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Protein

Peflin

Gene

Pef1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidences show that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (By similarity). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (PubMed:27276012).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi126 – 1371PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi193 – 2042PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Peflin1 Publication
Alternative name(s):
PEF protein with a long N-terminal hydrophobic domainBy similarity
Penta-EF hand domain-containing protein 1Imported
Gene namesi
Name:Pef1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1359536. Pef1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002470471 – 283PeflinAdd BLAST283

Post-translational modificationi

Ubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ641Z8.
PRIDEiQ641Z8.

Expressioni

Gene expression databases

BgeeiENSRNOG00000013972.
GenevisibleiQ641Z8. RN.

Interactioni

Subunit structurei

Heterodimer; heterodimerizes (via the EF-hand 5) with PDCD6. Dissociates from PDCD6 in presence of calcium.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018742.

Structurei

3D structure databases

ProteinModelPortaliQ641Z8.
SMRiQ641Z8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati21 – 2919
Repeati31 – 3929
Repeati41 – 4838
Repeati49 – 58410
Repeati59 – 6759
Repeati75 – 8369
Repeati84 – 9178
Repeati92 – 9988
Repeati100 – 10899
Domaini113 – 148EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini149 – 179EF-hand 2PROSITE-ProRule annotationAdd BLAST31
Domaini180 – 215EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini216 – 252EF-hand 4PROSITE-ProRule annotationAdd BLAST37
Domaini253 – 282EF-hand 5PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 1089 X 9 AA approximate tandem repeat of [AP]-P-G-G-P-Y-G-G-P-PAdd BLAST88
Regioni203 – 283Required for interaction with PDCD6By similarityAdd BLAST81

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0037. Eukaryota.
ENOG410YKQK. LUCA.
GeneTreeiENSGT00620000087734.
HOGENOMiHOG000231983.
HOVERGENiHBG004492.
InParanoidiQ641Z8.
OMAiPPQNYGF.
OrthoDBiEOG091G0ISY.
PhylomeDBiQ641Z8.
TreeFamiTF314682.

Family and domain databases

InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
PfamiView protein in Pfam
PF13202. EF-hand_5. 1 hit.
PF13405. EF-hand_6. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 3 hits.
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.

Sequencei

Sequence statusi: Complete.

Q641Z8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASYPDGQSY PGAAGQVPGP HPGGYYPGPP HGGGQYGSGF PPGGYGAPAP
60 70 80 90 100
GGPYGYPSAG GTPSGTPGGP YGGGPPGGPY GGGPPGGPYG QAHPSPYGTQ
110 120 130 140 150
PPGPYGQGGV PPNVDPEAYS WFQSVDADHS GYISLKELKQ ALVNSNWSSF
160 170 180 190 200
NDETCLMMIN MFDKTKTGRI DVVGFSALWK FLQQWKNLFQ QYDRDHSGSI
210 220 230 240 250
SSTELQQALS QMGYNLSPQF TQLLVSRYCT RSAIPAMQLD CFIKVCTQLQ
260 270 280
VLTEAFREKD TAVQGNIRLS FEDFVTMTAS RML
Length:283
Mass (Da):30,012
Last modified:October 25, 2004 - v1
Checksum:i2454D005C02C595D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082028 mRNA. Translation: AAH82028.1.
RefSeqiNP_001007652.1. NM_001007651.1.
UniGeneiRn.96097.

Genome annotation databases

EnsembliENSRNOT00000018742; ENSRNOP00000018742; ENSRNOG00000013972.
GeneIDi297900.
KEGGirno:297900.

Similar proteinsi

Entry informationi

Entry nameiPEF1_RAT
AccessioniPrimary (citable) accession number: Q641Z8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: October 25, 2004
Last modified: August 30, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome