Reviewed, UniProtKB/Swiss-Prot Q641Z4 (CDK9_RAT)

Names and origin

Protein names

Recommended name:
    Cell division protein kinase 9
    EC=2.7.11.22
    EC=2.7.11.23
Alternative name(s):
    Cyclin-dependent kinase 9

Gene names

Name:

Cdk9

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subunit structure	Associates with CCNT1/cyclin-T1 to form P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Also associates with CCNK/cyclin-K. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Cell division protein kinase 9		PRO_0000085802
Regions
	Domain	19 – 315	297	Protein kinase
	Nucleotide binding	25 – 33	9	ATP By similarity
Sites
	Active site	149	1	Proton acceptor By similarity
	Binding site	48	1	ATP By similarity
Amino acid modifications
	Modified residue	185	1	Phosphotyrosine By similarity
	Modified residue	186	1	Phosphothreonine By similarity
	Modified residue	347	1	Phosphoserine By similarity
	Modified residue	350	1	Phosphothreonine By similarity
	Modified residue	353	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q641Z4-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 973B18869F9963E3	FASTA	372	42,762
	10 20 30 40 50 60 MAKQYDSVEC PFCDEVTKYE KLAKIGQGTF GEVFKAKHRQ TGQKVALKKV LMENEKEGFP 70 80 90 100 110 120 ITALREIKIL QLLKHENVVN LIEICRTKAS PYNRCKGSIY LVFDFCEHDL AGLLSNVLVK 130 140 150 160 170 180 FTLSEIKRVM QMLLNGLYYI HRNKILHRDM KAANVLITRD GVLKLADFGL ARAFSLAKNS 190 200 210 220 230 240 QPNRYTNRVV TLWYRPPELL LGERDYGPPI DLWGAGCIMA EMWTRSPIMQ GNTEQHQLAL 250 260 270 280 290 300 ISQLCGSITP EVWPNVDKYE LFEKLELVKG QKRKVKDRLK AYVRDPYALD LIDKLLVLDP 310 320 330 340 350 360 AQRIDSDDAL NHDFFWSDPM PSDLKGMLST HLTSMFEYLA PPRRKGSQIT QQSTNQSRNP 370 ATTNQTEFER VF

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC082037 mRNA. Translation: AAH82037.1.
IPI	IPI00373595.
RefSeq	NP_001007744.1.
UniGene	Rn.98228
3D structure databases
SMR	Q641Z4. Positions 7-326.
ModBase	Search...
Protein-protein interaction databases
STRING	Q641Z4.
PTM databases
PhosphoSite	Q641Z4.
Genome annotation databases
Ensembl	ENSRNOT00000032893; ENSRNOP00000037778; ENSRNOG00000022586; Rattus norvegicus. [Genome view]
GeneID	362110.
KEGG	rno:362110.
UCSC	NM_001007743. rat.
Organism-specific databases
CTD	362110.
RGD	1359638. Cdk9.
Phylogenomic databases
eggNOG	roNOG10985.
HOVERGEN	Q641Z4.
InParanoid	Q641Z4.
OMA	FKARHRK.
PhylomeDB	Q641Z4.
Enzyme and pathway databases
BRENDA	2.7.11.22. 248. 2.7.11.23. 248.
Gene expression databases
ArrayExpress	Q641Z4.
Genevestigator	Q641Z4.
GermOnline	ENSRNOG00000022586. Rattus norvegicus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	678687.

Entry information

Entry name

CDK9_RAT

Accession

Primary (citable) accession number: Q641Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	October 25, 2004
Last modified:	February 9, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)