NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial precursor

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Q641Y2 (NDUS2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 64. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-49kD
Short name=CI-49kD
NADH-ubiquinone oxidoreductase 49 kDa subunit

Gene names

Name:

Ndufs2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. UniProtKB P17694
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. UniProtKB P17694 NADH + acceptor = NAD⁺ + reduced acceptor. UniProtKB P17694
Cofactor	Binds 1 4Fe-4S cluster.
Subunit structure	Complex I is composed of 45 different subunits. Component of the iron-sulfur (IP) fragment of the enzyme By similarity. Interacts with NDUFAF3 By similarity. UniProtKB P17694
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side Ref.2.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrial respiratory chain complex I Inferred from electronic annotation. Source: Compara
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 33

Mitochondrion By similarity UniProtKB P17694

Chain

34 – 463

430

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial UniProtKB P17694

PRO_0000342702

Sites

Metal binding

326

Iron-sulfur (4Fe-4S) Potential

Metal binding

332

Iron-sulfur (4Fe-4S) Potential

Metal binding

347

Iron-sulfur (4Fe-4S) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q641Y2 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: B9919E581C229C19
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FASTA

463

52,562

        10         20         30         40         50         60 
MAALRAVCSL RGVGAQVLRA GSGIRLPSQP SRGARRWQPD IEWAEQFSGA VMYPSKETAH 

        70         80         90        100        110        120 
WKPPPWNDVD VLKEKVVTNV TLNFGPQHPA AHGVLRLVLE LSGEMVRKCD PHIGLLHRGT 

       130        140        150        160        170        180 
EKLIEYKTYL QALPYFDRLD YVSMMCNEQA YSLAVEKLLN IQPPPRAQWI RVLFGEITRI 

       190        200        210        220        230        240 
LNHIMAVTTH ALDIGAMTPF FWMFEEREKM FEFYERVSGA RMHAAYIRPG GVHQDLPLGL 

       250        260        270        280        290        300 
MDDIYEFSKN FSLRIDEVEE MLTNNRIWRN RTVDIGVVSA EDALNYGFSG VMLRGSGIQW 

       310        320        330        340        350        360 
DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS LRIIEQCLNK MPPGEIKVDD 

       370        380        390        400        410        420 
AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI EAPKGEFGVY LVSDGSSRPY 

       430        440        450        460 
RCKIKAPGFA HLAGLDKMSK GHMLADVVAI IGTQDIVFGE IDR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Brown Norway. Tissue: Testis.
[2]	"Proteome profile of the mature rat olfactory bulb." Maurya D.K., Sundaram C.S., Bhargava P. Proteomics 9:2593-2599(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC082067 mRNA. Translation: AAH82067.1.
IPI	IPI00471647.
RefSeq	NP_001011907.1. NM_001011907.1. XP_003751325.1. XM_003751277.1.
UniGene	Rn.99666.
3D structure databases
HSSP	HSSP built from PDB template 2FUG based on UniProtKB Q56220.
ProteinModelPortal	Q641Y2.
ModBase	Search...
2D gel databases
World-2DPAGE	0004:Q641Y2.
Proteomic databases
PRIDE	Q641Y2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000058423; ENSRNOP00000055224; ENSRNOG00000038372.
GeneID	289218.
KEGG	rno:289218.
UCSC	RGD:1307109. rat.
Organism-specific databases
CTD	4720.
RGD	1307109. Ndufs2.
Phylogenomic databases
GeneTree	ENSGT00390000009529.
HOVERGEN	HBG000760.
KO	K03935.
Gene expression databases
Genevestigator	Q641Y2.
Family and domain databases
InterPro	IPR001135. NADH_Q_OxRdtase_suD. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR022885. NDH1_su_D/H. [Graphical view]
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
TIGRFAMs	TIGR01962. NuoD. 1 hit.
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	629400.

Entry information

Entry name

NDUS2_RAT

Accession

Primary (citable) accession number: Q641Y2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	October 25, 2004
Last modified:	April 3, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents