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Protein

Eukaryotic translation initiation factor 3 subunit E

Gene

Eif3e

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-RNO-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-RNO-72649 Translation initiation complex formation
R-RNO-72689 Formation of a pool of free 40S subunits
R-RNO-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-RNO-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit EUniRule annotation
Short name:
eIF3eUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 6UniRule annotation
eIF-3 p48UniRule annotation
Gene namesi
Name:Eif3e
Synonyms:Eif3s6, Int6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1311521 Eif3e

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation
ChainiPRO_00002918732 – 445Eukaryotic translation initiation factor 3 subunit EAdd BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineUniRule annotationBy similarity1
Modified residuei399PhosphoserineBy similarity1
Modified residuei439PhosphothreonineBy similarity1
Modified residuei442PhosphoserineBy similarity1
Modified residuei445PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ641X8
PRIDEiQ641X8

PTM databases

iPTMnetiQ641X8
PhosphoSitePlusiQ641X8
SwissPalmiQ641X8

Expressioni

Gene expression databases

BgeeiENSRNOG00000027690
GenevisibleiQ641X8 RN

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with COPS3, COPS6, COPS7 (COPS7A or COPS7B), EIF4G1, EPAS1, MCM7, NCBP1, PSMC6, TRIM27 and UPF2 (By similarity). Interacts with IFIT1 and IFIT2 (By similarity).UniRule annotation

Protein-protein interaction databases

BioGridi256362, 1 interactor
IntActiQ641X8, 1 interactor
STRINGi10116.ENSRNOP00000029790

Structurei

3D structure databases

ProteinModelPortaliQ641X8
SMRiQ641X8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini221 – 398PCIPROSITE-ProRule annotationAdd BLAST178

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 128Sufficient for interaction with EPAS1UniRule annotationAdd BLAST125
Regioni9 – 195Sufficient for interaction with TRIM27UniRule annotationAdd BLAST187
Regioni351 – 445Sufficient for interaction with MCM7UniRule annotationAdd BLAST95

Sequence similaritiesi

Belongs to the eIF-3 subunit E family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2758 Eukaryota
ENOG410XQK5 LUCA
GeneTreeiENSGT00390000002661
HOGENOMiHOG000036697
HOVERGENiHBG001097
InParanoidiQ641X8
KOiK03250
OMAiGFFRTQV
OrthoDBiEOG091G075K
PhylomeDBiQ641X8
TreeFamiTF101518

Family and domain databases

Gene3Di1.10.10.10, 1 hit
HAMAPiMF_03004 eIF3e, 1 hit
InterProiView protein in InterPro
IPR016650 eIF3e
IPR019010 eIF3e_N
IPR000717 PCI_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10317 PTHR10317, 1 hit
PfamiView protein in Pfam
PF09440 eIF3_N, 1 hit
PF01399 PCI, 1 hit
PIRSFiPIRSF016255 eIF3e_su6, 1 hit
SMARTiView protein in SMART
SM01186 eIF3_N, 1 hit
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q641X8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM
60 70 80 90 100
VDFAMDVYKN LYSDDIPHAL REKRTTVVAQ LKQLQAETEP IVKMFEDPET
110 120 130 140 150
TRQMQSTRDG RMLFDYLADK HGFRQEYLDT LYRYAKFQYE CGNYSGAAEY
160 170 180 190 200
LYFFRVLVPA TDRNALSSLW GKLASEILMQ NWDAAMEDLT RLKETIDNNS
210 220 230 240 250
VSSPLQSLQQ RTWLIHWSLF VFFNHPKGRD NIIDLFLYQP QYLNAIQTMC
260 270 280 290 300
PHILRYLTTA VITNKDVRKR RQVLKDLVKV IQQESYTYKD PITEFVECLY
310 320 330 340 350
VNFDFDGAQK KLRECESVLV NDFFLVACLE DFIENARLFI FETFCRIHQC
360 370 380 390 400
ISINMLADKL NMTPEEAERW IVNLIRNARL DAKIDSKLGH VVMGNNAVSP
410 420 430 440
YQQVIEKTKS LSFRSQMLAM NIEKKLNQNS RSEAPNWATQ DSGFY
Length:445
Mass (Da):52,221
Last modified:October 25, 2004 - v1
Checksum:iA5368651DD0DDD0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082087 mRNA Translation: AAH82087.1
RefSeqiNP_001011990.1, NM_001011990.1
XP_017458930.1, XM_017603441.1
UniGeneiRn.98547

Genome annotation databases

EnsembliENSRNOT00000038340; ENSRNOP00000029790; ENSRNOG00000027690
GeneIDi108348260
299872
KEGGirno:108348260
rno:299872
UCSCiRGD:1311521 rat

Similar proteinsi

Entry informationi

Entry nameiEIF3E_RAT
AccessioniPrimary (citable) accession number: Q641X8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: October 25, 2004
Last modified: May 23, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families

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