Splicing factor 1 - Mus musculus (Mouse)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Splicing factor 1

Alternative name(s):
CW17
Mammalian branch point-binding protein
Short name=BBP
Short name=mBBP
Transcription factor ZFM1
Short name=mZFM
Zinc finger gene in MEN1 locus
Zinc finger protein 162

Gene names

Name:	Sf1
Synonyms:	Zfm1, Zfp162

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	653 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor By similarity.
Subunit structure	Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15 By similarity.
Subcellular location	Nucleus.
Tissue specificity	Detected at intermediate levels in spleen. Lower levels in heart, kidney, brain, liver, testis, bone marrow, adrenal gland, lymph nodes, pancreas and thymus.
Post-translational modification	Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly By similarity.
Sequence similarities	Belongs to the BBP/SF1 family. Contains 1 CCHC-type zinc finger. Contains 1 KH domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Molecular function	Repressor
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Leydig cell differentiation Inferred from genetic interaction PubMed 17495005. Source: MGI RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW cell cycle process Inferred from genetic interaction PubMed 17495005. Source: MGI mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW male sex determination Inferred from genetic interaction PubMed 17495005. Source: MGI regulation of steroid biosynthetic process Inferred from genetic interaction PubMed 17495005. Source: MGI regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from sequence or structural similarity PubMed 18559850. Source: MGI nucleus Inferred from sequence or structural similarity PubMed 18559850. Source: MGI spliceosomal complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform CW17 (identifier: Q64213-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform CW17E (identifier: Q64213-2) The sequence of this isoform differs from the canonical sequence as follows: 529-653: TTTTTTSAGT...WWTGWFGKAA → SLPAAAMARAMRVRTFRAHW

Isoform 3 (identifier: Q64213-3) The sequence of this isoform differs from the canonical sequence as follows: 586-653: PCSIECLLCL...WWTGWFGKAA → PPPPPGSAGM...PAPPPPPPQN
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

Removed By similarity

Chain

2 – 653

652

Splicing factor 1

PRO_0000050130

Regions

Domain

141 – 222

82

KH

Zinc finger

277 – 296

20

CCHC-type

Motif

15 – 19

5

Nuclear localization signal Potential

Compositional bias

324 – 585

262

Pro-rich

Amino acid modifications

Modified residue

2

1

N-acetylalanine By similarity

Modified residue

20

1

Phosphoserine; by PKG By similarity

Modified residue

80

1

Phosphoserine Ref.5 Ref.6 Ref.7 Ref.8 Ref.10

Modified residue

82

1

Phosphoserine Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Natural variations

Alternative sequence

529 – 653

125

TTTTT…FGKAA → SLPAAAMARAMRVRTFRAHW in isoform CW17E.

VSP_050424

Alternative sequence

586 – 653

68

PCSIE…FGKAA → PPPPPGSAGMMYAPPPPPPP PMDPSNFVTMMGMGVAGMPP FGMPPAPPPPPPQN in isoform 3.

