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Q64213

- SF01_MOUSE

UniProt

Q64213 - SF01_MOUSE

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Protein

Splicing factor 1

Gene
Sf1, Zfm1, Zfp162
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri277 – 29620CCHC-typeAdd
BLAST

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. Leydig cell differentiation Source: MGI
  2. male sex determination Source: MGI
  3. mRNA processing Source: UniProtKB-KW
  4. negative regulation of smooth muscle cell proliferation Source: Ensembl
  5. regulation of steroid biosynthetic process Source: MGI
  6. regulation of transcription, DNA-templated Source: UniProtKB-KW
  7. RNA splicing Source: UniProtKB-KW
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 1
Alternative name(s):
CW17
Mammalian branch point-binding protein
Short name:
BBP
Short name:
mBBP
Transcription factor ZFM1
Short name:
mZFM
Zinc finger gene in MEN1 locus
Zinc finger protein 162
Gene namesi
Name:Sf1
Synonyms:Zfm1, Zfp162
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1095403. Sf1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: MGI
  3. spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 653652Splicing factor 1PRO_0000050130Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei20 – 201Phosphoserine; by PKG By similarity
Modified residuei80 – 801Phosphoserine3 Publications
Modified residuei82 – 821Phosphoserine3 Publications
Modified residuei87 – 871Phosphotyrosine1 Publication

Post-translational modificationi

Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ64213.
PaxDbiQ64213.
PRIDEiQ64213.

PTM databases

PhosphoSiteiQ64213.

Expressioni

Tissue specificityi

Detected at intermediate levels in spleen. Lower levels in heart, kidney, brain, liver, testis, bone marrow, adrenal gland, lymph nodes, pancreas and thymus.

Gene expression databases

ArrayExpressiQ64213.
BgeeiQ64213.
CleanExiMM_SF1.
GenevestigatoriQ64213.

Interactioni

Subunit structurei

Binds U2AF2. Interacts with U1 snRNA. Interacts with RBM17. Binds EWSR1, FUS and TAF15 By similarity.

Protein-protein interaction databases

DIPiDIP-60453N.
IntActiQ64213. 3 interactions.
MINTiMINT-4134251.

Structurei

3D structure databases

ProteinModelPortaliQ64213.
SMRiQ64213. Positions 1-255.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini141 – 22282KHAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi15 – 195Nuclear localization signal Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi324 – 585262Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the BBP/SF1 family.
Contains 1 KH domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri277 – 29620CCHC-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5176.
GeneTreeiENSGT00550000074434.
HOGENOMiHOG000237321.
HOVERGENiHBG063318.
InParanoidiQ64213.
OMAiCLSPNRA.
OrthoDBiEOG7K0ZC4.
TreeFamiTF319159.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform CW17 (identifier: Q64213-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER    50
AYIVQLQIED LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR 100
TRKKLEEERH TLITEMVALN PDFKPPADYK PPATRVSDKV MIPQDEYPEI 150
NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK GSVKEGKVGR KDGQMLPGED 200
EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR KMQLRELARL 250
NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP 300
QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA 350
SNPPPPSLMS TTQSRPPWMN SGPSENRPYH GMHGGGPGGP GGGPHSFPHP 400
LPSLTGGHGG HPMQHNPNGP PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY 450
LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP PPPPPSGQPP PPPSGPLPPW 500
QQQQQQPPPP PPPSSSMASS TPLPWQQNTT TTTTSAGTGS IPPWQQQQAA 550
AAASPGTPQM QGNPTMVPLP PGVQPPLPPG APPPPPCSIE CLLCLLSSPN 600
SLCLSPNRAA RIPPRGSDGP SHESEDFPRP LVTLPGRQPQ QRPWWTGWFG 650
KAA 653
Length:653
Mass (Da):70,403
Last modified:July 27, 2011 - v6
Checksum:i5C159302822509D1
GO
Isoform CW17E (identifier: Q64213-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     529-653: TTTTTTSAGT...WWTGWFGKAA → SLPAAAMARAMRVRTFRAHW

Show »
Length:548
Mass (Da):59,657
Checksum:i8965858D8C10D170
GO
Isoform 3 (identifier: Q64213-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     586-653: PCSIECLLCL...WWTGWFGKAA → PPPPPGSAGM...PAPPPPPPQN

Note: No experimental confirmation available.

