Q641Y5 (ATG7_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-like modifier-activating enzyme ATG7

Alternative name(s):
ATG12-activating enzyme E1 ATG7
Autophagy-related protein 7
Short name=APG7-like

Gene names

Name:	Atg7
Synonyms:	Apg7l

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	698 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3 By similarity.
Subcellular location	Cytoplasm Probable.
Tissue specificity	Widely expressed. Ref.2
Domain	The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12 By similarity.
Sequence similarities	Belongs to the ATG7 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport Ubl conjugation pathway
Cellular component	Cytoplasm
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from expression pattern. Source: RGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	APG12 activating enzyme activity Inferred from sequence or structural similarity. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 698	698	Ubiquitin-like modifier-activating enzyme ATG7		PRO_0000212808
Sites
	Active site	567	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	302	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q641Y5 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: B26001CB18054498	FASTA	698	77,436
	10 20 30 40 50 60 MGDPGLSKLQ FAPFNSALDV GFWHELTQKK LNEYRLDEAP KDIKGYYYNG DSAGLPTRLT 70 80 90 100 110 120 LEFSAFDMSA PTPARCCPAM GTLHNTNTLE AFKTADKKLL LEQSANEIWE AIKSGAALEN 130 140 150 160 170 180 PMLLNKFLLL TFADLKKYHF YYWFCCPALC LPESIPLIRG PVGLDQRLSP KQIQALEHAY 190 200 210 220 230 240 DDLCRTEGVT ALPYFLFKYD DDTVLVSLLK HYSDFFQGQR TKLTVGVYDP CNLTQHPGWP 250 260 270 280 290 300 LRNFLVLAAH RWSGSFQSVE VLCFRDRTMQ GARDVTHSII FEVKLPEMAF SPDCPKAVGW 310 320 330 340 350 360 EKNQKGGMGP RMVNLSGCMD PKRLAESSVD LNLKLMCWRL VPTLDLDKVV SVKCLLLGAG 370 380 390 400 410 420 TLGCNVARTL MGWGVRHVTF VDNAKISYSN PVRQPLYEFE DCLGGGKPKA LAAAERLQKI 430 440 450 460 470 480 FPGVNASGFN MSIPMPGHPV NFSDVTMEQA RRDVEQLEEL IDSHDVIFLL MDTRESRWLP 490 500 510 520 530 540 TVIAASKRKL VINAALGFDT FVVMRHGLKK PKQQGAGDLC PSHLVAPADL GSSLFANIPG 550 560 570 580 590 600 YKLGCYFCND VVAPGDSTRD RTLDQQCTVS RPGLAVIAGA LAVELMVSVL QHPEGGYAIA 610 620 630 640 650 660 SSSDDRMNEP PTSLGLVPHQ IRGFLSRFDN VLPVSLAFDK CTACSSKVLD QYEQEGFTFL 670 680 690 AKVFNSSHSF LEDLTGLTLL HQETQAAEIW DMSDEETV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs." Tanida I., Tanida-Miyake E., Nishitani T., Komatsu M., Yamazaki H., Ueno T., Kominami E. Biochem. Biophys. Res. Commun. 292:256-262(2002) [PubMed: 11890701] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC082059 mRNA. Translation: AAH82059.1.
IPI	IPI00471650.
RefSeq	NP_001012097.1. NM_001012097.1.
UniGene	Rn.162765.
3D structure databases
ProteinModelPortal	Q641Y5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q641Y5.
Proteomic databases
PRIDE	Q641Y5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000067532; ENSRNOP00000063617; ENSRNOG00000007486.
GeneID	312647.
KEGG	rno:312647.
UCSC	NM_001012097. rat.
Organism-specific databases
CTD	10533.
RGD	1304817. Atg7.
Phylogenomic databases
eggNOG	roNOG09556.
GeneTree	ENSGT00390000017509.
HOVERGEN	HBG080877.
InParanoid	Q641Y5.
OrthoDB	EOG43N7C8.
PhylomeDB	Q641Y5.
Gene expression databases
Genevestigator	Q641Y5.
Family and domain databases
InterPro	IPR006285. E1-like_Apg7. IPR009036. Molybdenum_cofac_synth_MoeB. IPR016040. NAD(P)-bd_dom. IPR000594. ThiF_NAD_FAD-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KO	K08337.
Pfam	PF00899. ThiF. 1 hit. [Graphical view]
SUPFAM	SSF69572. MoeB. 1 hit.
TIGRFAMs	TIGR01381. E1_like_apg7. 1 hit.
ProtoNet	Search...
Other
NextBio	665058.

Entry information

Entry name

ATG7_RAT

Accession

Primary (citable) accession number: Q641Y5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	October 25, 2004
Last modified:	December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program