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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

Gene

Ddost

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Required for the assembly of both SST3A- and SS3B-containing OST complexes. Required for efficient N-glycosylation.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-RNO-6798695. Neutrophil degranulation.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Short name:
DDOST 48 kDa subunit
Short name:
Oligosaccharyl transferase 48 kDa subunit
Gene namesi
Name:Ddost
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1308970. Ddost.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 412LumenalSequence analysisAdd BLAST384
Transmembranei413 – 432HelicalSequence analysisAdd BLAST20
Topological domaini433 – 441CytoplasmicSequence analysis9

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28By similarityAdd BLAST28
ChainiPRO_000035744729 – 441Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunitAdd BLAST413

Proteomic databases

PaxDbiQ641Y0.
PRIDEiQ641Y0.

PTM databases

iPTMnetiQ641Y0.
PhosphoSitePlusiQ641Y0.
SwissPalmiQ641Y0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000015079.
GenevisibleiQ641Y0. RN.

Interactioni

Subunit structurei

CComponent of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).By similarity

Protein-protein interaction databases

BioGridi260501. 1 interactor.
MINTiMINT-4655735.
STRINGi10116.ENSRNOP00000062365.

Family & Domainsi

Sequence similaritiesi

Belongs to the DDOST 48 kDa subunit family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2754. Eukaryota.
ENOG410XSF3. LUCA.
GeneTreeiENSGT00390000017294.
HOGENOMiHOG000173744.
HOVERGENiHBG053378.
InParanoidiQ641Y0.
KOiK12670.
OMAiAFSMMIG.
OrthoDBiEOG091G07Z5.
PhylomeDBiQ641Y0.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR005013. DDOST_48_kDa_subunit.
PANTHERiPTHR10830. PTHR10830. 1 hit.
PfamiView protein in Pfam
PF03345. DDOST_48kD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q641Y0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMVPRLAVR AWPLCGLLLA ALGCVCASGP RTLVLLDNLN VRDTHSLFFR
60 70 80 90 100
SLKDRGFELT FKTADDPSLS LIKYGEFLYD NLIIFSPSVE DFGGNINVET
110 120 130 140 150
ISAFIDGGGS VLVAASSDIG DPLRELGSEC GIEFDEEKTA VIDHHNYDVS
160 170 180 190 200
DLGQHTLIVA DTENLLKAPT IVGKSSLNPI LFRGVGMVAD PDNPLVLDIL
210 220 230 240 250
TGSSTSYSFF PDKPITQYPH AVGRNTLLIA GLQARNNARV IFSGSLDFFS
260 270 280 290 300
DAFFNSAVQK AAPGAQRYSQ TGNYELAVAL SRWVFKEEGV LRVGPVSHHR
310 320 330 340 350
VGETAPPNAY TVTDLVEYSI VIEQLSNGKW VPFDGDDIQL EFVRIDPFVR
360 370 380 390 400
TFLKRKGGKY SVQFKLPDVY GVFQFKVDYN RLGYTHLYSS TQVSVRPLQH
410 420 430 440
TQYERFIPSA YPYYASAFSM MAGLFLFSVV FLHMKEKEKS D
Length:441
Mass (Da):48,896
Last modified:October 25, 2004 - v1
Checksum:iDB057B01AA5CB6AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082075 mRNA. Translation: AAH82075.1.
RefSeqiNP_001012104.1. NM_001012104.1.
UniGeneiRn.102842.

Genome annotation databases

EnsembliENSRNOT00000067228; ENSRNOP00000062365; ENSRNOG00000015079.
GeneIDi313648.
KEGGirno:313648.
UCSCiRGD:1308970. rat.

Similar proteinsi

Entry informationi

Entry nameiOST48_RAT
AccessioniPrimary (citable) accession number: Q641Y0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: October 25, 2004
Last modified: August 30, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families