VSP_008840

Experimental info

Sequence conflict

184

1

K → E in CAA59797. Ref.1

Sequence conflict

184

1

K → E in CAA73359. Ref.2

Sequence conflict

509

1

P → S in AAH55370. Ref.4

Sequence conflict

524

1

P → L in AAH09091. Ref.4

Sequence conflict

586

1

P → T in CAA73359. Ref.2

Sequence conflict

586

1

P → T in CAA70113. Ref.2

Sequences

Sequence		Length	Mass (Da)
Isoform CW17 [UniParc]. Last modified July 27, 2011. Version 6. Checksum: 5C159302822509D1 Show »	FASTA	653	70,403
10 20 30 40 50 60 MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER AYIVQLQIED 70 80 90 100 110 120 LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR TRKKLEEERH TLITEMVALN 130 140 150 160 170 180 PDFKPPADYK PPATRVSDKV MIPQDEYPEI NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK 190 200 210 220 230 240 GSVKEGKVGR KDGQMLPGED EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR 250 260 270 280 290 300 KMQLRELARL NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP 310 320 330 340 350 360 QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA SNPPPPSLMS 370 380 390 400 410 420 TTQSRPPWMN SGPSENRPYH GMHGGGPGGP GGGPHSFPHP LPSLTGGHGG HPMQHNPNGP 430 440 450 460 470 480 PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP 490 500 510 520 530 540 PPPPPSGQPP PPPSGPLPPW QQQQQQPPPP PPPSSSMASS TPLPWQQNTT TTTTSAGTGS 550 560 570 580 590 600 IPPWQQQQAA AAASPGTPQM QGNPTMVPLP PGVQPPLPPG APPPPPCSIE CLLCLLSSPN 610 620 630 640 650 SLCLSPNRAA RIPPRGSDGP SHESEDFPRP LVTLPGRQPQ QRPWWTGWFG KAA « Hide
Isoform CW17E [UniParc]. Checksum: 8965858D8C10D170 Show »	FASTA	548	59,657
10 20 30 40 50 60 MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER AYIVQLQIED 70 80 90 100 110 120 LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR TRKKLEEERH TLITEMVALN 130 140 150 160 170 180 PDFKPPADYK PPATRVSDKV MIPQDEYPEI NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK 190 200 210 220 230 240 GSVKEGKVGR KDGQMLPGED EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR 250 260 270 280 290 300 KMQLRELARL NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP 310 320 330 340 350 360 QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA SNPPPPSLMS 370 380 390 400 410 420 TTQSRPPWMN SGPSENRPYH GMHGGGPGGP GGGPHSFPHP LPSLTGGHGG HPMQHNPNGP 430 440 450 460 470 480 PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP 490 500 510 520 530 540 PPPPPSGQPP PPPSGPLPPW QQQQQQPPPP PPPSSSMASS TPLPWQQNSL PAAAMARAMR VRTFRAHW « Hide
Isoform 3 [UniParc]. Checksum: 20885630AB6292B1 Show »	FASTA	639	68,347
10 20 30 40 50 60 MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER AYIVQLQIED 70 80 90 100 110 120 LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR TRKKLEEERH TLITEMVALN 130 140 150 160 170 180 PDFKPPADYK PPATRVSDKV MIPQDEYPEI NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK 190 200 210 220 230 240 GSVKEGKVGR KDGQMLPGED EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR 250 260 270 280 290 300 KMQLRELARL NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP 310 320 330 340 350 360 QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA SNPPPPSLMS 370 380 390 400 410 420 TTQSRPPWMN SGPSENRPYH GMHGGGPGGP GGGPHSFPHP LPSLTGGHGG HPMQHNPNGP 430 440 450 460 470 480 PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP 490 500 510 520 530 540 PPPPPSGQPP PPPSGPLPPW QQQQQQPPPP PPPSSSMASS TPLPWQQNTT TTTTSAGTGS 550 560 570 580 590 600 IPPWQQQQAA AAASPGTPQM QGNPTMVPLP PGVQPPLPPG APPPPPPPPP GSAGMMYAPP 610 620 630 PPPPPPMDPS NFVTMMGMGV AGMPPFGMPP APPPPPPQN « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Enhanced expression in spleen macrophages of the mouse homolog to the human putative tumor suppressor gene ZFM1." Wrehlke C., Schmitt-Wrede H.-P., Qiao Z.D., Wunderlich F. DNA Cell Biol. 16:761-767(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS CW17 AND CW17E). Strain: C57BL/10. Tissue: Spleen.
[2]	"Genomic organization of mouse gene zfp162 (mzfm)." Wrehlke C., Wiedemeyer W.-R., Schmitt-Wrede H.-P., Mincheva A., Lichter P., Wunderlich F. DNA Cell Biol. 18:419-428(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57B1/10.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS CW17E AND 3). Strain: C3H/He. Tissue: Mammary tumor and Osteoblast.
[5]	"Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line." Shu H., Chen S., Bi Q., Mumby M., Brekken D.L. Mol. Cell. Proteomics 3:279-286(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[6]	"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells." Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D. J. Proteome Res. 6:3174-3186(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, MASS SPECTROMETRY. Tissue: Teratocarcinoma.
[7]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, MASS SPECTROMETRY. Tissue: Melanoma.
[9]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, MASS SPECTROMETRY. Tissue: Macrophage.
[10]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80159 mRNA. Translation: CAA56440.1. X85802 mRNA. Translation: CAA59797.1. Y08907 Genomic DNA. Translation: CAA70113.1. Y12838 Genomic DNA. Translation: CAA73359.1. AC127556 Genomic DNA. No translation available. AC167245 Genomic DNA. No translation available. BC009091 mRNA. Translation: AAH09091.1. BC055370 mRNA. Translation: AAH55370.1.
IPI	IPI00116284. IPI00387376. IPI01008154.
UniGene	Mm.256422.
3D structure databases
ProteinModelPortal	Q64213.
SMR	Q64213. Positions 134-255.
ModBase	Search...
PTM databases
PhosphoSite	Q64213.
Proteomic databases
PaxDb	Q64213.
PRIDE	Q64213.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000131252; ENSMUSP00000121309; ENSMUSG00000024949.
Organism-specific databases
MGI	MGI:1095403. Sf1.
Phylogenomic databases
eggNOG	COG5176.
GeneTree	ENSGT00550000074434.
HOGENOM	HOG000237321.
HOVERGEN	HBG063318.
InParanoid	Q64213.
OMA	GDGPSHE.
Gene expression databases
ArrayExpress	Q64213.
Bgee	Q64213.
CleanEx	MM_SF1.
Genevestigator	Q64213.
GermOnline	ENSMUSG00000024949. Mus musculus.
Family and domain databases
Gene3D	4.10.60.10. 1 hit.
InterPro	IPR004087. KH_dom. IPR004088. KH_dom_type_1. IPR001878. Znf_CCHC. [Graphical view]
Pfam	PF00013. KH_1. 1 hit. PF00098. zf-CCHC. 1 hit. [Graphical view]
SMART	SM00322. KH. 1 hit. SM00343. ZnF_C2HC. 1 hit. [Graphical view]
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50158. ZF_CCHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SF1. mouse.
SOURCE	Search...

Entry information

Entry name

SF01_MOUSE

Accession

Primary (citable) accession number: Q64213
Secondary accession number(s): E9QK02

Q921Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 110 of the entry and version 6 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents