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Protein

Beta-hexosaminidase subunit alpha

Gene

Hexa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei323 – 3231Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiREACT_287178. CS/DS degradation.
REACT_293890. Hyaluronan uptake and degradation.
REACT_325882. Keratan sulfate degradation.
REACT_330669. Glycosphingolipid metabolism.
SABIO-RKQ641X3.

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidase subunit alpha (EC:3.2.1.52)
Alternative name(s):
Beta-N-acetylhexosaminidase subunit alpha
Short name:
Hexosaminidase subunit A
N-acetyl-beta-glucosaminidase subunit alpha
Gene namesi
Name:Hexa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi2792. Hexa.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Propeptidei23 – 8866By similarityPRO_0000011999Add
BLAST
Chaini89 – 528440Beta-hexosaminidase subunit alphaPRO_0000012000Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi58 ↔ 104By similarity
Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi277 ↔ 328By similarity
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi487 – 4871N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi504 ↔ 521By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ641X3.
PRIDEiQ641X3.

Expressioni

Gene expression databases

GenevisibleiQ641X3. RN.

Interactioni

Protein-protein interaction databases

IntActiQ641X3. 1 interaction.
STRINGi10116.ENSRNOP00000013747.

Structurei

3D structure databases

ProteinModelPortaliQ641X3.
SMRiQ641X3. Positions 23-527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni422 – 4232Critical for hydrolyzis GM2 gangliosidesBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 20 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3525.
GeneTreeiENSGT00390000008107.
HOGENOMiHOG000157972.
HOVERGENiHBG005961.
InParanoidiQ641X3.
KOiK12373.
OMAiANINNIP.
OrthoDBiEOG7KDFB6.
PhylomeDBiQ641X3.
TreeFamiTF313036.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamiPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSiPR00738. GLHYDRLASE20.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q641X3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGCRLWVSL LLAAALACLA TALWPWPQYI QTSHRRYTLY PNNFQFRYHA
60 70 80 90 100
GSAAQAGCVV LDEAFRRYRS LLFGSGSWPR PSFSKKQQPL GKNILMVSVV
110 120 130 140 150
TAECNEFPNL ESVENYTLTI NDDQCLLSSE TVWGALRGLE TFSQLVWKSA
160 170 180 190 200
EGTFFINKTK ITDFPRFPHR GILLDTSRHY LPLSSILNTL DVMAYNKFNV
210 220 230 240 250
FHWHLVDDSS FPYESFTFPE LTRKGSFNPV THIYTAQDVK EVIEYARLRG
260 270 280 290 300
IRVLAEFDTP GHTLSWGAGV PGLLTPCYSG SRLSGTYGPV NPSLNSTYDF
310 320 330 340 350
MSTFFLEISS VFPDFYLHLG GDEVDFTCWK SNPNIQAFMK KKGFTDYKQL
360 370 380 390 400
ESFYIQTLLD IVSDYDKGYV VWQEVFDNKV KVRPDTIIQV WREEMPVQYM
410 420 430 440 450
KEIEAITQAG FRALLSAPWY LNRVKYGPDW KEMYKVEPLA FRGTPAQKAL
460 470 480 490 500
VIGGEACMWG EYVDSTNLVP RLWPRAGAIA ERLWSSNLTT NMDFAFKRLS
510 520
HFRCELLRRG IQAQPISVGY CEQEFEHT
Length:528
Mass (Da):60,538
Last modified:October 25, 2004 - v1
Checksum:iB87DD9A21260DCA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082097 mRNA. Translation: AAH82097.1.
RefSeqiNP_001004443.1. NM_001004443.1.
UniGeneiRn.92939.

Genome annotation databases

EnsembliENSRNOT00000013747; ENSRNOP00000013747; ENSRNOG00000010252.
GeneIDi300757.
KEGGirno:300757.
UCSCiRGD:2792. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082097 mRNA. Translation: AAH82097.1.
RefSeqiNP_001004443.1. NM_001004443.1.
UniGeneiRn.92939.

3D structure databases

ProteinModelPortaliQ641X3.
SMRiQ641X3. Positions 23-527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ641X3. 1 interaction.
STRINGi10116.ENSRNOP00000013747.

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Proteomic databases

PaxDbiQ641X3.
PRIDEiQ641X3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013747; ENSRNOP00000013747; ENSRNOG00000010252.
GeneIDi300757.
KEGGirno:300757.
UCSCiRGD:2792. rat.

Organism-specific databases

CTDi3073.
RGDi2792. Hexa.

Phylogenomic databases

eggNOGiCOG3525.
GeneTreeiENSGT00390000008107.
HOGENOMiHOG000157972.
HOVERGENiHBG005961.
InParanoidiQ641X3.
KOiK12373.
OMAiANINNIP.
OrthoDBiEOG7KDFB6.
PhylomeDBiQ641X3.
TreeFamiTF313036.

Enzyme and pathway databases

ReactomeiREACT_287178. CS/DS degradation.
REACT_293890. Hyaluronan uptake and degradation.
REACT_325882. Keratan sulfate degradation.
REACT_330669. Glycosphingolipid metabolism.
SABIO-RKQ641X3.

Miscellaneous databases

NextBioi647448.
PROiQ641X3.

Gene expression databases

GenevisibleiQ641X3. RN.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamiPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSiPR00738. GLHYDRLASE20.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiHEXA_RAT
AccessioniPrimary (citable) accession number: Q641X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: October 25, 2004
Last modified: July 22, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.