Reviewed, UniProtKB/Swiss-Prot Q641Y0 (OST48_RAT)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
      Short name=Oligosaccharyl transferase 48 kDa subunit
      Short name=DDOST 48 kDa subunit
    EC=2.4.1.119

Gene names

Name:

Ddost

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane
Molecular function	Transferase
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	By similarity
	Chain	29 – 441	413	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000357447
Regions
	Topological domain	29 – 412	384	Lumenal Potential
	Transmembrane	413 – 432	20	Potential
	Topological domain	433 – 441	9	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q641Y0-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: DB057B01AA5CB6AD	FASTA	441	48,896
	10 20 30 40 50 60 MKMVPRLAVR AWPLCGLLLA ALGCVCASGP RTLVLLDNLN VRDTHSLFFR SLKDRGFELT 70 80 90 100 110 120 FKTADDPSLS LIKYGEFLYD NLIIFSPSVE DFGGNINVET ISAFIDGGGS VLVAASSDIG 130 140 150 160 170 180 DPLRELGSEC GIEFDEEKTA VIDHHNYDVS DLGQHTLIVA DTENLLKAPT IVGKSSLNPI 190 200 210 220 230 240 LFRGVGMVAD PDNPLVLDIL TGSSTSYSFF PDKPITQYPH AVGRNTLLIA GLQARNNARV 250 260 270 280 290 300 IFSGSLDFFS DAFFNSAVQK AAPGAQRYSQ TGNYELAVAL SRWVFKEEGV LRVGPVSHHR 310 320 330 340 350 360 VGETAPPNAY TVTDLVEYSI VIEQLSNGKW VPFDGDDIQL EFVRIDPFVR TFLKRKGGKY 370 380 390 400 410 420 SVQFKLPDVY GVFQFKVDYN RLGYTHLYSS TQVSVRPLQH TQYERFIPSA YPYYASAFSM 430 440 MAGLFLFSVV FLHMKEKEKS D

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC082075 mRNA. Translation: AAH82075.1.
IPI	IPI00471645.
RefSeq	NP_001012104.1.
UniGene	Rn.102842
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q641Y0.
Genome annotation databases
Ensembl	ENSRNOT00000067228; ENSRNOP00000062365; ENSRNOG00000015079; Rattus norvegicus. [Genome view]
GeneID	313648.
KEGG	rno:313648.
Organism-specific databases
CTD	313648.
RGD	1308970. Ddost.
Phylogenomic databases
eggNOG	maNOG09539.
HOVERGEN	Q641Y0.
PhylomeDB	Q641Y0.
Gene expression databases
Genevestigator	Q641Y0.
Family and domain databases
InterPro	IPR005013. DDOST_48kDa. [Graphical view]
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	666597.

Entry information

Entry name

OST48_RAT

Accession

Primary (citable) accession number: Q641Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	October 25, 2004
Last modified:	January 19, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)