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Protein

Ubiquitin-like modifier-activating enzyme ATG7

Gene

Atg7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei567Glycyl thioester intermediateBy similarity1

GO - Molecular functioni

  • Atg12 activating enzyme activity Source: UniProtKB
  • Atg8 activating enzyme activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-1632852. Macroautophagy.
R-RNO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like modifier-activating enzyme ATG7
Alternative name(s):
ATG12-activating enzyme E1 ATG7
Autophagy-related protein 7
Short name:
APG7-like
Gene namesi
Name:Atg7
Synonyms:Apg7l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1304817. Atg7.

Subcellular locationi

  • Cytoplasm By similarity
  • Preautophagosomal structure By similarity

  • Note: Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002128081 – 698Ubiquitin-like modifier-activating enzyme ATG7Add BLAST698

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei693PhosphoserineBy similarity1

Post-translational modificationi

Acetylated by EP300.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ641Y5.
PRIDEiQ641Y5.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSRNOG00000007486.
GenevisibleiQ641Y5. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Forms intermediate conjugates with ATG8 family proteins such as GABARAP, GABARAPL1, GABARAPL2, MAP1LC3A, or GABARAPL1. Interacts with EP300 acetyltransferase and FOXO1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063617.

Structurei

3D structure databases

ProteinModelPortaliQ641Y5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 13FAP motif3

Domaini

The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.By similarity
The N-terminal FAP motif (residues 11 to 13) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.By similarity

Sequence similaritiesi

Belongs to the ATG7 family.Curated

Phylogenomic databases

eggNOGiKOG2337. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00390000017509.
HOGENOMiHOG000162379.
HOVERGENiHBG080877.
InParanoidiQ641Y5.
KOiK08337.
OMAiVMAIPMP.
OrthoDBiEOG091G0628.
PhylomeDBiQ641Y5.
TreeFamiTF105689.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006285. Atg7.
IPR032197. Atg7_N.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PANTHERiPTHR10953:SF3. PTHR10953:SF3. 2 hits.
PfamiPF16420. ATG7_N. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 2 hits.
TIGRFAMsiTIGR01381. E1_like_apg7. 1 hit.

Sequencei

Sequence statusi: Complete.

Q641Y5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDPGLSKLQ FAPFNSALDV GFWHELTQKK LNEYRLDEAP KDIKGYYYNG
60 70 80 90 100
DSAGLPTRLT LEFSAFDMSA PTPARCCPAM GTLHNTNTLE AFKTADKKLL
110 120 130 140 150
LEQSANEIWE AIKSGAALEN PMLLNKFLLL TFADLKKYHF YYWFCCPALC
160 170 180 190 200
LPESIPLIRG PVGLDQRLSP KQIQALEHAY DDLCRTEGVT ALPYFLFKYD
210 220 230 240 250
DDTVLVSLLK HYSDFFQGQR TKLTVGVYDP CNLTQHPGWP LRNFLVLAAH
260 270 280 290 300
RWSGSFQSVE VLCFRDRTMQ GARDVTHSII FEVKLPEMAF SPDCPKAVGW
310 320 330 340 350
EKNQKGGMGP RMVNLSGCMD PKRLAESSVD LNLKLMCWRL VPTLDLDKVV
360 370 380 390 400
SVKCLLLGAG TLGCNVARTL MGWGVRHVTF VDNAKISYSN PVRQPLYEFE
410 420 430 440 450
DCLGGGKPKA LAAAERLQKI FPGVNASGFN MSIPMPGHPV NFSDVTMEQA
460 470 480 490 500
RRDVEQLEEL IDSHDVIFLL MDTRESRWLP TVIAASKRKL VINAALGFDT
510 520 530 540 550
FVVMRHGLKK PKQQGAGDLC PSHLVAPADL GSSLFANIPG YKLGCYFCND
560 570 580 590 600
VVAPGDSTRD RTLDQQCTVS RPGLAVIAGA LAVELMVSVL QHPEGGYAIA
610 620 630 640 650
SSSDDRMNEP PTSLGLVPHQ IRGFLSRFDN VLPVSLAFDK CTACSSKVLD
660 670 680 690
QYEQEGFTFL AKVFNSSHSF LEDLTGLTLL HQETQAAEIW DMSDEETV
Length:698
Mass (Da):77,436
Last modified:October 25, 2004 - v1
Checksum:iB26001CB18054498
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082059 mRNA. Translation: AAH82059.1.
RefSeqiNP_001012097.1. NM_001012097.1.
XP_006237095.1. XM_006237033.3.
UniGeneiRn.162765.

Genome annotation databases

EnsembliENSRNOT00000067532; ENSRNOP00000063617; ENSRNOG00000007486.
GeneIDi312647.
KEGGirno:312647.
UCSCiRGD:1304817. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082059 mRNA. Translation: AAH82059.1.
RefSeqiNP_001012097.1. NM_001012097.1.
XP_006237095.1. XM_006237033.3.
UniGeneiRn.162765.

3D structure databases

ProteinModelPortaliQ641Y5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063617.

Proteomic databases

PaxDbiQ641Y5.
PRIDEiQ641Y5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000067532; ENSRNOP00000063617; ENSRNOG00000007486.
GeneIDi312647.
KEGGirno:312647.
UCSCiRGD:1304817. rat.

Organism-specific databases

CTDi10533.
RGDi1304817. Atg7.

Phylogenomic databases

eggNOGiKOG2337. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00390000017509.
HOGENOMiHOG000162379.
HOVERGENiHBG080877.
InParanoidiQ641Y5.
KOiK08337.
OMAiVMAIPMP.
OrthoDBiEOG091G0628.
PhylomeDBiQ641Y5.
TreeFamiTF105689.

Enzyme and pathway databases

ReactomeiR-RNO-1632852. Macroautophagy.
R-RNO-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ641Y5.

Gene expression databases

BgeeiENSRNOG00000007486.
GenevisibleiQ641Y5. RN.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006285. Atg7.
IPR032197. Atg7_N.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PANTHERiPTHR10953:SF3. PTHR10953:SF3. 2 hits.
PfamiPF16420. ATG7_N. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 2 hits.
TIGRFAMsiTIGR01381. E1_like_apg7. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiATG7_RAT
AccessioniPrimary (citable) accession number: Q641Y5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 25, 2004
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.