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Protein

EH domain-containing protein 1

Gene

Ehd1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes (By similarity). Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes (By similarity). Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth (PubMed:23572513). Plays a role in myoblast fusion (By similarity). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (By similarity). Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei220 – 2201ATPBy similarity
Binding sitei258 – 2581ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPBy similarity
Calcium bindingi489 – 50012PROSITE-ProRule annotationBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
EH domain-containing protein 1Curated
Gene namesi
Name:Ehd1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1309017. Ehd1.

Subcellular locationi

  • Recycling endosome membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side Curated
  • Early endosome membrane By similarity; Peripheral membrane protein Curated; Cytoplasmic side Curated
  • Cell membrane By similarity; Peripheral membrane protein Curated; Cytoplasmic side Curated
  • Cell projectioncilium membrane By similarity; Peripheral membrane protein Curated; Cytoplasmic side Curated

  • Note: Preferentially associates with tubular recycling endosomes (By similarity). Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes (By similarity). Localizes to the ciliary pocket from where the cilium protrudes (By similarity). Colocalizes with BACE1 in tubulovesicular cytoplasmic membranes. Colocalizes with BACE1 and APP amyloid beta proteins in hippocampal mossy fiber terminals (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 534534EH domain-containing protein 1PRO_0000306858Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei355 – 3551PhosphoserineBy similarity
Modified residuei456 – 4561PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ641Z6.
PRIDEiQ641Z6.

PTM databases

iPTMnetiQ641Z6.

Expressioni

Gene expression databases

GenevisibleiQ641Z6. RN.

Interactioni

Subunit structurei

Homooligomer, and heterooligomer with EHD2, EHD3 and EHD4, ATP-binding is required for heterooligomerization (By similarity). Interacts (via EH domain) with MICALL1 (via NPF1 motif); the interaction is direct and recruits EHD1 to membranes (PubMed:23572513). Interacts with RAB35; the interaction is indirect through MICALL1 and recruits EHD1 to membranes (PubMed:23572513). Interacts (via EH domain) with PACSIN2 (via NPF motifs); regulates localization to tubular recycling endosome membranes (PubMed:15930129). Interacts with PACSIN1 (PubMed:15930129). Interacts with RAB8A (By similarity). Interacts with FER1L5 (via second C2 domain) (By similarity). Interacts with MYOF (By similarity). Interacts with ZFYVE20 (By similarity). Interacts (via EH domain) with RAB11FIP2 (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Pacsin2Q9QY172EBI-492911,EBI-491201
Snap29Q9Z2P62EBI-492911,EBI-492883

Protein-protein interaction databases

BioGridi254403. 2 interactions.
IntActiQ641Z6. 2 interactions.
STRINGi10116.ENSRNOP00000051714.

Structurei

3D structure databases

ProteinModelPortaliQ641Z6.
SMRiQ641Z6. Positions 1-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 286232Dynamin-type GAdd
BLAST
Domaini444 – 53289EHPROSITE-ProRule annotationAdd
BLAST
Domaini476 – 51136EF-handPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili198 – 22730Sequence analysisAdd
BLAST

Domaini

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.By similarity

Sequence similaritiesi

Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 EH domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1954. Eukaryota.
ENOG410XYGB. LUCA.
GeneTreeiENSGT00760000118985.
HOGENOMiHOG000242040.
HOVERGENiHBG018183.
InParanoidiQ641Z6.
KOiK12483.
OMAiRFMCAQM.
OrthoDBiEOG757CX9.
PhylomeDBiQ641Z6.
TreeFamiTF314429.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR022812. Dynamin_SF.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
IPR029951. EHD1/EHD3.
IPR031692. EHD_N.
IPR030381. G_DYNAMIN_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11216:SF67. PTHR11216:SF67. 1 hit.
PfamiPF00350. Dynamin_N. 1 hit.
PF12763. EF-hand_4. 1 hit.
PF16880. EHD_N. 1 hit.
[Graphical view]
SMARTiSM00027. EH. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q641Z6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSWVSKDAR RKKEPELFQT VAEGLRQLYA QKLLPLEEHY RFHEFHSPAL
60 70 80 90 100
EDADFDNKPM VLLVGQYSTG KTTFIRHLIE QDFPGMRIGP EPTTDSFIAV
110 120 130 140 150
MHGPTEGVVP GNALVVDPRR PFRKLNAFGN AFLNRFMCAQ LPNPVLDSIS
160 170 180 190 200
IIDTPGILSG EKQRISRGYD FAAVLEWFAE RVDRIILLFD AHKLDISDEF
210 220 230 240 250
SEVIKALKNH EDKIRVVLNK ADQIETQQLM RVYGALMWSL GKIINTPEVV
260 270 280 290 300
RVYIGSFWSH PLLIPDNRKL FEAEEQDLFK DIQSLPRNAA LRKLNDLIKR
310 320 330 340 350
ARLAKVHAYI ISSLKKEMPN VFGKESKKKE LVNNLGEIYQ KIEREHQISS
360 370 380 390 400
GDFPSLRKMQ ELLQTQDFSK FQALKPKLLD TVDDMLANDI ARLMVMVRQE
410 420 430 440 450
ESLMPSQAVK GGAFDGTMNG PFGHGYGEGA GEGIDDVEWV VGKDKPTYDE
460 470 480 490 500
IFYTLSPVNG KITGANAKKE MVKSKLPNTV LGKIWKLADV DKDGLLDDEE
510 520 530
FALANHLIKV KLEGHELPAD LPPHLIPPSK RRHE
Length:534
Mass (Da):60,603
Last modified:October 25, 2004 - v1
Checksum:i35502055BC6164F7
GO

