Reviewed, UniProtKB/Swiss-Prot Q641X3 (HEXA_RAT)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase subunit alpha
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase subunit alpha
    Beta-N-acetylhexosaminidase subunit alpha
      Short name=Hexosaminidase subunit A

Gene names

Name:

Hexa

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 88	66	By similarity		PRO_0000011999
	Chain	89 – 528	440	Beta-hexosaminidase subunit alpha		PRO_0000012000
Sites
	Active site	323	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Glycosylation	157	1	N-linked (GlcNAc...) Potential
	Glycosylation	295	1	N-linked (GlcNAc...) Potential
	Glycosylation	487	1	N-linked (GlcNAc...) Potential
	Disulfide bond	58 ↔ 104		By similarity
	Disulfide bond	277 ↔ 328		By similarity
	Disulfide bond	504 ↔ 521		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q641X3-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: B87DD9A21260DCA3	FASTA	528	60,538
	10 20 30 40 50 60 MAGCRLWVSL LLAAALACLA TALWPWPQYI QTSHRRYTLY PNNFQFRYHA GSAAQAGCVV 70 80 90 100 110 120 LDEAFRRYRS LLFGSGSWPR PSFSKKQQPL GKNILMVSVV TAECNEFPNL ESVENYTLTI 130 140 150 160 170 180 NDDQCLLSSE TVWGALRGLE TFSQLVWKSA EGTFFINKTK ITDFPRFPHR GILLDTSRHY 190 200 210 220 230 240 LPLSSILNTL DVMAYNKFNV FHWHLVDDSS FPYESFTFPE LTRKGSFNPV THIYTAQDVK 250 260 270 280 290 300 EVIEYARLRG IRVLAEFDTP GHTLSWGAGV PGLLTPCYSG SRLSGTYGPV NPSLNSTYDF 310 320 330 340 350 360 MSTFFLEISS VFPDFYLHLG GDEVDFTCWK SNPNIQAFMK KKGFTDYKQL ESFYIQTLLD 370 380 390 400 410 420 IVSDYDKGYV VWQEVFDNKV KVRPDTIIQV WREEMPVQYM KEIEAITQAG FRALLSAPWY 430 440 450 460 470 480 LNRVKYGPDW KEMYKVEPLA FRGTPAQKAL VIGGEACMWG EYVDSTNLVP RLWPRAGAIA 490 500 510 520 ERLWSSNLTT NMDFAFKRLS HFRCELLRRG IQAQPISVGY CEQEFEHT

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC082097 mRNA. Translation: AAH82097.1.
IPI	IPI00394353.
RefSeq	NP_001004443.1.
UniGene	Rn.92939
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q641X3.
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
Genome annotation databases
Ensembl	ENSRNOT00000013747; ENSRNOP00000013747; ENSRNOG00000010252; Rattus norvegicus. [Genome view]
GeneID	300757.
KEGG	rno:300757.
NMPDR	fig|10116.3.peg.28740.
UCSC	NM_001004443. rat.
Organism-specific databases
CTD	300757.
RGD	2792. Hexa.
Phylogenomic databases
HOVERGEN	Q641X3.
OMA	TNLVPRL.
Enzyme and pathway databases
BRENDA	3.2.1.52. 248.
Gene expression databases
ArrayExpress	Q641X3.
Genevestigator	Q641X3.
GermOnline	ENSRNOG00000010252. Rattus norvegicus.
Family and domain databases
InterPro	IPR001540. Glyco_hydro_20. IPR015883. Glyco_hydro_20_cat-core. IPR013781. Glyco_hydro_sg_catalytic. IPR015882. HexNAc-like_b. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR22600. Glyco_hydro_20. 1 hit.
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PRINTS	PR00738. GLHYDRLASE20.
ProtoNet	Search...
Other Resources
NextBio	647448.

Entry information

Entry name

HEXA_RAT

Accession

Primary (citable) accession number: Q641X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)