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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

Gene

Ddost

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-RNO-6798695. Neutrophil degranulation.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit (EC:2.4.99.18)
Short name:
DDOST 48 kDa subunit
Short name:
Oligosaccharyl transferase 48 kDa subunit
Gene namesi
Name:Ddost
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1308970. Ddost.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 412LumenalSequence analysisAdd BLAST384
Transmembranei413 – 432HelicalSequence analysisAdd BLAST20
Topological domaini433 – 441CytoplasmicSequence analysis9

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28By similarityAdd BLAST28
ChainiPRO_000035744729 – 441Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunitAdd BLAST413

Proteomic databases

PaxDbiQ641Y0.
PRIDEiQ641Y0.

PTM databases

iPTMnetiQ641Y0.
PhosphoSitePlusiQ641Y0.
SwissPalmiQ641Y0.

Expressioni

Gene expression databases

ExpressionAtlasiQ641Y0. baseline and differential.
GenevisibleiQ641Y0. RN.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-4655735.
STRINGi10116.ENSRNOP00000062365.

Structurei

3D structure databases

ProteinModelPortaliQ641Y0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDOST 48 kDa subunit family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2754. Eukaryota.
ENOG410XSF3. LUCA.
GeneTreeiENSGT00390000017294.
HOGENOMiHOG000173744.
HOVERGENiHBG053378.
InParanoidiQ641Y0.
KOiK12670.
OMAiAFSMMIG.
OrthoDBiEOG091G07Z5.
PhylomeDBiQ641Y0.

Family and domain databases

InterProiIPR005013. DDOST_48_kDa_subunit.
[Graphical view]
PANTHERiPTHR10830. PTHR10830. 1 hit.
PfamiPF03345. DDOST_48kD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q641Y0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMVPRLAVR AWPLCGLLLA ALGCVCASGP RTLVLLDNLN VRDTHSLFFR
60 70 80 90 100
SLKDRGFELT FKTADDPSLS LIKYGEFLYD NLIIFSPSVE DFGGNINVET
110 120 130 140 150
ISAFIDGGGS VLVAASSDIG DPLRELGSEC GIEFDEEKTA VIDHHNYDVS
160 170 180 190 200
DLGQHTLIVA DTENLLKAPT IVGKSSLNPI LFRGVGMVAD PDNPLVLDIL
210 220 230 240 250
TGSSTSYSFF PDKPITQYPH AVGRNTLLIA GLQARNNARV IFSGSLDFFS
260 270 280 290 300
DAFFNSAVQK AAPGAQRYSQ TGNYELAVAL SRWVFKEEGV LRVGPVSHHR
310 320 330 340 350
VGETAPPNAY TVTDLVEYSI VIEQLSNGKW VPFDGDDIQL EFVRIDPFVR
360 370 380 390 400
TFLKRKGGKY SVQFKLPDVY GVFQFKVDYN RLGYTHLYSS TQVSVRPLQH
410 420 430 440
TQYERFIPSA YPYYASAFSM MAGLFLFSVV FLHMKEKEKS D
Length:441
Mass (Da):48,896
Last modified:October 25, 2004 - v1
Checksum:iDB057B01AA5CB6AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082075 mRNA. Translation: AAH82075.1.
RefSeqiNP_001012104.1. NM_001012104.1.
UniGeneiRn.102842.

Genome annotation databases

EnsembliENSRNOT00000067228; ENSRNOP00000062365; ENSRNOG00000015079.
GeneIDi313648.
KEGGirno:313648.
UCSCiRGD:1308970. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082075 mRNA. Translation: AAH82075.1.
RefSeqiNP_001012104.1. NM_001012104.1.
UniGeneiRn.102842.

3D structure databases

ProteinModelPortaliQ641Y0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4655735.
STRINGi10116.ENSRNOP00000062365.

PTM databases

iPTMnetiQ641Y0.
PhosphoSitePlusiQ641Y0.
SwissPalmiQ641Y0.

Proteomic databases

PaxDbiQ641Y0.
PRIDEiQ641Y0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000067228; ENSRNOP00000062365; ENSRNOG00000015079.
GeneIDi313648.
KEGGirno:313648.
UCSCiRGD:1308970. rat.

Organism-specific databases

CTDi1650.
RGDi1308970. Ddost.

Phylogenomic databases

eggNOGiKOG2754. Eukaryota.
ENOG410XSF3. LUCA.
GeneTreeiENSGT00390000017294.
HOGENOMiHOG000173744.
HOVERGENiHBG053378.
InParanoidiQ641Y0.
KOiK12670.
OMAiAFSMMIG.
OrthoDBiEOG091G07Z5.
PhylomeDBiQ641Y0.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-RNO-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ641Y0.

Gene expression databases

ExpressionAtlasiQ641Y0. baseline and differential.
GenevisibleiQ641Y0. RN.

Family and domain databases

InterProiIPR005013. DDOST_48_kDa_subunit.
[Graphical view]
PANTHERiPTHR10830. PTHR10830. 1 hit.
PfamiPF03345. DDOST_48kD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOST48_RAT
AccessioniPrimary (citable) accession number: Q641Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: October 25, 2004
Last modified: November 30, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.