref.4
Catalytic activity	NAD+ + H2O = ADP-ribose + nicotinamide.
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Spleen, liver, heart, thymus, thyroid gland, ileum, colon, cerebellum, salivary gland, adrenal gland, jejunum, islets of Langerhans and osteoclasts. Ref.4
Sequence similarities	Belongs to the ADP-ribosyl cyclase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	B cell receptor signaling pathway Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from mutant phenotype PubMed 18719074. Source: RGD female pregnancy Inferred from expression pattern PubMed 14998907. Source: RGD long term synaptic depression Inferred from mutant phenotype PubMed 10097163. Source: RGD negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of bone resorption Inferred from direct assay Ref.4. Source: RGD negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara positive regulation of B cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of cell growth Inferred from mutant phenotype PubMed 18719074. Source: RGD positive regulation of insulin secretion Inferred from mutant phenotype PubMed 9664081. Source: RGD positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara positive regulation of vasoconstriction Inferred from mutant phenotype PubMed 18524860. Source: RGD response to drug Inferred from electronic annotation. Source: Compara response to estradiol stimulus Inferred from expression pattern PubMed 14998907. Source: RGD response to hydroperoxide Inferred from direct assay PubMed 12893282. Source: RGD response to hypoxia Inferred from direct assay PubMed 12111041. Source: RGD response to interleukin-1 Inferred from expression pattern PubMed 11399650. Source: RGD response to progesterone stimulus Inferred from expression pattern PubMed 14998907. Source: RGD response to retinoic acid Inferred from expression pattern PubMed 11399650. Source: RGD
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay PubMed 11733184. Source: RGD plasma membrane Inferred from direct assay PubMed 17200192. Source: RGD
Molecular_function	NAD+ nucleosidase activity Inferred from direct assay PubMed 11733184. Source: RGD nucleotide binding Inferred from electronic annotation. Source: InterPro phosphorus-oxygen lyase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	ADP-ribosyl cyclase 1		PRO_0000144070
Regions
	Topological domain	1 – 21	21	Cytoplasmic Potential
	Transmembrane	22 – 44	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	45 – 303	259	Extracellular Potential
Sites
	Active site	122	1	By similarity
	Active site	204	1	By similarity
Amino acid modifications
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	123	1	N-linked (GlcNAc...) Potential
	Glycosylation	212	1	N-linked (GlcNAc...) Potential
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Disulfide bond	69 ↔ 85		By similarity
	Disulfide bond	102 ↔ 183		By similarity
	Disulfide bond	163 ↔ 176		By similarity
	Disulfide bond	257 ↔ 278		By similarity
	Disulfide bond	290 ↔ 299		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64244 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 84F23AD3094871C5	FASTA	303	34,436
	10 20 30 40 50 60 MANYEFSQVS EDRPGCRLTR KAQIGLGVGL LLLVALVVVV VIVLWPRSPL VWKGKPTTKH 70 80 90 100 110 120 FADIILGRCL IYTQILRPEM RDQDCKKILS TFKRGFISKN PCNITNEDYA PLVKLVTQTI 130 140 150 160 170 180 PCNKTLFWSK SKHLAHQYTW IQGKMFTLED TLLGYIADDL RWCGDPSTSD MNYDSCPHWS 190 200 210 220 230 240 ENCPNNPVAV FWNVISQKFA EDACGVVQVM LNGSLSEPFY RNSTFGSVEV FNLDPNKVHK 250 260 270 280 290 300 LQAWVMHDIK GTSSNACSSP SINELKSIVN KRNMIFACQD NYRPVRFLQC VKNPEHPSCR LNV

References

[1]	"A cloned rat CD38-homologous protein and its expression in pancreatic islets." Li Q., Yamada Y., Yasuda K., Ihara Y., Okamoto Y., Kaisaki P.J., Watanabe R., Ikeda H., Tsuda K., Seino Y. Biochem. Biophys. Res. Commun. 202:629-636(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.
[2]	Erratum Li Q., Yamada Y., Yasuda K., Ihara Y., Okamoto Y., Kaisaki P.J., Watanabe R., Ikeda H., Tsuda K., Seino Y. Biochem. Biophys. Res. Commun. 204:1001-1001(1994)
[3]	"Cloning and characterization of cDNA encoding rat ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase (homologue to human CD38) from islets of Langerhans." Koguma T., Takasawa S., Tohgo A., Karasawa T., Furuya Y., Yonekura H., Okamoto H. Biochim. Biophys. Acta 1223:160-162(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Pancreatic islet.
[4]	"CD38/ADP-ribosyl cyclase: a new role in the regulation of osteoclastic bone resorption." Sun L., Adebanjo O.A., Moonga B.S., Corisdeo S., Anandatheerthavarada H.K., Biswas G., Arakawa T., Hakeda Y., Koval A., Sodam B., Bevis P.J.R., Moser A.J., Lai F.A., Epstein S., Troen B.R., Kumegawa M., Zaidi M. J. Cell Biol. 146:1161-1172(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]	Erratum Sun L., Adebanjo O.A., Moonga B.S., Corisdeo S., Anandatheerthavarada H.K., Biswas G., Arakawa T., Hakeda Y., Koval A., Sodam B., Bevis P.J.R., Moser A.J., Lai F.A., Epstein S., Troen B.R., Kumegawa M., Zaidi M. J. Cell Biol. 146:1391-1392(1999)
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D30795 mRNA. Translation: BAA06457.1. D29646 mRNA. Translation: BAA06129.1.
IPI	IPI00210901.
PIR	JC2410.
RefSeq	NP_037259.1. NM_013127.1.
UniGene	Rn.11414.
3D structure databases
ProteinModelPortal	Q64244.
SMR	Q64244. Positions 50-282.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000004121.
Proteomic databases
PRIDE	Q64244.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000004121; ENSRNOP00000004121; ENSRNOG00000003069.
GeneID	25668.
KEGG	rno:25668.
Organism-specific databases
CTD	952.
RGD	2303. Cd38.
Phylogenomic databases
eggNOG	NOG42596.
GeneTree	ENSGT00390000017291.
HOGENOM	HOG000293141.
HOVERGEN	HBG005277.
InParanoid	Q64244.
KO	K01242.
OMA	FLQCVKN.
OrthoDB	EOG4H9XMX.
Enzyme and pathway databases
SABIO-RK	Q64244.
Gene expression databases
Genevestigator	Q64244.
GermOnline	ENSRNOG00000003069. Rattus norvegicus.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR003193. ADP-ribosyl_cyclase. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR10912. PTHR10912. 1 hit.
Pfam	PF02267. Rib_hydrolayse. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607593.

Entry information

Entry name

CD38_RAT

Accession

Primary (citable) accession number: Q64244

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program