Sequence cautioni

The sequence AAI60908.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082030 mRNA. Translation: AAH82030.1.
BC160908 mRNA. Translation: AAI60908.1. Different initiation.
RefSeqiNP_001011939.1. NM_001011939.2.
UniGeneiRn.8141.

Genome annotation databases

EnsembliENSRNOT00000054830; ENSRNOP00000051714; ENSRNOG00000043503.
GeneIDi293692.
KEGGirno:293692.
UCSCiRGD:1309017. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082030 mRNA. Translation: AAH82030.1.
BC160908 mRNA. Translation: AAI60908.1. Different initiation.
RefSeqiNP_001011939.1. NM_001011939.2.
UniGeneiRn.8141.

3D structure databases

ProteinModelPortaliQ641Z6.
SMRiQ641Z6. Positions 1-534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi254403. 2 interactions.
IntActiQ641Z6. 2 interactions.
STRINGi10116.ENSRNOP00000051714.

PTM databases

iPTMnetiQ641Z6.

Proteomic databases

PaxDbiQ641Z6.
PRIDEiQ641Z6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000054830; ENSRNOP00000051714; ENSRNOG00000043503.
GeneIDi293692.
KEGGirno:293692.
UCSCiRGD:1309017. rat.

Organism-specific databases

CTDi10938.
RGDi1309017. Ehd1.

Phylogenomic databases

eggNOGiKOG1954. Eukaryota.
ENOG410XYGB. LUCA.
GeneTreeiENSGT00760000118985.
HOGENOMiHOG000242040.
HOVERGENiHBG018183.
InParanoidiQ641Z6.
KOiK12483.
OMAiRFMCAQM.
OrthoDBiEOG757CX9.
PhylomeDBiQ641Z6.
TreeFamiTF314429.

Enzyme and pathway databases

ReactomeiR-RNO-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

PROiQ641Z6.

Gene expression databases

GenevisibleiQ641Z6. RN.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR022812. Dynamin_SF.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
IPR029951. EHD1/EHD3.
IPR031692. EHD_N.
IPR030381. G_DYNAMIN_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11216:SF67. PTHR11216:SF67. 1 hit.
PfamiPF00350. Dynamin_N. 1 hit.
PF12763. EF-hand_4. 1 hit.
PF16880. EHD_N. 1 hit.
[Graphical view]
SMARTiSM00027. EH. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Brown Norway.
    Tissue: Kidney and Testis.
  2. "EHD proteins associate with syndapin I and II and such interactions play a crucial role in endosomal recycling."
    Braun A., Pinyol R., Dahlhaus R., Koch D., Fonarev P., Grant B.D., Kessels M.M., Qualmann B.
    Mol. Biol. Cell 16:3642-3658(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PACSIN1 AND PACSIN2.
  3. Maurya D.K., Bhargava P.
    Submitted (JAN-2009) to UniProtKB
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth."
    Kobayashi H., Fukuda M.
    J. Cell Sci. 126:2424-2435(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NEURITE OUTGROWTH, SUBCELLULAR LOCATION, INTERACTION WITH MICALL1 AND RAB35.

Entry informationi

Entry nameiEHD1_RAT
AccessioniPrimary (citable) accession number: Q641Z6
Secondary accession number(s): B1H289
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 25, 2004
Last modified: June 8, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.