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Protein

RNA polymerase-associated protein LEO1

Gene

Leo1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-RNO-112382. Formation of RNA Pol II elongation complex.
R-RNO-112387. Elongation arrest and recovery.
R-RNO-201722. Formation of the beta-catenin:TCF transactivating complex.
R-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-75955. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase-associated protein LEO1
Gene namesi
Name:Leo1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1549772. Leo1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002478222 – 678RNA polymerase-associated protein LEO1Add BLAST677

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei10PhosphoserineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Modified residuei66PhosphoserineBy similarity1
Modified residuei161PhosphoserineCombined sources1
Modified residuei164PhosphoserineCombined sources1
Modified residuei173PhosphoserineCombined sources1
Modified residuei182PhosphoserineCombined sources1
Modified residuei190PhosphoserineCombined sources1
Modified residuei200PhosphothreonineCombined sources1
Modified residuei209PhosphoserineCombined sources1
Modified residuei217PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1
Modified residuei232PhosphoserineCombined sources1
Modified residuei241PhosphoserineCombined sources1
Modified residuei250PhosphoserineCombined sources1
Modified residuei258PhosphoserineCombined sources1
Modified residuei266PhosphoserineCombined sources1
Modified residuei289PhosphoserineBy similarity1
Modified residuei291PhosphoserineBy similarity1
Modified residuei306PhosphoserineCombined sources1
Modified residuei308PhosphoserineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei618PhosphotyrosineBy similarity1
Modified residuei619PhosphoserineBy similarity1
Modified residuei620PhosphoserineBy similarity1
Modified residuei622PhosphoserineBy similarity1
Modified residuei626PhosphoserineBy similarity1
Modified residuei642PhosphoserineCombined sources1
Modified residuei670PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ641X2.
PRIDEiQ641X2.

PTM databases

iPTMnetiQ641X2.
PhosphoSitePlusiQ641X2.

Expressioni

Gene expression databases

BgeeiENSRNOG00000010116.
GenevisibleiQ641X2. RN.

Interactioni

Subunit structurei

Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61. Interacts with TCEA1, SUPT5H and CTNNB1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013510.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi20 – 341Asp-richAdd BLAST322

Sequence similaritiesi

Belongs to the LEO1 family.Curated

Phylogenomic databases

eggNOGiKOG1181. Eukaryota.
KOG2428. Eukaryota.
ENOG410XRI0. LUCA.
GeneTreeiENSGT00550000074952.
HOGENOMiHOG000253934.
HOVERGENiHBG081913.
InParanoidiQ641X2.
KOiK15177.
OMAiDEGSHHS.
OrthoDBiEOG091G0ILS.
PhylomeDBiQ641X2.
TreeFamiTF321961.

Family and domain databases

InterProiIPR007149. Leo1.
[Graphical view]
PfamiPF04004. Leo1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q641X2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADMEDLFGS EAESEAERKD SDSGSDSDSD QDNGASGSNA SGSESDQDER
60 70 80 90 100
GDSGQPSNKE LFGDDSEEEG APHHSGSDNH SEQSDNRSEA SEERSDHEDN
110 120 130 140 150
EPSDVDQHSG SEAHNDDDDD DDDEDDDDEG HRSDEGSHHS EAEGSEKAQS
160 170 180 190 200
DDEKWDGEDK SDQSDDDEKL QNSDDEEREQ GSDDEKLQNS ADEEEKMQNT
210 220 230 240 250
DDEDRAQLSD DDRQQLSEEE KGNSDDERPA ASDNDEEKQN SDDEDRPQVS
260 270 280 290 300
DEEKMQNSDD ERPQVSDEDR RHSDDEEEQD QKSESARGSD SEDEVLRMKR
310 320 330 340 350
KNAIPSDSEV DSDTEVPKDN NGTMDLFGGA DDISSGSDGE DKPPTPGQPV
360 370 380 390 400
DENGLPQDQQ EEEPIPETRI EVEIPKVNTD LGNDLYFVKL PNFLSVEPRP
410 420 430 440 450
FDPQYYEDEF EDEEMLDEEG RTRLKLKVEN TIRWRMRRDE EGNEIKESNA
460 470 480 490 500
RIVKWSDGSM SLHLGNEVFD VYKAPLQGDH NHLFIRQGTG LQGQAVFKTK
510 520 530 540 550
LTFRPHSTDS ATHRKMTLSL ADRCSKTQKI RILPMAGRDP ECQRTEMIKK
560 570 580 590 600
EEERLRASIR RESQQRRMRE KQHQRGLSAS YLEPDRYDEE EEGEESVSLA
610 620 630 640 650
AIKNRYKGGI REERARIYSS DSDEGSEEDK AQRLLKAKKL NSDEEGESSG
660 670
KRKAEDDDKA NKKHKKYVIS DEEEEEDD
Length:678
Mass (Da):76,957
Last modified:October 25, 2004 - v1
Checksum:i5C213780056A847E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082098 mRNA. Translation: AAH82098.1.
RefSeqiNP_001005548.1. NM_001005548.1.
UniGeneiRn.43195.

Genome annotation databases

EnsembliENSRNOT00000013512; ENSRNOP00000013510; ENSRNOG00000010116.
GeneIDi300837.
KEGGirno:300837.
UCSCiRGD:1549772. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082098 mRNA. Translation: AAH82098.1.
RefSeqiNP_001005548.1. NM_001005548.1.
UniGeneiRn.43195.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013510.

PTM databases

iPTMnetiQ641X2.
PhosphoSitePlusiQ641X2.

Proteomic databases

PaxDbiQ641X2.
PRIDEiQ641X2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013512; ENSRNOP00000013510; ENSRNOG00000010116.
GeneIDi300837.
KEGGirno:300837.
UCSCiRGD:1549772. rat.

Organism-specific databases

CTDi123169.
RGDi1549772. Leo1.

Phylogenomic databases

eggNOGiKOG1181. Eukaryota.
KOG2428. Eukaryota.
ENOG410XRI0. LUCA.
GeneTreeiENSGT00550000074952.
HOGENOMiHOG000253934.
HOVERGENiHBG081913.
InParanoidiQ641X2.
KOiK15177.
OMAiDEGSHHS.
OrthoDBiEOG091G0ILS.
PhylomeDBiQ641X2.
TreeFamiTF321961.

Enzyme and pathway databases

ReactomeiR-RNO-112382. Formation of RNA Pol II elongation complex.
R-RNO-112387. Elongation arrest and recovery.
R-RNO-201722. Formation of the beta-catenin:TCF transactivating complex.
R-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-75955. RNA Polymerase II Transcription Elongation.

Miscellaneous databases

PROiQ641X2.

Gene expression databases

BgeeiENSRNOG00000010116.
GenevisibleiQ641X2. RN.

Family and domain databases

InterProiIPR007149. Leo1.
[Graphical view]
PfamiPF04004. Leo1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEO1_RAT
AccessioniPrimary (citable) accession number: Q641X2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: October 25, 2004
Last modified: November 30, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.