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Protein

Replication factor C subunit 2

Gene

Rfc2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 788ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-110312. Translesion synthesis by REV1.
R-RNO-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-RNO-110320. Translesion Synthesis by POLH.
R-RNO-174411. Polymerase switching on the C-strand of the telomere.
R-RNO-176187. Activation of ATR in response to replication stress.
R-RNO-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-RNO-5655862. Translesion synthesis by POLK.
R-RNO-5656121. Translesion synthesis by POLI.
R-RNO-5656169. Termination of translesion DNA synthesis.
R-RNO-5685938. HDR through Single Strand Annealing (SSA).
R-RNO-5685942. HDR through Homologous Recombination (HRR).
R-RNO-5693607. Processing of DNA double-strand break ends.
R-RNO-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-RNO-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-RNO-5696400. Dual Incision in GG-NER.
R-RNO-6782135. Dual incision in TC-NER.
R-RNO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-RNO-6804756. Regulation of TP53 Activity through Phosphorylation.
R-RNO-69091. Polymerase switching.
R-RNO-69473. G2/M DNA damage checkpoint.

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C subunit 2
Alternative name(s):
Activator 1 subunit C2
Gene namesi
Name:Rfc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi621198. Rfc2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Replication factor C subunit 2PRO_0000330462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei158 – 1581N6-acetyllysineBy similarity
Modified residuei299 – 2991N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ641W4.
PRIDEiQ641W4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000001457.
GenevisibleiQ641W4. RN.

Interactioni

Subunit structurei

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA. RFC2 also interacts with PRKAR1A; the complex may be involved in cell survival (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001989.

Structurei

3D structure databases

ProteinModelPortaliQ641W4.
SMRiQ641W4. Positions 30-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the activator 1 small subunits family.Curated

Phylogenomic databases

eggNOGiKOG0991. Eukaryota.
COG0470. LUCA.
GeneTreeiENSGT00550000075050.
HOGENOMiHOG000224155.
HOVERGENiHBG002053.
InParanoidiQ641W4.
KOiK10755.
OMAiTHSGFGY.
OrthoDBiEOG091G0CKT.
PhylomeDBiQ641W4.
TreeFamiTF300585.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF08542. Rep_fac_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q641W4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVQESGSGS AESGTQEPSP VPSKTTGHYE LPWVEKYRPV KLNEIVGNED
60 70 80 90 100
TVSRLEVFAR EGNVPNIIIA GPPGTGKTTS ILCLARALLG PALKDAVLEL
110 120 130 140 150
NASNDRGIDV VRNKIKMFAQ QKVTLPKGRH KIIILDEADS MTDGAQQALR
160 170 180 190 200
RTMEIYSKTT RFALACNASD KIIEPIQSRC AVLRYTKLTD AQVLSRLMNV
210 220 230 240 250
IEKEKVPYTD DGLEAIIFTA QGDMRQALNN LQSTFSGFGY INSENVFKVC
260 270 280 290 300
DEPHPLLVKE MIQHCVDANI DEAYKILAHL WHLGYSPEDV IGNIFRVCKT
310 320 330 340
FPMAEYLKLE FIKEIGYTHM KVAEGVNSLL QMAGLLARLC QKTMAPVAS
Length:349
Mass (Da):38,657
Last modified:October 25, 2004 - v1
Checksum:i0E9EDF01AFDB2092
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082110 mRNA. Translation: AAH82110.1.
RefSeqiNP_446238.1. NM_053786.1.
UniGeneiRn.113319.

Genome annotation databases

EnsembliENSRNOT00000001989; ENSRNOP00000001989; ENSRNOG00000001457.
GeneIDi116468.
KEGGirno:116468.
UCSCiRGD:621198. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082110 mRNA. Translation: AAH82110.1.
RefSeqiNP_446238.1. NM_053786.1.
UniGeneiRn.113319.

3D structure databases

ProteinModelPortaliQ641W4.
SMRiQ641W4. Positions 30-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001989.

Proteomic databases

PaxDbiQ641W4.
PRIDEiQ641W4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001989; ENSRNOP00000001989; ENSRNOG00000001457.
GeneIDi116468.
KEGGirno:116468.
UCSCiRGD:621198. rat.

Organism-specific databases

CTDi5982.
RGDi621198. Rfc2.

Phylogenomic databases

eggNOGiKOG0991. Eukaryota.
COG0470. LUCA.
GeneTreeiENSGT00550000075050.
HOGENOMiHOG000224155.
HOVERGENiHBG002053.
InParanoidiQ641W4.
KOiK10755.
OMAiTHSGFGY.
OrthoDBiEOG091G0CKT.
PhylomeDBiQ641W4.
TreeFamiTF300585.

Enzyme and pathway databases

ReactomeiR-RNO-110312. Translesion synthesis by REV1.
R-RNO-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-RNO-110320. Translesion Synthesis by POLH.
R-RNO-174411. Polymerase switching on the C-strand of the telomere.
R-RNO-176187. Activation of ATR in response to replication stress.
R-RNO-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-RNO-5655862. Translesion synthesis by POLK.
R-RNO-5656121. Translesion synthesis by POLI.
R-RNO-5656169. Termination of translesion DNA synthesis.
R-RNO-5685938. HDR through Single Strand Annealing (SSA).
R-RNO-5685942. HDR through Homologous Recombination (HRR).
R-RNO-5693607. Processing of DNA double-strand break ends.
R-RNO-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-RNO-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-RNO-5696400. Dual Incision in GG-NER.
R-RNO-6782135. Dual incision in TC-NER.
R-RNO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-RNO-6804756. Regulation of TP53 Activity through Phosphorylation.
R-RNO-69091. Polymerase switching.
R-RNO-69473. G2/M DNA damage checkpoint.

Miscellaneous databases

PROiQ641W4.

Gene expression databases

BgeeiENSRNOG00000001457.
GenevisibleiQ641W4. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF08542. Rep_fac_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRFC2_RAT
AccessioniPrimary (citable) accession number: Q641W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 25, 2004
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.