ID   ATG7_RAT                Reviewed;         698 AA.
AC   Q641Y5;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   14-DEC-2011, entry version 60.
DE   RecName: Full=Ubiquitin-like modifier-activating enzyme ATG7;
DE   AltName: Full=ATG12-activating enzyme E1 ATG7;
DE   AltName: Full=Autophagy-related protein 7;
DE            Short=APG7-like;
GN   Name=Atg7; Synonyms=Apg7l;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   TISSUE SPECIFICITY.
RX   MEDLINE=21888283; PubMed=11890701; DOI=10.1006/bbrc.2002.6645;
RA   Tanida I., Tanida-Miyake E., Nishitani T., Komatsu M., Yamazaki H.,
RA   Ueno T., Kominami E.;
RT   "Murine Apg12p has a substrate preference for murine Apg7p over three
RT   Apg8p homologs.";
RL   Biochem. Biophys. Res. Commun. 292:256-262(2002).
CC   -!- FUNCTION: Functions as an E1 enzyme essential for multisubstrates
CC       such as ATG8-like proteins and ATG12. Forms intermediate
CC       conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2
CC       or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential
CC       for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to
CC       ATG5 and ATG3 (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- TISSUE SPECIFICITY: Widely expressed.
CC   -!- DOMAIN: The C-terminal part of the protein is essential for the
CC       dimerization and interaction with ATG3 and ATG12 (By similarity).
CC   -!- SIMILARITY: Belongs to the ATG7 family.
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DR   EMBL; BC082059; AAH82059.1; -; mRNA.
DR   IPI; IPI00471650; -.
DR   RefSeq; NP_001012097.1; NM_001012097.1.
DR   UniGene; Rn.162765; -.
DR   ProteinModelPortal; Q641Y5; -.
DR   STRING; Q641Y5; -.
DR   PRIDE; Q641Y5; -.
DR   Ensembl; ENSRNOT00000067532; ENSRNOP00000063617; ENSRNOG00000007486.
DR   GeneID; 312647; -.
DR   KEGG; rno:312647; -.
DR   UCSC; NM_001012097; rat.
DR   CTD; 10533; -.
DR   RGD; 1304817; Atg7.
DR   eggNOG; roNOG09556; -.
DR   GeneTree; ENSGT00390000017509; -.
DR   HOVERGEN; HBG080877; -.
DR   InParanoid; Q641Y5; -.
DR   OrthoDB; EOG43N7C8; -.
DR   PhylomeDB; Q641Y5; -.
DR   NextBio; 665058; -.
DR   Genevestigator; Q641Y5; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019778; F:APG12 activating enzyme activity; ISS:UniProtKB.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006914; P:autophagy; IEP:RGD.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR006285; E1-like_Apg7.
DR   InterPro; IPR009036; Molybdenum_cofac_synth_MoeB.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 2.
DR   KO; K08337; -.
DR   Pfam; PF00899; ThiF; 1.
DR   SUPFAM; SSF69572; MoeB; 1.
DR   TIGRFAMs; TIGR01381; E1_like_apg7; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Autophagy; Complete proteome; Cytoplasm;
KW   Protein transport; Reference proteome; Transport;
KW   Ubl conjugation pathway.
FT   CHAIN         1    698       Ubiquitin-like modifier-activating enzyme
FT                                ATG7.
FT                                /FTId=PRO_0000212808.
FT   ACT_SITE    567    567       Glycyl thioester intermediate (By
FT                                similarity).
FT   MOD_RES     302    302       N6-acetyllysine (By similarity).
SQ   SEQUENCE   698 AA;  77436 MW;  B26001CB18054498 CRC64;
     MGDPGLSKLQ FAPFNSALDV GFWHELTQKK LNEYRLDEAP KDIKGYYYNG DSAGLPTRLT
     LEFSAFDMSA PTPARCCPAM GTLHNTNTLE AFKTADKKLL LEQSANEIWE AIKSGAALEN
     PMLLNKFLLL TFADLKKYHF YYWFCCPALC LPESIPLIRG PVGLDQRLSP KQIQALEHAY
     DDLCRTEGVT ALPYFLFKYD DDTVLVSLLK HYSDFFQGQR TKLTVGVYDP CNLTQHPGWP
     LRNFLVLAAH RWSGSFQSVE VLCFRDRTMQ GARDVTHSII FEVKLPEMAF SPDCPKAVGW
     EKNQKGGMGP RMVNLSGCMD PKRLAESSVD LNLKLMCWRL VPTLDLDKVV SVKCLLLGAG
     TLGCNVARTL MGWGVRHVTF VDNAKISYSN PVRQPLYEFE DCLGGGKPKA LAAAERLQKI
     FPGVNASGFN MSIPMPGHPV NFSDVTMEQA RRDVEQLEEL IDSHDVIFLL MDTRESRWLP
     TVIAASKRKL VINAALGFDT FVVMRHGLKK PKQQGAGDLC PSHLVAPADL GSSLFANIPG
     YKLGCYFCND VVAPGDSTRD RTLDQQCTVS RPGLAVIAGA LAVELMVSVL QHPEGGYAIA
     SSSDDRMNEP PTSLGLVPHQ IRGFLSRFDN VLPVSLAFDK CTACSSKVLD QYEQEGFTFL
     AKVFNSSHSF LEDLTGLTLL HQETQAAEIW DMSDEETV
//