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Q64213 (SF01_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Splicing factor 1
Alternative name(s):
CW17
Mammalian branch point-binding protein
Short name=BBP
Short name=mBBP
Transcription factor ZFM1
Short name=mZFM
Zinc finger gene in MEN1 locus
Zinc finger protein 162
Gene names
Name:Sf1
Synonyms:Zfm1, Zfp162
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length653 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor By similarity.

Subunit structure

Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15 By similarity.

Subcellular location

Nucleus.

Tissue specificity

Detected at intermediate levels in spleen. Lower levels in heart, kidney, brain, liver, testis, bone marrow, adrenal gland, lymph nodes, pancreas and thymus.

Post-translational modification

Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly By similarity.

Sequence similarities

Belongs to the BBP/SF1 family.

Contains 1 CCHC-type zinc finger.

Contains 1 KH domain.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
Transcription
Transcription regulation
   Cellular componentNucleus
Spliceosome
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandMetal-binding
RNA-binding
Zinc
   Molecular functionRepressor
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processLeydig cell differentiation

Inferred from genetic interaction PubMed 17495005. Source: MGI

RNA splicing

Inferred from electronic annotation. Source: UniProtKB-KW

mRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

male sex determination

Inferred from genetic interaction PubMed 17495005. Source: MGI

negative regulation of smooth muscle cell proliferation

Inferred from electronic annotation. Source: Ensembl

regulation of steroid biosynthetic process

Inferred from genetic interaction PubMed 17495005. Source: MGI

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity PubMed 18559850. Source: MGI

nucleus

Inferred from sequence or structural similarity PubMed 18559850. Source: MGI

spliceosomal complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform CW17 (identifier: Q64213-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform CW17E (identifier: Q64213-2)

The sequence of this isoform differs from the canonical sequence as follows:
     529-653: TTTTTTSAGT...WWTGWFGKAA → SLPAAAMARAMRVRTFRAHW
Isoform 3 (identifier: Q64213-3)

The sequence of this isoform differs from the canonical sequence as follows:
     586-653: PCSIECLLCL...WWTGWFGKAA → PPPPPGSAGM...PAPPPPPPQN
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 653652Splicing factor 1
PRO_0000050130

Regions

Domain141 – 22282KH
Zinc finger277 – 29620CCHC-type
Motif15 – 195Nuclear localization signal Potential
Compositional bias324 – 585262Pro-rich

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue201Phosphoserine; by PKG By similarity
Modified residue801Phosphoserine Ref.5 Ref.6 Ref.7
Modified residue821Phosphoserine Ref.5 Ref.6 Ref.7
Modified residue871Phosphotyrosine Ref.6

Natural variations

Alternative sequence529 – 653125TTTTT…FGKAA → SLPAAAMARAMRVRTFRAHW in isoform CW17E.
VSP_050424
Alternative sequence586 – 65368PCSIE…FGKAA → PPPPPGSAGMMYAPPPPPPP PMDPSNFVTMMGMGVAGMPP FGMPPAPPPPPPQN in isoform 3.
VSP_008840

Experimental info

Sequence conflict1841K → E in CAA59797. Ref.1
Sequence conflict1841K → E in CAA73359. Ref.2
Sequence conflict5091P → S in AAH55370. Ref.4
Sequence conflict5241P → L in AAH09091. Ref.4
Sequence conflict5861P → T in CAA73359. Ref.2
Sequence conflict5861P → T in CAA70113. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform CW17 [UniParc].