Show »
Length:639
Mass (Da):68,347
Checksum:i20885630AB6292B1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei529 – 653125TTTTT…FGKAA → SLPAAAMARAMRVRTFRAHW in isoform CW17E. VSP_050424Add
BLAST
Alternative sequencei586 – 65368PCSIE…FGKAA → PPPPPGSAGMMYAPPPPPPP PMDPSNFVTMMGMGVAGMPP FGMPPAPPPPPPQN in isoform 3. VSP_008840Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1841K → E in CAA59797. 1 Publication
Sequence conflicti184 – 1841K → E in CAA73359. 1 Publication
Sequence conflicti509 – 5091P → S in AAH55370. 1 Publication
Sequence conflicti524 – 5241P → L in AAH09091. 1 Publication
Sequence conflicti586 – 5861P → T in CAA73359. 1 Publication
Sequence conflicti586 – 5861P → T in CAA70113. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X80159 mRNA. Translation: CAA56440.1.
X85802 mRNA. Translation: CAA59797.1.
Y08907 Genomic DNA. Translation: CAA70113.1.
Y12838 Genomic DNA. Translation: CAA73359.1.
AC127556 Genomic DNA. No translation available.
AC167245 Genomic DNA. No translation available.
BC009091 mRNA. Translation: AAH09091.1.
BC055370 mRNA. Translation: AAH55370.1.
CCDSiCCDS50368.1. [Q64213-3]
CCDS50369.1. [Q64213-2]
UniGeneiMm.256422.

Genome annotation databases

EnsembliENSMUST00000131252; ENSMUSP00000121309; ENSMUSG00000024949. [Q64213-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X80159 mRNA. Translation: CAA56440.1 .
X85802 mRNA. Translation: CAA59797.1 .
Y08907 Genomic DNA. Translation: CAA70113.1 .
Y12838 Genomic DNA. Translation: CAA73359.1 .
AC127556 Genomic DNA. No translation available.
AC167245 Genomic DNA. No translation available.
BC009091 mRNA. Translation: AAH09091.1 .
BC055370 mRNA. Translation: AAH55370.1 .
CCDSi CCDS50368.1. [Q64213-3 ]
CCDS50369.1. [Q64213-2 ]
UniGenei Mm.256422.

3D structure databases

ProteinModelPortali Q64213.
SMRi Q64213. Positions 1-255.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60453N.
IntActi Q64213. 3 interactions.
MINTi MINT-4134251.

PTM databases

PhosphoSitei Q64213.

Proteomic databases

MaxQBi Q64213.
PaxDbi Q64213.
PRIDEi Q64213.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000131252 ; ENSMUSP00000121309 ; ENSMUSG00000024949 . [Q64213-3 ]

Organism-specific databases

MGIi MGI:1095403. Sf1.

Phylogenomic databases

eggNOGi COG5176.
GeneTreei ENSGT00550000074434.
HOGENOMi HOG000237321.
HOVERGENi HBG063318.
InParanoidi Q64213.
OMAi CLSPNRA.
OrthoDBi EOG7K0ZC4.
TreeFami TF319159.

Miscellaneous databases

ChiTaRSi SF1. mouse.
PROi Q64213.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q64213.
Bgeei Q64213.
CleanExi MM_SF1.
Genevestigatori Q64213.

Family and domain databases

Gene3Di 3.30.1370.10. 1 hit.
4.10.60.10. 1 hit.
InterProi IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF00013. KH_1. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view ]
SMARTi SM00322. KH. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF54791. SSF54791. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50158. ZF_CCHC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Enhanced expression in spleen macrophages of the mouse homolog to the human putative tumor suppressor gene ZFM1."
    Wrehlke C., Schmitt-Wrede H.-P., Qiao Z.D., Wunderlich F.
    DNA Cell Biol. 16:761-767(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS CW17 AND CW17E).
    Strain: C57BL/10.
    Tissue: Spleen.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57B1/10.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS CW17E AND 3).
    Strain: C3H/He.
    Tissue: Mammary tumor and Osteoblast.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80; SER-82 AND TYR-87, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiSF01_MOUSE
AccessioniPrimary (citable) accession number: Q64213
Secondary accession number(s): E9QK02
, O08817, P70167, Q61454, Q921Z4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 122 of the entry and version 6 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi