Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin-like modifier-activating enzyme ATG7

Gene

Atg7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei567Glycyl thioester intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • autophagosome assembly Source: GO_Central
  • autophagy Source: GO_Central
  • autophagy of host cells involved in interaction with symbiont Source: Ensembl
  • autophagy of mitochondrion Source: GO_Central
  • cellular response to hyperoxia Source: UniProtKB
  • cellular response to morphine Source: RGD
  • cellular response to nitrogen starvation Source: GO_Central
  • cellular response to starvation Source: UniProtKB
  • C-terminal protein lipidation Source: GO_Central
  • defense response to virus Source: Ensembl
  • late nucleophagy Source: GO_Central
  • negative regulation of cell death Source: RGD
  • negative regulation of mitochondrial DNA replication Source: RGD
  • negative regulation of oxidative stress-induced neuron death Source: RGD
  • piecemeal microautophagy of the nucleus Source: GO_Central
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of autophagy Source: RGD
  • positive regulation of protein catabolic process Source: UniProtKB
  • positive regulation of protein modification process Source: Ensembl
  • protein transport Source: UniProtKB-KW
  • regulation of neuron death Source: RGD
  • response to fluoride Source: RGD
  • response to glucose Source: RGD
  • response to nutrient levels Source: RGD
  • suppression by virus of host autophagy Source: Ensembl

Keywordsi

Biological processAutophagy, Protein transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-1632852 Macroautophagy
R-RNO-6798695 Neutrophil degranulation
R-RNO-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like modifier-activating enzyme ATG7
Alternative name(s):
ATG12-activating enzyme E1 ATG7
Autophagy-related protein 7
Short name:
APG7-like
Gene namesi
Name:Atg7
Synonyms:Apg7l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1304817 Atg7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002128081 – 698Ubiquitin-like modifier-activating enzyme ATG7Add BLAST698

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei693PhosphoserineBy similarity1

Post-translational modificationi

Acetylated by EP300.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ641Y5
PRIDEiQ641Y5

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSRNOG00000007486
GenevisibleiQ641Y5 RN

Interactioni

Subunit structurei

Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Forms intermediate conjugates with ATG8 family proteins such as GABARAP, GABARAPL1, GABARAPL2, MAP1LC3A, or GABARAPL1. Interacts with EP300 acetyltransferase and FOXO1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063617

Structurei

3D structure databases

ProteinModelPortaliQ641Y5
SMRiQ641Y5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 13FAP motif3

Domaini

The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.By similarity
The N-terminal FAP motif (residues 11 to 13) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.By similarity

Sequence similaritiesi

Belongs to the ATG7 family.Curated

Phylogenomic databases

eggNOGiKOG2337 Eukaryota
COG0476 LUCA
GeneTreeiENSGT00390000017509
HOGENOMiHOG000162379
HOVERGENiHBG080877
InParanoidiQ641Y5
KOiK08337
OMAiCPKAVGW
OrthoDBiEOG091G0628
PhylomeDBiQ641Y5
TreeFamiTF105689

Family and domain databases

InterProiView protein in InterPro
IPR006285 Atg7
IPR032197 Atg7_N
IPR000594 ThiF_NAD_FAD-bd
IPR035985 Ubiquitin-activating_enz
PANTHERiPTHR10953:SF3 PTHR10953:SF3, 1 hit
PfamiView protein in Pfam
PF16420 ATG7_N, 1 hit
PF00899 ThiF, 1 hit
SUPFAMiSSF69572 SSF69572, 2 hits
TIGRFAMsiTIGR01381 E1_like_apg7, 1 hit

Sequencei

Sequence statusi: Complete.

Q641Y5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDPGLSKLQ FAPFNSALDV GFWHELTQKK LNEYRLDEAP KDIKGYYYNG
60 70 80 90 100
DSAGLPTRLT LEFSAFDMSA PTPARCCPAM GTLHNTNTLE AFKTADKKLL
110 120 130 140 150
LEQSANEIWE AIKSGAALEN PMLLNKFLLL TFADLKKYHF YYWFCCPALC
160 170 180 190 200
LPESIPLIRG PVGLDQRLSP KQIQALEHAY DDLCRTEGVT ALPYFLFKYD
210 220 230 240 250
DDTVLVSLLK HYSDFFQGQR TKLTVGVYDP CNLTQHPGWP LRNFLVLAAH
260 270 280 290 300
RWSGSFQSVE VLCFRDRTMQ GARDVTHSII FEVKLPEMAF SPDCPKAVGW
310 320 330 340 350
EKNQKGGMGP RMVNLSGCMD PKRLAESSVD LNLKLMCWRL VPTLDLDKVV
360 370 380 390 400
SVKCLLLGAG TLGCNVARTL MGWGVRHVTF VDNAKISYSN PVRQPLYEFE
410 420 430 440 450
DCLGGGKPKA LAAAERLQKI FPGVNASGFN MSIPMPGHPV NFSDVTMEQA
460 470 480 490 500
RRDVEQLEEL IDSHDVIFLL MDTRESRWLP TVIAASKRKL VINAALGFDT
510 520 530 540 550
FVVMRHGLKK PKQQGAGDLC PSHLVAPADL GSSLFANIPG YKLGCYFCND
560 570 580 590 600
VVAPGDSTRD RTLDQQCTVS RPGLAVIAGA LAVELMVSVL QHPEGGYAIA
610 620 630 640 650
SSSDDRMNEP PTSLGLVPHQ IRGFLSRFDN VLPVSLAFDK CTACSSKVLD
660 670 680 690
QYEQEGFTFL AKVFNSSHSF LEDLTGLTLL HQETQAAEIW DMSDEETV
Length:698
Mass (Da):77,436
Last modified:October 25, 2004 - v1
Checksum:iB26001CB18054498
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082059 mRNA Translation: AAH82059.1
RefSeqiNP_001012097.1, NM_001012097.1
XP_006237095.1, XM_006237033.3
UniGeneiRn.162765

Genome annotation databases

EnsembliENSRNOT00000067532; ENSRNOP00000063617; ENSRNOG00000007486
GeneIDi312647
KEGGirno:312647
UCSCiRGD:1304817 rat

Similar proteinsi

Entry informationi

Entry nameiATG7_RAT
AccessioniPrimary (citable) accession number: Q641Y5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 25, 2004
Last modified: May 23, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health