Last modified July 27, 2011. Version 6.
Checksum: 5C159302822509D1

FASTA65370,403
        10         20         30         40         50         60 
MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER AYIVQLQIED 

        70         80         90        100        110        120 
LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR TRKKLEEERH TLITEMVALN 

       130        140        150        160        170        180 
PDFKPPADYK PPATRVSDKV MIPQDEYPEI NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK 

       190        200        210        220        230        240 
GSVKEGKVGR KDGQMLPGED EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR 

       250        260        270        280        290        300 
KMQLRELARL NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP 

       310        320        330        340        350        360 
QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA SNPPPPSLMS 

       370        380        390        400        410        420 
TTQSRPPWMN SGPSENRPYH GMHGGGPGGP GGGPHSFPHP LPSLTGGHGG HPMQHNPNGP 

       430        440        450        460        470        480 
PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP 

       490        500        510        520        530        540 
PPPPPSGQPP PPPSGPLPPW QQQQQQPPPP PPPSSSMASS TPLPWQQNTT TTTTSAGTGS 

       550        560        570        580        590        600 
IPPWQQQQAA AAASPGTPQM QGNPTMVPLP PGVQPPLPPG APPPPPCSIE CLLCLLSSPN 

       610        620        630        640        650 
SLCLSPNRAA RIPPRGSDGP SHESEDFPRP LVTLPGRQPQ QRPWWTGWFG KAA 

« Hide

Isoform CW17E [UniParc].

Checksum: 8965858D8C10D170
Show »

FASTA54859,657
Isoform 3 [UniParc].

Checksum: 20885630AB6292B1
Show »

FASTA63968,347

References

« Hide 'large scale' references
[1]"Enhanced expression in spleen macrophages of the mouse homolog to the human putative tumor suppressor gene ZFM1."
Wrehlke C., Schmitt-Wrede H.-P., Qiao Z.D., Wunderlich F.
DNA Cell Biol. 16:761-767(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS CW17 AND CW17E).
Strain: C57BL/10.
Tissue: Spleen.
[2]"Genomic organization of mouse gene zfp162 (mzfm)."
Wrehlke C., Wiedemeyer W.-R., Schmitt-Wrede H.-P., Mincheva A., Lichter P., Wunderlich F.
DNA Cell Biol. 18:419-428(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C57B1/10.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS CW17E AND 3).
Strain: C3H/He.
Tissue: Mammary tumor and Osteoblast.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80; SER-82 AND TYR-87, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X80159 mRNA. Translation: CAA56440.1.
X85802 mRNA. Translation: CAA59797.1.
Y08907 Genomic DNA. Translation: CAA70113.1.
Y12838 Genomic DNA. Translation: CAA73359.1.
AC127556 Genomic DNA. No translation available.
AC167245 Genomic DNA. No translation available.
BC009091 mRNA. Translation: AAH09091.1.
BC055370 mRNA. Translation: AAH55370.1.
UniGeneMm.256422.

3D structure databases

ProteinModelPortalQ64213.
SMRQ64213. Positions 1-255.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60453N.
IntActQ64213. 3 interactions.
MINTMINT-4134251.

PTM databases

PhosphoSiteQ64213.

Proteomic databases

PaxDbQ64213.
PRIDEQ64213.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000131252; ENSMUSP00000121309; ENSMUSG00000024949. [Q64213-3]

Organism-specific databases

MGIMGI:1095403. Sf1.

Phylogenomic databases

eggNOGCOG5176.
GeneTreeENSGT00550000074434.
HOGENOMHOG000237321.
HOVERGENHBG063318.
InParanoidQ64213.
OMACLSPNRA.
OrthoDBEOG7K0ZC4.
TreeFamTF319159.

Gene expression databases

ArrayExpressQ64213.
BgeeQ64213.
CleanExMM_SF1.
GenevestigatorQ64213.

Family and domain databases

Gene3D3.30.1370.10. 1 hit.
4.10.60.10. 1 hit.
InterProIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR001878. Znf_CCHC.
[Graphical view]
PfamPF00013. KH_1. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTSM00322. KH. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMSSF54791. SSF54791. 1 hit.
PROSITEPS50084. KH_TYPE_1. 1 hit.
PS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSF1. mouse.
PROQ64213.
SOURCESearch...

Entry information

Entry nameSF01_MOUSE
AccessionPrimary (citable) accession number: Q64213
Secondary accession number(s): E9QK02 expand/collapse secondary AC list , O08817, P70167, Q61454, Q921Z4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 119 of the entry and version 6 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot