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Q64213

- SF01_MOUSE

UniProt

Q64213 - SF01_MOUSE

Protein

Splicing factor 1

Gene

Sf1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 6 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri277 – 29620CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. Leydig cell differentiation Source: MGI
    2. male sex determination Source: MGI
    3. mRNA processing Source: UniProtKB-KW
    4. negative regulation of smooth muscle cell proliferation Source: Ensembl
    5. regulation of steroid biosynthetic process Source: MGI
    6. regulation of transcription, DNA-templated Source: UniProtKB-KW
    7. RNA splicing Source: UniProtKB-KW
    8. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    mRNA processing, mRNA splicing, Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Splicing factor 1
    Alternative name(s):
    CW17
    Mammalian branch point-binding protein
    Short name:
    BBP
    Short name:
    mBBP
    Transcription factor ZFM1
    Short name:
    mZFM
    Zinc finger gene in MEN1 locus
    Zinc finger protein 162
    Gene namesi
    Name:Sf1
    Synonyms:Zfm1, Zfp162
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1095403. Sf1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: MGI
    3. spliceosomal complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 653652Splicing factor 1PRO_0000050130Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei20 – 201Phosphoserine; by PKGBy similarity
    Modified residuei80 – 801Phosphoserine3 Publications
    Modified residuei82 – 821Phosphoserine3 Publications
    Modified residuei87 – 871Phosphotyrosine1 Publication

    Post-translational modificationi

    Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ64213.
    PaxDbiQ64213.
    PRIDEiQ64213.

    PTM databases

    PhosphoSiteiQ64213.

    Expressioni

    Tissue specificityi

    Detected at intermediate levels in spleen. Lower levels in heart, kidney, brain, liver, testis, bone marrow, adrenal gland, lymph nodes, pancreas and thymus.

    Gene expression databases

    ArrayExpressiQ64213.
    BgeeiQ64213.
    CleanExiMM_SF1.
    GenevestigatoriQ64213.

    Interactioni

    Subunit structurei

    Binds U2AF2. Interacts with U1 snRNA. Interacts with RBM17. Binds EWSR1, FUS and TAF15 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-60453N.
    IntActiQ64213. 3 interactions.
    MINTiMINT-4134251.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64213.
    SMRiQ64213. Positions 1-255.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini141 – 22282KHPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi15 – 195Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi324 – 585262Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the BBP/SF1 family.Curated
    Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation
    Contains 1 KH domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri277 – 29620CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5176.
    GeneTreeiENSGT00550000074434.
    HOGENOMiHOG000237321.
    HOVERGENiHBG063318.
    InParanoidiQ64213.
    OMAiCLSPNRA.
    OrthoDBiEOG7K0ZC4.
    TreeFamiTF319159.

    Family and domain databases

    Gene3Di3.30.1370.10. 1 hit.
    4.10.60.10. 1 hit.
    InterProiIPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF00013. KH_1. 1 hit.
    PF00098. zf-CCHC. 1 hit.
    [Graphical view]
    SMARTiSM00322. KH. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF54791. SSF54791. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50158. ZF_CCHC. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform CW17 (identifier: Q64213-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER    50
    AYIVQLQIED LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR 100
    TRKKLEEERH TLITEMVALN PDFKPPADYK PPATRVSDKV MIPQDEYPEI 150
    NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK GSVKEGKVGR KDGQMLPGED 200
    EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR KMQLRELARL 250
    NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP 300
    QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA 350
    SNPPPPSLMS TTQSRPPWMN SGPSENRPYH GMHGGGPGGP GGGPHSFPHP 400
    LPSLTGGHGG HPMQHNPNGP PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY 450
    LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP PPPPPSGQPP PPPSGPLPPW 500
    QQQQQQPPPP PPPSSSMASS TPLPWQQNTT TTTTSAGTGS IPPWQQQQAA 550
    AAASPGTPQM QGNPTMVPLP PGVQPPLPPG APPPPPCSIE CLLCLLSSPN 600
    SLCLSPNRAA RIPPRGSDGP SHESEDFPRP LVTLPGRQPQ QRPWWTGWFG 650
    KAA 653
    Length:653
    Mass (Da):70,403
    Last modified:July 27, 2011 - v6
    Checksum:i5C159302822509D1
    GO
    Isoform CW17E (identifier: Q64213-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         529-653: TTTTTTSAGT...WWTGWFGKAA → SLPAAAMARAMRVRTFRAHW

    Show »
    Length:548
    Mass (Da):59,657
    Checksum:i8965858D8C10D170
    GO
    Isoform 3 (identifier: Q64213-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         586-653: PCSIECLLCL...WWTGWFGKAA → PPPPPGSAGM...PAPPPPPPQN

    Note: No experimental confirmation available.

    Show »
    Length:639
    Mass (Da):68,347
    Checksum:i20885630AB6292B1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti184 – 1841K → E in CAA59797. (PubMed:9212169)Curated
    Sequence conflicti184 – 1841K → E in CAA73359. (PubMed:10360842)Curated
    Sequence conflicti509 – 5091P → S in AAH55370. (PubMed:15489334)Curated
    Sequence conflicti524 – 5241P → L in AAH09091. (PubMed:15489334)Curated
    Sequence conflicti586 – 5861P → T in CAA73359. (PubMed:10360842)Curated
    Sequence conflicti586 – 5861P → T in CAA70113. (PubMed:10360842)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei529 – 653125TTTTT…FGKAA → SLPAAAMARAMRVRTFRAHW in isoform CW17E. 2 PublicationsVSP_050424Add
    BLAST
    Alternative sequencei586 – 65368PCSIE…FGKAA → PPPPPGSAGMMYAPPPPPPP PMDPSNFVTMMGMGVAGMPP FGMPPAPPPPPPQN in isoform 3. 1 PublicationVSP_008840Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80159 mRNA. Translation: CAA56440.1.
    X85802 mRNA. Translation: CAA59797.1.
    Y08907 Genomic DNA. Translation: CAA70113.1.
    Y12838 Genomic DNA. Translation: CAA73359.1.
    AC127556 Genomic DNA. No translation available.
    AC167245 Genomic DNA. No translation available.
    BC009091 mRNA. Translation: AAH09091.1.
    BC055370 mRNA. Translation: AAH55370.1.
    CCDSiCCDS50368.1. [Q64213-3]
    CCDS50369.1. [Q64213-2]
    UniGeneiMm.256422.

    Genome annotation databases

    EnsembliENSMUST00000131252; ENSMUSP00000121309; ENSMUSG00000024949. [Q64213-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80159 mRNA. Translation: CAA56440.1 .
    X85802 mRNA. Translation: CAA59797.1 .
    Y08907 Genomic DNA. Translation: CAA70113.1 .
    Y12838 Genomic DNA. Translation: CAA73359.1 .
    AC127556 Genomic DNA. No translation available.
    AC167245 Genomic DNA. No translation available.
    BC009091 mRNA. Translation: AAH09091.1 .
    BC055370 mRNA. Translation: AAH55370.1 .
    CCDSi CCDS50368.1. [Q64213-3 ]
    CCDS50369.1. [Q64213-2 ]
    UniGenei Mm.256422.

    3D structure databases

    ProteinModelPortali Q64213.
    SMRi Q64213. Positions 1-255.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60453N.
    IntActi Q64213. 3 interactions.
    MINTi MINT-4134251.

    PTM databases

    PhosphoSitei Q64213.

    Proteomic databases

    MaxQBi Q64213.
    PaxDbi Q64213.
    PRIDEi Q64213.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000131252 ; ENSMUSP00000121309 ; ENSMUSG00000024949 . [Q64213-3 ]

    Organism-specific databases

    MGIi MGI:1095403. Sf1.

    Phylogenomic databases

    eggNOGi COG5176.
    GeneTreei ENSGT00550000074434.
    HOGENOMi HOG000237321.
    HOVERGENi HBG063318.
    InParanoidi Q64213.
    OMAi CLSPNRA.
    OrthoDBi EOG7K0ZC4.
    TreeFami TF319159.

    Miscellaneous databases

    ChiTaRSi SF1. mouse.
    PROi Q64213.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64213.
    Bgeei Q64213.
    CleanExi MM_SF1.
    Genevestigatori Q64213.

    Family and domain databases

    Gene3Di 3.30.1370.10. 1 hit.
    4.10.60.10. 1 hit.
    InterProi IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF00013. KH_1. 1 hit.
    PF00098. zf-CCHC. 1 hit.
    [Graphical view ]
    SMARTi SM00322. KH. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54791. SSF54791. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50158. ZF_CCHC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Enhanced expression in spleen macrophages of the mouse homolog to the human putative tumor suppressor gene ZFM1."
      Wrehlke C., Schmitt-Wrede H.-P., Qiao Z.D., Wunderlich F.
      DNA Cell Biol. 16:761-767(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS CW17 AND CW17E).
      Strain: C57BL/10.
      Tissue: Spleen.
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C57B1/10.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS CW17E AND 3).
      Strain: C3H/He.
      Tissue: Mammary tumor and Osteoblast.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80; SER-82 AND TYR-87, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiSF01_MOUSE
    AccessioniPrimary (citable) accession number: Q64213
    Secondary accession number(s): E9QK02
    , O08817, P70167, Q61454, Q921Z4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 7, 2003
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 123 of the entry and version 6 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3